[Freesurfer] Question about volumetric group analysis

2011-05-17 Thread Fernanda Palhano
Hello,

I have two questions about volumetric analysis:
First: Are the volume measures in the files aseg.stats or ?h.aparc.stats
(Gray Matter Volume) already normalized by ICV? Or should I divide
all the structure volumes which I want to compare between subjects by the
ICV of each subject?
Second: When comparing gray matter volumes the correct is use the ICV or the
total cortical gray matter volume to normalize? And if I have
to use the total cortical gray matter volume, how can I obtain it? I know
that this information is in the aseg.stats file, but I was wondering if I
can use asegstats2table to extract it.


Thank you very much,

Fernanda
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[Freesurfer] mri_segstats error

2011-05-16 Thread Fernanda Palhano
Hello,

I'm trying to use mri_segstats to obtain some subcortical volume stats. When
running the command line both in the tutorial data or in my own data, I have
the follow error: ERROR: could not open stats/aseg.stats for writing

[Fernanda-Palhanos-Mac-Pro:buckner_data/tutorial_subjs/group_analysis_tutorial]
fernandapalhano% mri_segstats --seg mri/aseg.mgz --sum stats/aseg.stats --pv
mri/norm.mgz --ctab-default --excludeid 0 --brain-vol-from-seg --brainmask
mri/brainmask.mgz --in mri/norm.mgz --in-intensity-name norm
--in-intensity-units MR --etiv --subject 004
Using defalt ctab /Applications/freesurfer/FreeSurferColorLUT.txt

$Id: mri_segstats.c,v 1.69.2.1 2010/07/26 16:19:29 greve Exp $
cwd
cmdline mri_segstats --seg mri/aseg.mgz --sum stats/aseg.stats --pv
mri/norm.mgz --ctab-default --excludeid 0 --brain-vol-from-seg --brainmask
mri/brainmask.mgz --in mri/norm.mgz --in-intensity-name norm
--in-intensity-units MR --etiv --subject 004
sysname  Darwin
hostname Fernanda-Palhanos-Mac-Pro.local
machine  i386
user fernandapalhano
atlas_icv (eTIV) = 1792580 mm^3(det: 1.086761 )
ERROR: could not open stats/aseg.stats for writing

Any ideas?

Thanks,
Fernanda
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Re: [Freesurfer] Interpreting results from Qdec

2011-05-05 Thread Fernanda Palhano
Hello,

I have a follow up question about using mri_segstats. What is the difference
between to use mri_segstats --in
SUBJECT/surf/lh.thickness.fwhm10.fsaverage.mgh --slabel fsaverage lh
ROI-drawn-on-QDEC-brain.label --avgwf lhSupTempctrls.txt and to use
mri_label2label --srclabel fsaverage/label/ROI-drawn-on-QDEC-brain.label
--srcsubject fsaverage --trgsubject SUBJECT --trglabel
SUBJECT/label/ROI-drawn-on-QDEC-brain.label --regmethod surface --hemi lh
and then mris_anatomical_stats -l
SUBJECT/label/ROI-drawn-on-QDEC-brain.label -t lh.thickness -b -f
SUBJECT/stats/ROI-drawn-on-QDEC-brain.label.stats SUBJECT lh.
Should I have the same results using both?

Thanks,
Fernanda

On Wed, Apr 27, 2011 at 2:12 PM, Gallen, Courtney (NIH/NIDA) [F] <
galle...@nida.nih.gov> wrote:

