Re: [galaxy-dev] BWA installation

2013-06-03 Thread Ross
Hi, מירב

Sounds like you might want to try reindexing and make sure you have plenty
of disk space - I have a .rbwt file from the last time I indexed using BWA
version: 0.6.2-r126 and it works fine for me.



On Sun, Jun 2, 2013 at 4:02 PM, מירב . mer...@gmail.com wrote:

 Thanks fo your answer, But when I am tring to use the bwa and to do Map
 with BWA for 
 Illuminahttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu/repos/devteam/bwa_wrappers/bwa_wrapper/1.2.3
 


 I'm getting this error:

 The alignment failed.
 Error aligning sequence. [bwa_aln] 17bp reads: max_diff = 2
 [bwa_aln] 38bp reads: max_diff = 3
 [bwa_aln] 64bp reads: max_diff = 4
 [bwa_aln] 93bp reads: max_diff = 5
 [bwa_aln] 124bp reads: max_diff = 6
 [bwa_aln] 157bp reads: max_diff = 7
 [bwa_aln] 190bp reads: max_diff = 8
 [bwa_aln] 225bp reads: max_diff = 9
 [bwt_restore_bwt] fail to open file 
 '/home/user/extra_galaxy_project/bwa/data/hg18full/hg18full.fa.rbwt'. Abort!
 Aborted (core dumped)

 It seems like the .rbwt file is missing and cause the error?!




 2013/5/30 Carl Eberhard carlfeberh...@gmail.com

 Hello, מירב

 It looks like the indexing system/format in 'bwa index' has changed
 and we haven't updated the wiki to reflect the changes.

 The reverse index (which includes the .r* files) are now part of the
 forward index. You
 should be fine to proceed.

 I'll update the wiki to reflect this.

 From https://github.com/lh3/bwa/blob/master/NEWS :

 Release 0.5.10 and 0.6.0 (12 November, 2011)
 

 The 0.6.0 release comes with two major changes. Firstly, the index data 
 structure has been changed to support genomes longer than 4GB. The forward 
 and reverse backward genome is now integrated in one index.



 On Thu, May 30, 2013 at 8:03 AM, מירב . mer...@gmail.com wrote:

 Hello all,

 I am trying to install BWA , I used the command:
   bwa index -a bwtsw /global_data/hg18full.fa  and I got only 5 files
 insted of 8
 The missing files are

- rbwt
- rpac
- .rsa

 I didn't recieve any error message while running.

 thanks for your help


 merav

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-- 
Ross Lazarus MBBS MPH;
Head, Medical Bioinformatics, BakerIDI; Tel: +61 385321444
http://scholar.google.com/citations?hl=enuser=UCUuEM4J
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[galaxy-dev] Adding a Tool for PANAMA

2013-06-03 Thread Saket Choudhary
As part of a warmup, I added a tool for perming eQTL analysis using PANAMA(
http://ml.sheffield.ac.uk/qtl/panama/).


By default PANAMA writes to stderr and creates a PANAM_results.csv file in
the same directory as the input files.

I wrote a python  wrapper
https://bitbucket.org/saketkc/galaxy-central/src/3eb26205af5a0b77fa6177d5ecd1413ffd4d7a87/tools/eqtl_tools/panama_run.py?at=gsoc2013

for this.

I however also came across how stderr text can be handled in Galaxy :
http://wiki.galaxyproject.org/Admin/Tools/ToolConfigSyntax#A.3Cstdio.3E.2C_.3Cregex.3E.2C_and_.3Cexit_code.3E_tag_sets

and thus wrote a new XML : https://gist.github.com/saketkc/5697388


Apparently the above XML runs too, but I dont get back a CSV. Essentially I
need to move the PANAMA_results.csv file to output1. Can this be done
without using a  wrapper at all , just using the XML files ?

Is there a way to nest the commands in the XML so that I could do a
move command after panama command , but how would the paths be
specified ?

Saket
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Re: [galaxy-dev] Adding a Tool for PANAMA

2013-06-03 Thread Peter Cock
On Mon, Jun 3, 2013 at 11:53 AM, Saket Choudhary sake...@gmail.com wrote:
 As part of a warmup, I added a tool for perming eQTL analysis using
 PANAMA(http://ml.sheffield.ac.uk/qtl/panama/).


