Re: [Freesurfer] glmfit with aseg table: view surface overlay

2021-04-18 Thread Douglas N. Greve

Not easily. You could do something in matlab, eg
aseg = MRIread('aseg.mgz');
sig = aseg;
sig.vol = zeros(seg.volsize);
ind = find(aseg.vol == 17); % left hippocampus
sig.vol(ind) = SigValueForLeftHippo; % from your table
% Do the same with the rest of your segmentations
MRIwrite(sig,'mysig.mgz')


On 4/16/2021 4:09 PM, Griem, Julia wrote:


External Email - Use Caution

External Email - Use Caution

Hi Doug,

Ok, that’s fine. Is it possible to visualize subcortical volume 
differences in any way?


Thank you!
Julia

/NIHR Maudsley Biomedical Research Centre PhD Student/

/Forensic & Neurodevelopmental Sciences/

/Institute of Psychiatry, Psychology & Neuroscience/

/King’s College London/

/16 De Crespigny Park, Camberwell/

/London, SE5 8AB/

*From:*freesurfer-boun...@nmr.mgh.harvard.edu 
 *On Behalf Of *Douglas N. Greve

*Sent:* 16 April 2021 16:11
*To:* freesurfer@nmr.mgh.harvard.edu
*Subject:* Re: [Freesurfer] glmfit with aseg table: view surface overlay

Are you trying to correct the table? You can't do that with 
mri_gtlmfit-sim. That looks for clusters, which does not make sense 
when you are using table data. There are a couple of ways you can do 
it. One is with bonerfonni correction (ie, multiply the p-value by the 
number of ROIs) or with FDR.


On 4/15/2021 11:13 AM, Griem, Julia wrote:

*External Email - Use Caution *

*External Email - Use Caution *

Hi Doug,

Thank you. Makes sense. Is there any way to visualize significance
overlays for the subcortical volumes?

I’ve also moved on to the correction for multiple comparisons in
my subcortical volumes, using:

mri_glmfit-sim -- glmdir roi.binarygroup.glmdir --perm 1000 1.3
abs --cwp 0.05 --2spaces --bg 1

This did not create any new files in the Contrast subdirectories,
but the log file does say that it was run and finished. Can I find
a table with corrected p-values anywhere? Or would it make more
sense to move the aseg_table into a different stats package and
calculate my GLM there, correcting for multiple comparisons
post-hoc (manually)?

Thank you so much

Julia

/NIHR Maudsley Biomedical Research Centre PhD Student/

/Forensic & Neurodevelopmental Sciences/

/Institute of Psychiatry, Psychology & Neuroscience/

/King’s College London/

/16 De Crespigny Park, Camberwell/

/London, SE5 8AB/

*From:*freesurfer-boun...@nmr.mgh.harvard.edu
<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>

<mailto:freesurfer-boun...@nmr.mgh.harvard.edu> *On Behalf Of
*Douglas N. Greve
*Sent:* 15 April 2021 15:14
*To:* freesurfer@nmr.mgh.harvard.edu
<mailto:freesurfer@nmr.mgh.harvard.edu>
    *Subject:* Re: [Freesurfer] glmfit with aseg table: view surface
overlay

The input is a table, so the output is a table (the sig.mgh file
will just have the number of values that are in the table; this is
not a "volume" you can look at in FV). Look in the sig.table.dat
file for the sigs.

On 4/13/2021 8:41 AM, Griem, Julia wrote:

*External Email - Use Caution *

*External Email - Use Caution *

Dear Freesurfer team,

I am comparing subcortical volumes (aseg table) in 2 groups
with no other variables. Based on the GLM lecture slides from
the course I attended, I ran the following glmfit command:

mri_glmfit

--table aseg_table.txt

--fsgd FSGD/DemographicsSubcortBinaryGroup.fsgd

--C Contrasts/HC-ASPD.mtx

--C Contrasts/ASPD-HC.mtx

--glmdir roi.binarygroup.glmdir

It created all the correct output and subdirectories.

I then used

freeview -f
$SUBJECTS_DIR/fsaverage/surf/lh.inflated:overlay=ASPD-HC/sig.mgh

but an error saying "failed to load surface overlay" comes up
and the terminal error message is "number of vertices in
overlay data does not match with surface". I assume this is
because I am trying to load a 'whole-brain' sig.mgh onto a lh
surface? Do I have to change the glmfit command to specify
hemispheres, and if so, how do I do that since I do not want
to look at cortical but subcortical data? Or alternatively,
can I load both hemispheres into freeview so the overlay
sig.mgh fits?

