Re: [Freesurfer] dwi_motion Tracula

2014-07-23 Thread Anastasia Yendiki


Hi Cat - I'll have to see your trac-all.log, I can't tell what is going on 
just from that one line.


a.y

On Tue, 22 Jul 2014, Cat Chong wrote:


 
Dear Anastasia,
 
With the new Tracula update installed, I ran the command:
 trac-all –qa –c dmrirc_file  with location of bval specified and received
the following error after ‘Loading volume-to-baseline affine
transformations’
 
Could not open ../dmri/ dwi.ecclog for reading.
 
It seems like all subjects do have the dwi.ecclog in their /dmri/
directories.
 
Any ideas of what might have gone wrong?
Cheers,
Cat


On Monday, July 21, 2014 1:53 PM, Anastasia Yendiki
ayend...@nmr.mgh.harvard.edu wrote:



Hi Cat - Just install the update and run trac-all -qa. This will produce
the motion measures. Check the tutorial for where to find the output.

a.y

On Mon, 21 Jul 2014, Cat Chong wrote:

 Hello Anastasia,

  I ran Tracula on Freesurfer 5.3 but without the 2013 Tracula updates.
Now,
 I would like to use the command dwi_motion on my data. I have installed
 the updates, but will I have to completely reprocess my data inducing
steps
 trac-all -prep, trace-all -bedp, and trac-all path in order to use
 dwi_motion? 

 cheers,
 Cat



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Re: [Freesurfer] Looking at dtifit_V1 in freeview

2014-07-23 Thread Anastasia Yendiki

Hi Emily - Actually, there is a bug in the 5.3 version of freeview in that 
specific feature. But you can use fslview - you select the V1 file, click 
on the (i) button and choose Display as: Lines.

a.y

On Tue, 22 Jul 2014, ebell...@uwm.edu wrote:

 Hi all,

 I was wondering how you go about looking at dtifit_V1 (the primary 
 eigenvector of the tensors) displayed as lines in freeview?

 Emily
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Re: [Freesurfer] Pathstat.byvoxel.txt question

2014-07-23 Thread Anastasia Yendiki

Hi Sarina - It's linear interpolation.

a.y

On Wed, 23 Jul 2014, Sarina Sacco wrote:

 
 Hi all freesurfer experts ,
 I have a question about the command trac-all-stat-c ,what is the
 interpolation that uses?
 
 

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[Freesurfer] Diffusion analysis qs

2014-07-23 Thread Rotem Saar
Dear Anastasia  Michael,

Thank u for your answers (I attached my last email and your answers at the
end of this email.

I still want to specify my question. If I only want to extract FA values
using the following script (see script below), and I know that an angle was
applied during the dMRI scans, should I use the rotated gradient matrix ?

When I first analyzed 20 subjects with the gradient matrix that is
published for a T3 Philips magnet (I also read the gradients from the dicom
header - they were the same) I got reasonable results - but now I don't
know what to think of them...
__

source ~/Desktop/fs_script

dt_recon --i /usr/local/freesurfer/subjects/Anatomy0207/DTI/I160.dcm --s
Anatomy0207 --o /usr/local/freesurfer/subjects/Anatomy0207/DTI --b
/usr/local/freesurfer/subjects/Anatomy0207/DTI/bvals.bval
/usr/local/freesurfer/subjects/Anatomy0207/DTI/bvecs.bvec

To check results, run:

tkregister2 --mov /usr/local/freesurfer/subjects/Anatomy0207/DTI/lowb.nii
--reg /usr/local/freesurfer/subjects/Anatomy0207/DTI/register.dat --surf

tkregister2 --s fsaverage --surf white --reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/mask-tal.nii.reg --mov
/usr/local/freesurfer/subjects/Anatomy0207/DTI/mask-tal.nii

tkregister2 --s fsaverage --surf white --reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-tal.nii.reg --mov
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-tal.nii

freeview -v /usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii
/usr/local/freesurfer/subjects/Anatomy0207/DTI/adc.nii

freeview -v /usr/local/freesurfer/subjects/Anatomy0207/mri/brain.mgz
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii:reg=register.dat:colormap=heat:heatscale=.2,.2,1

tkmedit Anatomy0207 orig.mgz -aux brain.mgz -seg wmparc.mgz -reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/register.dat -overlay
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii -fthresh 0.2 -fmax 1

mri_vol2vol --mov /usr/local/freesurfer/subjects/Anatomy0207/DTI/lowb.nii \
--targ
/usr/local/freesurfer/subjects/Anatomy0207/mri/wmparc.mgz \
--inv --interp nearest --o
/usr/local/freesurfer/subjects/Anatomy0207/mri/wmparc2diff.mgz \
--reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/register.dat --no-save-reg

mri_vol2vol --mov /usr/local/freesurfer/subjects/Anatomy0207/DTI/lowb.nii \
--targ
/usr/local/freesurfer/subjects/Anatomy0207/mri/aparc+aseg.mgz \
--inv --interp nearest --o
/usr/local/freesurfer/subjects/Anatomy0207/mri/aparc+aseg2diff.mgz \
--reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/register.dat --no-save-reg

freeview -v /usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii \

/usr/local/freesurfer/subjects/Anatomy0207/mri/aparc+aseg2diff.mgz:colormap=lut


mri_mask /usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii \
 /usr/local/freesurfer/subjects/Anatomy0207/mri/wmparc2diff.mgz \
 /usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-masked.mgz


freeview -v /usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-masked.mgz
/usr/local/freesurfer/subjects/Anatomy0207/mri/aparc+aseg2diff.mgz:colormap=lut

mri_segstats --seg
/usr/local/freesurfer/subjects/Anatomy0207/mri/wmparc2diff.mgz --ctab
$FREESURFER_HOME/FreeSurferColorLUT.txt --i
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-masked.mgz --sum
/usr/local/freesurfer/subjects/Anatomy0207/stats/all_stats_fa-masked

__

It'd be nice if things were that simple, but!

Although the pre-processing is done with FSL tools, tractography is done
with locally written code. Some gradient sets may appear to work with FSL,
but they won't work for us. For example, some dicom converters may perform
a L-R flip as preparation for feeding the gradient vectors into FSL. We
need the unflipped vectors because, instead of flipping vectors, we
convert both vectors and images to LAS orientation. This ensures that they
work both for FSL and for us, and that you can view things correctly in
both freeview and fslview, without the subject looking like it's hanging
upside down from its bat cave or some such awkward arrangement.

In the end, the best way of knowing that the gradient table is correct is
to run one example subject through and view the eigenvectors of the
tensors as lines to see if they're pointing in the right direction. (Even
though the tensors themselves aren't used for tractography, they're good
for troubleshooting.)

On Mon, 21 Jul 2014, Harms, Michael wrote:


 FS uses FSL tools under the hood, so you want to use the bvecs that are
 rotated into the axes of the scan.

 cheers,
 -MH

 --?
 Michael Harms, Ph.D.
 ---
 Conte Center for the Neuroscience of Mental Disorders
 Washington University School of Medicine
 Department of Psychiatry, Box 8134
 

Re: [Freesurfer] Diffusion analysis qs

2014-07-23 Thread Anastasia Yendiki


Hi Rotem - The FA values are actually invariant to a rotation of the 
gradient vectors. You can test that yourself. If, however, you get 
different FA maps with the different gradient tables, then something else 
is going on and not a simple rotation.


a.y

On Wed, 23 Jul 2014, Rotem Saar wrote:


Dear Anastasia  Michael,

Thank u for your answers (I attached my last email and your answers at the
end of this email.

I still want to specify my question. If I only want to extract FA values
using the following script (see script below), and I know that an angle was
applied during the dMRI scans, should I use the rotated gradient matrix ?

When I first analyzed 20 subjects with the gradient matrix that is published
for a T3 Philips magnet (I also read the gradients from the dicom header -
they were the same) I got reasonable results - but now I don't know what to
think of them...
___
___

source ~/Desktop/fs_script

dt_recon --i /usr/local/freesurfer/subjects/Anatomy0207/DTI/I160.dcm --s
Anatomy0207 --o /usr/local/freesurfer/subjects/Anatomy0207/DTI --b
/usr/local/freesurfer/subjects/Anatomy0207/DTI/bvals.bval
/usr/local/freesurfer/subjects/Anatomy0207/DTI/bvecs.bvec

To check results, run:

tkregister2 --mov /usr/local/freesurfer/subjects/Anatomy0207/DTI/lowb.nii
--reg /usr/local/freesurfer/subjects/Anatomy0207/DTI/register.dat --surf

tkregister2 --s fsaverage --surf white --reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/mask-tal.nii.reg --mov
/usr/local/freesurfer/subjects/Anatomy0207/DTI/mask-tal.nii

tkregister2 --s fsaverage --surf white --reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-tal.nii.reg --mov
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-tal.nii

freeview -v /usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii
/usr/local/freesurfer/subjects/Anatomy0207/DTI/adc.nii

freeview -v 
/usr/local/freesurfer/subjects/Anatomy0207/mri/brain.mgz/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii:reg=register.dat:colo
rmap=heat:heatscale=.2,.2,1

tkmedit Anatomy0207 orig.mgz -aux brain.mgz -seg wmparc.mgz -reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/register.dat -overlay
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii -fthresh 0.2 -fmax 1

mri_vol2vol --mov /usr/local/freesurfer/subjects/Anatomy0207/DTI/lowb.nii \
    --targ /usr/local/freesurfer/subjects/Anatomy0207/mri/wmparc.mgz
\
    --inv --interp nearest --o
/usr/local/freesurfer/subjects/Anatomy0207/mri/wmparc2diff.mgz \
    --reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/register.dat --no-save-reg

mri_vol2vol --mov /usr/local/freesurfer/subjects/Anatomy0207/DTI/lowb.nii \
    --targ
/usr/local/freesurfer/subjects/Anatomy0207/mri/aparc+aseg.mgz \
    --inv --interp nearest --o
/usr/local/freesurfer/subjects/Anatomy0207/mri/aparc+aseg2diff.mgz \
    --reg
/usr/local/freesurfer/subjects/Anatomy0207/DTI/register.dat --no-save-reg

freeview -v /usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii \
   
/usr/local/freesurfer/subjects/Anatomy0207/mri/aparc+aseg2diff.mgz:colormap
=lut


mri_mask /usr/local/freesurfer/subjects/Anatomy0207/DTI/fa.nii \
 /usr/local/freesurfer/subjects/Anatomy0207/mri/wmparc2diff.mgz \
 /usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-masked.mgz


freeview -v 
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-masked.mgz/usr/local/freesurfer/subjects/Anatomy0207/mri/aparc+aseg2diff.mgz:colormap
=lut

mri_segstats --seg
/usr/local/freesurfer/subjects/Anatomy0207/mri/wmparc2diff.mgz --ctab
$FREESURFER_HOME/FreeSurferColorLUT.txt --i
/usr/local/freesurfer/subjects/Anatomy0207/DTI/fa-masked.mgz --sum
/usr/local/freesurfer/subjects/Anatomy0207/stats/all_stats_fa-masked

___
___

It'd be nice if things were that simple, but!