 By default PANAMA writes to stderr and creates a PANAM_results.csv file in
 the same directory as the input files.

 I wrote ... XML : https://gist.github.com/saketkc/5697388

 Apparently the above XML runs too, but I dont get back a CSV. Essentially I
 need to move the PANAMA_results.csv file to output1. Can this be done
 without using a  wrapper at all , just using the XML files ?

 Is there a way to nest the commands in the XML so that I could do a
 move command after panama command , but how would the paths be specified
 ?

Hi Saket,

You could try this (two commands in one shell line), but catching error
conditions would be a problem:

commandpanama $expression_data $snp_data; mv PANAMA_results.csv
$output1/command

In cases like this where the underlying tool is too inflexible to accept an
output filename, I would personally use a wrapper script which can handle
moving things to the desired location, and raising a clear error if the file
was not created.

Peter
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Re: [galaxy-dev] Adding a Tool for PANAMA

2013-06-03 Thread Ross
Hi Saket
If you want to redirect the executable's stdout to a new Galaxy dataset
you've defined as $output, something like:
gt; '$output'
is a workable idiom for a command line AFAIK - you need to escape the
redirection '' character or you'll confuse the xml parser.
It's often possible to hack a complex command line (or use configfile to
write a script!) but as Peter says, it all boils down to personal
preference - they all work.
Use whatever you find most convenient but remember you may need to maintain
it :)

On Mon, Jun 3, 2013 at 9:05 PM, Peter Cock p.j.a.c...@googlemail.comwrote:

 On Mon, Jun 3, 2013 at 11:53 AM, Saket Choudhary sake...@gmail.com
 wrote:
  As part of a warmup, I added a tool for perming eQTL analysis using
  PANAMA(http://ml.sheffield.ac.uk/qtl/panama/).
 
 
  By default PANAMA writes to stderr and creates a PANAM_results.csv file
 in
  the same directory as the input files.
 
  I wrote ... XML : https://gist.github.com/saketkc/5697388
 
  Apparently the above XML runs too, but I dont get back a CSV.
 Essentially I
  need to move the PANAMA_results.csv file to output1. Can this be done
  without using a  wrapper at all , just using the XML files ?
 
  Is there a way to nest the commands in the XML so that I could do a
  move command after panama command , but how would the paths be
 specified
  ?

 Hi Saket,

 You could try this (two commands in one shell line), but catching error
 conditions would be a problem:

 commandpanama $expression_data $snp_data; mv PANAMA_results.csv
 $output1/command

 In cases like this where the underlying tool is too inflexible to accept an
 output filename, I would personally use a wrapper script which can handle
 moving things to the desired location, and raising a clear error if the
 file
 was not created.

 Peter


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[galaxy-dev] FW: order of menu items

2013-06-03 Thread Stephanie LE GRAS
Dear all,

We have 2 different instances of Galaxy within our institute (a test and a 
production instance).
The thing is that the two tool menus are not in the same order and we don't 
understand why because we've got the same configuration files on both instance.

Test instance
[cid:1A999B49-4E2D-4B03-A0C8-0F3E7459F730]

Production instance:
[cid:31D3C34C-1FF2-4ED9-8A0D-DF73DEE548A7]

What could explain a difference in the order of the menu items?
The thing is that I would like to get the NGS tools all together.

Thank you in advance.

Stephanie
--
Stephanie Le Gras
Bioinformatics engineer
High throughput sequencing platform
IGBMC
1, rue Laurent Fries
67404 ILLKIRCH Cedex
France
Tel. : +33 (0)3 88 65 32 73
Tel. Solexa : +33 (0)3 88 65 32 97

attachment: Capture d¹écran 2013-06-03 à 14.03.09.pngattachment: Capture d¹écran 2013-06-03 à 14.03.32.png___
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Re: [galaxy-dev] FW: order of menu items

2013-06-03 Thread Hans-Rudolf Hotz

Hi Stephanie


Try removing integrated_tool_panel.xml before you restart your galaxy 
server. There used to be a bug, where integrated_tool_panel.xml was 
not properly re-written after a restart.


Regards, Hans-Rudolf



On 06/03/2013 02:17 PM, Stephanie LE GRAS wrote:

Dear all,

We have 2 different instances of Galaxy within our institute (a test and
a production instance).
The thing is that the two tool menus are not in the same order and we
don't understand why because we've got the same configuration files on
both instance.