Thank you so much,
Julia




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Re: [Freesurfer] glmfit with aseg table: view surface overlay

2021-04-16 Thread Griem, Julia
External Email - Use Caution

Hi Doug,

Ok, that's fine. Is it possible to visualize subcortical volume differences in 
any way?

Thank you!
Julia

NIHR Maudsley Biomedical Research Centre PhD Student
Forensic & Neurodevelopmental Sciences
Institute of Psychiatry, Psychology & Neuroscience
King's College London
16 De Crespigny Park, Camberwell
London, SE5 8AB

From: freesurfer-boun...@nmr.mgh.harvard.edu 
 On Behalf Of Douglas N. Greve
Sent: 16 April 2021 16:11
To: freesurfer@nmr.mgh.harvard.edu
Subject: Re: [Freesurfer] glmfit with aseg table: view surface overlay

Are you trying to correct the table? You can't do that with mri_gtlmfit-sim. 
That looks for clusters, which does not make sense when you are using table 
data. There are a couple of ways you can do it. One is with bonerfonni 
correction (ie, multiply the p-value by the number of ROIs) or with FDR.
On 4/15/2021 11:13 AM, Griem, Julia wrote:

External Email - Use Caution

External Email - Use Caution
Hi Doug,

Thank you. Makes sense. Is there any way to visualize significance overlays for 
the subcortical volumes?

I've also moved on to the correction for multiple comparisons in my subcortical 
volumes, using:

mri_glmfit-sim -- glmdir roi.binarygroup.glmdir --perm 1000 1.3 abs --cwp 0.05 
--2spaces --bg 1

This did not create any new files in the Contrast subdirectories, but the log 
file does say that it was run and finished. Can I find a table with corrected 
p-values anywhere? Or would it make more sense to move the aseg_table into a 
different stats package and calculate my GLM there, correcting for multiple 
comparisons post-hoc (manually)?

Thank you so much
Julia

NIHR Maudsley Biomedical Research Centre PhD Student
Forensic & Neurodevelopmental Sciences
Institute of Psychiatry, Psychology & Neuroscience
King's College London
16 De Crespigny Park, Camberwell
London, SE5 8AB

From: 
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 On Behalf Of Douglas N. Greve
Sent: 15 April 2021 15:14
To: freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] glmfit with aseg table: view surface overlay

The input is a table, so the output is a table (the sig.mgh file will just have 
the number of values that are in the table; this is not a "volume" you can look 
at in FV). Look in the sig.table.dat file for the sigs.
On 4/13/2021 8:41 AM, Griem, Julia wrote:

External Email - Use Caution

External Email - Use Caution
Dear Freesurfer team,

I am comparing subcortical volumes (aseg table) in 2 groups with no other 
variables. Based on the GLM lecture slides from the course I attended, I ran 
the following glmfit command:
mri_glmfit
--table aseg_table.txt
--fsgd FSGD/DemographicsSubcortBinaryGroup.fsgd
--C Contrasts/HC-ASPD.mtx
--C Contrasts/ASPD-HC.mtx
--glmdir roi.binarygroup.glmdir

It created all the correct output and subdirectories.

I then used
freeview -f $SUBJECTS_DIR/fsaverage/surf/lh.inflated:overlay=ASPD-HC/sig.mgh

but an error saying "failed to load surface overlay" comes up and the terminal 
error message is "number of vertices in overlay data does not match with 
surface". I assume this is because I am trying to load a 'whole-brain' sig.mgh 
onto a lh surface? Do I have to change the glmfit command to specify 
hemispheres, and if so, how do I do that since I do not want to look at 
cortical but subcortical data? Or alternatively, can I load both hemispheres 
into freeview so the overlay sig.mgh fits?

Thank you so much,
Julia




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Re: [Freesurfer] glmfit with aseg table: view surface overlay

2021-04-16 Thread Douglas N. Greve
Are you trying to correct the table? You can't do that with 
mri_gtlmfit-sim. That looks for clusters, which does not make sense when 
you are using table data. There are a couple of ways you can do it. One 
is with bonerfonni correction (ie, multiply the p-value by the number of 
ROIs) or with FDR.


