this is also my experience. However, above pH 8, the buffering effect of the
Jeffamines' amino groups starts to be significant and (rough) pre-adjusting of
the pH might be necessary.
Clemens
Quoting Yogesh Gupta PhD [EMAIL PROTECTED]:
Hi Amit,
Changing the pH of Jeffamine is not so easy . I would keep the pH of
Jeffamine constant and rather change the pH of buffer. Anyway if Jeffamine
concentration is too low as compare to the buffer you are using, it would
not change the final pH much. This is what my experience with Jeffamine is.
Cheers,
Yogesh
On Mon, Apr 21, 2008 at 9:20 AM, amit sharma [EMAIL PROTECTED] wrote:
Dear CCP4ers,
Apologies for a non-CCP4 query. I intend to set up an optimization grid
around one of the crystallization conditions (containing Jeffamine ED-2001pH
7.0 and HEPES pH 7.0) by varying the buffer pH from 5.5 to 7.5. In such a
case, is it recommmended that the pH of Jeffamine ED-2001(pH 7.0) also be
changed to match the different buffer pHs(5.5-7.5)?
Cheers,
Amit
--
Yogesh K. Gupta, Ph.D.
Post Doctoral Fellow
Molecular Physiology Biophysics
Mount Sinai School of Medicine
1425, Madison Av. (East Bldg. 16-26)
Box 1677, New York, NY 10029-6574
Tel:+1 212-659-8639
Fax:+1 212-849-2456
E-mail: [EMAIL PROTECTED]
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