Although the pre-processing is done with FSL tools, tractography is done
with locally written code. Some gradient sets may appear to work with FSL,
but they won't work for us. For example, some dicom converters may perform
a L-R flip as preparation for feeding the gradient vectors into FSL. We
need the unflipped vectors because, instead of flipping vectors, we
convert both vectors and images to LAS orientation. This ensures that they
work both for FSL and for us, and that you can view things correctly in
both freeview and fslview, without the subject looking like it's hanging
upside down from its bat cave or some such awkward arrangement.

In the end, the best way of knowing that the gradient table is correct is
to run one example subject through and view the eigenvectors of the
tensors as lines to see if they're pointing in the right direction. (Even
though the tensors themselves aren't used for tractography, they're good
for troubleshooting.)

On Mon, 21 Jul 2014, Harms, Michael wrote:


 FS uses FSL tools under the 

Re: [Freesurfer] Pathstat.byvoxel.txt question

2014-07-23 Thread Sarina Sacco
Thank you for help.
Il 23/lug/2014 10:19 Anastasia Yendiki ayend...@nmr.mgh.harvard.edu ha
scritto:


 Hi Sarina - It's linear interpolation.

 a.y

 On Wed, 23 Jul 2014, Sarina Sacco wrote:

 
  Hi all freesurfer experts ,
  I have a question about the command trac-all-stat-c ,what is the
  interpolation that uses?
 
 
 
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[Freesurfer] running freesurfer subcortical segmentation on a T2 weighted image only ?

2014-07-23 Thread Vincent Noblet
Dear all,

I was wondering if there is a way to use the segmentation of freesurfer 
on a T2 weighted image only (without a T1 weighted image).

Sincerely

Vincent Noblet
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Re: [Freesurfer] bad freesurfer segmentation

2014-07-23 Thread Doety Prins
Just for the record, I accidentally e-mailed you that the slice thickness was 2 
mm, I now found out that the slice thickness is just 1 mm.

Doety
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Re: [Freesurfer] lgi for longitudinal scans

2014-07-23 Thread Anna Jonsson
hi Martin, I just tried this and it resulted in errors/ Do I not have to
specify a flag for the baseid or for each subject, eg -s?

What I did (according to your suggestion)

recon-all -long XXX_1.long XXX_2.long -localGI

This did not work? Do you know why potentially?


On Wed, Mar 26, 2014 at 3:28 PM, Martin Reuter mreu...@nmr.mgh.harvard.edu
wrote:

  Hi Anna,

 no, you would still do:

 recon-all -long tpid baseid -localGI

 just not use the '-all'.

 Best, Martin




 On 03/26/2014 11:12 AM, Anna Jonsson wrote:

 thank you. But if the lnog process has already been done and these
 induviduals already have long directories so to speak, I would just put the
 lgi flag on as normal but just replace the subject name with the long
 subject name eg ?

 recon-all -s _long -localGI


 Thank you



 On Sat, Mar 15, 2014 at 2:07 PM, Martin Reuter 
 mreu...@nmr.mgh.harvard.edu wrote:

 Hi Anna,

  for the image processing, you just add the lgi flag to the regular
 longitudinal recon-all command
 recon-all -long …… -lgi

  for the analysis after that you use the long directories.

  Best, Martin

   On Mar 15, 2014, at 10:04 AM, Anna Jonsson ajonsso...@gmail.com
 wrote:

 Dear group,

  If I want conduct gyrification analyses on the long runs that have been
 through the longitudinal stream, do I just perform the normal baseline
 command on the .long data?

  thank you,

 kind wishes

 Anna
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   -
 Dr. Martin Reuter
 Assistant in Neuroscience - Massachusetts General Hospital
 Instructor in Neurology   - Harvard Medical School
 MGH / HMS / MIT

 A.A.Martinos Center for Biomedical Imaging
 149 Thirteenth Street, Suite 2301
 Charlestown, MA 02129

 Phone: +1-617-724-5652
 Email:
mreu...@nmr.mgh.harvard.edu
reu...@mit.edu
 Web  : http://reuter.mit.edu


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 --
 Martin Reuter, Ph.D.

 Instructor in Neurology
   Harvard Medical School
 Assistant in Neuroscience
   Dept. of Radiology, Massachusetts General Hospital
   Dept. of Neurology, Massachusetts General Hospital
 Research Affiliate
   Computer Science and Artificial Intelligence Lab,
   Dept. of Electrical Engineering and Computer Science,
   Massachusetts Institute of Technology

 A.A.Martinos Center for Biomedical Imaging
 149 Thirteenth Street, Suite 2301
 Charlestown, MA 02129

 Phone: +1-617-724-5652

 Email:
mreu...@nmr.mgh.harvard.edu
reu...@mit.edu
 Web  : http://reuter.mit.edu


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Re: [Freesurfer] running freesurfer subcortical segmentation on a T2 weighted image only ?

2014-07-23 Thread Bruce Fischl
Hi Vincent

you mean to create it from a T2? Not really in our distributed version, 
although Koen Van Leemput (ccd) has some tools that can do this I believe.

cheers
Bruce


On Wed, 23 Jul 2014, Vincent Noblet wrote:

 Dear all,

 I was wondering if there is a way to use the segmentation of freesurfer
 on a T2 weighted image only (without a T1 weighted image).

 Sincerely

 Vincent Noblet
 ___
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 Freesurfer@nmr.mgh.harvard.edu
 https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer



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The information in this e-mail is intended only for the person to whom it is
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Re: [Freesurfer] recon-all error

2014-07-23 Thread Bruce Fischl
Hi Cheng

do the cross-sectional runs look okay? The base? If so, does the first 
intensity normalized images look ok (the T1.mgz and the nu.mgz)? If you 
can't track it down, upload the subject and we will take a look

cheers
Bruce

On Wed, 23 
Jul 2014, Cheng Peng wrote:

 Hi, Freesurfer experts:
 
 
 
 I encountered an error message while i was attempting to run a longitudinal
 study (following the instructions from
 https://surfer.nmr.mgh.harvard.edu/fswiki/LongitudinalProcessing, step 2
 under workflow summary)
 
 egister_mri: find_optimal_transform
 find_optimal_transform: nsamples 2185, passno 0, spacing 8
 GCAhistoScaleImageIntensities: could not find wm peak
 resetting wm mean[0]: 102 -- 107
 resetting gm mean[0]: 64 -- 64
 input volume #1 is the most T1-like
 using real data threshold=0.0
 skull bounding box = (0, 0, 0) -- (255, 255, 255)
 using (85, 85, 128) as brain centroid...
 mean wm in atlas = 107, using box (53,53,96) -- (116, 116,159) to find MRI
 wm
 WARNING: gca.c::GCAhistoScaleImageIntensities: h_mri-nbins=1, mri_peak=-1
 before smoothing, mri peak at 0
 WARNING2: gca.c::GCAhistoScaleImageIntensities: h_mri-nbins=1, mri_peak=-1
 after smoothing, mri peak at 0, scaling input intensities by inf
 
 It appears that the skull strip step has failed as brainmask.mgz is
 completely blank. I tried to look up the error message online but found
 little help there. I would really appreciate it if someone can explain the
 problem or where to look for an explaination. I attached the recon-all error
 log with this email, i would also include nu.mgz but the last email attached
 with that was blocked due to the size of the image.
 
 Thanks!
 
 Best Regards,
 
 Cheng Peng
 
 Maryland Neuroimaging Center
 
 
 
 
 
 
 

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Re: [Freesurfer] Converting volume to label

2014-07-23 Thread Weiss, Sarah
Hi all,

We are still struggling with this issue - any ideas? Here is some more 
information:

- The surface that we are trying to label is the subject colin, as found here: 
http://mindboggle.info/data.html. We get two errors when loading in tksurfer
(1) surfer: Tailairach xform file not found (ignored)
(2) MRISreadVertexPosition(/Applications/.../subject/colin/surf/lh.org); No 
such file or directory
However, the surface does load and can be manipulated (a label can be created 
manually and filled in).

- We first tried to create a surface to overlay from our .nii file (which does 
load in Freeview) with mri_vol2surf. Our .nii had been converted to a .mgz with 
a corresponding .dat file using [COMMAND]. We tried --out_type paint (with file 
type .w) and --out_type mgh (with file type .mgh), but both gave the same error:
MRISread: file '.../surf/test-lh.w' has 0 vertices! Probably trying to use a 
scalar data file as a surface!

- We next tried mri_cor2label --i cluster1_roi.nii --l ./cluster1.label --id 1. 
In Freeview with our .nii file loaded, we verified that our volume of interest 
does have a value of 1, and unselected regions have a value of 0. It seems to 
run properly, finding unassigned vertices in label and assigning vertex numbers 
to label. However, in tksurfer, when we select File  Load label value file  
cluster1.label, we get the following error:
sclv_load_label_value_file: error reading line 1: 9389

We have two main questions - any advice would be greatly appreciated!
(1) From my understanding of label files, the first line should be the number 
of vertices in the label file. Why then do we get an error in reading this line 
when loading the label file?
(2) Are the initial errors pertaining to a lack of files for our template 
surface going to create a problem when we do successfully load our label file? 
If so, is there a way to align the two in the same coordinate space?

