Boost Protein Expression by Codon Optimization

2004-01-14 Thread Sally Wang
Dear Colleague,

Happy New Year!

As we know, codon preference among different species could be dramatically 
different.  To enhance the expression level of a foreign protein in a 
particular expression system (E.coli, Yeast, Insect, or Mammalian cell), it is 
very important to adjust the codon frequency of the foreign protein to match 
that of the host expression system.  One classic example is GFP (green 
fluorescent protein) which was optimized to achieve high-level of expression in 
mammalian cells. 

GenScript has developed a proprietary algorithm for codon optimization.  This 
algorithm can optimize sequences for protein expression using either your own 
codon usage table or those from publicly available codon usage database.  It 
can converts your amino acid sequence into a DNA sequence with overall codon 
usage similar to a specified organism, and also optimizes the RNA secondary 
structure, GC content, repetitive codons etc. Using Genscript optimized 
synthetic genes, many of our customers have reported dramatic increase on 
protein expression. 

The optimized gene can be synthesized by GenScript gene synthesis technology 
with 100% fidelity.  The cost is as low as $2.35 per base pair.  Our service is 
very flexible, we can clone the optimized gene into our standard vectors 
(without extra charge), or any expression vector that you provide (for $400 
extra charge).

Gene synthesis is a powerful technology and it has many other applications.  
Another application example is to replace PCR Cloning.  Please visit our web 
(http://www.genscript.com/gene_synthesis.html) to learn more about this 
technology.  

Besides Gene Synthesis, we also provide custom vector-based siRNA, siRNA 
cassette, peptide, oligo, cloning and protein expression, biochemical reagents, 
and labwares.  Please visit our web site (http://www.genscript.com) to learn 
more about our services.


Sincerely,
 
Sally Wang
Account Manager
GenScript Corporation
120 Centennial Ave.
Piscataway, New Jersey 08854, USA
Tel: 1-732-885-9188, 1-732-357-3839
Fax: 1-732-210-0262
Email: [EMAIL PROTECTED]
Web: http://www.genscript.com


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WINNING NOTIFICATION/FINAL NOTICECONTACT CLAIM AGENT/ MR VAN JANSEN ON TELEPHONE +31-627-264-376

2004-01-14 Thread LOTTO INTERNATIONAL NL.
INTERNATIONAL LOTTO NL
72657, NL-2115 DB AMSTERDAM,
THE NETHERLANDS
REPLY EMAIL TO : [EMAIL PROTECTED]
From: The Promotions Manager
International Lotto/ Prize Award Department.
REF: DIAQ34761
DATE: 14th January 2004.
Attention:
14TH January 2004 DRAW RESULTS/ WINNING NOTICE
Congratulations! We announce with pleasure that you have emerged as one of
the two winners of the Lotto NL ballot draws held on 13th January 2004.
Be informed that you are among 15,000  individuals/private companies
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By our procedures, you were allocated to NL lottery ticket number
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respectively, with serial number AA5/120920. From the results of the final
draws, you have emerged as a Prime winner in the "A" category and therefore 
entitled to a winning payout amounting to Euro 1,500,000.00. (one million five 
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money has been insured and credited in your name to file Ref. No. DIAQ34761. 
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prize. Please find contact information below:
MR VAN JANSEN.
FINANCE DIRECTOR,
PREMIUM TRUST SECURITIES BV,
TEL/FAX: +31 627-264-367.
EMAIL: [EMAIL PROTECTED]
Remember, you must contact your claims officer immediately, on or before 17th 
January 2004 to avoid funds being returned as unclaimed. All correspondences to 
Aan Ross, either by fax or email, should have this email sent along with it and 
also, your email address to which this email is sent should be clearly and 
boldly written in your response. You should also include your reference number. 
Also, should there be any change of your address, do inform your claims officer 
as soon as possible.
Be advised that Lotto regulations prohibit you from publicly disclosing your 
win until you have received your prize money. This is for security reasons and 
to avoid cases of multiple claims processing. The Lotto NL holds exclusive 
right to terminate your claims processing upon any breach of this condition.
Congratulations again from all our staff and thank you for being part
of our international lotto program. It is our hope that with a part of your 
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International lotto.
Sincerely,
THE PROMOTIONS MANAGER,
LOTTO NL  

Description and patches for proposed new feature

2004-01-14 Thread Chris Ross

[EMAIL PROTECTED] wrote; in a message with the
subject "Re: How do I redraw menuitems in an active menu?":

Why don't you post your patch so that I can take a look (include
instructions to show the problem).


  Well, as it happens, I figured out the problem with
modifying the menu item.  It was just an issue of
the code not clearing the menuitem window area before
drawing a substring.  I got that fixed now, so it clears
the whole area before drawing in the case of the new
feature I added.

