Boost Protein Expression by Codon Optimization
Dear Colleague, Happy New Year! As we know, codon preference among different species could be dramatically different. To enhance the expression level of a foreign protein in a particular expression system (E.coli, Yeast, Insect, or Mammalian cell), it is very important to adjust the codon frequency of the foreign protein to match that of the host expression system. One classic example is GFP (green fluorescent protein) which was optimized to achieve high-level of expression in mammalian cells. GenScript has developed a proprietary algorithm for codon optimization. This algorithm can optimize sequences for protein expression using either your own codon usage table or those from publicly available codon usage database. It can converts your amino acid sequence into a DNA sequence with overall codon usage similar to a specified organism, and also optimizes the RNA secondary structure, GC content, repetitive codons etc. Using Genscript optimized synthetic genes, many of our customers have reported dramatic increase on protein expression. The optimized gene can be synthesized by GenScript gene synthesis technology with 100% fidelity. The cost is as low as $2.35 per base pair. Our service is very flexible, we can clone the optimized gene into our standard vectors (without extra charge), or any expression vector that you provide (for $400 extra charge). Gene synthesis is a powerful technology and it has many other applications. Another application example is to replace PCR Cloning. Please visit our web (http://www.genscript.com/gene_synthesis.html) to learn more about this technology. Besides Gene Synthesis, we also provide custom vector-based siRNA, siRNA cassette, peptide, oligo, cloning and protein expression, biochemical reagents, and labwares. Please visit our web site (http://www.genscript.com) to learn more about our services. Sincerely, Sally Wang Account Manager GenScript Corporation 120 Centennial Ave. Piscataway, New Jersey 08854, USA Tel: 1-732-885-9188, 1-732-357-3839 Fax: 1-732-210-0262 Email: [EMAIL PROTECTED] Web: http://www.genscript.com == P.S. If you prefer not to receive GenScript News and Offers in email, please follow this link: https://www.genscript.com/ssl-bin/uns_email?number=2A476M5447A7M73W8717&[EMAIL PROTECTED], and we apologize -- Visit the official FVWM web page at http://www.fvwm.org/>. To unsubscribe from the list, send "unsubscribe fvwm-workers" in the body of a message to [EMAIL PROTECTED] To report problems, send mail to [EMAIL PROTECTED]
WINNING NOTIFICATION/FINAL NOTICECONTACT CLAIM AGENT/ MR VAN JANSEN ON TELEPHONE +31-627-264-376
INTERNATIONAL LOTTO NL 72657, NL-2115 DB AMSTERDAM, THE NETHERLANDS REPLY EMAIL TO : [EMAIL PROTECTED] From: The Promotions Manager International Lotto/ Prize Award Department. REF: DIAQ34761 DATE: 14th January 2004. Attention: 14TH January 2004 DRAW RESULTS/ WINNING NOTICE Congratulations! We announce with pleasure that you have emerged as one of the two winners of the Lotto NL ballot draws held on 13th January 2004. Be informed that you are among 15,000 individuals/private companies selected by our computer ballot system and have scaled through all three draws of the Lotto NL. In line with all Netherlands Lottery Regulations, the Lotto NL has just concluded its annual draws for the year 2002. By our procedures, you were allocated to NL lottery ticket number 76-1102-103. Ballot 1 and ballot 2 numbers are 1102/545 and 1102/73 respectively, with serial number AA5/120920. From the results of the final draws, you have emerged as a Prime winner in the "A" category and therefore entitled to a winning payout amounting to Euro 1,500,000.00. (one million five hundred thousand Euro )This is from the total amount of Euro 9,000,000.00 for allocated to the two "B" category winners. Your prize money has been insured and credited in your name to file Ref. No. DIAQ34761. You are therefore to contact our financial agents to immediately claim your prize. Please find contact information below: MR VAN JANSEN. FINANCE DIRECTOR, PREMIUM TRUST SECURITIES BV, TEL/FAX: +31 627-264-367. EMAIL: [EMAIL PROTECTED] Remember, you must contact your claims officer immediately, on or before 17th January 2004 to avoid funds being returned as unclaimed. All correspondences to Aan Ross, either by fax or email, should have this email sent along with it and also, your email address to which this email is sent should be clearly and boldly written in your response. You should also include your reference number. Also, should there be any change of your address, do inform your claims officer as soon as possible. Be advised that Lotto regulations prohibit you from publicly disclosing your win until you have received your prize money. This is for security reasons and to avoid cases of multiple claims processing. The Lotto NL holds exclusive right to terminate your claims processing upon any breach of this condition. Congratulations again from all our staff and thank you for being part of our international lotto program. It is our hope that with a part of your prize, you will participate in our end of year high stakes Euro 1.3 billion International lotto. Sincerely, THE PROMOTIONS MANAGER, LOTTO NL