Test instance

Production instance:

What could explain a difference in the order of the menu items?
The thing is that I would like to get the NGS tools all together.

Thank you in advance.

Stephanie
--
Stephanie Le Gras
Bioinformatics engineer
High throughput sequencing platform
IGBMC
1, rue Laurent Fries
67404 ILLKIRCH Cedex
France
Tel. : +33 (0)3 88 65 32 73
Tel. Solexa : +33 (0)3 88 65 32 97



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Re: [galaxy-dev] FW: order of menu items

2013-06-03 Thread Stephanie LE GRAS
Thank you very much for your very fast answer!!

It's worked!


Stephanie

Le 03/06/13 14:36, « Hans-Rudolf Hotz » h...@fmi.ch a écrit :

Hi Stephanie


Try removing integrated_tool_panel.xml before you restart your galaxy
server. There used to be a bug, where integrated_tool_panel.xml was
not properly re-written after a restart.

Regards, Hans-Rudolf



On 06/03/2013 02:17 PM, Stephanie LE GRAS wrote:
 Dear all,

 We have 2 different instances of Galaxy within our institute (a test and
 a production instance).
 The thing is that the two tool menus are not in the same order and we
 don't understand why because we've got the same configuration files on
 both instance.

 Test instance

 Production instance:

 What could explain a difference in the order of the menu items?
 The thing is that I would like to get the NGS tools all together.

 Thank you in advance.

 Stephanie
 --
 Stephanie Le Gras
 Bioinformatics engineer
 High throughput sequencing platform
 IGBMC
 1, rue Laurent Fries
 67404 ILLKIRCH Cedex
 France
 Tel. : +33 (0)3 88 65 32 73
 Tel. Solexa : +33 (0)3 88 65 32 97



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[galaxy-dev] Problem with tool in R

2013-06-03 Thread Ramon Tiburski
I have a problem with a tool in R. I can't load the RPostgreSQL library that I 
get the following error:

ERROR: carregando pacotes exigidos: dbi

My xml is this:

command interpreter=bashr_wrapper.sh $script_file/command

configfiles
configfile name=script_file
library(RPostgreSQL)
...
/configfile
/configfiles

I have this library installed at my system, what can I do?
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[galaxy-dev] Tool Visibility

2013-06-03 Thread Michael E. Cotterell
Suppose a tool didn't have any kind of configuration and therefore didn't need 
to be displayed in the Analyze Data part of Galaxy. Is there a way to modify 
tool_conf.xml so that a particular tool is only displayed in the Workflow 
editor? 

Thanks! 

Sincerely,
Michael E. Cotterell

Ph.D. Student in Computer Science, University of Georgia
Instructor of Record, Graduate RA  TA, University of Georgia
mepcotter...@gmail.com (mailto:mepcotter...@gmail.com)
mepc...@uga.edu (mailto:mepc...@uga.edu)
m...@cs.uga.edu (mailto:m...@cs.uga.edu)
http://michaelcotterell.com/


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Re: [galaxy-dev] Run galaxy on a specific SGE queue

2013-06-03 Thread Sarah Maman

Thanks Nate,

In fact,  our ~/.sge_request  set workq by default.
So, we have changed this parameter in  ~/.sge_request file in order to 
change the working queue.


Thanks again for your help,
Sarah




Nate Coraor a écrit :

On May 22, 2013, at 11:36 AM, Sarah Maman wrote:

  

Hi everyone,
Galaxy is set up on our cluster, and so far everything is working just fine. 
However, we'd like to change the submission queue from the default to a 
specific one.

To do so, we tryed to set on the universe_wsgi.ini file the 
default_cluster_job_runner parameter as following:
default_cluster_job_runner = drmaa://-q test.q -l mem=8G -l h_vmem=10G/

When doing so, an error occured telling us the job has been rejected because 2 
queues were specified: the workq (default one) and the test.q.
Should be the queue setting this way ? What are we doing wrong ?



Hi Sarah,

Without knowing what's going on in your environment I can't say for sure, but I 
would guess that something else is (~/.sge_request?) is also attempting to set 
the queue.  Are you able to use `qsub -q test.q` on the command line as the 
same user that runs Galaxy?

