Re: [ccp4bb] N-terminal sequencing
I think that this CRO can help you: Proteos 4717 Campus Drive Kalamazoo, Michigan 49008 269.372.3423 http://www.proteos.net Good luck! Bryan From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Junyu Xiao Sent: Tuesday, February 08, 2011 2:52 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] N-terminal sequencing Hi, Sorry for non-crystallography related questions. I am seeking protein N-terminal sequencing service. However, the facilities I have worked with previously (Michigan state and UCSD) were both closed. Does anyone know any companies or core facilities that can do this? Thanks, Junyu --- Junyu Xiao, Ph.D. University of California, San Diego Leichtag Room 283 9500 Gilman Drive, 0721 La Jolla, CA 92093-0721 Lab phone: 858-822-0684 -- Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful.
Re: [ccp4bb] N-terminal sequencing
Dear Daniel, You can get it here as well... University of Pittsburgh Department of Biological Sciences Fifth and Ruskin Aves Clapp Hall 301 Pittsburgh PA 15260 email: hem...@pitt.edu ph: 412.624.0106 or Jack Presley Laboratory Manager UC Davis Molecular Structure Facility http://msf.ucdavis.edu (530) 752-7327 With regards PAnkaj Thanks, On Wed, Feb 9, 2011 at 2:02 AM, Daniel Bonsor wrote: > We have used Alphalyse (http://www.alphalyse.com/picknpost.html). > > Dan >
Re: [ccp4bb] N-terminal sequencing
We have used Alphalyse (http://www.alphalyse.com/picknpost.html). Dan
Re: [ccp4bb] N-terminal sequencing
A colleague here has had good results with the facility at Iowa state: http://www.biotech.iastate.edu/service_facilities/protein.html If you just want to identify the protein, mass spec may be cheaper. The same place will do that, or we have had excellent results with http://www.appliedbiomics.com/ If you need to confirm N-term construct, Edman degradation is probably still more cost effective. Junyu Xiao wrote: Hi, Sorry for non-crystallography related questions. I am seeking protein N-terminal sequencing service. However, the facilities I have worked with previously (Michigan state and UCSD) were both closed. Does anyone know any companies or core facilities that can do this? Thanks, Junyu --- Junyu Xiao, Ph.D. University of California, San Diego Leichtag Room 283 9500 Gilman Drive, 0721 La Jolla, CA 92093-0721 Lab phone: 858-822-0684
Re: [ccp4bb] N-terminal sequencing
Just one more, which we are now using.. The protein analysis facility at University of Giessen offers a range of services to academic and non-academic customers: http://www.uni-giessen.de/cms/fbz/fb11/institute/biochemie/forschung/protein-analytik/gebuhrenubersicht-und-anforderungsformulare They offer good rates for academic customers and we are pleased with our first results. All the best, Tim -Original Message- From: CCP4 bulletin board on behalf of Andreas Förster Sent: Fri 16.04.2010 11:25 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] N-terminal sequencing Beyond these (posted three weeks ago), I don't know any. http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg15004.html I use Jeff Keen's service, which very reliable and adaptable. Andreas On 16/04/2010 9:23, "F.Xavier Gomis-Rüth" wrote: > Dear CCP4ers, > does anybody know of a company that accurately but affordably makes > N-terminal sequencing ? > Thanks a lot in advance to everybody. > Best regards, > Xavier > -- -- Andreas Förster, Research Associate Paul Freemont & Xiaodong Zhang Labs Department of Biochemistry, Imperial College London http://www.msf.bio.ic.ac.uk
Re: [ccp4bb] N-terminal sequencing
Beyond these (posted three weeks ago), I don't know any. http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg15004.html I use Jeff Keen's service, which very reliable and adaptable. Andreas On 16/04/2010 9:23, "F.Xavier Gomis-Rüth" wrote: Dear CCP4ers, does anybody know of a company that accurately but affordably makes N-terminal sequencing ? Thanks a lot in advance to everybody. Best regards, Xavier -- -- Andreas Förster, Research Associate Paul Freemont & Xiaodong Zhang Labs Department of Biochemistry, Imperial College London http://www.msf.bio.ic.ac.uk
