[galaxy-dev] Bowtie2 error: Creates a 62 kb file.

2013-07-24 Thread Perez, Ricardo
Dear all,

Today we ran some problems when running bowtie2.  When we run bowtie2, the run 
manages to complete.  However, bowtie2 creates a 62kb file.
The error log that we get is the following:

galaxy.jobs DEBUG 2013-07-23 17:09:42,378 (1483) Persisting job destination 
(destination id: local:///)
galaxy.jobs.handler INFO 2013-07-23 17:09:42,392 (1483) Job dispatched
galaxy.tools DEBUG 2013-07-23 17:09:42,556 Building dependency shell command 
for dependency 'bowtie2'
galaxy.tools WARNING 2013-07-23 17:09:42,556 Failed to resolve dependency on 
'bowtie2', ignoring
galaxy.tools DEBUG 2013-07-23 17:09:42,556 Building dependency shell command 
for dependency 'samtools'
galaxy.tools WARNING 2013-07-23 17:09:42,556 Failed to resolve dependency on 
'samtools', ignoring
galaxy.jobs.runners.local DEBUG 2013-07-23 17:09:42,626 (1483) executing: 
bowtie2 --version  
/usr/local/galaxy/galaxy-dist/database/tmp/GALAXY_VERSION_STRING_1483; python 
/usr/local/galaxy/galaxy-dist/tools/sr_mapping/bowtie2_wrapper.py   
   --num-threads=4  
--output=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1673.dat  
 
--indexes-path=/data/db/fungidb/Ncrassa_OR74A/bowtie2/Ncrassa_wt_Chr01.Chr07.+ChrM.+rDNA_v12
  --single-paired=paired  
--input1=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1668.dat  

--input2=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1669.dat  
   -I 0 -X 250  --settings=preSet
galaxy.jobs DEBUG 2013-07-23 17:09:42,696 (1483) Persisting job destination 
(destination id: local:///)
galaxy.jobs.runners.local DEBUG 2013-07-23 17:09:42,766 execution finished: 
bowtie2 --version  
/usr/local/galaxy/galaxy-dist/database/tmp/GALAXY_VERSION_STRING_1483; python 
/usr/local/galaxy/galaxy-dist/tools/sr_mapping/bowtie2_wrapper.py   
   --num-threads=4  
--output=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1673.dat  
 
--indexes-path=/data/db/fungidb/Ncrassa_OR74A/bowtie2/Ncrassa_wt_Chr01.Chr07.+ChrM.+rDNA_v12
  --single-paired=paired  
--input1=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1668.dat  

--input2=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1669.dat  
   -I 0 -X 250  --settings=preSet
galaxy.jobs.runners DEBUG 2013-07-23 17:09:42,850 executing external set_meta 
script for job 1483: /usr/local/galaxy/galaxy-dist/set_metadata.sh 
./database/files 
/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483 . 
/usr/local/galaxy/galaxy-dist/universe_wsgi.ini 
/usr/local/galaxy/galaxy-dist/database/tmp/tmpUiqpPD 
/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483/galaxy.json
 