Thank you!

-- Sarah Weiss

From: Weiss, Sarah
Sent: Friday, July 18, 2014 3:02 PM
To: freesurfer@nmr.mgh.harvard.edu
Subject: Converting volume to label

Dear Freesurfer experts,

We are trying to convert a volume created in SPM and saved as a .nii file into 
a label to overlay on a Freesurfer surface. We are using a template surface 
found online, colin (http://mindboggle.info/data.html). We are able to display 
our .nii in freeview successfully. The voxels that we wish to label have a 
value of 1, and elsewhere in the file the value is 0. The command that we are 
running is:
mri_cor2label --i cluster3_roi.nii --l ./cluster3_roi.label --id 1
We do get a series of points as output. However, when we load the consequent 
label, it doesn't display correctly - the points are discontinuous, and don't 
correspond to the correct parts of the brain.

Here are some of the error messages that we get:
ltMNIreadEx: could not open file 
/Applications/freesurfer/subjects/rk/mri/transforms/talairach.xfm
No such file or directory
MRISreadVertexPosition(/Applications/freesurfer/subjects/rk/surf/rh.orig): 
could not open file /Applications/freesurfer/subjects/rk/surf/rh.orig
No such file or directory
MRISreadOriginalProperties: could not read surface file 
/Applications/freesurfer/subjects/rk/surf/rh.orig
No such file or directory
  0 nonzero vertices found 
label stat field identically zero - setting to 1
9389 unassigned vertices in label - building spatial LUT...
assigning vertex numbers to label...

Why does it say that 0 nonzero vertices have been found, yet it selects 9389 
vertices? Is there an additional option that we should be utilizing? Is a label 
the correct way to create what we are looking for, or would an overlay be more 
appropriate? If the latter is the case, should we be using mri_vol2surf?

Thank you in advance for your assistance!

Best,

Sarah Weiss and Robert Kim
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Re: [Freesurfer] Converting volume to label

2014-07-23 Thread Bruce Fischl

Hi Sarah

Doug is out of email contact for another week or so, and this might have 
to wait for his return. In the meantime: are you trying to overlay it on 
the colin surface or on some other subject (rk?). If you load the 
[lr]h.white surface over the volume for colin does it look ok?


cheers
Bruce

On Wed, 23 Jul 
2014, Weiss, Sarah wrote:



Hi all,
We are still struggling with this issue - any ideas? Here is some more
information:

- The surface that we are trying to label is the subject colin, as found
here: http://mindboggle.info/data.html. We get two errors when loading in
tksurfer
(1) surfer: Tailairach xform file not found (ignored)
(2) MRISreadVertexPosition(/Applications/.../subject/colin/surf/lh.org); No
such file or directory
However, the surface does load and can be manipulated (a label can be
created manually and filled in).

- We first tried to create a surface to overlay from our .nii file (which
does load in Freeview) with mri_vol2surf. Our .nii had been converted to a
.mgz with a corresponding .dat file using [COMMAND]. We tried --out_type
paint (with file type .w) and --out_type mgh (with file type .mgh), but both
gave the same error:
MRISread: file '.../surf/test-lh.w' has 0 vertices! Probably trying to use a
scalar data file as a surface!

- We next tried mri_cor2label --i cluster1_roi.nii --l ./cluster1.label --id
1. In Freeview with our .nii file loaded, we verified that our volume of
interest does have a value of 1, and unselected regions have a value of 0.
It seems to run properly, finding unassigned vertices in label and assigning
vertex numbers to label. However, in tksurfer, when we select File  Load
label value file  cluster1.label, we get the following error:
sclv_load_label_value_file: error reading line 1: 9389

We have two main questions - any advice would be greatly appreciated!
(1) From my understanding of label files, the first line should be the
number of vertices in the label file. Why then do we get an error in reading
this line when loading the label file?
(2) Are the initial errors pertaining to a lack of files for our template
surface going to create a problem when we do successfully load our label
file? If so, is there a way to align the two in the same coordinate space?

Thank you!

-- Sarah Weiss


From: Weiss, Sarah
Sent: Friday, July 18, 2014 3:02 PM
To: freesurfer@nmr.mgh.harvard.edu
Subject: Converting volume to label

Dear Freesurfer experts,
We are trying to convert a volume created in SPM and saved as a .nii file
into a label to overlay on a Freesurfer surface. We are using a template
surface found online, colin (http://mindboggle.info/data.html). We are able
to display our .nii in freeview successfully. The voxels that we wish to
label have a value of 1, and elsewhere in the file the value is 0. The
command that we are running is:
mri_cor2label --i cluster3_roi.nii --l ./cluster3_roi.label --id 1
We do get a series of points as output. However, when we load the consequent
label, it doesn't display correctly - the points are discontinuous, and
don't correspond to the correct parts of the brain.

Here are some of the error messages that we get:
ltMNIreadEx: could not open file
/Applications/freesurfer/subjects/rk/mri/transforms/talairach.xfm
No such file or directory
MRISreadVertexPosition(/Applications/freesurfer/subjects/rk/surf/rh.orig):
could not open file /Applications/freesurfer/subjects/rk/surf/rh.orig
No such file or directory
MRISreadOriginalProperties: could not read surface file
/Applications/freesurfer/subjects/rk/surf/rh.orig
No such file or directory
      0 nonzero vertices found 
label stat field identically zero - setting to 1
9389 unassigned vertices in label - building spatial LUT...
assigning vertex numbers to label...

Why does it say that 0 nonzero vertices have been found, yet it selects 9389
vertices? Is there an additional option that we should be utilizing? Is a
label the correct way to create what we are looking for, or would an overlay
be more appropriate? If the latter is the case, should we be using
mri_vol2surf?

Thank you in advance for your assistance!

Best,

Sarah Weiss and Robert Kim

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Re: [Freesurfer] lgi for longitudinal scans

2014-07-23 Thread Martin Reuter
Hi Anna,

that was not my suggestion, I suggested to pass both the cross sectional time 
point ID and the base id (together with the long flag) and additionally the 
-localGI flag.

So if you time point 1 is XXX_1 and time point 2 is XXX_2 and your base is 
XXX_base then

recon-all -long XXX_1 XXX_base -localGI

and for the second time point

recon-all -long XXX_2 XXX_base -localGI

The base is not one of your time points, it gets created in the -base step and 
you give it a name (here XXX_base). In order for the above to work you need 5 
directories per subject:

the 2 cross
XXX_1 and XXX_2
the base
XXX_base
the results of the -long runs:
XXX_1.long.XXX_base
XXX_2.long.XXX_base

the last two are the ones where the GI will be created.

Best, Martin

On Jul 23, 2014, at 9:02 AM, Anna Jonsson ajonsso...@gmail.com wrote:

 hi Martin, I just tried this and it resulted in errors/ Do I not have to 
 specify a flag for the baseid or for each subject, eg -s?
 
 What I did (according to your suggestion)
 
 recon-all -long XXX_1.long XXX_2.long -localGI
 
 This did not work? Do you know why potentially?
 
 
 On Wed, Mar 26, 2014 at 3:28 PM, Martin Reuter mreu...@nmr.mgh.harvard.edu 
 wrote:
 Hi Anna,
 
 no, you would still do:
 
 recon-all -long tpid baseid -localGI
 
 just not use the '-all'.
 
 Best, Martin
 
 
 
 
 On 03/26/2014 11:12 AM, Anna Jonsson wrote:
 thank you. But if the lnog process has already been done and these 
 induviduals already have long directories so to speak, I would just put the 
 lgi flag on as normal but just replace the subject name with the 
 long subject name eg ?
 recon-all -s _long -localGI
 
 Thank you
 
 
 On Sat, Mar 15, 2014 at 2:07 PM, Martin Reuter mreu...@nmr.mgh.harvard.edu 
 wrote:
 Hi Anna,
 
 for the image processing, you just add the lgi flag to the regular 
 longitudinal recon-all command
 recon-all -long …… -lgi
 
 for the analysis after that you use the long directories.
 
 Best, Martin
 
 On Mar 15, 2014, at 10:04 AM, Anna Jonsson ajonsso...@gmail.com wrote:
 
 Dear group,
 
 If I want conduct gyrification analyses on the long runs that have been 
 through the longitudinal stream, do I just perform the normal baseline 
 command on the .long data?
 
 thank you,
 
 kind wishes
 
 Anna
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 Freesurfer@nmr.mgh.harvard.edu
 https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
 
 -
 Dr. Martin Reuter
 Assistant in Neuroscience - Massachusetts General Hospital
 Instructor in Neurology   - Harvard Medical School
 MGH / HMS / MIT
 
 A.A.Martinos Center for Biomedical Imaging
 149 Thirteenth Street, Suite 2301
 Charlestown, MA 02129
 
 Phone: +1-617-724-5652
 Email: 
mreu...@nmr.mgh.harvard.edu
reu...@mit.edu
 Web  : http://reuter.mit.edu 
 
 
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 Freesurfer@nmr.mgh.harvard.edu
 https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
 
 
 The information in this e-mail is intended only for the person to whom it is
 addressed. If you believe this e-mail was sent to you in error and the e-mail
 contains patient information, please contact the Partners Compliance 
 HelpLine at
 http://www.partners.org/complianceline . If the e-mail was sent to you in 
 error
 but does not contain patient information, please contact the sender and 
 properly
 dispose of the e-mail.
 
 
 
 -- 
 Martin Reuter, Ph.D.
 