> Hi Doug
>
> Have a follow up question regarding significance levels in Qdec related to
> extracting means from ROIs, etc. I'm posting to the entire mailing list in
> case anyone else has ideas.
>
> Basically, I extracted mean volumes (from my set of participants) of ROIs
> for which Qdec reports an interaction between two variables. I want to look
> at these mean volumes in something like SPSS to see what is driving the
> interaction/generate figures, etc.
>
> I'm having an issue with one such ROI, in that when I extract the mean
> volumes and look for this interaction in SPSS, it is not significant (p ~
> 0.5, so really not significant at all). Is there any way that an ROI could
> be significant in Qdec (p of 0.05, Monte Carlo sim), but not in SPSS when I
> extract means for each participant?
>
> Let me know if this isn't clear. Any thoughts would be appreciated!
> Courtney
>
>
> Courtney Gallen
> Post-baccalaureate IRTA
> Neuroimaging Research Branch
> National Institute on Drug Abuse (IRP)
> 251 Bayview Blvd
> Suite 200
> Baltimore, MD 21224
> Tel: (443) 740-2631
>
> -Original Message-
> From: Douglas Greve [mailto:gr...@nmr.mgh.harvard.edu]
> Sent: Tuesday, April 26, 2011 1:06 PM
> To: Gallen, Courtney (NIH/NIDA) [F]
> Subject: Re: [Freesurfer] Interpreting results from Qdec
>
> Those are the values outside (first column) and inside (2nd col) the
> ROI. You can add "--id 1" to only report the values inside the ROI.
>
> doug
>
> On 4/26/11 1:02 PM, Gallen, Courtney (NIH/NIDA) [F] wrote:
> > It worked!
> > Now, last question. The txt file has two separate values (?averages).
> What do they each represent?
> >
> > -Original Message-
> > From: Douglas Greve [mailto:gr...@nmr.mgh.harvard.edu]
> > Sent: Tuesday, April 26, 2011 12:53 PM
> > To: Gallen, Courtney (NIH/NIDA) [F]
> > Subject: Re: [Freesurfer] Interpreting results from Qdec
> >
> > try "--slabel fsaverage lh ROI-drawn-on-QDEC-brain.label"
> >
> >
> > On 4/26/11 12:52 PM, Gallen, Courtney (NIH/NIDA) [F] wrote:
> >> Actually, I hope I'm wrong about what I thought below. If I specify
> individual subject's label and run:
> >>
> >> mri_segstats --in
> /prot/Mprot/CTQ/freesurferDone/8579/surf/lh.volume.fwhm10.fsaverage.mgh
> --slabel 8579 lh
> /prot/Mprot/CTQ/freesurferDone/8579/label/lh.SupTemp_CTQxBDNFctrls.label
> --avgwf lhSupTempctrls.txt
> >>
> >> I still get the same error about dimension mismatch
> >>
> >>
> >> -Original Message-
> >> From: Gallen, Courtney (NIH/NIDA) [F]
> >> Sent: Tuesday, April 26, 2011 12:43 PM
> >> To: 'Douglas Greve'
> >> Subject: RE: [Freesurfer] Interpreting results from Qdec
> >>
> >> Ah I think I see the problem now.
> >>
> >> The label I originally specified is the general label created from the
> ROI drawing in Qdec. Instead, I should use the individual subject's label
> (from 'Map Label to Subjects') instead, right?
> >>
> >> Thank you!
> >>
> >> -Original Message-
> >> From: Douglas Greve [mailto:gr...@nmr.mgh.harvard.edu]
> >> Sent: Tuesday, April 26, 2011 12:38 PM
> >> To: Gallen, Courtney (NIH/NIDA) [F]
> >> Subject: Re: [Freesurfer] Interpreting results from Qdec
> >>
> >> You have to use the subject that matches the input, fsaverage in this
> >> case. How did you define your label? If you defined it on subject 123,
> >> you can transfer it to fsaverage using mri_label2label.
> >> doug
> >>
> >> On 4/26/11 12:28 PM, Gallen, Courtney (NIH/NIDA) [F] wrote:
> >>> Not a problem at all!
> >>>
> >>> If I use --in, I get an error saying that there is a dimension mismatch
> between input volume and seg.
> >>>
> >>> Any other thoughts? Thanks again!
> >>>
> >>> -Original Message-
> >>> From: Douglas Greve [mailto:gr...@nmr.mgh.harvard.edu]
> >>> Sent: Tuesday, April 26, 2011 11:40 AM
> >>> To: Gallen, Courtney (NIH/NIDA) [F]
> >>> Subject: Re: [Freesurfer] Interpreting results from Qdec
> >>>
> >>>
> >>> Hi Courtney, sorry for the delay. If more than a few days go by without
> >>> a response, feel free to repost -- I promise not to get annoyed!
> >>> Sometimes, things get pushed up and out of my mail browser window, and
> I
> >>> often don't get back to them. Sorry!
> >>>
> >>> To answer your question, try using -