/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483/metadata_in_HistoryDatasetAssociation_1767_wqhEmr,/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483/metadata_kwds_HistoryDatasetAssociation_1767_qVZjpG,/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483/metadata_out_HistoryDatasetAssociation_1767_vIfpRO,/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483/metadata_results_HistoryDatasetAssociation_1767_UNl6OR,,/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483/metadata_over!
 ride_HistoryDatasetAssociation_1767_MZilrL
165.91.11.135 - - [23/Jul/2013:17:09:43 -0500] GET 
/api/histories/ca8efa3c47500414 HTTP/1.1 200 - 
http://galaxy.tamu.edu/history; Mozilla/5.0 (X11; Linux x86_64) 
AppleWebKit/537.22 (KHTML, like Gecko) Ubuntu Chromium/25.0.1364.160 
Chrome/25.0.1364.160 Safari/537.22
165.91.11.231 - - [23/Jul/2013:17:09:43 -0500] GET /history HTTP/1.1 200 - 
http://galaxy.tamu.edu/tool_runner/index; Mozilla/5.0 (Macintosh; Intel Mac 
OS X 10_6_8) AppleWebKit/537.36 (KHTML, like Gecko) Chrome/28.0.1500.71 
Safari/537.36
165.91.11.231 - - [23/Jul/2013:17:09:43 -0500] GET 
/api/histories/1ab12f1e464820dd HTTP/1.1 200 - 
http://galaxy.tamu.edu/history; Mozilla/5.0 (Macintosh; Intel Mac OS X 
10_6_8) AppleWebKit/537.36 (KHTML, like Gecko) Chrome/28.0.1500.71 
Safari/537.36
165.91.11.231 - - [23/Jul/2013:17:09:43 -0500] GET 
/history/get_display_application_links HTTP/1.1 200 - 
http://galaxy.tamu.edu/history; Mozilla/5.0 (Macintosh; Intel Mac OS X 
10_6_8) AppleWebKit/537.36 (KHTML, like Gecko) Chrome/28.0.1500.71 
Safari/537.36
galaxy.jobs.runners DEBUG 2013-07-23 17:09:44,457 execution of external 
set_meta for job 1483 finished
galaxy.datatypes.metadata DEBUG 2013-07-23 17:09:44,545 loading metadata from 
file for: HistoryDatasetAssociation 1767

We currently have the most recent version of bowtie2 and samtools.  Anyone know 
how to fix this?

Thank you for your time,
--Ricardo Perez

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[galaxy-dev] GATK fasta file missing ERROR

2013-07-24 Thread Sridhar Srinivasan
Dear Developers,

I am GATK (beta ) Unified Genotyper module to get the indels and SNP.

I placed the jar file in (/galaxy-dist/tool-data/shared/jars/gatk) and i
link the reference genome in .loc file (gatk_sorted_picard_index.loc). I
could see the Name i mentioned in the .loc file is appearing in the galaxy
page while choosing the Reference genome.

Now while running GATK in galaxy , The command runs for a while and gives
ERROR as  i am getting ERROR as *(The fasta file you specified
(/tmp/tmp-gatk-Jjg9Mi) does not exist.)*

Could anybody suggest on the same..


Thanks
Sridhar
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[galaxy-dev] tool input is folder rather than file

2013-07-24 Thread Pankaj Narang
Dear Developers,

I want to design a tool whose input is folder containing files and
sub-folders.
I was wandering which value should I assign to TYPE attribute of PARAM
tag in INPUTS.
-- 
Best regards,
Pankaj Narang
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[galaxy-dev] Disable cheetah filtering

2013-07-24 Thread Joachim Jacob | VIB |

Hi all,


A Cheetah question: when I enter ; as a value in a text box of a tool 
(stored in $exp), Cheetah prints this as X, and passed this string to 
the tool.


How to disable this behaviour in Cheetah? I have tried to play with 
#filter directives, but to no avail.



Thanks,
Joachim

--
Joachim Jacob
Contact details: http://www.bits.vib.be/index.php/about/80-team


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Re: [galaxy-dev] Disable cheetah filtering

2013-07-24 Thread Björn Grüning
Hi Joachim,

try that one:
http://wiki.galaxyproject.org/Admin/Tools/ToolConfigSyntax#A.3Csanitizer.3E_tag_set

Cheers,
Bjoern

 Hi all,
 
 
 A Cheetah question: when I enter ; as a value in a text box of a tool 
 (stored in $exp), Cheetah prints this as X, and passed this string to 
 the tool.
 
 How to disable this behaviour in Cheetah? I have tried to play with 
 #filter directives, but to no avail.
 
 
 Thanks,
 Joachim
 



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Re: [galaxy-dev] Disable cheetah filtering

2013-07-24 Thread Peter Cock
On Wed, Jul 24, 2013 at 9:40 AM, Joachim Jacob | VIB |
joachim.ja...@vib.be wrote:
 Hi all,


 A Cheetah question: when I enter ; as a value in a text box of a tool
 (stored in $exp), Cheetah prints this as X, and passed this string to the
 tool.