  Okay, included here are a description and patches for
the new keyword I added.  This keyword will allow me
to define menuitems that change based on modifier
key-presses.  The patch is based on the HEAD of the
CVS tree as of today (14-Jan-2004).

  Much feedback welcome from all!  Please, let me know
any thoughts you have!  Thanks!

   - Chris
The new ModSwitch keyword
-

  This is intended to solve a problem I've been looking to
solve.  I sometimes have menu items duplicated, with only
one minor change (as an example, ssh'ing into a remote host,
one without X port forwarding, one with.)  What I decided
the cleanest solution would be, to reduce the size of my
menu's, would be to have menuitems that would have different
labels and/or actions when you held a modifier key.

  To implement this, my first pass was the here-described
ModSwitch keyword.  When used as follows:

AddToMenu RemoteLogins  "Menu"  Title
+ "Tomcat"  ModSwitch N ( "" Exec exec xterm -e ssh tomcat ) \
S ( " w/ X fwd" Exec exec xterm -e ssh -R 
6000:127.0.0.1:6000 tomcat )
+ "Sphynx"  Exec exec xterm -e ssh sphynx

  Would cause the top [non-Title] menuitem in that menu
to ssh in without extra command line args, unless you
hold Shift, in which case it will do X forwarding
through 127.0.0.1.

  This syntax is just what I came up with.  If anyone
has any better ideas for syntax, then please convince
me.  I'd be happy to change it if a better option
presents itself.

  Caveats/ToDo's:

  - When the menu draws, the correct string isn't
displayed until you move the mouse into the menu.
I must be missing a place where I should put a
call to dink with the menuitems.
  - When you hold modifiers that don't match any
listed ones, it maintains the previous state,
instead of doing "something else".  Should there
be a concept of a "default"?  Same as N?
  - I only mess with label[0].  Why do MenuItems
even have an array of labels?  I couldn't figure
that out while I was in there...
  - I don't pre-initialize the label or action
when the config is parsed.  So, for a little while,
ModSwitch is the action.  I've got it mapped to
CMD_Beep right now, since it shouldn't really do
anything as an "action" or "command", but.  Perhaps
I should just ensure that it can never be called
in the first place by reconfiguring action and
label when it's initially scanned.
  - I'm not careful to figure out how to initialize
or not, and free if necessary, the copies of the
original label and action.
  - Right now the top-level label (before the ModSwitch
keyword) is a base, and the other string it appended
to it.  You could do "" at top, and use the new string
in each mod-switch-spec, to do whole replacement.
Should I code it to work that way instead?  This
falls back under syntax...

  Anyway.  Lots of questions, and I'd love to
hear opinions...

- Chris Ross
  [EMAIL PROTECTED]
  14-Jan-2004

Index: fvwm/commands.h
===
RCS file: /home/cvs/fvwm/fvwm/fvwm/commands.h,v
retrieving revision 1.41
diff -u -p -u -r1.41 commands.h
--- fvwm/commands.h 16 Jul 2003 09:10:40 -  1.41
+++ fvwm/commands.h 14 Jan 2004 17:32:15 -
@@ -80,6 +80,7 @@ enum
F_LAYER,
F_LOCALE_PATH,
F_MENUSTYLE,
+   F_MOD_SWITCH,
F_MODULE,
F_MODULE_PATH,
F_MODULE_SYNC,
Index: fvwm/functable.c
===
RCS file: /home/cvs/fvwm/fvwm/fvwm/functable.c,v
retrieving revision 1.29
diff -u -p -u -r1.29 functable.c
--- fvwm/functable.c16 Jul 2003 09:10:40 -  1.29
+++ fvwm/functable.c14 Jan 2004 17:32:15 -
@@ -348,6 +348,9 @@ const func_t func_table[] =
CMD_ENT("menustyle", CMD_MenuStyle, F_MENUSTYLE, 0, 0),
/* - Control appearance and behavior of a menu */
 
+   CMD_ENT("modswitch", CMD_Beep, F_MOD_SWITCH, 0, 0),
+   /* - Never called directly; sets up menu entry based on modif. keys */
+
CMD_ENT("module", CMD_Module, F_MODULE, 0, 0),
/* - Invoke an fvwm module */
 
Index: fvwm/menuitem.c
===
RCS file: /home/cvs/fvwm/fvwm/fvwm/menuitem.c,v
retrieving revision 1.32
diff -u -p -u -r1.32 menuitem.c
--- fvwm/menuitem.c 29 Jun 2003 19:53:23 -  1.32
+++ fvwm/menuitem.c 14 Jan 2004 17:

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