Re: [ccp4bb] tetrameric tag for extracellular proteins

2020-09-26 Thread Srivastava, Dhiraj 
P53 tetramerization domain might be a way to go.


From: CCP4 bulletin board  on behalf of Comolettis 
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Sent: Friday, September 25, 2020 9:59 PM
To: CCP4BB@JISCMAIL.AC.UK 
Subject: [ccp4bb] tetrameric tag for extracellular proteins

Hi all,

Following what someone recently suggested, Fc tag is a great way to dimerize a 
secreted protein. I have successfully tried the TNF receptor-associated factor 
1 tail to trimerize and the COMP (cartilage oligomeric matrix protein) to 
pentamerize my monomer but I can't identify a suitable tag to tetramerize my 
construct.
Any pointer?

Thanks,
Davide Comoletti
VUW, Wellington, NZ



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[ccp4bb] tetrameric tag for extracellular proteins

2020-09-25 Thread Comolettis 
Hi all,

Following what someone recently suggested, Fc tag is a great way to
dimerize a secreted protein. I have successfully tried the TNF
receptor-associated factor 1 tail to *trimerize *and the COMP (cartilage
oligomeric matrix protein) to *pentamerize *my monomer but I can't identify
a suitable tag to *tetramerize *my construct.
Any pointer?

Thanks,
Davide Comoletti
VUW, Wellington, NZ



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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