 How to disable this behaviour in Cheetah? I have tried to play with #filter
 directives, but to no avail.


 Thanks,
 Joachim

That sounds like the Galaxy character sanitation making the change,
things like pipes etc can have unexpected results in command lines.
Have a look at the sanitizer tag for use in the tool's XML file.

Peter
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[galaxy-dev] All processes are freezed

2013-07-24 Thread Ali Sharifi Zarchi
Hi everybody

Since yesterday all processes I have run on Galaxy main are waiting. The
system displayed a message to contact a Galaxy admin, but I could find no
other way rather than this mailing list.

Is this freeze due to a public maintenance, or it's just my problem?

Best wishes,
Ali
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Re: [galaxy-dev] Disable cheetah filtering

2013-07-24 Thread Joachim Jacob | VIB |
Thanks both of you! Okay, so I was looking in the wrong direction. This 
solved the issue.



Cheers,
Joachim

Joachim Jacob
Contact details: http://www.bits.vib.be/index.php/about/80-team


On 07/24/2013 11:13 AM, Björn Grüning wrote:

Hi Joachim,

try that one:
http://wiki.galaxyproject.org/Admin/Tools/ToolConfigSyntax#A.3Csanitizer.3E_tag_set

Cheers,
Bjoern


Hi all,


A Cheetah question: when I enter ; as a value in a text box of a tool
(stored in $exp), Cheetah prints this as X, and passed this string to
the tool.

How to disable this behaviour in Cheetah? I have tried to play with
#filter directives, but to no avail.


Thanks,
Joachim








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Re: [galaxy-dev] Disable cheetah filtering

2013-07-24 Thread Joachim Jacob | VIB |
Okay, normally I would have forked the galaxy-dist and do a pull request 
with my change.


But the change I want to commit is in the tool 'add_value', which is in 
the toolshed. Seems like a safer approach to me to contribute to the 
toolshed. How can I commit my changes to that tool? Can somebody of the 
devteam grant me acces? Do I send the diff by email :-) ?



Cheers,
Joachim


Joachim Jacob
Contact details: http://www.bits.vib.be/index.php/about/80-team


On 07/24/2013 11:17 AM, Peter Cock wrote:

On Wed, Jul 24, 2013 at 9:40 AM, Joachim Jacob | VIB |
joachim.ja...@vib.be wrote:

Hi all,


A Cheetah question: when I enter ; as a value in a text box of a tool
(stored in $exp), Cheetah prints this as X, and passed this string to the
tool.

How to disable this behaviour in Cheetah? I have tried to play with #filter
directives, but to no avail.


Thanks,
Joachim

That sounds like the Galaxy character sanitation making the change,
things like pipes etc can have unexpected results in command lines.
Have a look at the sanitizer tag for use in the tool's XML file.

Peter




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Re: [galaxy-dev] Disable cheetah filtering

2013-07-24 Thread Björn Grüning
Hi,

 Okay, normally I would have forked the galaxy-dist and do a pull request 
 with my change.
 
 But the change I want to commit is in the tool 'add_value', which is in 
 the toolshed. Seems like a safer approach to me to contribute to the 
 toolshed. How can I commit my changes to that tool? Can somebody of the 
 devteam grant me acces? Do I send the diff by email :-) ?

Yes, please send the patch to the mailing list and/or the devteam
(toolshed should have a contact button). Also try to fill a trello
card :)

Thanks!
Bjoern

 
 Cheers,
 Joachim
 
 
 Joachim Jacob
 Contact details: http://www.bits.vib.be/index.php/about/80-team
 
 
 On 07/24/2013 11:17 AM, Peter Cock wrote:
  On Wed, Jul 24, 2013 at 9:40 AM, Joachim Jacob | VIB |
  joachim.ja...@vib.be wrote:
  Hi all,
 
 
  A Cheetah question: when I enter ; as a value in a text box of a tool
  (stored in $exp), Cheetah prints this as X, and passed this string to the
  tool.
 
