[galaxy-dev] Database source

2012-12-02 Thread Perez, Ricardo
Hello Galaxy,

We are implementing a fresh instance of Galaxy in the school server for 
teaching purposes.  So obtaining genomic data from the UCSC servers can present 
a problem by overloading the connection.  To make things faster, we want to 
obtain the data for the genomic data and have it in our servers, speeding up 
data retrieval.  At first I was under the impression that we had to use MySQL 
for this, and downloaded MySQL files from the UCSC servers by using:

$ rsync -avzP rsync:// hgdownload.cse.ucsc.edu/mysql .

We have successfully added the data to MySQL in our server, but now do not know 
how to make galaxy see the data.  However after searching more about the 
problem, I found out that I could have used the following instead:

$ rsync -avzP rsync://datacache.g2.bx.psu.edu/indexes/ .

Was downloading the mysql files unnecessary or can I still be able to use them? 
Also, would it be more efficient to use the second rsync option instead?

Thank you for your help,
--Ricardo Perez
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Re: [galaxy-dev] The qiuck way to upload large file

2012-12-02 Thread Ianiri, Giuseppe
There is a tutorial on the home page of Galaxy (uploading data using FTP) that 
shows you step by step how to go through the uploading process.
Hope this helps 


Giuseppe Ianiri, Ph.D.



From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] 
on behalf of James Meador [jmea...@momentapharma.com]
Sent: Saturday, December 01, 2012 10:08 PM
To: galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] The qiuck way to upload large file

Hi All,
Thanks for the tip, but could you specify what directory to ftp them to? I have 
been wondering about this myself but have not found an answer yet. Also, from 
my limited testing on a local setup on Mac OS X 10.6, it appears that when you 
upload a file (170 MB fastq file in my case), it gets placed into a directory 
within /usr/local/Galaxy/database/files/000/ into a file called, 
dataset_1.dat. This file is just a text file of the original fastq file so it 
might be possible to ftp to there but I am not sure that the database or Galaxy 
will automatically be able to read this. Can anyone comment on how to ftp files 
to what directory that Galaxy will be able to add to the History?
Thanks in advance,
Jim


On Dec 1, 2012, at 8:40 AM, shamsher jagat wrote:

FTP or URL

On Sat, Dec 1, 2012 at 5:37 AM, 泽 蔡 
caizexi...@yahoo.com.cnmailto:caizexi...@yahoo.com.cn wrote:
Hi all,

I'm a new guy of galaxy and bioinformatics.
I read the document of galaxy, but it's so hard to find what I need.
I already set up a locale instance of galaxy, it works fine. The problem is I 
need to analysis large data, but the upload file is so slow, is there some 
ways I can upload large file quickly?

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Re: [galaxy-dev] pass more information on a dataset merge

2012-12-02 Thread Alex.Khassapov
Hi John,

My colleague (Neil) has a bit of a problem with the multi file support:

When I try and use the option Upload Directory of files I get the error below

Error Traceback:
View as:   Interactive  |  Text  |  XML (full)
⇝ AttributeError: 'Bunch' object has no attribute 'multifiles'
URL: http://140.253.78.218/library_common/upload_library_dataset
Module weberror.evalexception.middleware:364 in respond view
  app_iter = self.application(environ, detect_start_response)
Module paste.debug.prints:98 in __call__ view
  environ, self.app)
Module paste.wsgilib:539 in intercept_output view
  app_iter = application(environ, replacement_start_response)
Module paste.recursive:80 in __call__ view
  return self.application(environ, start_response)
Module paste.httpexceptions:632 in __call__ view
  return self.application(environ, start_response)
Module galaxy.web.framework.base:160 in __call__ view
  body = method( trans, **kwargs )
Module galaxy.web.controllers.library_common:855 in upload_library_dataset  
   view
  **kwd )
Module galaxy.web.controllers.library_common:1055 in upload_dataset view
  json_file_path = upload_common.create_paramfile( trans, uploaded_datasets )
Module galaxy.tools.actions.upload_common:342 in create_paramfile view
  multifiles = uploaded_dataset.multifiles,
AttributeError: 'Bunch' object has no attribute 'multifiles'

Any ideas? Should we check if 'multifiles' attribute is set? Or some other call 
is missing which should set it to NULL if it's missing?