 Instructor in Neurology
   Harvard Medical School
 Assistant in Neuroscience
   Dept. of Radiology, Massachusetts General Hospital
   Dept. of Neurology, Massachusetts General Hospital
 Research Affiliate
   Computer Science and Artificial Intelligence Lab,
   Dept. of Electrical Engineering and Computer Science,
   Massachusetts Institute of Technology
 
 A.A.Martinos Center for Biomedical Imaging
 149 Thirteenth Street, Suite 2301
 Charlestown, MA 02129
 
 Phone: +1-617-724-5652
 
 Email: 
mreu...@nmr.mgh.harvard.edu
reu...@mit.edu
 Web  : http://reuter.mit.edu 
 

-
Dr. Martin Reuter
Assistant in Neuroscience - Massachusetts General Hospital
Instructor in Neurology   - Harvard Medical School
MGH / HMS / MIT

A.A.Martinos Center for Biomedical Imaging
149 Thirteenth Street, Suite 2301
Charlestown, MA 02129

Phone: +1-617-724-5652
Email: 
   mreu...@nmr.mgh.harvard.edu
   reu...@mit.edu
Web  : http://reuter.mit.edu 

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Re: [Freesurfer] recon-all error

2014-07-23 Thread Cheng Peng
Hi, Bruce:

I attached the file with an email and sent it to you an hour ago and received 
an automatic reply saying it's pending for approval due to the size of the 
email. Is there another way to upload my image or it's getting processed? I 
don't want to spam you guys since i already sent it twice and got the same 
result.

Thanks!

Cheng Peng


On Wednesday, July 23, 2014 9:39 AM, Cheng Peng pcdula...@yahoo.com wrote:
 


Hi, Bruce,

I attached the nu.mgz file with this email, i'm at home right now and would 
upload T1.mgz later if need to. As far as i know individual recon-all runs were 
completed without error (two scans on one subject, two hours apart), really 
appreciate the help!

Cheng Peng

Maryland Neuroimaging Center


On Wednesday, July 23, 2014 9:32 AM, Bruce Fischl fis...@nmr.mgh.harvard.edu 
wrote:
 


Hi Cheng

do the cross-sectional runs look okay? The base? If so, does the first 
intensity normalized images look ok (the T1.mgz and the nu.mgz)? If you 
can't track it down, upload the subject and we will take a look

cheers
Bruce


On Wed, 23 
Jul 2014, Cheng Peng wrote:

 Hi, Freesurfer experts:
 
 
 
 I encountered an error message while i was attempting to run a longitudinal
 study (following the instructions from
 https://surfer.nmr.mgh.harvard.edu/fswiki/LongitudinalProcessing, step 2
 under workflow summary)
 
 egister_mri: find_optimal_transform
 find_optimal_transform: nsamples 2185, passno 0, spacing 8
 GCAhistoScaleImageIntensities: could not find wm peak
 resetting wm mean[0]: 102 -- 107
 resetting gm mean[0]: 64 -- 64
 input volume #1 is the most T1-like
 using real data threshold=0.0
 skull bounding box =
 (0, 0, 0) -- (255, 255, 255)
 using (85, 85, 128) as brain centroid...
 mean wm in atlas = 107, using box (53,53,96) -- (116, 116,159) to find MRI
 wm
 WARNING: gca.c::GCAhistoScaleImageIntensities: h_mri-nbins=1, mri_peak=-1
 before smoothing, mri peak at 0
 WARNING2: gca.c::GCAhistoScaleImageIntensities: h_mri-nbins=1, mri_peak=-1
 after smoothing, mri peak at 0, scaling input intensities by inf
 
 It appears that the skull strip step has failed as brainmask.mgz is
 completely blank. I tried to look up the error message online but found
 little help there. I would really appreciate it if someone can explain the
 problem or where to look for an explaination. I attached the recon-all error
 log with this email, i would also include nu.mgz but the last email attached
 with that was blocked due to the size of the image.
 
 Thanks!
 
 Best Regards,
 
 Cheng Peng
 
 Maryland Neuroimaging Center
 
 
 
 
 
 
 

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Re: [Freesurfer] recon-all error

2014-07-23 Thread Bruce Fischl
Hi Cheng

if you go to our wiki there are instructions for uploading big datasets 
using either ftp or our filedrop

cheers
Bruce
On Wed, 23 Jul 2014, Cheng Peng wrote:

 Hi, Bruce:
 
 I attached the file with an email and sent it to you an hour ago and
 received an automatic reply saying it's pending for approval due to the size
 of the email. Is there another way to upload my image or it's getting
 processed? I don't want to spam you guys since i already sent it twice and
 got the same result.
 
 Thanks!
 
 Cheng Peng
 
 
 On Wednesday, July 23, 2014 9:39 AM, Cheng Peng pcdula...@yahoo.com wrote:
 
 
 Hi, Bruce,
 
 I attached the nu.mgz file with this email, i'm at home right now and would
 upload T1.mgz later if need to. As far as i know individual recon-all runs
 were completed without error (two scans on one subject, two hours apart),
 really appreciate the help!
 
 Cheng Peng
 
 Maryland Neuroimaging Center
 
 
 On Wednesday, July 23, 2014 9:32 AM, Bruce Fischl
 fis...@nmr.mgh.harvard.edu wrote:
 
 
 Hi Cheng
 
 do the cross-sectional runs look okay? The base? If so, does the first
 intensity normalized images look ok (the T1.mgz and the nu.mgz)? If you
 can't track it down, upload the subject and we will take a look
 
 cheers
 Bruce
 
 On Wed, 23
 Jul 2014, Cheng Peng wrote:
 
  Hi, Freesurfer experts:
 
 
 
  I encountered an error message while i was attempting to run a
 longitudinal
  study (following the instructions from
  https://surfer.nmr.mgh.harvard.edu/fswiki/LongitudinalProcessing, step 2
  under workflow summary)
 
  egister_mri: find_optimal_transform
  find_optimal_transform: nsamples 2185, passno 0, spacing 8
  GCAhistoScaleImageIntensities: could not find wm peak
  resetting wm mean[0]: 102 -- 107
  resetting gm mean[0]: 64 -- 64
  input volume #1 is the most T1-like
  using real data threshold=0.0
  skull bounding box = (0, 0, 0) -- (255, 255, 255)
  using (85, 85, 128) as brain centroid...
  mean wm in atlas = 107, using box (53,53,96) -- (116, 116,159) to find
 MRI
  wm
  WARNING: gca.c::GCAhistoScaleImageIntensities: h_mri-nbins=1, mri_peak=-1
  before smoothing, mri peak at 0
  WARNING2: gca.c::GCAhistoScaleImageIntensities: h_mri-nbins=1,
 mri_peak=-1
  after smoothing, mri peak at 0, scaling input intensities by inf
 
  It appears that the skull strip step has failed as brainmask.mgz is
  completely blank. I tried to look up the error message online but found
  little help there. I would really appreciate it if someone can explain the
  problem or where to look for an explaination. I attached the recon-all
 error
  log with this email, i would also include nu.mgz but the last email
 attached
  with that was blocked due to the size of the image.
 
  Thanks!
 
  Best Regards,
 
  Cheng Peng
 
  Maryland Neuroimaging Center
 
 
 
 
 
 
 
 
 
 ___
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 Freesurfer@nmr.mgh.harvard.edu
 https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
 
 
 The information in this e-mail is intended only for the person to whom it is
 addressed. If you believe this e-mail was sent to you in error and the
 e-mail
 contains patient information, please contact the Partners Compliance
 HelpLine at
 http://www.partners.org/complianceline . If the e-mail was sent to you in
 error
 but does not contain patient information, please contact the sender and
 properly
 dispose of the e-mail.
 
 
 
 
 
 

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[Freesurfer] qcache number of vertices error

2014-07-23 Thread O'Shea,Andrew
Hello,
I am running into an error when using qcache. Subjects that have had manual 
edits are exited with errors, while subjects that have not had edits run fine. 
All subjects have had –make all ran after edits and finished without errors. I 
have read about this problem and tried some of the solutions but am still 
getting errors.

When I first tried running –qcache I received this error:

Reading curvature file 
/scratch/lfs/aoshea/testAndrew/processedSubjects/1492/surf/lh.volume

ERROR: number of vertices in 
/scratch/lfs/aoshea/testAndrew/processedSubjects/1492/surf/lh.volume does not 
match surface (128222,130849)

ERROR: reading curvature file

Linux c2a-s21.ufhpc 2.6.32-358.23.2.el6.x86_64 #1 SMP Sat Sep 14 05:32:37 EDT 
2013 x86_64 x86_64 x86_64 GNU/Linux


recon-all -s 1492 exited with ERRORS at Wed Jul 23 10:32:24 EDT 2014


To make sure everything was ran I re-ran –make all and got make: Nothing to be 
done for `all’.


I then ran –vno_match_check and it said everything was fine:

Pass: all surfaces and surface data for subject 1492 for hemi lh have the same 
number of vertices.

Pass: all surfaces and surface data for subject 1492 for hemi rh have the same 
number of vertices.

I saw Doug gave this suggestion ( using –surf volume –qcache) to someone who 
was having the same problem this resulted in qcache getting past the volume 
stage but failing lh.w-g.pct.mgh

Reading curvature file 
/scratch/lfs/aoshea/testAndrew/processedSubjects/1492/surf/lh.w-g.pct.mgh

ERROR: number of vertices in 
/scratch/lfs/aoshea/testAndrew/processedSubjects/1492/surf/lh.w-g.pct.mgh does 
not match surface (128222,130849)

ERROR: reading curvature file

Linux c1a-s11.ufhpc 2.6.32-358.23.2.el6.x86_64 #1 SMP Sat Sep 14 05:32:37 EDT 
2013 x86_64 x86_64 x86_64 GNU/Linux


recon-all -s 1492 exited with ERRORS at Wed Jul 23 11:07:01 EDT 2014


Any suggestions on how to address this? Any idea of the cause? I assuming it 
has something to do with the manual edits since unedited ppl run fine.

-Andrew
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Re: [Freesurfer] Converting volume to label

2014-07-23 Thread Weiss, Sarah
Hi Bruce,

Thanks for the input. Here are some of the output lines that I get when I try 
overlaying one of our ROI surfaces (test-eh.mgh) onto colin (we had created a 
copy rk, but are back to using the original as downloaded):
tksurfer colin rh white -overlay /Users/../test-rh.mgh
sufer: Tailrach xform file not found (ignored) -- this is as before
tkmedit: Trying to open /Users/.../test-rh.dat
surfer: Interpreting overlay volume /Users/.../test-rh.mgh as encoded scalar 
volume.
MRISreadCurvature: could not open 
/Applications/freesurfer/.../colin/surf/rh.curv
surfer: error reading curvature file
surfer: tkoInitWindow(colin)
reading white matter vertex locations

When I place the mouse over the displayed colin surface in tksurfer, although 
the displayed option at the bottom is test-rh.mgh, the resultant value is ** 
0.00 **.