  How to disable this behaviour in Cheetah? I have tried to play with #filter
  directives, but to no avail.
 
 
  Thanks,
  Joachim
  That sounds like the Galaxy character sanitation making the change,
  things like pipes etc can have unexpected results in command lines.
  Have a look at the sanitizer tag for use in the tool's XML file.
 
  Peter
 
 
 



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Re: [galaxy-dev] Disable cheetah filtering

2013-07-24 Thread Peter Cock
On Wed, Jul 24, 2013 at 11:25 AM, Björn Grüning
bjoern.gruen...@pharmazie.uni-freiburg.de wrote:
 Hi,

 Okay, normally I would have forked the galaxy-dist and do a pull request
 with my change.

 But the change I want to commit is in the tool 'add_value', which is in
 the toolshed. Seems like a safer approach to me to contribute to the
 toolshed. How can I commit my changes to that tool? Can somebody of the
 devteam grant me acces? Do I send the diff by email :-) ?

 Yes, please send the patch to the mailing list and/or the devteam
 (toolshed should have a contact button). Also try to fill a trello
 card :)

 Thanks!
 Bjoern

In general the upstream repository for a given tool will depend on
the tool author's preferences. They may not even use a separate
repository and work directly with the ToolShed in some cases.
In other cases you might be able to contribute directly via a
github pull request (planned for the NCBI BLAST+ tools), or
a bitbucket pull request (the Galaxy team like bitbucket).

The 'add_value' tool is under 'devteam' and it was original in
the main Galaxy repository under BitBucket - I don't know
where it lives now outside the tool shed:
http://toolshed.g2.bx.psu.edu/view/devteam/add_value

Regards,

Peter

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Re: [galaxy-dev] Disable cheetah filtering

2013-07-24 Thread Joachim Jacob | VIB |
Hmm, seems that collaborating on tools is kind of a burden... Three 
places to notify this small change you say? (trello, galaxy-dev and 
direct emailing to the authors.)


I have opted for Trello, card #1013
Cheers,
J

Joachim Jacob
Contact details: http://www.bits.vib.be/index.php/about/80-team


On 07/24/2013 12:45 PM, Peter Cock wrote:

On Wed, Jul 24, 2013 at 11:25 AM, Björn Grüning
bjoern.gruen...@pharmazie.uni-freiburg.de wrote:

Hi,


Okay, normally I would have forked the galaxy-dist and do a pull request
with my change.

But the change I want to commit is in the tool 'add_value', which is in
the toolshed. Seems like a safer approach to me to contribute to the
toolshed. How can I commit my changes to that tool? Can somebody of the
devteam grant me acces? Do I send the diff by email :-) ?

Yes, please send the patch to the mailing list and/or the devteam
(toolshed should have a contact button). Also try to fill a trello
card :)

Thanks!
Bjoern

In general the upstream repository for a given tool will depend on
the tool author's preferences. They may not even use a separate
repository and work directly with the ToolShed in some cases.
In other cases you might be able to contribute directly via a
github pull request (planned for the NCBI BLAST+ tools), or
a bitbucket pull request (the Galaxy team like bitbucket).

The 'add_value' tool is under 'devteam' and it was original in
the main Galaxy repository under BitBucket - I don't know
where it lives now outside the tool shed:
http://toolshed.g2.bx.psu.edu/view/devteam/add_value

Regards,

Peter




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Re: [galaxy-dev] Disable cheetah filtering

2013-07-24 Thread Peter Cock
On Wed, Jul 24, 2013 at 1:56 PM, Joachim Jacob | VIB |
joachim.ja...@vib.be wrote:
 Hmm, seems that collaborating on tools is kind of a burden... Three places
 to notify this small change you say? (trello, galaxy-dev and direct emailing
 to the authors.)

 I have opted for Trello, card #1013
 Cheers,

https://trello.com/c/GVq8Vpsi/1013-bug-some-characters-not-permitted-in-add-column-tool

Well in this case the tool authors are the Galaxy Developers, so you
can email them directly, via the galaxy-dev list, or their issue tracker
which is Trello.

