Re: [ccp4bb] Butandiol as cryopotectant
2,3-BD users, What sort of concentrations are you using? Dave -- Delivered via an Android. On Mar 22, 2010 11:45 PM, Steve Tuske tu...@cabm.rutgers.edu wrote: Hi Ulrike, 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it mostly seems to decrease mosaicity. I was able to switch from a three-step soaking protocol with glycerol and glucose to a quick (30 second to one minute) soak with 2,3-BD. It's a winner. Steve I Hello Ulrike, in our lab 2,3-Butanediol has had a good reputation. Tim On Mon, Mar 22...
Re: [ccp4bb] Butandiol as cryopotectant
Hello Dave, I have good experience with concentrations between 10% and 15% 2,3-butanediol. My typical starting concentration is 12.5%. Good luck. Eike Am 23.03.10 08:27 schrieb David Briggs unter drdavidcbri...@gmail.com: 2,3-BD users, What sort of concentrations are you using? Dave -- Delivered via an Android. On Mar 22, 2010 11:45 PM, Steve Tuske tu...@cabm.rutgers.edu wrote: Hi Ulrike, 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it mostly seems to decrease mosaicity. I was able to switch from a three-step soaking protocol with glycerol and glucose to a quick (30 second to one minute) soak with 2,3-BD. It's a winner. Steve I Hello Ulrike, in our lab 2,3-Butanediol has had a good reputation. Tim On Mon, Mar 22...
Re: [ccp4bb] Butandiol as cryopotectant
I would not rely on recipes. When I pick a cryo protectant, I always prepare a set of solutions and take an image from a large loop dipped into solution. Then I pick the one with the least concentration that does not show ice rings anymore. The behaviour can change with a crystal in the loop, but I hope by using a large loop and taking a big drop I'm on the safe side. For 2,3-butanediol I'd probably prepare the mother liquor with 10%, 15%, 20% 2,30butanediol. Having read what Eike just replied I might include the 2.5%-steps, too. Tim On Tue, Mar 23, 2010 at 07:27:28AM +, David Briggs wrote: 2,3-BD users, What sort of concentrations are you using? Dave -- Delivered via an Android. On Mar 22, 2010 11:45 PM, Steve Tuske tu...@cabm.rutgers.edu wrote: Hi Ulrike, 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it mostly seems to decrease mosaicity. I was able to switch from a three-step soaking protocol with glycerol and glucose to a quick (30 second to one minute) soak with 2,3-BD. It's a winner. Steve I Hello Ulrike, in our lab 2,3-Butanediol has had a good reputation. Tim On Mon, Mar 22... -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A signature.asc Description: Digital signature
Re: [ccp4bb] Butandiol as cryopotectant
20 percent (v/v). Steve 2,3-BD users, What sort of concentrations are you using? Dave -- Delivered via an Android. On Mar 22, 2010 11:45 PM, Steve Tuske tu...@cabm.rutgers.edu wrote: Hi Ulrike, 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it mostly seems to decrease mosaicity. I was able to switch from a three-step soaking protocol with glycerol and glucose to a quick (30 second to one minute) soak with 2,3-BD. It's a winner. Steve I Hello Ulrike, in our lab 2,3-Butanediol has had a good reputation. Tim On Mon, Mar 22...
Re: [ccp4bb] Butandiol as cryopotectant
Hello Ulrike, in our lab 2,3-Butanediol has had a good reputation. Tim On Mon, Mar 22, 2010 at 01:32:33PM +, Ulrike Demmer wrote: Hi everybody, has anyone experience with Butandiol and its different isoforms as cryoprotectant ? Thanks, Ulrike -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A signature.asc Description: Digital signature
Re: [ccp4bb] Butandiol as cryopotectant
Hi Ulrike, 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it mostly seems to decrease mosaicity. I was able to switch from a three-step soaking protocol with glycerol and glucose to a quick (30 second to one minute) soak with 2,3-BD. It's a winner. Steve I Hello Ulrike, in our lab 2,3-Butanediol has had a good reputation. Tim On Mon, Mar 22, 2010 at 01:32:33PM +, Ulrike Demmer wrote: Hi everybody, has anyone experience with Butandiol and its different isoforms as cryoprotectant ? Thanks, Ulrike -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