Do I need to create a test-rh.dat file? How would I go about doing that? 
Should my template brain colin have a curvature file?

Again, thank you for any inputs or suggestions!

-- Sarah

From: freesurfer-boun...@nmr.mgh.harvard.edu 
[freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
[fis...@nmr.mgh.harvard.edu]
Sent: Wednesday, July 23, 2014 9:52 AM
To: Freesurfer support list
Subject: Re: [Freesurfer] Converting volume to label

Hi Sarah

Doug is out of email contact for another week or so, and this might have
to wait for his return. In the meantime: are you trying to overlay it on
the colin surface or on some other subject (rk?). If you load the
[lr]h.white surface over the volume for colin does it look ok?

cheers
Bruce

On Wed, 23 Jul
2014, Weiss, Sarah wrote:

 Hi all,
 We are still struggling with this issue - any ideas? Here is some more
 information:

 - The surface that we are trying to label is the subject colin, as found
 here: http://mindboggle.info/data.html. We get two errors when loading in
 tksurfer
 (1) surfer: Tailairach xform file not found (ignored)
 (2) MRISreadVertexPosition(/Applications/.../subject/colin/surf/lh.org); No
 such file or directory
 However, the surface does load and can be manipulated (a label can be
 created manually and filled in).

 - We first tried to create a surface to overlay from our .nii file (which
 does load in Freeview) with mri_vol2surf. Our .nii had been converted to a
 .mgz with a corresponding .dat file using [COMMAND]. We tried --out_type
 paint (with file type .w) and --out_type mgh (with file type .mgh), but both
 gave the same error:
 MRISread: file '.../surf/test-lh.w' has 0 vertices! Probably trying to use a
 scalar data file as a surface!

 - We next tried mri_cor2label --i cluster1_roi.nii --l ./cluster1.label --id
 1. In Freeview with our .nii file loaded, we verified that our volume of
 interest does have a value of 1, and unselected regions have a value of 0.
 It seems to run properly, finding unassigned vertices in label and assigning
 vertex numbers to label. However, in tksurfer, when we select File  Load
 label value file  cluster1.label, we get the following error:
 sclv_load_label_value_file: error reading line 1: 9389

 We have two main questions - any advice would be greatly appreciated!
 (1) From my understanding of label files, the first line should be the
 number of vertices in the label file. Why then do we get an error in reading
 this line when loading the label file?
 (2) Are the initial errors pertaining to a lack of files for our template
 surface going to create a problem when we do successfully load our label
 file? If so, is there a way to align the two in the same coordinate space?

 Thank you!

 -- Sarah Weiss

 
 From: Weiss, Sarah
 Sent: Friday, July 18, 2014 3:02 PM
 To: freesurfer@nmr.mgh.harvard.edu
 Subject: Converting volume to label

 Dear Freesurfer experts,
 We are trying to convert a volume created in SPM and saved as a .nii file
 into a label to overlay on a Freesurfer surface. We are using a template
 surface found online, colin (http://mindboggle.info/data.html). We are able
 to display our .nii in freeview successfully. The voxels that we wish to
 label have a value of 1, and elsewhere in the file the value is 0. The
 command that we are running is:
 mri_cor2label --i cluster3_roi.nii --l ./cluster3_roi.label --id 1
 We do get a series of points as output. However, when we load the consequent
 label, it doesn't display correctly - the points are discontinuous, and
 don't correspond to the correct parts of the brain.

 Here are some of the error messages that we get:
 ltMNIreadEx: could not open file
 /Applications/freesurfer/subjects/rk/mri/transforms/talairach.xfm
 No such file or directory
 MRISreadVertexPosition(/Applications/freesurfer/subjects/rk/surf/rh.orig):
 could not open file /Applications/freesurfer/subjects/rk/surf/rh.orig
 No such file or directory
 MRISreadOriginalProperties: could not read surface file
 

Re: [Freesurfer] Converting volume to label

2014-07-23 Thread Bruce Fischl
Hi Sarah

how did you create the test-rh.mgh? If you bring it up as a volume in 
freeview over one of the colin volumes, do they align? Do the 
lh.white/rh.white also align with your colin volume?

Bruce

p.s. can you remind me where you got the FS-processed colin data?


On Wed, 23 Jul 2014, Weiss, Sarah wrote:

 Hi Bruce,

 Thanks for the input. Here are some of the output lines that I get when I try 
 overlaying one of our ROI surfaces (test-eh.mgh) onto colin (we had created a 
 copy rk, but are back to using the original as downloaded):
 tksurfer colin rh white -overlay /Users/../test-rh.mgh
 sufer: Tailrach xform file not found (ignored) -- this is as before
 tkmedit: Trying to open /Users/.../test-rh.dat
 surfer: Interpreting overlay volume /Users/.../test-rh.mgh as encoded scalar 
 volume.
 MRISreadCurvature: could not open 
 /Applications/freesurfer/.../colin/surf/rh.curv
 surfer: error reading curvature file
 surfer: tkoInitWindow(colin)
 reading white matter vertex locations

 When I place the mouse over the displayed colin surface in tksurfer, although 
 the displayed option at the bottom is test-rh.mgh, the resultant value is 
 ** 0.00 **.

 Do I need to create a test-rh.dat file? How would I go about doing that? 
 Should my template brain colin have a curvature file?

 Again, thank you for any inputs or suggestions!

 -- Sarah
 
 From: freesurfer-boun...@nmr.mgh.harvard.edu 
 [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
 [fis...@nmr.mgh.harvard.edu]
 Sent: Wednesday, July 23, 2014 9:52 AM
 To: Freesurfer support list
 Subject: Re: [Freesurfer] Converting volume to label

 Hi Sarah

 Doug is out of email contact for another week or so, and this might have
 to wait for his return. In the meantime: are you trying to overlay it on
 the colin surface or on some other subject (rk?). If you load the
 [lr]h.white surface over the volume for colin does it look ok?

 cheers
 Bruce

 On Wed, 23 Jul
 2014, Weiss, Sarah wrote:

 Hi all,
 We are still struggling with this issue - any ideas? Here is some more
 information:

 - The surface that we are trying to label is the subject colin, as found
 here: http://mindboggle.info/data.html. We get two errors when loading in
 tksurfer
 (1) surfer: Tailairach xform file not found (ignored)
 (2) MRISreadVertexPosition(/Applications/.../subject/colin/surf/lh.org); No
 such file or directory
 However, the surface does load and can be manipulated (a label can be
 created manually and filled in).

 - We first tried to create a surface to overlay from our .nii file (which
 does load in Freeview) with mri_vol2surf. Our .nii had been converted to a
 .mgz with a corresponding .dat file using [COMMAND]. We tried --out_type
 paint (with file type .w) and --out_type mgh (with file type .mgh), but both
 gave the same error:
 MRISread: file '.../surf/test-lh.w' has 0 vertices! Probably trying to use a
 scalar data file as a surface!

 - We next tried mri_cor2label --i cluster1_roi.nii --l ./cluster1.label --id
 1. In Freeview with our .nii file loaded, we verified that our volume of
 interest does have a value of 1, and unselected regions have a value of 0.
 It seems to run properly, finding unassigned vertices in label and assigning
 vertex numbers to label. However, in tksurfer, when we select File  Load
 label value file  cluster1.label, we get the following error:
 sclv_load_label_value_file: error reading line 1: 9389

 We have two main questions - any advice would be greatly appreciated!
 (1) From my understanding of label files, the first line should be the
 number of vertices in the label file. Why then do we get an error in reading
 this line when loading the label file?
 (2) Are the initial errors pertaining to a lack of files for our template
 surface going to create a problem when we do successfully load our label
 file? If so, is there a way to align the two in the same coordinate space?

 Thank you!

 -- Sarah Weiss

 
 From: Weiss, Sarah
 Sent: Friday, July 18, 2014 3:02 PM
 To: freesurfer@nmr.mgh.harvard.edu
 Subject: Converting volume to label

 Dear Freesurfer experts,
 We are trying to convert a volume created in SPM and saved as a .nii file
 into a label to overlay on a Freesurfer surface. We are using a template
 surface found online, colin (http://mindboggle.info/data.html). We are able
 to display our .nii in freeview successfully. The voxels that we wish to
 label have a value of 1, and elsewhere in the file the value is 0. The
 command that we are running is:
 mri_cor2label --i cluster3_roi.nii --l ./cluster3_roi.label --id 1
 We do get a series of points as output. However, when we load the consequent
 label, it doesn't display correctly - the points are discontinuous, and
 don't correspond to the correct parts of the brain.

 Here are some of the error messages that we get:
 ltMNIreadEx: could not open file
 

Re: [Freesurfer] Converting volume to label

2014-07-23 Thread Weiss, Sarah
Hi Bruce,

To create the .mgh, we used the following command:
mri_vol2surf --src cluster1_roi.mgz --srcreg cluster1_roi.dat --hemi rh --o 
./test-rh.mgh --out_type mgh --float2int tkregister

I can view both the .nii and the .mgz in Freeview but the /test-rh.mgh does not 
appear to have any nonzero vertices. Any thoughts on why this might be?

From: freesurfer-boun...@nmr.mgh.harvard.edu 
[freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
[fis...@nmr.mgh.harvard.edu]
Sent: Wednesday, July 23, 2014 12:22 PM
To: Freesurfer support list
Subject: Re: [Freesurfer] Converting volume to label

Hi Sarah

how did you create the test-rh.mgh? If you bring it up as a volume in
freeview over one of the colin volumes, do they align? Do the
lh.white/rh.white also align with your colin volume?

Bruce

p.s. can you remind me where you got the FS-processed colin data?