Ideally every tool's README file would include contact details...

Peter
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Re: [galaxy-dev] Test ToolShed repository_dependencies.xml processing

2013-07-24 Thread Dave Bouvier

Peter,

Thank you for reporting this issue, it has been fixed in 
10253:27b458743a11, and the test tool shed has been updated to that 
revision.


   --Dave B.

On 7/23/13 12:29:34.000, Peter Cock wrote:

On Tue, Jul 23, 2013 at 5:20 PM, Peter Cock p.j.a.c...@googlemail.com wrote:

Hi Gred,

I'm trying to update this repository (initially just to switch my
README file to reStructuredText as the current plain text is
not being rendered nicely):
http://testtoolshed.g2.bx.psu.edu/view/peterjc/get_orfs_or_cdss

Uploading my new tar-ball failed:

Internal Server Error
Galaxy was unable to sucessfully complete your request

URL: 
http://testtoolshed.g2.bx.psu.edu/upload/upload?repository_id=8067bb858e9138ab
...


Same again for http://testtoolshed.g2.bx.psu.edu/view/peterjc/seq_rename
which uses the same repository_dependencies.xml file.

Peter
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Re: [galaxy-dev] [galaxy-iuc] [patch] enabling rst rendered readme file in TS

2013-07-24 Thread Greg Von Kuster
Hi peter,

I've made this change in 10254:e22f360cda13, so now only those readme files 
whose file anme ends in .rst will be rendered as such.

Thanks!

Greg Von kuster

On Jul 23, 2013, at 12:24 PM, Peter Cock p.j.a.c...@googlemail.com wrote:

 On Fri, Jul 12, 2013 at 3:35 AM, Greg Von Kuster g...@bx.psu.edu wrote:
 Hello Björn,
 
 This one has been applied in changeset 10219:a7edac403b6c, and all of
 your patches are now running on the test tool shed.
 
 Thanks very much,
 
 Greg Von Kuster
 
 Hi Greg  Björn,
 
 I think supporting reStructuredText is a good idea, but should not be
 done automatically, consider:
 
 http://testtoolshed.g2.bx.psu.edu/view/peterjc/get_orfs_or_cdss/26ce6e046040
 
 This README file is named with the extension *.txt and does not render
 nicely if treated as *.rst, things like System Message: ERROR and
 System Message: WARNING are shown to the user.
 
 I suggest that the ToolShed only render like this if the README file
 is using the *.rst extension, but otherwise default to plain text (e.g.
 no extension or *.txt files).
 
 Thanks,
 
 Peter
 


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Re: [galaxy-dev] workflow: default parameters

2013-07-24 Thread Keilwagen, Jens
Hi James,

thanks for the short reply.

I have created a trello card. Here is the link 
https://trello.com/c/SRxD991Y/1012-workflow-default-parameters
Feel free to vote.

Best regards, Jens

 -Ursprüngliche Nachricht-
 Von: ja...@taylorlab.org [mailto:ja...@taylorlab.org] Im Auftrag von
 James Taylor
 Gesendet: Montag, 22. Juli 2013 19:28
 An: Keilwagen, Jens
 Cc: galaxy-...@bx.psu.edu
 Betreff: Re: [galaxy-dev] workflow: default parameters
 
 Not currently possible, but it shouldn't be too difficult to make work.
 Please file a Trello card.
 
 On Tue, Jul 9, 2013 at 11:36 AM, Keilwagen, Jens
 jens.keilwa...@jki.bund.de wrote:
  when building workflows we can choose for each parameter whether to
 'set at runtime' or to 'set in advance' (when creating or editing the
 workflow). We are looking for a possiblity to set a default value that
 is used (only) for the workflow, i.e., we like to specify a value that
 might be used by most users, but might be altered by expert users. This
 value might be different from the default value of the corresponding
 tool. So far, we did not find any possiblity.
 