-Alex

-Original Message-
From: jmchil...@gmail.com [mailto:jmchil...@gmail.com] On Behalf Of John Chilton
Sent: Wednesday, 17 October 2012 3:21 AM
To: Khassapov, Alex (CSIRO IMT, Clayton)
Subject: Re: [galaxy-dev] pass more information on a dataset merge

Wow, thanks for the rapid feedback! I have made the changes you have suggested. 
It seems you must be interested in this idea/implementation. Let me know if you 
have specific use cases/requirements in mind and/or if you would be interested 
in write access to the repository.

-John

On Mon, Oct 15, 2012 at 11:51 PM,  alex.khassa...@csiro.au wrote:
 Hi John,

 I tried your galaxy-central-homogeneous-composite-datatypes implementation, 
 works great thank you (and Jorrit).

 A couple of fixes:

 1. Add multi_upload.xml to too_conf.xml 2. 
 lib/galaxy/tools/parameters/grouping.py line 322 (in get_filenames( context 
 )) -
 if ftp_files is not None:
Remove is not None as ftp_files is empty [], but not None, then line 331 
 user_ftp_dir = os.path.join( trans.app.config.ftp_upload_dir, 
 trans.user.email ) throws an exeption if ftp_upload_dir isn't set.

 Alex

 -Original Message-
 From: galaxy-dev-boun...@lists.bx.psu.edu
 [mailto:galaxy-dev-boun...@lists.bx.psu.edu] On Behalf Of John Chilton
 Sent: Tuesday, 16 October 2012 1:07 AM
 To: Jorrit Boekel
 Cc: galaxy-dev@lists.bx.psu.edu
 Subject: Re: [galaxy-dev] pass more information on a dataset merge

 Here is an implementation of the implicit multi-file composite datatypes 
 piece of that idea. I think the implicit parallelism may be harder.

 https://bitbucket.org/galaxyp/galaxy-central-homogeneous-composite-dat
 atypes/compare

 Jorrit do you have any objection to me trying to get this included in 
 galaxy-central (this is 95% code I stole from you)? I made the changes 
 against a clean galaxy-central fork and included nothing proteomics specific 
 in anticipation of trying to do that. I have talked with Jim Johnson about 
 the idea and he believes it would be useful his mothur metagenomics tools, so 
 the idea is valuable outside of proteomics.

 Galaxy team, would you be okay with including this and if so is there 
 anything you would like to see either at a high level or at the level of the 
 actual implementation.

 -John

 
 John Chilton
 Senior Software Developer
 University of Minnesota Supercomputing Institute
 Office: 612-625-0917
 Cell: 612-226-9223
 Bitbucket: https://bitbucket.org/jmchilton
 Github: https://github.com/jmchilton
 Web: http://jmchilton.net

 On Mon, Oct 8, 2012 at 9:24 AM, John Chilton chil...@msi.umn.edu wrote:
 Jim Johnson and I have been discussing that approach to handling 
 fractionated proteomics samples as well (composite datatypes, not the 
 specifics of the interface for parallelizing).

 My perspective has been that Galaxy should be augmented with better 
 native mechanisms for grouping objects in histories, operating over 
 those groups, building workflows that involve arbitrary numbers of 
 inputs, etc... Composite data types are kindof a kludge, I think they 
 are more useful for grouping HTML files together when you don't care 
 about operating on the constituent parts you just want to view pages 
 a as a report or something. With this proteomic data we are working 
 with, the individual pieces are really interesting right? You want to 
 operate on the 

Re: [galaxy-dev] Data/history/user export and import

2012-12-02 Thread Jeremy Goecks

 I was thinking whether I could establish the same from using the DB access 
 directly!? Or would that be too dangerous? In the past I have tried to 
 transfer the user tables from an old instance into a fresh onebut failed 
 due to all sorts of unpredictable errors..

We (the Galaxy team) do not recommend that you modify the database directly; 
there's just too much going on (keys, relationships, etc) to ensure that things 
go smoothly.

In fact, the whole point of the API is to provide a high-level, programmer 
interface so that developers do not have to work at a low (e.g. database) 
level. If you were going to write some code, you'd be best to try extending the 
API to support what you want. Should you get history import/export working, 
we'd be happy to incorporate it in the Galaxy source.