[Freesurfer] mri_surfcluster question

2011-05-02 Thread Fernanda Palhano
Hello,

I'm running a cortical thickness analysis using Qdec. When I do Find
Clusters and Go to Max, I obtain 9 clusters (min threshold 3, max threshold
5):

Generating cluster stats using min threshold of 3...
Found 9 clusters
Contrast: 'lh-Diff-grupo1-grupo2-Intercept-thickness', 10fwhm, DOF: 30
ClusterNo  Max   VtxMax  Size(mm2)   TalX   TalY   TalZ NVtxs Annotation
-  ---   --  -          - --
18.8371   89238  5.07   -28.2   12.0  -14.5   16  insula
2   -5.3729   64233428.45   -35.4   50.5   -8.5  583  parsorbitalis
3   -5.1786   73273192.98-8.0   46.4  -13.0  292
medialorbitofrontal
4   -3.8763  121706 38.22   -19.6   61.01.5   53
rostralmiddlefrontal
5   -3.8612   93042 93.07   -23.8   40.2  -11.0  153
lateralorbitofrontal
6   -3.5732   14941 41.60-7.0   41.1  -22.2   79
medialorbitofrontal
73.3511  160348  0.34   -30.3   10.7  -14.11  insula
83.3502  111085 31.96   -33.8  -13.2   -4.6   68  insula
9   -3.2412   99299 19.05   -34.6   47.2   18.4   26
rostralmiddlefrontal


Why when using the command line mri_surfcluster with the same thresholds I
do not have the same results (same clusters)?

# Cluster Growing Summary (mri_surfcluster)
# $Id: mri_surfcluster.c,v 1.48 2010/04/12 17:36:58 greve Exp $
# $Id: mrisurf.c,v 1.678 2010/05/28 21:22:21 rpwang Exp $
# CreationTime 2011/05/02-19:07:52-GMT
# cmdline mri_surfcluster --in
qdec/lh_g1_g2/lh-Diff-grupo1-grupo2-Intercept-thickness/sig.mgh --subject
fsaverage --hemi lh --thmin 3 --thmax 5 --sign abs --annot aparc --sum
summary_3_5 --olab lh_g1_g2
# cwd /Applications/freesurfer/subjects/dados
# sysname  Darwin
# hostname Fernanda-Palhanos-Mac-Pro.local
# machine  i386
# FixVertexAreaFlag 1
# FixSurfClusterArea 1
#
# Input  qdec/lh_g1_g2/lh-Diff-grupo1-grupo2-Intercept-thickness/sig.mgh
# Frame Number  0
# srcsubj fsaverage
# hemi lh
# surface white
# annot aparc
# SUBJECTS_DIR /Applications/freesurfer/subjects/dados
# Minimum Threshold 3
# Maximum Threshold 5
# Threshold Signabs
# AdjustThreshWhenOneTail 1
# Area Threshold0 mm^2
# Overall max 8.83713 at vertex 89238
# Overall min -5.37286 at vertex 64233
# NClusters  12
# Total Cortical Surface Area 65416.6 (mm^2)
# FixMNI = 1
#
# ClusterNo  Max   VtxMax   Size(mm^2)  TalX   TalY   TalZNVtxs   Annot
   1   -4.995   29209398.78-33.5   48.6  -10.8   548
rostralmiddlefrontal
   2   -4.995   16744185.39 -8.3   45.3  -12.5   283
medialorbitofrontal
   34.914   28205  0.34-28.4   11.8  -11.8 1  insula
   44.850   22061  2.37-26.89.7  -13.8 8  insula
   54.512   52766  0.38-29.8   11.0  -11.4 1  insula
   6   -3.876  121706 38.22-19.4   59.1   -1.653
rostralmiddlefrontal
   7   -3.861   93042 93.07-23.5   38.5  -11.2   153
lateralorbitofrontal
   83.691   89239  0.68-27.5   11.7  -12.5 2  insula
   9   -3.573   14941 41.60 -7.0   38.8  -20.679
medialorbitofrontal
  103.351  160348  0.34-29.99.7  -12.3 1  insula
  113.350  111085 31.96-33.5  -12.9   -3.268  insula
  12   -3.241   99299 19.05-34.2   46.6   14.726
rostralmiddlefrontal