On Wed, 23 Jul 2014, Weiss, Sarah wrote:

 Hi Bruce,

 Thanks for the input. Here are some of the output lines that I get when I try 
 overlaying one of our ROI surfaces (test-eh.mgh) onto colin (we had created a 
 copy rk, but are back to using the original as downloaded):
 tksurfer colin rh white -overlay /Users/../test-rh.mgh
 sufer: Tailrach xform file not found (ignored) -- this is as before
 tkmedit: Trying to open /Users/.../test-rh.dat
 surfer: Interpreting overlay volume /Users/.../test-rh.mgh as encoded scalar 
 volume.
 MRISreadCurvature: could not open 
 /Applications/freesurfer/.../colin/surf/rh.curv
 surfer: error reading curvature file
 surfer: tkoInitWindow(colin)
 reading white matter vertex locations

 When I place the mouse over the displayed colin surface in tksurfer, although 
 the displayed option at the bottom is test-rh.mgh, the resultant value is 
 ** 0.00 **.

 Do I need to create a test-rh.dat file? How would I go about doing that? 
 Should my template brain colin have a curvature file?

 Again, thank you for any inputs or suggestions!

 -- Sarah
 
 From: freesurfer-boun...@nmr.mgh.harvard.edu 
 [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
 [fis...@nmr.mgh.harvard.edu]
 Sent: Wednesday, July 23, 2014 9:52 AM
 To: Freesurfer support list
 Subject: Re: [Freesurfer] Converting volume to label

 Hi Sarah

 Doug is out of email contact for another week or so, and this might have
 to wait for his return. In the meantime: are you trying to overlay it on
 the colin surface or on some other subject (rk?). If you load the
 [lr]h.white surface over the volume for colin does it look ok?

 cheers
 Bruce

 On Wed, 23 Jul
 2014, Weiss, Sarah wrote:

 Hi all,
 We are still struggling with this issue - any ideas? Here is some more
 information:

 - The surface that we are trying to label is the subject colin, as found
 here: http://mindboggle.info/data.html. We get two errors when loading in
 tksurfer
 (1) surfer: Tailairach xform file not found (ignored)
 (2) MRISreadVertexPosition(/Applications/.../subject/colin/surf/lh.org); No
 such file or directory
 However, the surface does load and can be manipulated (a label can be
 created manually and filled in).

 - We first tried to create a surface to overlay from our .nii file (which
 does load in Freeview) with mri_vol2surf. Our .nii had been converted to a
 .mgz with a corresponding .dat file using [COMMAND]. We tried --out_type
 paint (with file type .w) and --out_type mgh (with file type .mgh), but both
 gave the same error:
 MRISread: file '.../surf/test-lh.w' has 0 vertices! Probably trying to use a
 scalar data file as a surface!

 - We next tried mri_cor2label --i cluster1_roi.nii --l ./cluster1.label --id
 1. In Freeview with our .nii file loaded, we verified that our volume of
 interest does have a value of 1, and unselected regions have a value of 0.
 It seems to run properly, finding unassigned vertices in label and assigning
 vertex numbers to label. However, in tksurfer, when we select File  Load
 label value file  cluster1.label, we get the following error:
 sclv_load_label_value_file: error reading line 1: 9389

 We have two main questions - any advice would be greatly appreciated!
 (1) From my understanding of label files, the first line should be the
 number of vertices in the label file. Why then do we get an error in reading
 this line when loading the label file?
 (2) Are the initial errors pertaining to a lack of files for our template
 surface going to create a problem when we do successfully load our label
 file? If so, is there a way to align the two in the same coordinate space?

 Thank you!

 -- Sarah Weiss

 
 From: Weiss, Sarah
 Sent: Friday, July 18, 2014 3:02 PM
 To: freesurfer@nmr.mgh.harvard.edu
 Subject: Converting volume to label

 Dear Freesurfer experts,
 We are trying to convert a volume created in SPM and saved as a .nii file
 into a label to overlay on a 

Re: [Freesurfer] Converting volume to label

2014-07-23 Thread Bruce Fischl
Hi Sarah

how did you create cluster1_roi.mgz? Is it in register with colin?
Bruce
On Wed, 
23 Jul 2014, Weiss, Sarah wrote:

 Hi Bruce,

 To create the .mgh, we used the following command:
 mri_vol2surf --src cluster1_roi.mgz --srcreg cluster1_roi.dat --hemi rh --o 
 ./test-rh.mgh --out_type mgh --float2int tkregister

 I can view both the .nii and the .mgz in Freeview but the /test-rh.mgh does 
 not appear to have any nonzero vertices. Any thoughts on why this might be?
 
 From: freesurfer-boun...@nmr.mgh.harvard.edu 
 [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
 [fis...@nmr.mgh.harvard.edu]
 Sent: Wednesday, July 23, 2014 12:22 PM
 To: Freesurfer support list
 Subject: Re: [Freesurfer] Converting volume to label

 Hi Sarah

 how did you create the test-rh.mgh? If you bring it up as a volume in
 freeview over one of the colin volumes, do they align? Do the
 lh.white/rh.white also align with your colin volume?

 Bruce

 p.s. can you remind me where you got the FS-processed colin data?


 On Wed, 23 Jul 2014, Weiss, Sarah wrote:

 Hi Bruce,

 Thanks for the input. Here are some of the output lines that I get when I 
 try overlaying one of our ROI surfaces (test-eh.mgh) onto colin (we had 
 created a copy rk, but are back to using the original as downloaded):
 tksurfer colin rh white -overlay /Users/../test-rh.mgh
 sufer: Tailrach xform file not found (ignored) -- this is as before
 tkmedit: Trying to open /Users/.../test-rh.dat
 surfer: Interpreting overlay volume /Users/.../test-rh.mgh as encoded scalar 
 volume.
 MRISreadCurvature: could not open 
 /Applications/freesurfer/.../colin/surf/rh.curv
 surfer: error reading curvature file
 surfer: tkoInitWindow(colin)
 reading white matter vertex locations

 When I place the mouse over the displayed colin surface in tksurfer, 
 although the displayed option at the bottom is test-rh.mgh, the resultant 
 value is ** 0.00 **.

 Do I need to create a test-rh.dat file? How would I go about doing that? 
 Should my template brain colin have a curvature file?

 Again, thank you for any inputs or suggestions!

 -- Sarah
 
 From: freesurfer-boun...@nmr.mgh.harvard.edu 
 [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
 [fis...@nmr.mgh.harvard.edu]
 Sent: Wednesday, July 23, 2014 9:52 AM
 To: Freesurfer support list
 Subject: Re: [Freesurfer] Converting volume to label

 Hi Sarah

 Doug is out of email contact for another week or so, and this might have
 to wait for his return. In the meantime: are you trying to overlay it on
 the colin surface or on some other subject (rk?). If you load the
 [lr]h.white surface over the volume for colin does it look ok?

 cheers
 Bruce

 On Wed, 23 Jul
 2014, Weiss, Sarah wrote:

 Hi all,
 We are still struggling with this issue - any ideas? Here is some more
 information:

 - The surface that we are trying to label is the subject colin, as found
 here: http://mindboggle.info/data.html. We get two errors when loading in
 tksurfer
 (1) surfer: Tailairach xform file not found (ignored)
 (2) MRISreadVertexPosition(/Applications/.../subject/colin/surf/lh.org); No
 such file or directory
 However, the surface does load and can be manipulated (a label can be
 created manually and filled in).

 - We first tried to create a surface to overlay from our .nii file (which
 does load in Freeview) with mri_vol2surf. Our .nii had been converted to a
 .mgz with a corresponding .dat file using [COMMAND]. We tried --out_type
 paint (with file type .w) and --out_type mgh (with file type .mgh), but both
 gave the same error:
 MRISread: file '.../surf/test-lh.w' has 0 vertices! Probably trying to use a
 scalar data file as a surface!

 - We next tried mri_cor2label --i cluster1_roi.nii --l ./cluster1.label --id
 1. In Freeview with our .nii file loaded, we verified that our volume of
 interest does have a value of 1, and unselected regions have a value of 0.
 It seems to run properly, finding unassigned vertices in label and assigning
 vertex numbers to label. However, in tksurfer, when we select File  Load
 label value file  cluster1.label, we get the following error:
 sclv_load_label_value_file: error reading line 1: 9389

 We have two main questions - any advice would be greatly appreciated!
 (1) From my understanding of label files, the first line should be the
 number of vertices in the label file. Why then do we get an error in reading
 this line when loading the label file?
 (2) Are the initial errors pertaining to a lack of files for our template
 surface going to create a problem when we do successfully load our label
 file? If so, is there a way to align the two in the same coordinate space?

 Thank you!

 -- Sarah Weiss

 
 From: Weiss, Sarah
 Sent: Friday, July 18, 2014 3:02 PM
 To: freesurfer@nmr.mgh.harvard.edu
 Subject: Converting volume to 

[Freesurfer] wm/pial surfaces from manually created surfaces

2014-07-23 Thread Itamar Kahn
Greetings,

I would like to use two surfaces that I created manually on the same anatomy 
and to create a single subject with the surfaces such that one is the pial and 
the other is the wm surface.
Any leads on how to achieve this will be greatly appreciated.

Thanks,
Itamar


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Re: [Freesurfer] Converting volume to label

2014-07-23 Thread Weiss, Sarah
Hi Bruce,

I believe that we used the command recon-all to create cluster1_roi.mgz. When 
opened in freeview along with colin, it appears to be in alignment. I'm not 
clear on where our cluster1_roi.dat file originated from though - I'll look 
into that.

From: freesurfer-boun...@nmr.mgh.harvard.edu 
[freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
[fis...@nmr.mgh.harvard.edu]
Sent: Wednesday, July 23, 2014 1:23 PM
To: Freesurfer support list
Subject: Re: [Freesurfer] Converting volume to label

Hi Sarah

how did you create cluster1_roi.mgz? Is it in register with colin?
Bruce
On Wed,
23 Jul 2014, Weiss, Sarah wrote:

 Hi Bruce,

 To create the .mgh, we used the following command:
 mri_vol2surf --src cluster1_roi.mgz --srcreg cluster1_roi.dat --hemi rh --o 
 ./test-rh.mgh --out_type mgh --float2int tkregister

 I can view both the .nii and the .mgz in Freeview but the /test-rh.mgh does 
 not appear to have any nonzero vertices. Any thoughts on why this might be?
 