Thanks,
--nate

  

thanks for your help,
Sarah
Sarah Maman
INRA - LGC - SIGENAE
Chemin de Borde-Rouge - Auzeville - BP 52627
31326 Castanet-Tolosan cedex - FRANCE
Tel:   +33(0)5.61.28.57.08 
Fax:   +33(0)5.61.28.57.53

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--
 --*--
Sarah Maman
INRA - LGC - SIGENAE
http://www.sigenae.org/
Chemin de Borde-Rouge - Auzeville - BP 52627
31326 Castanet-Tolosan cedex - FRANCE
Tel:   +33(0)5.61.28.57.08
Fax:   +33(0)5.61.28.57.53 
--*--


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Re: [galaxy-dev] login to ftp server failed or Problem with ftp connexion to Galaxy

2013-06-03 Thread Danni Yu
Dear Björn,

Yes, this helps so much! Thanks a lot!

Sincerely,

Danni




On May 31, 2013, at 3:34 PM, Björn Grüning wrote:

 Hi Danni,
 
 the following is copied from a Mail from Jennifer Jackson 2 days ago.
 For some reason this mail is not indexed from google, yet.
 
 Hope that helps,
 Björn
 
 
 Dear Galaxy Main Community,
 
 We apologize for recent FTP login issues, but are glad to report that a
 solution is now in place.
 
 May 29th 2013 FTP login resolution
 
 Issue: FTP login failed for user accounts newly created or that had
 passwords 
 updated during the time frame May 20th - May 29th.
 
 Solution: Change your Galaxy password using the form on the Main
 instance 
 at http://usegalaxy.org
 
 User - Preferences - Manage your information
 
 On the Manage User Information form, change your password
 
 Wait ~20 minutes for the server to update, then use FTP with the new
 credentials
 How to use FTP Upload
 
 Share notice: http://bit.ly/ftpmay29
 
 
 Thanks for using Galaxy,
 
 The Galaxy Team
 ---
 
 To whom this may concern, 
 
 This is Danni Yu, a Galaxy user starting to use Galaxy since 2011. I was 
 able to upload large files through ftp in 2011 and 2012. Now, I am facing 
 the same problem as the other users (such as Barbara Sixt on May 27, 2013 
 and Fabrice Besnard on May 22, 2013). The error message is 530 login 
 incorrect.
 
 Ms. Jennifer Hillman-Jackson in Galaxy Support and Training team very kindly 
 mentioned that Please feel free to contact us for an update if this is 
 still a problem by next Friday on May 24, 2013. 
 
 Since it is already Friday but the problem still exists, I am hoping to 
 learn whether a solution has been provided. 
 
 Thanks very much and Sincerely,
 
 Danni
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Re: [galaxy-dev] [galaxy-user] List of genomes

2013-06-03 Thread YBao
Hi All,

Can some one tell me the parameters used in the Galaxy Mapping
Illumina with Bowtie defined as the common settings? I was trying to
replicate the results from Galaxy using my own desktop installation of
Bowtie but they differ by a rather big margin.

Thanks much,

Yongde


On Mon, Apr 29, 2013 at 1:06 PM, Jennifer Jackson j...@bx.psu.edu wrote:

  Hello Yongde,

 The list of genomes is gathered from many sources and is comprehensive to
 facilitate external display functionality (at UCSC - main and microbial-,
 Ensembl, Wormbase, etc.). When assigning a dataset in a standard format to
 one of these sources, those available will appear as links within the
 dataset's box.

 Trackster (Galaxy's native visualization tool) is available to most common
 data formats, even in the absence of an assigned database, through the use
 of the Custom Reference Genome function (aka Custom Build). We think this
 is a great advantage, in particular for cases such as yours - since you
 don't have restrict yourself to external applications that happen to host
 your genome. Click on the link here and select Trackster to give it a
 test run:


 The Custom Reference Genome function is also intended to be used for
 smaller genomes such as this one when performing alignments and most other
 jobs - no pre-indexing of the genome is necessary. Simply load the genome
 in fasta format as a dataset and use it with tools, using a reference
 genome from the history. The rational is that these are many, small,
 easily indexed during the course of job processing, and provides immediate
 access to genomes that are either newly published, or not widely used, or
 simply too numerous as a whole class for us to practically process in full
 and keep current.