Best,
J.


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[galaxy-dev] Installing dependencies

2012-12-02 Thread Franco Caramia
Hi devs,

Got the following error when trying to test the install of a dependency:

URL: 
http://bioinf-galaxy/admin_toolshed/manage_tool_dependencies?sort=-create_ti
meshow_item_checkboxes=falseasync=falsepage=1webapp=galaxyoperation=ins
tallid=00f52b22f3524111 Module paste.exceptions.errormiddleware:143 in
__call__   app_iter = self.application(environ, start_response) Module
paste.debug.prints:98 in __call__   environ, self.app) Module
paste.wsgilib:539 in intercept_output   app_iter = application(environ,
replacement_start_response) Module paste.recursive:80 in __call__   return
self.application(environ, start_response) Module paste.httpexceptions:632 in
__call__   return self.application(environ, start_response) Module
galaxy.web.framework.base:160 in __call__   body = method( trans, **kwargs
) Module galaxy.web.framework:184 in decorator   return func( self, trans,
*args, **kwargs ) Module galaxy.web.controllers.admin_toolshed:904 in
manage_tool_dependencies   self.initiate_tool_dependency_installation(
trans, tool_dependencies_for_installation ) Module galaxy.web.framework:184
in decorator   return func( self, trans, *args, **kwargs ) Module
galaxy.web.controllers.admin_toolshed:530 in
initiate_tool_dependency_installation   tool_dependencies_config =
get_config_from_disk( 'tool_dependencies.xml',
tool_shed_repository.repo_path( trans.app ) ) Module
galaxy.util.shed_util:920 in get_config_from_disk   for root, dirs, files
in os.walk( relative_install_dir ): Module os:276 in walk   names =
listdir(top) TypeError: coercing to Unicode: need string or buffer, NoneType
found

Any ideas??

Thanks,

Franco


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[galaxy-dev] Reinstalling toolshed tool

2012-12-02 Thread Franco Caramia
Hi devs,

I get the following error when trying to reinstall a deleted repository/tool
from the toolshed:

Server Error URL: 
http://bioinf-galaxy/admin_toolshed/browse_repositories?operation=activate+o
r+reinstallid=88ee2d7021e4dc1a Module paste.exceptions.errormiddleware:143
in __call__   app_iter = self.application(environ, start_response) Module
paste.debug.prints:98 in __call__   environ, self.app) Module
paste.wsgilib:539 in intercept_output   app_iter = application(environ,
replacement_start_response) Module paste.recursive:80 in __call__   return
self.application(environ, start_response) Module paste.httpexceptions:632 in
__call__   return self.application(environ, start_response) Module
galaxy.web.framework.base:160 in __call__   body = method( trans, **kwargs
) Module galaxy.web.framework:184 in decorator   return func( self, trans,
*args, **kwargs ) Module galaxy.web.controllers.admin_toolshed:350 in
browse_repositories   return self.reselect_tool_panel_section( trans,
**kwd ) Module galaxy.web.framework:184 in decorator   return func( self,
trans, *args, **kwargs ) Module galaxy.web.controllers.admin_toolshed:1352
in reselect_tool_panel_section   status=status ) Module
galaxy.web.framework:746 in fill_template   return
self.fill_template_mako( filename, **kwargs ) Module
galaxy.web.framework:757 in fill_template_mako   return template.render(
**data ) Module mako.template:296 in render   return runtime._render(self,
self.callable_, args, data) Module mako.runtime:660 in _render 
**_kwargs_for_callable(callable_, data)) Module mako.runtime:692 in
_render_context   _exec_template(inherit, lclcontext, args=args,
kwargs=kwargs) Module mako.runtime:718 in _exec_template 
callable_(context, *args, **kwargs) Module _base_mako:42 in render_body 
__M_writer(unicode(next.body())) Module
_admin_tool_shed_repository_reselect_tool_panel_section_mako:76 in
render_body   __M_writer(unicode(render_tool_dependency_section(
install_tool_dependencies_check_box, [ repo_info_dict ] ))) Module
_admin_tool_shed_repository_common_mako:204 in
render_render_tool_dependency_section   changeset_revision ) Module
posixpath:67 in join   elif path == '' or path.endswith('/'):
AttributeError: 'NoneType' object has no attribute 'endswith'

Any ideas??