 
 --
 James Taylor, Assistant Professor, Biology/CS, Emory University

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Re: [galaxy-dev] [galaxy-iuc] [patch] enabling rst rendered readme file in TS

2013-07-24 Thread Peter Cock
On Tue, Jul 23, 2013 at 5:24 PM, Peter Cock p.j.a.c...@googlemail.com wrote:
 On Fri, Jul 12, 2013 at 3:35 AM, Greg Von Kuster g...@bx.psu.edu wrote:
 Hello Björn,

 This one has been applied in changeset 10219:a7edac403b6c, and all of
 your patches are now running on the test tool shed.

 Thanks very much,

 Greg Von Kuster

 Hi Greg  Björn,

 I think supporting reStructuredText is a good idea, but should not be
 done automatically, consider:

 http://testtoolshed.g2.bx.psu.edu/view/peterjc/get_orfs_or_cdss/26ce6e046040

 This README file is named with the extension *.txt and does not render
 nicely if treated as *.rst, things like System Message: ERROR and
 System Message: WARNING are shown to the user.

 I suggest that the ToolShed only render like this if the README file
 is using the *.rst extension, but otherwise default to plain text (e.g.
 no extension or *.txt files).

 Thanks,

 Peter

Thanks Greg for doing that:
https://bitbucket.org/galaxy/galaxy-central/commits/e22f360cda1363d0c93a86d64c17032e7507b296

Here's a follow-up patch to hopefully fix the rendering of these revisions
where I have switched to an *.rst file but the filename isn't used:
http://testtoolshed.g2.bx.psu.edu/view/peterjc/seq_rename/a119cd123b2d
http://testtoolshed.g2.bx.psu.edu/view/peterjc/get_orfs_or_cdss/a119cd123b2d

Thanks,

Peter

$ hg diff
diff -r e22f360cda13 lib/tool_shed/util/metadata_util.py
--- a/lib/tool_shed/util/metadata_util.py   Wed Jul 24 10:34:21 2013 -0400
+++ b/lib/tool_shed/util/metadata_util.py   Wed Jul 24 15:39:44 2013 +0100
@@ -990,6 +990,7 @@
 valid_filenames.extend( map( lambda f: '%s.rst' % f, readme_files ) )
 valid_filenames.extend( readme_files )
 valid_filenames.append( '%s.txt' % repository_name )
+valid_filenames.append( '%s.rst' % repository_name )
 return valid_filenames

 def get_relative_path_to_repository_file( root, name,
relative_install_dir, work_dir, shed_config_dict,
resetting_all_metadata_on_repository ):

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Re: [galaxy-dev] Bug report

2013-07-24 Thread James Taylor
What format are the files you are trying to process with MACS in?

--
James Taylor, Assistant Professor, Biology/CS, Emory University


On Tue, Jul 23, 2013 at 3:05 PM, Jianxin Wang jianxin.w...@oicr.on.ca wrote:
 Hi Galaxy dev team,

 I'm trying to the you galaxy server to do NGS peak calling: Tools - NGS:
 Peak Calling -
 MACS Model-based Analysis of ChIP-Seq. However, the page won't allow me
 enter the input files: the drop-down selection box won't show anything when
 clicked. Same problem for the ChIP-Seq Control File selection box. Please
 take a look and email me back if problem is fixed.


 Jianxin Wang, PhD
 Computational Biologist
 jianxin.w...@oicr.on.ca

 Ontario Institute for Cancer Research
 MaRS Centre, South Tower
 101 College Street, Suite 800
 Toronto, Ontario, Canada M5G 0A3

 Tel:   416-673-8569
 www.oicr.on.ca

 This message and any attachments may contain confidential and/or privileged
 information for the sole use of the intended recipient. Any review or
 distribution by anyone other than the person for whom it was originally
 intended is strictly prohibited. If you have received this message in error,
 please contact the sender and delete all copies. Opinions, conclusions or
 other information contained in this message may not be that of the
 organization.

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Re: [galaxy-dev] Cloudman - is it possible to still use depreciated AMIs ?

