Hi All,
I am a graduate student at the University of Vermont working on a
mycorrhizal ecology project. I have encountered an interesting
'problem' that I am currently trying to resolve and I would appreciate
any input.
I have been staining roots of grasses to quantify infection status. I
have used two stains: ink and trypan blue. They provide very
different results. Does anyone know if ink preferentially binds to
mycorrhizal structures whereas trypan blue is a more general stain,
picking up all fungal structures or if trypan blue is a better overall
stain picking up mycorrhizal structures that ink misses? If so, I
would appreciate any input and pointers to the literature on the
efficacy and specificity of these two stains.
Thanks in advance,
Renee