Thanks,

Franco


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Confidentiality and legal privilege attached to this email 
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[galaxy-dev] status of deseq tool from main toolshed

2012-12-02 Thread Ivan Lovric
Hi guys,

Following the instructions in the README.md file from the successful
installation of the tool, I copied deseq.R to tool-data/

I've been trying to get the deseq_and_sam2counts tool on our local
galaxy-dist here:
http://toolshed.g2.bx.psu.edu/repos/nikhil-joshi/deseq_and_sam2counts
I note a prior message from Greg on certain requirements  tags that
should be added to get some things working in:
http://dev.list.galaxyproject.org/environment-variables-and-paths-for-toolshed-tools-td4656231.html
It's probably not relevant to the error that I am getting at the moment
since we've managed to install all those packages manually through R -

source(http://bioconductor.org/biocLite.R;)

biocLite(DESeq)
biocLite(aroma.light)

The following dataset was used:

dataset:

12345aba1345355777888aba24567888689


This is the error we get when running the tool. Note, it has executed the
actual tool and made use of tool-data/deseq.R

29: Diagnostic Plots for DE Seq on data
27https://galaxy-qld-dev.genome.edu.au/history
0 bytes
An error occurred running this job:*Error: Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: methods

Attaching package: ‘BiocGenerics’

The following object(s) are masked from ‘package:stats’:

xtabs

The following object(s) are*
*
*
*
*







Error: Loading required package: Biobase Loading required package:
BiocGenerics Loading required package: methods Attaching package:
‘BiocGenerics’ The following object(s) are masked from ‘package:stats’:
xtabs The following object(s) are masked from ‘package:base’:
anyDuplicated, cbind, colnames, duplicated, eval, Filter, Find, get,
intersect, lapply, Map, mapply, mget, order, paste, pmax, pmax.int, pmin,
pmin.int, Position, rbind, Reduce, rep.int, rownames, sapply, setdiff,
table, tapply, union, unique Welcome to Bioconductor Vignettes contain
introductory material; view with 'browseVignettes()'. To cite Bioconductor,
see 'citation(Biobase)', and for packages 'citation(pkgname)'. Loading
required package: locfit locfit 1.5-8 2012-04-25 Loading required package:
lattice Warning messages: 1: found methods to import for function ‘as.list’
but not the generic itself 2: found methods to import for function ‘eapply’
but not the generic itself Error in glm.fit(x = numeric(0), y = numeric(0),
weights = NULL, start = c(0.1, : object 'fit' not found Calls:
estimateDispersions ... parametricDispersionFit - glm - eval - eval -
glm.fit In addition: Warning messages: 1: In is.na(rows) : is.na() applied
to non-(list or vector) of type 'NULL' 2: In glm.fit(x = numeric(0), y =
numeric(0), weights = NULL, start = c(0.1, : no observations informative at
iteration 1


Any ideas?
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[galaxy-dev] Activate or reinstall toolshed tool

2012-12-02 Thread Franco Caramia
Hi devs, Sorry for the bad formating, here is the error I get when trying to
reinstall a toolshed tool: Module paste.exceptions.errormiddleware:143 in
__call__   app_iter = self.application(environ, start_response) Module
paste.debug.prints:98 in __call__   environ, self.app) Module
paste.wsgilib:539 in intercept_output
  app_iter = application(environ, replacement_start_response) Module
paste.recursive:80 in __call__
  return self.application(environ, start_response) Module
paste.httpexceptions:632 in __call__
  return self.application(environ, start_response) Module
galaxy.web.framework.base:160 in __call__
  body = method( trans, **kwargs ) Module galaxy.web.framework:184 in
decorator 
  return func( self, trans, *args, **kwargs ) Module
galaxy.web.controllers.admin_toolshed:350 in browse_repositories
  return self.reselect_tool_panel_section( trans, **kwd ) Module
galaxy.web.framework:184 in decorator
  return func( self, trans, *args, **kwargs ) Module
galaxy.web.controllers.admin_toolshed:1352 in reselect_tool_panel_section
  status=status ) Module galaxy.web.framework:746 in fill_template
   return self.fill_template_mako( filename, **kwargs ) Module
galaxy.web.framework:757 in fill_template_mako
  return template.render( **data ) Module mako.template:296 in render
  return runtime._render(self, self.callable_, args, data) Module
mako.runtime:660 in _render
  **_kwargs_for_callable(callable_, data)) Module mako.runtime:692 in
_render_context 
  _exec_template(inherit, lclcontext, args=args, kwargs=kwargs) Module
mako.runtime:718 in _exec_template
  callable_(context, *args, **kwargs) Module _base_mako:42 in render_body
  __M_writer(unicode(next.body())) Module
_admin_tool_shed_repository_reselect_tool_panel_section_mako:76 in render_body
  __M_writer(unicode(render_tool_dependency_section(
install_tool_dependencies_check_box, [ repo_info_dict ] ))) Module
_admin_tool_shed_repository_common_mako:204 in
render_render_tool_dependency_section
  changeset_revision ) Module posixpath:67 in join
  elif path == '' or path.endswith('/'): AttributeError: 'NoneType' object has
no attribute 'endswith'