On 4/15/2021 11:13 AM, Griem, Julia wrote:


External Email - Use Caution

External Email - Use Caution

Hi Doug,

Thank you. Makes sense. Is there any way to visualize significance 
overlays for the subcortical volumes?


I’ve also moved on to the correction for multiple comparisons in my 
subcortical volumes, using:


mri_glmfit-sim -- glmdir roi.binarygroup.glmdir --perm 1000 1.3 abs 
--cwp 0.05 --2spaces --bg 1


This did not create any new files in the Contrast subdirectories, but 
the log file does say that it was run and finished. Can I find a table 
with corrected p-values anywhere? Or would it make more sense to move 
the aseg_table into a different stats package and calculate my GLM 
there, correcting for multiple comparisons post-hoc (manually)?


Thank you so much

Julia

/NIHR Maudsley Biomedical Research Centre PhD Student/

/Forensic & Neurodevelopmental Sciences/

/Institute of Psychiatry, Psychology & Neuroscience/

/King’s College London/

/16 De Crespigny Park, Camberwell/

/London, SE5 8AB/

*From:*freesurfer-boun...@nmr.mgh.harvard.edu 
 *On Behalf Of *Douglas N. Greve

*Sent:* 15 April 2021 15:14
*To:* freesurfer@nmr.mgh.harvard.edu
*Subject:* Re: [Freesurfer] glmfit with aseg table: view surface overlay

The input is a table, so the output is a table (the sig.mgh file will 
just have the number of values that are in the table; this is not a 
"volume" you can look at in FV). Look in the sig.table.dat file for 
the sigs.


On 4/13/2021 8:41 AM, Griem, Julia wrote:

*External Email - Use Caution *

*External Email - Use Caution *

Dear Freesurfer team,

I am comparing subcortical volumes (aseg table) in 2 groups with
no other variables. Based on the GLM lecture slides from the
course I attended, I ran the following glmfit command:

mri_glmfit

--table aseg_table.txt

--fsgd FSGD/DemographicsSubcortBinaryGroup.fsgd

--C Contrasts/HC-ASPD.mtx

--C Contrasts/ASPD-HC.mtx

--glmdir roi.binarygroup.glmdir

It created all the correct output and subdirectories.

I then used

freeview -f
$SUBJECTS_DIR/fsaverage/surf/lh.inflated:overlay=ASPD-HC/sig.mgh

but an error saying "failed to load surface overlay" comes up and
the terminal error message is "number of vertices in overlay data
does not match with surface". I assume this is because I am trying
to load a 'whole-brain' sig.mgh onto a lh surface? Do I have to
change the glmfit command to specify hemispheres, and if so, how
do I do that since I do not want to look at cortical but
subcortical data? Or alternatively, can I load both hemispheres
into freeview so the overlay sig.mgh fits?

Thank you so much,
Julia



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Re: [Freesurfer] glmfit with aseg table: view surface overlay

2021-04-15 Thread Griem, Julia
External Email - Use Caution

Hi Doug,

Thank you. Makes sense. Is there any way to visualize significance overlays for 
the subcortical volumes?

I've also moved on to the correction for multiple comparisons in my subcortical 
volumes, using:

mri_glmfit-sim -- glmdir roi.binarygroup.glmdir --perm 1000 1.3 abs --cwp 0.05 
--2spaces --bg 1

This did not create any new files in the Contrast subdirectories, but the log 
file does say that it was run and finished. Can I find a table with corrected 
p-values anywhere? Or would it make more sense to move the aseg_table into a 
different stats package and calculate my GLM there, correcting for multiple 
comparisons post-hoc (manually)?