In the right hemisphere I have the same problem:


Generating cluster stats using min threshold of 3...
Found 6 clusters
Contrast: 'rh-Diff-grupo1-grupo2-Intercept-thickness', 10fwhm, DOF: 30
ClusterNo  Max   VtxMax  Size(mm2)   TalX   TalY   TalZ NVtxs Annotation
-  ---   --  -          - --
1   16.5175   22102  2.8933.5   10.7  -13.07  insula
26.0843  160464  1.0830.8   13.1  -13.13  insula
3   -5.9997   62430  1.0836.13.3  -19.13  insula
4   -3.3483   60593 24.7535.9   55.5   -7.6   36
rostralmiddlefrontal
53.2156   23631 10.7819.8  -65.11.5   15  lingual
63.1968  120630  0.2829.1   11.5  -15.81  insula


cmdline mri_surfcluster --in
qdec/rh_g1_g2/rh-Diff-grupo1-grupo2-Intercept-thickness/sig.mgh --subject
fsaverage --hemi rh --thmin 3 --thmax 5 --sign abs --annot aparc --sum
rh_summary_3_5 --olab rh_g1_g2
# cwd /Applications/freesurfer/subjects/dados
# sysname  Darwin
# hostname Fernanda-Palhanos-Mac-Pro.local
# machine  i386
# FixVertexAreaFlag 1
# FixSurfClusterArea 1
#
# Input  qdec/rh_g1_g2/rh-Diff-grupo1-grupo2-Intercept-thickness/sig.mgh
# Frame Number  0
# srcsubj fsaverage
# hemi rh
# surface white
# annot aparc
# SUBJECTS_DIR /Applications/freesurfer/subjects/dados
# Minimum Threshold 3
# Maximum Thre

Re: [Freesurfer] overlay sig.mgh onto T1 vol

2011-02-21 Thread Fernanda Palhano
Hi Bruce,

Thank you for the prompt reply.
I tried using mri_surf2vol :
mri_surf2vol --surfval
qdec/g1_g2_lh_06out/lh-Diff-grupo1-grupo2-Intercept-thickness/sig.mgh
--identity fsaverage --template fsaverage/mri/T1.mgz --hemi lh --o
teste_sig.mgz
gdiagno = -1
Using identity matrix for registration
Qa2v: SurfXYZ to VolCRS: --
-1.000   0.000   0.000   128.000;
 0.000   0.000  -1.000   128.000;
 0.000   1.000   0.000   128.000;
 0.000   0.000   0.000   1.000;
--
subjects dir   /Applications/freesurfer/subjects/dados_Riba/
surface value path
qdec/g1_g2_lh_06out/lh-Diff-grupo1-grupo2-Intercept-thickness/sig.mgh
hemi   lh
mksurfmask 0
projfrac   0
outvol   path  teste_sig.mgz
template path  fsaverage/mri/T1.mgz
--- Anat2Vol Registration (TkReg)
 1.000   0.000   0.000   0.000;
 0.000   1.000   0.000   0.000;
 0.000   0.000   1.000   0.000;
 0.000   0.000   0.000   1.000;
-
height = 256
 width = 256
 depth = 256
 xsize = 1.00
 ysize = 1.00
 zsize = 1.00
  cdc  = -1.00 0.00 0.00
  rdc  = 0.00 0.00 -1.00
  sdc  = 0.00 1.00 0.00
  xyz0 = 0.00 0.00 0.00
Gdiag_no  -1
Reading surface
/Applications/freesurfer/subjects/dados_Riba//fsaverage/surf/lh.white
Done reading source surface
INFO: reading
qdec/g1_g2_lh_06out/lh-Diff-grupo1-grupo2-Intercept-thickness/sig.mgh as MGH
Done loading source values (nvtxs = 163842)
INFO: mapping vertices to closest voxel
INFO: resampling surface to volume
INFO: sampled 73965 voxels in the volume
INFO: writing output volume to teste_sig.mgz
done