 We have detailed help about how to use the Custom Reference Genome method,
 including troubleshooting help should you need it, although in practice you
 will likely find this to be fairly simple with 2-3 preparatory steps,
 depending on the source. Most if not all of these can be done within Galaxy.
 http://wiki.galaxyproject.org/Support#Custom_reference_genome

 Hopefully this helps. If you do need more guidance, please let us know,

 Best,

 Jen
 Galaxy team


 On 4/29/13 9:01 AM, YBao wrote:

 Hi All,

  I was trying to map a set of data to a genome, Klebsiella pneumoniae
 subsp. Pneumoniae MGH 78578(31). While uploading the reads, I was able to
 find the reference genome as listed above. However, when I tried to map the
 data using wither bowtie or BWA, the pull down list did not include this
 genome. Can someone help or enlighten me as why it did not make into the
 list?

  Thanks

  Yongde

  --
 Yongde Bao
 DNA Sciences Core
 Dept. of Microbiology, Immunology,
  and Cancer Biology
 UVA


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 --
 Jennifer Hillman-Jackson
 Galaxy Support and Traininghttp://galaxyproject.org




-- 
Yongde Bao
DNA Sciences Core
Dept. of Microbiology, Immunology,
 and Cancer Biology
UVA
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[galaxy-dev] storage utilisation on the main galaxy instance

2013-06-03 Thread Nikolay N.
Hi,

I had storage utilisation of +60% and two days ago I decided to remove a
large chunk of my data. I did this by clicking on the delete links for
each of these datasets. Today I've logged in and see that my storage
utilisation hasn't changed. Is there anything else that I need to do to
free up the storage?

many thanks,
Nick
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[galaxy-dev] Stable usegalaxy.org links for main Tool Shed repositories?

2013-06-03 Thread Peter Cock
Hello all,

Having asked this before, I knew the friendly and memorable alias
http://usegalaxy.org/toolshed/ existed for the main Galaxy Tool Shed,
http://toolshed.g2.bx.psu.edu

Currently tools there have a unique stable shareable URL like this:
http://toolshed.g2.bx.psu.edu/view/devteam/ncbi_blast_plus

(Due to the current frame based design, that redirects to a
less pleasant URL)

From a little experimentation, the following style aliases also work:
http://usegalaxy.org/toolshed/view/devteam/ncbi_blast_plus

Is that deliberate? Can it be relied on, and are these style URLs
actually preference? If so, could the Tool Shed be enhanced
to allow a default stem for the URL (rather than the current
host name) to show these URLs instead?

(Personally I find the usegalaxy.org based URLs more presentable
compared to toolshed.g2.bx.psu.edu which feels less permanent.)

Thanks,

Peter
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Re: [galaxy-dev] Information for Galaxy Event Horizon

2013-06-03 Thread Dave Clements
Hi Sarah,

We'd be happy to put this on the Galaxy Event Horizon page on the wiki.
 Since the event is over, we would list it in the past events section.

Is there a web page for the workshop or slides that we can link to?

And, for future reference, wiki update requests can be sent directly
to Galaxy Outreach outre...@galaxyproject.org.

Thanks,

Dave C.


On Thu, May 30, 2013 at 7:18 AM, Sarah Maman
sarah.ma...@toulouse.inra.frwrote:

 **

 Dear all,


  Four days of training in genomics are occurring on our local instance of
 Galaxy (27-30 May).

 This training session takes place at INRA, Toulouse Auzeville, France, as
 part of a collaboration between Sigenae (http://www.sigenae.org/) and
 GenoToul bioinformatics platform (http://bioinfo.genotoul.fr/).

 This session is composed of four training days:

-

Galaxy first step


-

Reads alignment and SNP calling


-

RNAseq alignment and transcripts assemblies


-

sRNAseq



 Do you think it is possible to list our training session in your Galaxy
 Event Horizon?

 Sincerely,

 Sarah Maman



  --
   --*--
 Sarah Maman
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[galaxy-dev] mapping Summary from Bowtie

2013-06-03 Thread sridhar srinivasan
Dear Developers,

I am running Bowtie2 through galaxy interface, previously it gives the the
mapping summary and convert the format to sam.. but now it directly convert
the fastq file to bam and doesnt give the mapping summary.

how to get the mapping summary from bowtie2 using galaxy interface..

Thanks
Sridhar
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