 From: freesurfer-boun...@nmr.mgh.harvard.edu 
 [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
 [fis...@nmr.mgh.harvard.edu]
 Sent: Wednesday, July 23, 2014 12:22 PM
 To: Freesurfer support list
 Subject: Re: [Freesurfer] Converting volume to label

 Hi Sarah

 how did you create the test-rh.mgh? If you bring it up as a volume in
 freeview over one of the colin volumes, do they align? Do the
 lh.white/rh.white also align with your colin volume?

 Bruce

 p.s. can you remind me where you got the FS-processed colin data?


 On Wed, 23 Jul 2014, Weiss, Sarah wrote:

 Hi Bruce,

 Thanks for the input. Here are some of the output lines that I get when I 
 try overlaying one of our ROI surfaces (test-eh.mgh) onto colin (we had 
 created a copy rk, but are back to using the original as downloaded):
 tksurfer colin rh white -overlay /Users/../test-rh.mgh
 sufer: Tailrach xform file not found (ignored) -- this is as before
 tkmedit: Trying to open /Users/.../test-rh.dat
 surfer: Interpreting overlay volume /Users/.../test-rh.mgh as encoded scalar 
 volume.
 MRISreadCurvature: could not open 
 /Applications/freesurfer/.../colin/surf/rh.curv
 surfer: error reading curvature file
 surfer: tkoInitWindow(colin)
 reading white matter vertex locations

 When I place the mouse over the displayed colin surface in tksurfer, 
 although the displayed option at the bottom is test-rh.mgh, the resultant 
 value is ** 0.00 **.

 Do I need to create a test-rh.dat file? How would I go about doing that? 
 Should my template brain colin have a curvature file?

 Again, thank you for any inputs or suggestions!

 -- Sarah
 
 From: freesurfer-boun...@nmr.mgh.harvard.edu 
 [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
 [fis...@nmr.mgh.harvard.edu]
 Sent: Wednesday, July 23, 2014 9:52 AM
 To: Freesurfer support list
 Subject: Re: [Freesurfer] Converting volume to label

 Hi Sarah

 Doug is out of email contact for another week or so, and this might have
 to wait for his return. In the meantime: are you trying to overlay it on
 the colin surface or on some other subject (rk?). If you load the
 [lr]h.white surface over the volume for colin does it look ok?

 cheers
 Bruce

 On Wed, 23 Jul
 2014, Weiss, Sarah wrote:

 Hi all,
 We are still struggling with this issue - any ideas? Here is some more
 information:

 - The surface that we are trying to label is the subject colin, as found
 here: http://mindboggle.info/data.html. We get two errors when loading in
 tksurfer
 (1) surfer: Tailairach xform file not found (ignored)
 (2) MRISreadVertexPosition(/Applications/.../subject/colin/surf/lh.org); No
 such file or directory
 However, the surface does load and can be manipulated (a label can be
 created manually and filled in).

 - We first tried to create a surface to overlay from our .nii file (which
 does load in Freeview) with mri_vol2surf. Our .nii had been converted to a
 .mgz with a corresponding .dat file using [COMMAND]. We tried --out_type
 paint (with file type .w) and --out_type mgh (with file type .mgh), but both
 gave the same error:
 MRISread: file '.../surf/test-lh.w' has 0 vertices! Probably trying to use a
 scalar data file as a surface!

 - We next tried mri_cor2label --i cluster1_roi.nii --l ./cluster1.label --id
 1. In Freeview with our .nii file loaded, we verified that our volume of
 interest does have a value of 1, and unselected regions have a value of 0.
 It seems to run properly, finding unassigned vertices in label and assigning
 vertex numbers to label. However, in tksurfer, when we select File  Load
 label value file  cluster1.label, we get the following error:
 sclv_load_label_value_file: error reading line 1: 9389

 We have two main questions - any advice would be greatly appreciated!
 (1) From my understanding of label files, the first line should be the
 number of 

Re: [Freesurfer] Converting volume to label

2014-07-23 Thread Bruce Fischl
if it's in alignment without the .dat file you probably shouldn't use the 
dat file in the sampling onto the surface. I don't think it was created 
by recon-all though

cheers
Bruce
On Wed, 23 Jul 2014, Weiss, Sarah wrote:

 Hi Bruce,

 I believe that we used the command recon-all to create cluster1_roi.mgz. When 
 opened in freeview along with colin, it appears to be in alignment. I'm not 
 clear on where our cluster1_roi.dat file originated from though - I'll look 
 into that.
 
 From: freesurfer-boun...@nmr.mgh.harvard.edu 
 [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
 [fis...@nmr.mgh.harvard.edu]
 Sent: Wednesday, July 23, 2014 1:23 PM
 To: Freesurfer support list
 Subject: Re: [Freesurfer] Converting volume to label

 Hi Sarah

 how did you create cluster1_roi.mgz? Is it in register with colin?
 Bruce
 On Wed,
 23 Jul 2014, Weiss, Sarah wrote:

 Hi Bruce,

 To create the .mgh, we used the following command:
 mri_vol2surf --src cluster1_roi.mgz --srcreg cluster1_roi.dat --hemi rh --o 
 ./test-rh.mgh --out_type mgh --float2int tkregister

 I can view both the .nii and the .mgz in Freeview but the /test-rh.mgh does 
 not appear to have any nonzero vertices. Any thoughts on why this might be?
 
 From: freesurfer-boun...@nmr.mgh.harvard.edu 
 [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
 [fis...@nmr.mgh.harvard.edu]
 Sent: Wednesday, July 23, 2014 12:22 PM
 To: Freesurfer support list
 Subject: Re: [Freesurfer] Converting volume to label

 Hi Sarah

 how did you create the test-rh.mgh? If you bring it up as a volume in
 freeview over one of the colin volumes, do they align? Do the
 lh.white/rh.white also align with your colin volume?

 Bruce

 p.s. can you remind me where you got the FS-processed colin data?


 On Wed, 23 Jul 2014, Weiss, Sarah wrote:

 Hi Bruce,

 Thanks for the input. Here are some of the output lines that I get when I 
 try overlaying one of our ROI surfaces (test-eh.mgh) onto colin (we had 
 created a copy rk, but are back to using the original as downloaded):
 tksurfer colin rh white -overlay /Users/../test-rh.mgh
 sufer: Tailrach xform file not found (ignored) -- this is as before
 tkmedit: Trying to open /Users/.../test-rh.dat
 surfer: Interpreting overlay volume /Users/.../test-rh.mgh as encoded 
 scalar volume.
 MRISreadCurvature: could not open 
 /Applications/freesurfer/.../colin/surf/rh.curv
 surfer: error reading curvature file
 surfer: tkoInitWindow(colin)
 reading white matter vertex locations

 When I place the mouse over the displayed colin surface in tksurfer, 
 although the displayed option at the bottom is test-rh.mgh, the resultant 
 value is ** 0.00 **.

 Do I need to create a test-rh.dat file? How would I go about doing that? 
 Should my template brain colin have a curvature file?

 Again, thank you for any inputs or suggestions!

 -- Sarah
 
 From: freesurfer-boun...@nmr.mgh.harvard.edu 
 [freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Bruce Fischl 
 [fis...@nmr.mgh.harvard.edu]
 Sent: Wednesday, July 23, 2014 9:52 AM
 To: Freesurfer support list
 Subject: Re: [Freesurfer] Converting volume to label

 Hi Sarah

 Doug is out of email contact for another week or so, and this might have
 to wait for his return. In the meantime: are you trying to overlay it on
 the colin surface or on some other subject (rk?). If you load the
 [lr]h.white surface over the volume for colin does it look ok?

 cheers
 Bruce

 On Wed, 23 Jul
 2014, Weiss, Sarah wrote:

 Hi all,
 We are still struggling with this issue - any ideas? Here is some more
 information:

 - The surface that we are trying to label is the subject colin, as found
 here: http://mindboggle.info/data.html. We get two errors when loading in
 tksurfer
 (1) surfer: Tailairach xform file not found (ignored)
 (2) MRISreadVertexPosition(/Applications/.../subject/colin/surf/lh.org); No
 such file or directory
 However, the surface does load and can be manipulated (a label can be
 created manually and filled in).

 - We first tried to create a surface to overlay from our .nii file (which
 does load in Freeview) with mri_vol2surf. Our .nii had been converted to a
 .mgz with a corresponding .dat file using [COMMAND]. We tried --out_type
 paint (with file type .w) and --out_type mgh (with file type .mgh), but 
 both
 gave the same error:
 MRISread: file '.../surf/test-lh.w' has 0 vertices! Probably trying to use 
 a
 scalar data file as a surface!

 - We next tried mri_cor2label --i cluster1_roi.nii --l ./cluster1.label 
 --id
 1. In Freeview with our .nii file loaded, we verified that our volume of
 interest does have a value of 1, and unselected regions have a value of 0.
 It seems to run properly, finding unassigned vertices in label and 
 assigning
 vertex numbers to label. However, in tksurfer, when we select File  Load
 label value file  

[Freesurfer] Calculate average thickness mathematically?

2014-07-23 Thread Dorian P.
Hi all,

A while ago I asked how can I calculate the average thickness of the three
lateral temporal gyri, and Bruce kindly suggested to draw the label on
fsaverage and use transforms to calculate it for each subject. I may try to
do that, but given my time constrains and my level of knowledge of
Freesurger I thought an alternative method:

Say for example I have the inferior, middle, and superior temporal gyrus
with surface areas of 1000, 2000, and 3000. They also have an average
thickness of 2, 3, and 4mm, respectively. Given that these thicknesses are
distributed in those areas, I could weight each region accordingly and
calculate the average thickness as:

( (1000*2) + (2000*3) + (3000*4) ) / (1000+2000+3000) = 2/6000 = 3.33

Is this logic correct, and why yes or no?