And then, use tkmedit to view it:
[Fernanda-Palhanos-Mac-Pro:freesurfer/subjects/dados_Riba] fernandapalhano%
tkmedit fsaverage teste_sig.mgz
mghRead(/Applications/freesurfer/subjects/dados_Riba//fsaverage/mri/teste_sig.mgz,
-1): could not open file


  Error: Loading volume teste_sig.mgz

  Couldn't read the anatomical volume.

  Tkmedit couldn't read the volume you specified.
  This could be because the image format wasn't recognized,
  or it couldn't find the proper header,
  or the file(s) were unreadable,
  or it was the wrong size.

Am I using wrong parameters in mri_surf2vol?

Thanks again,
Fernanda

On Mon, Feb 21, 2011 at 5:30 PM, Bruce Fischl wrote:

> Hi Fernanda
>
> if that's a surface overlay you'll need to sample it into the volume with
> mri_surf2vol
>
> cheers
> Bruce
>
>
>
> On Mon, 21 Feb 2011, Fernanda Palhano wrote:
>
>  Hello,
>>
>> Does anyone knows if is it possible to overlay the surface file sig.mgh
>> onto
>> a T1 vol?
>> I tried the command:
>> tkmedit fsaverage T1.mgz -fthresh 2 -fmax 5 -overlay
>> qdec/rh-Diff-grupo1-grupo2-Intercept-thickness/sig.mgh (where 2 and 5 were
>> the thresholds I used in qdec),
>> but it seems like I have only one point of the sig.mgh (in the slice 128)
>> which is outside the volume.
>> There's another way to do this?
>>
>> Thanks for the help!
>>
>> Fernanda
>>
>>
>
> The information in this e-mail is intended only for the person to whom it
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[Freesurfer] overlay sig.mgh onto T1 vol

2011-02-21 Thread Fernanda Palhano
Hello,

Does anyone knows if is it possible to overlay the surface file sig.mgh onto
a T1 vol?
I tried the command:
tkmedit fsaverage T1.mgz -fthresh 2 -fmax 5 -overlay
qdec/rh-Diff-grupo1-grupo2-Intercept-thickness/sig.mgh (where 2 and 5 were
the thresholds I used in qdec),
but it seems like I have only one point of the sig.mgh (in the slice 128)
which is outside the volume.
There's another way to do this?

Thanks for the help!

Fernanda
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[Freesurfer] Error FsFast Tutorial

2011-01-10 Thread Fernanda Palhano
Hi,

I am trying to run the FsFast tutorial, but I am getting the following
error:


Fernanda-Palhanos-Mac-Pro:fsfast/fsfast-tutorial/fb1-analysis-study]
fernandapalhano% tkmedit-sess -s mgh-101.1 -a sm-gamma-fwhm5 -c odd-v-0
-aparc+aseg
tmp: Subscript out of range.
tmp: Subscript out of range.
tmp: Subscript out of range.
tmp: Subscript out of range.
ERROR: cannot find
/Users/fernandapalhano/Data/fsfast/fsfast-tutorial/fb1-analysis-study/mgh-101.1/bold/register.dof6.dat
[Fernanda-Palhanos-Mac-Pro:fsfast/fsfast-tutorial/fb1-analysis-study]
fernandapalhano% tksurfer-sess -s mgh-101.1 -a sm-gamma-fwhm5 -c odd-v-0
-hemi lh -aparc
tmp: Subscript out of range.
tmp: Subscript out of range.
tmp: Subscript out of range.
tmp: Subscript out of range.
$Id: tksurfer-sess,v 1.15.2.1 2010/07/14 16:08:04 greve Exp $
Darwin Fernanda-Palhanos-Mac-Pro.local 10.4.0 Darwin Kernel Version 10.4.0:
Fri Apr 23 18:28:53 PDT 2010; root:xnu-1504.7.4~1/RELEASE_I386 i386
Mon Jan 10 15:08:15 BRT 2011
/Applications/freesurfer/bin/tksurfer