2013-07-24 Thread Ravpreet Setia
The cluster status log has the following:



  *   14:49:00 - Setting up SGE...
  *   14:49:09 - Postgres service prerequisites OK; starting the service
  *   14:49:10 - PostgreSQL daemon NOT running.
  *   14:49:10 - PostgreSQL daemon NOT running.
  *   14:50:11 - --- PROBLEM, running command '/bin/su - postgres -c 
/usr/lib/postgresql/8.4/bin/pg_ctl -w -D /mnt/galaxy/db -l /tmp/pgSQL.log 
-o\-p 5910\ start' returned code '1' and following stderr: ''
  *   14:50:18 - PostgreSQL daemon NOT running.
  *   14:50:18 - Hadoop service prerequisites OK; starting the service
  *   14:50:31 - PostgreSQL daemon NOT running.
  *   14:50:45 - PostgreSQL daemon NOT running.
  *   14:50:58 - Done adding Hadoop service; service running.
  *   14:50:58 - PostgreSQL daemon NOT running.


From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] 
on behalf of Ravpreet Setia [ravpreet.se...@oicr.on.ca]
Sent: Tuesday, July 23, 2013 7:00 PM
To: Dannon Baker
Cc: galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] Cloudman - is it possible to still use depreciated 
AMIs ?

Hey, thanks for the response.

The ssh issue was on my end and is now resolved.

However, when I try the old AMI (ami-da58aab3), it mounts the new directory 
structure (meaning galaxyTools does not exist). Why does the old AMI have the 
directory structure of the new AMI?

Along with that, PostgreSQL does not load properly, it is stuck at Starting 
up. I get the following error:


FATAL:  database files are incompatible with server
DETAIL:  The data directory was initialized by PostgreSQL version 9.1, which is 
not compatible with this version 8.4.7.




From: Dannon Baker [dannon.ba...@gmail.com]
Sent: Monday, July 22, 2013 5:01 PM
To: Ravpreet Setia
Cc: galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] Cloudman - is it possible to still use depreciated 
AMIs ?

Yes, you can still use the deprecated AMIs.  What error are you seeing when you 
try to ssh in?


On Mon, Jul 22, 2013 at 4:30 PM, Ravpreet Setia 
ravpreet.se...@oicr.on.camailto:ravpreet.se...@oicr.on.ca wrote:
I tried launching an instance specifying ami-da58aab3 as the AMI but I am 
unable to SSH or access Cloudman using the instance's URL.

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[galaxy-dev] job_conf.xml workers

2013-07-24 Thread Ido Tamir
Hi,

what is the relationship between workers defined in universe and job_conf.xml? 
e.g.:

[server:handler0]
use = egg:Paste#http
port = 9092
host = 127.0.0.1
use_threadpool = true
threadpool_workers = 5


plugins workers=4
!-- workers is the number of threads for the runner's work queue.
 The default from plugins is used if not defined for a plugin.
  --
plugin id=local type=runner 
load=galaxy.jobs.runners.local:LocalJobRunner/
plugin id=drmaa type=runner 
load=galaxy.jobs.runners.drmaa:DRMAAJobRunner workers=10/
/plugins

a) do I have to specify the sum of the workers in job_conf.xml in universe?
e.g. 4 local, 10 drmaa = 14 threadpool_workers per handler? Or is universe 
overridden by job_conf.xml?

b)
How many concurrent jobs can I then expect to have concurrently? 14 per handler?


thank you very much,
ido




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Re: [galaxy-dev] BWA Illumina Mapping / BWA Reference Genome

2013-07-24 Thread Hans-Rudolf Hotz

Hi Moritz


I am not a galaxy tool shed expert, but I recommend you start with 
reading these wiki pages:


http://wiki.galaxyproject.org/Tool%20Shed

http://wiki.galaxyproject.org/InstallingRepositoriesToGalaxy


and then try installing BWA via the tool shed and ask (if any) specific 
questions (preferably with a new subject line) on the mailing list.



Regards  Hans-Rudolf


On 07/22/2013 11:20 AM, Moritz Juchler wrote:

Hey,

with the tool file line I got it working :)
Thanks a lot. Could you answer the question regarding Tool Shed: If I
would use Tool Shed, could I skip all these manual steps? Is the
installation of BWA with Tool Shed also this complicated or is it more
simle?