Any ideas would be greatly appretiated.
Also, if the galaxy devs have any (as a last resort) mysql script to delete
a tool affecting all the pertinent tables it would great.

Thanks, 

Franco


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Confidentiality and legal privilege attached to this email 
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[galaxy-dev] Same error, better formatting.

2012-12-02 Thread Franco Caramia
URL:
http://bioinf-galaxy/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a
Module paste.exceptions.errormiddleware:*143* in __call__

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
app_iter *=* self*.*application*(*environ*,* start_response*)*
Module paste.debug.prints:*98* in __call__

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
environ*,* self*.*app*)*
Module paste.wsgilib:*539* in intercept_output

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
app_iter *=* application*(*environ*,* replacement_start_response*)*
Module paste.recursive:*80* in __call__

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
*return* self*.*application*(*environ*,* start_response*)*
Module paste.httpexceptions:*632* in __call__

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
*return* self*.*application*(*environ*,* start_response*)*
Module galaxy.web.framework.base:*160* in __call__

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
body *=* method*(* trans*,* kwargs *)*
Module galaxy.web.framework:*184* in decorator

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
*return* func*(* self*,* trans*,* ***args*,* kwargs *)*
Module galaxy.web.controllers.admin_toolshed:*350* in browse_repositories

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
*return* self*.*reselect_tool_panel_section*(* trans*,* kwd *)*
Module galaxy.web.framework:*184* in decorator

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
*return* func*(* self*,* trans*,* ***args*,* kwargs *)*
Module galaxy.web.controllers.admin_toolshed:*1352* in
reselect_tool_panel_section

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
status*=*status *)*
Module galaxy.web.framework:*746* in fill_template

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
*return* self*.*fill_template_mako*(* filename*,* kwargs *)*
Module galaxy.web.framework:*757* in fill_template_mako

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
*return* template*.*render*(* data *)*
Module mako.template:*296* in render

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
*return* runtime*.*_render*(*self*,* self*.*callable_*,* args*,* data*)*
Module mako.runtime:*660* in _render

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
_kwargs_for_callable*(*callable_*,* data*)**)*
Module mako.runtime:*692* in _render_context

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
_exec_template*(*inherit*,* lclcontext*,* args*=*args*,* kwargs*=*kwargs*)*
Module mako.runtime:*718* in _exec_template

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
callable_*(*context*,* ***args*,* kwargs*)*
Module _base_mako:*42* in render_body

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
__M_writer*(*unicode*(*next*.*body*(**)**)**)*
Module _admin_tool_shed_repository_reselect_tool_panel_section_mako:*76* in
render_body

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
__M_writer*(*unicode*(*render_tool_dependency_section*(*
install_tool_dependencies_check_box*,* *[* repo_info_dict *]* *)**)**)*
Module _admin_tool_shed_repository_common_mako:*204* in
render_render_tool_dependency_section

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
changeset_revision *)*
Module posixpath:*67* in join

http://bioinf-galaxy:83/admin_toolshed/browse_repositories?operation=activate+or+reinstallid=88ee2d7021e4dc1a#
*elif* path *==* '' *or* path*.*endswith*(*'/'*)**:*
*AttributeError: 'NoneType' object has no attribute 'endswith'*
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