list odd-v-0
con/map odd-v-0
/Users/fernandapalhano/Data/fsfast/fsfast-tutorial/fb1-analysis-study/mgh-101.1/bold/sm-gamma-fwhm5/odd-v-0/sig.nii
maplist
/Users/fernandapalhano/Data/fsfast/fsfast-tutorial/fb1-analysis-study/mgh-101.1/bold/sm-gamma-fwhm5/odd-v-0/sig.nii
mri_concat
/Users/fernandapalhano/Data/fsfast/fsfast-tutorial/fb1-analysis-study/mgh-101.1/bold/sm-gamma-fwhm5/odd-v-0/sig.nii
--o
/Users/fernandapalhano/Data/fsfast/fsfast-tutorial/fb1-analysis-study/mgh-101.1/bold/sm-gamma-fwhm5/tksconcat.nii
ninputs = 1
Checking inputs
nframestot = 1
Allocing output
Done allocing
nframes = 1
Writing to
/Users/fernandapalhano/Data/fsfast/fsfast-tutorial/fb1-analysis-study/mgh-101.1/bold/sm-gamma-fwhm5/tksconcat.nii
ERROR: cannot find
/Users/fernandapalhano/Data/fsfast/fsfast-tutorial/fb1-analysis-study/mgh-101.1/bold/register.dof6.dat

I checked all the mgh files in fb1-analysis-study and any of them have
register.dof6.dat, there is only register.dat.
Where can I obtain these file?

Thanks in advance,
Fernanda Palhano
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[Freesurfer] Clusters - Qdec 1.4

2010-10-05 Thread Fernanda Palhano
Hello All,

Does anyone know if it's possible to automatically create labels for the
clusters found
with the new feature in Qdec 1.4, *Find Clusters and Goto Max*?
I want to map the clusters onto all my subjects and then use  *
mris_anatomical_stats* to get average thickness and st. deviation for
each one.

Thanks in advance,
Fernanda Palhano
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[Freesurfer] ERROR: _FindFacePath

2010-08-17 Thread Fernanda Palhano
Hello,

When trying running recon-all I am getting the following error:

#...@# Fix Topology rh Sat Aug 14 04:15:31 BRT 2010

ERROR: _FindFacePath: could not find path!
Darwin Fernanda-Palhanos-Mac-Pro.local 10.4.0 Darwin Kernel Version 10.4.0:
Fri Apr 23 18:28:53 PDT 2010; root:xnu-1504.7.4~1/RELEASE_I386 i386

recon-all exited with ERRORS at Sat Aug 14 04:26:14 BRT 2010

I've tryed twice, but i get the same error.
Could anyone help me?

Thanks in advance,
Fernanda
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[Freesurfer] Help on QDEC: Obtainign mean, sd and p-values from glm result

2010-07-07 Thread Fernanda Palhano
Hello,


I've just started using freesurfer. I've reading the mail list wich helps me
a lot, but
I can't find how I can obtain a table with the values of my glm analysis.
For exemple, in the Qdec's tutorial I have a surface that answer the
question "Does
the correlation between thickness and age differ from zero?" It's possible
to see this data like
a table:
region   mean sdp-value
precentral   xx x   

Or at least obtain those values individually?

thank you very much in advance,
Fernanda
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The information in this e-mail is intended only for the person to whom it is
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but does not contain patient information, please contact the sender and properly
dispose of the e-mail.