Thank you in advance.
Dorian
TJU
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[Freesurfer] Mapping stats into volume with mri_label2vol

2014-07-23 Thread Jackie Lam
Hello all,

I am trying to map polar angle and eccentricity into the voxels underneath a 
particular label. For each vertex in the label file, I’ve put in the respective 
angle in the stats column. I then used mri_label2vol with the --label-stat flag 
to map the angle values into the volume. Comparing the angle values for each 
individual voxel and each vertex, I’ve noticed that some values that are in the 
voxels are not present in the vertices (ie a particular voxel might have a 
value of 0.5 but none of the vertices have that exact value). What happens when 
the values are mapped from the vertex into the volume? Is there some averaging 
or weighting process or something?

Many thanks,

jack


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Re: [Freesurfer] question regarding control point edits

2014-07-23 Thread Adam Mezher
Hi Bruce,
Yeah that was my thinking as well. So, if after running 500 subjects through FS 
and 100 need similar edits in the anterior temporal lobes, we will have no 
choice but to perform manual edits for each case individually?

Thanks for clarifying!
Adam Mezher



Hi Adam

I don't think so as how would we don't know where the anterior temporal 
lobe at that stage in the process.
Bruce


On 
Tue, 22 Jul 2014, Adam Mezher wrote:

 Hi FreeSurfer group,
 I was wondering if there is a way to include control points in my command
 line when I run recon-all. We are working with a large dataset and many of
 the subjects have underestimations in the anterior temporal lobes so it
 would be beneficial to expand the WM surface slightly on numerous cases
 rather than doing control point edits on each case individually.
 
 Thank you,
 Adam Mezher
 

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Re: [Freesurfer] question regarding control point edits

2014-07-23 Thread Bruce Fischl
Hi Adam

it depends on the source of the bias. If it is fixed in the coordinate 
system of the scanner you could correct a few, then try using 
mri_compute_bias and mri_apply_bias to see if it helps (it computes an 
average bias field in scanner coords, then applies it to a new dataset)

cheers
Bruce
On 
Wed, 23 Jul 2014, Adam Mezher wrote:

 
 Hi Bruce,
 
 Yeah that was my thinking as well. So, if after running 500 subjects through
  FS and 100 need similar edits in the anterior temporal lobes, we will have 
 no choice but to perform manual edits for each case individually?
 
 Thanks for clarifying!
 
 Adam Mezher
 
 
 
 
 
 Hi Adam
 
 I don't think so as how would we don't know where the anterior temporal 
 lobe at that stage in the process.
 Bruce
 
 
 On 
 Tue, 22 Jul 2014, Adam Mezher wrote:
 
  Hi FreeSurfer group,
  I was wondering if there is a way to include control points in my command
  line when I run recon-all. We are working with a large dataset and many of
  the subjects have underestimations in the anterior temporal lobes so it
  would be beneficial to expand the WM surface slightly on numerous cases
  rather than doing control point edits on each case individually.
  
  Thank you,
  Adam Mezher
  
 
 

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Re: [Freesurfer] Calculate average thickness mathematically?

2014-07-23 Thread Bruce Fischl
Hi Dorian

yes, that would work.

cheers
Bruce

On Wed, 23 Jul 2014, 
Dorian P. wrote:

 Hi all,
 A while ago I asked how can I calculate the average thickness of the three
 lateral temporal gyri, and Bruce kindly suggested to draw the label on
 fsaverage and use transforms to calculate it for each subject. I may try to
 do that, but given my time constrains and my level of knowledge of
 Freesurger I thought an alternative method:
 
 Say for example I have the inferior, middle, and superior temporal gyrus
 with surface areas of 1000, 2000, and 3000. They also have an average
 thickness of 2, 3, and 4mm, respectively. Given that these thicknesses are
 distributed in those areas, I could weight each region accordingly and
 calculate the average thickness as:
 
 ( (1000*2) + (2000*3) + (3000*4) ) / (1000+2000+3000) = 2/6000 = 3.33
 
 Is this logic correct, and why yes or no?
 
 
 Thank you in advance.
 Dorian
 TJU
 

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[Freesurfer] Question with QDEC annotations and mri_convert and mri_annotation2label

2014-07-23 Thread Tara Ann Miskovich
Hello,

I am trying to convert my significant cluster from qdec into a .gii file, and I 
have a couple of questions.

My mri_convert is not working, but it is my first time using it so I may be 
missing something, but it just keeps opening up the usage and gives no errors.

mris_convert --annot mc-z.abs.th13.sig.ocn.annot mc-z.abs.th13.sig.ocn.gii

Also, I was curious, how would one separate a annotation file created in qdec 
into separate label files with mri_annotation2label since there is no target 
subject which is required in he script.

Thanks!

Tara 

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Re: [Freesurfer] Questions regarding recon-all command

2014-07-23 Thread soumyabrata.dey
Hi Bruce,


Thanks a lot for replying.


The original data comes in .nii.gz format but when I unzipped it it become 
.hdr-.img (2 files). The freeSurfer is not understanding the .hdr-.img format 
but I am able to run it using .nii.gz format.


Thanks you again,

Soumya.



From: freesurfer-boun...@nmr.mgh.harvard.edu 
freesurfer-boun...@nmr.mgh.harvard.edu on behalf of Bruce Fischl 
fis...@nmr.mgh.harvard.edu
Sent: Wednesday, July 23, 2014 2:11 AM
To: Freesurfer support list
Subject: Re: [Freesurfer] Questions regarding recon-all command

Hi  Soumya

Can you include the full command and screen output of what you ran? Are you 
sure that they are nifti and not analyze? Analyze is to be avoided as it 
doesn't contain enough information to tell left from right
Cheers
Bruce


On Jul 22, 2014, at 8:01 PM, soumyabrata.dey 
soumyabrata@knights.ucf.edumailto:soumyabrata@knights.ucf.edu wrote:


?Hi All,


I am new to freeSurfer and need your guidance for some of the doubts I have.


I have installed freeSurfer in a windows 7 machine using virtualBox. I am 
trying to process some of my own sMRI data using freeSurfer software. The data 
is skull-stripped and has cerebellum. The format of the data is NIFTI (.hdr, 
.img). I am having some problem with running the recon-all command on the data 
as I am getting the error message saying filename.hdr is not found (I have 
also tried with inputting .img file).


My questions are:


  *   ?Is recon-all compatible with .hdr .img file format? I know it should run 
with .dcm and .nii files. If not what are my option?
  *   ?Do I need to set up any environment variable for the -i option of the 
recon-all like the SUBJECTS-DIR for -s option. I have tried inputting the full 
path of the input file. Also, I tried with moving to the directory of input 
file and run the command from there.

Thanks for your help,

Soumya.

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Re: [Freesurfer] Questions regarding recon-all command

2014-07-23 Thread Bruce Fischl

yes, there is no need to unzip it, we read gzipped nifti just fine
Bruce
On Wed, 
23 Jul 2014, soumyabrata.dey wrote:




Hi Bruce,


Thanks a lot for replying. 


The original data comes in .nii.gz format but when I unzipped it it become
.hdr-.img (2 files). The freeSurfer is not understanding the .hdr-.img
format but I am able to run it using .nii.gz format. 


Thanks you again,

Soumya.



From: freesurfer-boun...@nmr.mgh.harvard.edu
freesurfer-boun...@nmr.mgh.harvard.edu on behalf of Bruce Fischl
fis...@nmr.mgh.harvard.edu
Sent: Wednesday, July 23, 2014 2:11 AM
To: Freesurfer support list
Subject: Re: [Freesurfer] Questions regarding recon-all command  
Hi  Soumya

Can you include the full command and screen output of what you ran? Are you
sure that they are nifti and not analyze? Analyze is to be avoided as it
doesn't contain enough information to tell left from right
Cheers
Bruce


On Jul 22, 2014, at 8:01 PM, soumyabrata.dey
soumyabrata@knights.ucf.edu wrote:

  ​Hi All,


  I am new to freeSurfer and need your guidance for some of the
  doubts I have. 


  I have installed freeSurfer in a windows 7 machine using
  virtualBox. I am trying to process some of my own
  sMRI data using freeSurfer software. The data is
  skull-stripped and has cerebellum. The format of the data is
  NIFTI (.hdr, .img). I am having some problem with running the
  recon-all command on the data as I am getting the
  error message saying filename.hdr is not found (I have also
  tried with inputting .img file).


  My questions are:


   o  ​Is recon-all compatible with .hdr .img file format? I know
  it should run with .dcm and .nii files. If not what are my
  option?
   o  ​Do I need to set up any environment variable for the -i
  option of the recon-all like the SUBJECTS-DIR for -s option.
  I have tried inputting the full path of the input file.
  Also, I tried with moving to the directory of input file and
  run the command from there.

  Thanks for your help,

  Soumya.

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Re: [Freesurfer] Calculate average thickness mathematically?

2014-07-23 Thread Dorian P.
Thanks Bruce, this is going to be very helpful.

One thing I have not understood from the statistics freesurfer outputs is
why average thickness is not exactly the ratio between volume and surface
area. This is just a small curiosity though.

Best regards.
Dorian
On Jul 23, 2014 3:40 PM, Bruce Fischl fis...@nmr.mgh.harvard.edu wrote:

 Hi Dorian

 yes, that would work.

 cheers
 Bruce

 On Wed, 23 Jul 2014,
 Dorian P. wrote:

  Hi all,
  A while ago I asked how can I calculate the average thickness of the
 three
  lateral temporal gyri, and Bruce kindly suggested to draw the label on
  fsaverage and use transforms to calculate it for each subject. I may try
 to
  do that, but given my time constrains and my level of knowledge of
  Freesurger I thought an alternative method:
 
  Say for example I have the inferior, middle, and superior temporal gyrus
  with surface areas of 1000, 2000, and 3000. They also have an average
  thickness of 2, 3, and 4mm, respectively. Given that these thicknesses
 are
  distributed in those areas, I could weight each region accordingly and
  calculate the average thickness as:
 
  ( (1000*2) + (2000*3) + (3000*4) ) / (1000+2000+3000) = 2/6000 = 3.33
 
  Is this logic correct, and why yes or no?
 
 
  Thank you in advance.
  Dorian
  TJU
 
 
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 is
 addressed. If you believe this e-mail was sent to you in error and the
 e-mail
 contains patient information, please contact the Partners Compliance
 HelpLine at
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 error
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 properly
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