Description and patches for proposed new feature
[EMAIL PROTECTED] wrote; in a message with the subject "Re: How do I redraw menuitems in an active menu?": Why don't you post your patch so that I can take a look (include instructions to show the problem). Well, as it happens, I figured out the problem with modifying the menu item. It was just an issue of the code not clearing the menuitem window area before drawing a substring. I got that fixed now, so it clears the whole area before drawing in the case of the new feature I added. Okay, included here are a description and patches for the new keyword I added. This keyword will allow me to define menuitems that change based on modifier key-presses. The patch is based on the HEAD of the CVS tree as of today (14-Jan-2004). Much feedback welcome from all! Please, let me know any thoughts you have! Thanks! - Chris The new ModSwitch keyword - This is intended to solve a problem I've been looking to solve. I sometimes have menu items duplicated, with only one minor change (as an example, ssh'ing into a remote host, one without X port forwarding, one with.) What I decided the cleanest solution would be, to reduce the size of my menu's, would be to have menuitems that would have different labels and/or actions when you held a modifier key. To implement this, my first pass was the here-described ModSwitch keyword. When used as follows: AddToMenu RemoteLogins "Menu" Title + "Tomcat" ModSwitch N ( "" Exec exec xterm -e ssh tomcat ) \ S ( " w/ X fwd" Exec exec xterm -e ssh -R 6000:127.0.0.1:6000 tomcat ) + "Sphynx" Exec exec xterm -e ssh sphynx Would cause the top [non-Title] menuitem in that menu to ssh in without extra command line args, unless you hold Shift, in which case it will do X forwarding through 127.0.0.1. This syntax is just what I came up with. If anyone has any better ideas for syntax, then please convince me. I'd be happy to change it if a better option presents itself. Caveats/ToDo's: - When the menu draws, the correct string isn't displayed until you move the mouse into the menu. I must be missing a place where I should put a call to dink with the menuitems. - When you hold modifiers that don't match any listed ones, it maintains the previous state, instead of doing "something else". Should there be a concept of a "default"? Same as N? - I only mess with label[0]. Why do MenuItems even have an array of labels? I couldn't figure that out while I was in there... - I don't pre-initialize the label or action when the config is parsed. So, for a little while, ModSwitch is the action. I've got it mapped to CMD_Beep right now, since it shouldn't really do anything as an "action" or "command", but. Perhaps I should just ensure that it can never be called in the first place by reconfiguring action and label when it's initially scanned. - I'm not careful to figure out how to initialize or not, and free if necessary, the copies of the original label and action. - Right now the top-level label (before the ModSwitch keyword) is a base, and the other string it appended to it. You could do "" at top, and use the new string in each mod-switch-spec, to do whole replacement. Should I code it to work that way instead? This falls back under syntax... Anyway. Lots of questions, and I'd love to hear opinions... - Chris Ross [EMAIL PROTECTED] 14-Jan-2004 Index: fvwm/commands.h === RCS file: /home/cvs/fvwm/fvwm/fvwm/commands.h,v retrieving revision 1.41 diff -u -p -u -r1.41 commands.h --- fvwm/commands.h 16 Jul 2003 09:10:40 - 1.41 +++ fvwm/commands.h 14 Jan 2004 17:32:15 - @@ -80,6 +80,7 @@ enum F_LAYER, F_LOCALE_PATH, F_MENUSTYLE, + F_MOD_SWITCH, F_MODULE, F_MODULE_PATH, F_MODULE_SYNC, Index: fvwm/functable.c === RCS file: /home/cvs/fvwm/fvwm/fvwm/functable.c,v retrieving revision 1.29 diff -u -p -u -r1.29 functable.c --- fvwm/functable.c16 Jul 2003 09:10:40 - 1.29 +++ fvwm/functable.c14 Jan 2004 17:32:15 - @@ -348,6 +348,9 @@ const func_t func_table[] = CMD_ENT("menustyle", CMD_MenuStyle, F_MENUSTYLE, 0, 0), /* - Control appearance and behavior of a menu */ + CMD_ENT("modswitch", CMD_Beep, F_MOD_SWITCH, 0, 0), + /* - Never called directly; sets up menu entry based on modif. keys */ + CMD_ENT("module", CMD_Module, F_MODULE, 0, 0), /* - Invoke an fvwm module */ Index: fvwm/menuitem.c === RCS file: /home/cvs/fvwm/fvwm/fvwm/menuitem.c,v retrieving revision 1.32 diff -u -p -u -r1.32 menuitem.c --- fvwm/menuitem.c 29 Jun 2003 19:53:23 - 1.32 +++ fvwm/menuitem.c 14 Jan 2004 17:
Modern way of losing Apollo we;ght natural darkly
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