Thank you so much
Julia

NIHR Maudsley Biomedical Research Centre PhD Student
Forensic & Neurodevelopmental Sciences
Institute of Psychiatry, Psychology & Neuroscience
King's College London
16 De Crespigny Park, Camberwell
London, SE5 8AB

From: freesurfer-boun...@nmr.mgh.harvard.edu 
 On Behalf Of Douglas N. Greve
Sent: 15 April 2021 15:14
To: freesurfer@nmr.mgh.harvard.edu
Subject: Re: [Freesurfer] glmfit with aseg table: view surface overlay

The input is a table, so the output is a table (the sig.mgh file will just have 
the number of values that are in the table; this is not a "volume" you can look 
at in FV). Look in the sig.table.dat file for the sigs.
On 4/13/2021 8:41 AM, Griem, Julia wrote:

External Email - Use Caution

External Email - Use Caution
Dear Freesurfer team,

I am comparing subcortical volumes (aseg table) in 2 groups with no other 
variables. Based on the GLM lecture slides from the course I attended, I ran 
the following glmfit command:
mri_glmfit
--table aseg_table.txt
--fsgd FSGD/DemographicsSubcortBinaryGroup.fsgd
--C Contrasts/HC-ASPD.mtx
--C Contrasts/ASPD-HC.mtx
--glmdir roi.binarygroup.glmdir

It created all the correct output and subdirectories.

I then used
freeview -f $SUBJECTS_DIR/fsaverage/surf/lh.inflated:overlay=ASPD-HC/sig.mgh

but an error saying "failed to load surface overlay" comes up and the terminal 
error message is "number of vertices in overlay data does not match with 
surface". I assume this is because I am trying to load a 'whole-brain' sig.mgh 
onto a lh surface? Do I have to change the glmfit command to specify 
hemispheres, and if so, how do I do that since I do not want to look at 
cortical but subcortical data? Or alternatively, can I load both hemispheres 
into freeview so the overlay sig.mgh fits?

Thank you so much,
Julia



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Re: [Freesurfer] glmfit with aseg table: view surface overlay

2021-04-15 Thread Douglas N. Greve
The input is a table, so the output is a table (the sig.mgh file will 
just have the number of values that are in the table; this is not a 
"volume" you can look at in FV). Look in the sig.table.dat file for the 
sigs.


On 4/13/2021 8:41 AM, Griem, Julia wrote:


External Email - Use Caution

External Email - Use Caution

Dear Freesurfer team,

I am comparing subcortical volumes (aseg table) in 2 groups with no 
other variables. Based on the GLM lecture slides from the course I 
attended, I ran the following glmfit command:

mri_glmfit
--table aseg_table.txt
--fsgd FSGD/DemographicsSubcortBinaryGroup.fsgd
--C Contrasts/HC-ASPD.mtx
--C Contrasts/ASPD-HC.mtx
--glmdir roi.binarygroup.glmdir

It created all the correct output and subdirectories.

I then used
freeview -f 
$SUBJECTS_DIR/fsaverage/surf/lh.inflated:overlay=ASPD-HC/sig.mgh


but an error saying "failed to load surface overlay" comes up and the 
terminal error message is "number of vertices in overlay data does not 
match with surface". I assume this is because I am trying to load a 
'whole-brain' sig.mgh onto a lh surface? Do I have to change the 
glmfit command to specify hemispheres, and if so, how do I do that 
since I do not want to look at cortical but subcortical data? Or 
alternatively, can I load both hemispheres into freeview so the 
overlay sig.mgh fits?


Thank you so much,
Julia

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[Freesurfer] glmfit with aseg table: view surface overlay

2021-04-13 Thread Griem, Julia
External Email - Use Caution

Dear Freesurfer team,

I am comparing subcortical volumes (aseg table) in 2 groups with no other 
variables. Based on the GLM lecture slides from the course I attended, I ran 
the following glmfit command:
mri_glmfit
--table aseg_table.txt
--fsgd FSGD/DemographicsSubcortBinaryGroup.fsgd
--C Contrasts/HC-ASPD.mtx
--C Contrasts/ASPD-HC.mtx
--glmdir roi.binarygroup.glmdir

It created all the correct output and subdirectories.

I then used
freeview -f $SUBJECTS_DIR/fsaverage/surf/lh.inflated:overlay=ASPD-HC/sig.mgh

but an error saying "failed to load surface overlay" comes up and the terminal 
error message is "number of vertices in overlay data does not match with 
surface". I assume this is because I am trying to load a 'whole-brain' sig.mgh 
onto a lh surface? Do I have to change the glmfit command to specify 
hemispheres, and if so, how do I do that since I do not want to look at 
cortical but subcortical data? Or alternatively, can I load both hemispheres 
into freeview so the overlay sig.mgh fits?

Thank you so much,
Julia
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