Best
Moritz


On 17 July 2013 09:51, Moritz Juchler juch...@stud.uni-heidelberg.de
mailto:juch...@stud.uni-heidelberg.de wrote:

Hey,

no thats correct I did not use Tool Shed. If I would use it, could I
skip all these manual steps? Is the installation of BWA with Tool
Shed also this complicated or is it more simle?

How did you not follow my steps :) I wrote down everything clearly,
at least thats what I was hoping.

And no I didnt write this line  tool
file=sr_mapping/bwa_wrapper.__xml / Thats the first time, I am
seeing this tutorial :( I will try this out right now

Best
Moritz


On 17 July 2013 09:42, Hans-Rudolf Hotz h...@fmi.ch
mailto:h...@fmi.ch wrote:

Hi Moritz

I am struggling to follow what exactly you have done. As far as
I can see, you did not use the toolshed
(http://wiki.galaxyproject.__org/Tool%20Shed
http://wiki.galaxyproject.org/Tool%20Shed) to install the BWA
alinger tool, but did all manually?

If so, have you added the following line:

  tool file=sr_mapping/bwa_wrapper.__xml /

to the tool_conf.xml file, and restarted Galaxy?


see also:
http://wiki.galaxyproject.org/__Admin/Tools/Add%20Tool%__20Tutorial
http://wiki.galaxyproject.org/Admin/Tools/Add%20Tool%20Tutorial



Hope this helps
Hans-Rudolf





On 07/16/2013 08:35 PM, Moritz Juchler wrote:

Hello Ladies and Gentlemen,

I am Moritz Juchler from University Heidelberg. For my
Bachelor thesis I
have to choose a bioinformatic pipeline management tool to
find SNP's in
genomes from hcc patients. My decision was made in favor of
galaxy. I
have a 64-bit openSuse 11.3 server.
I have installed Galaxy locally, since we have a) very large
files
(30GB per patient) and b) the data is protection sensitive.
I kept
close to http://wiki.galaxyproject.org/__Admin/Get%20Galaxy
http://wiki.galaxyproject.org/Admin/Get%20Galaxy
Now I would like to run this bpipe pipeline:
http://pastebin.com/sZd5vfdL
And the first step is to align my genome to a _hg19
reference genome_

which I have locally under /genedata/human_genome_GRCh37/__.

 trr@portalmoritz:~ ls -l /genedata/human_genome_GRCh37/
 total 8486312
 -rw-r--r-- 1 trr root 3199905909 2013-06-25 16:44 hg19.fa
 -rw-r--r-- 1 trr root   8591 2013-07-01 16:06
hg19.fa.amb
 -rw-r--r-- 1 trr root   4040 2013-07-01 16:06
hg19.fa.ann
 -rw-r--r-- 1 trr root 3137161344 2013-07-01 16:05
hg19.fa.bwt
 -rw-r--r-- 1 trr root  784290318 2013-07-01 16:06
hg19.fa.pac
 -rw-r--r-- 1 trr root 1568580688 2013-07-01 16:31
hg19.fa.sa http://hg19.fa.sa
 http://hg19.fa.sa


_bwa is installed and gives me:_


 trr@portalmoritz:~ bwa
 Program: bwa (alignment via Burrows-Wheeler transformation)
 Version: 0.7.5a-r405
 Contact: Heng Li l...@sanger.ac.uk
mailto:l...@sanger.ac.uk mailto:l...@sanger.ac.uk
mailto:l...@sanger.ac.uk



Then I tried to follow this guide:
http://wiki.galaxyproject.org/__Admin/NGS%20Local%20Setup
http://wiki.galaxyproject.org/Admin/NGS%20Local%20Setup to
get the
reference files and
http://wiki.galaxyproject.org/__Admin/Config/Tool%__20Dependencies
http://wiki.galaxyproject.org/Admin/Config/Tool%20Dependencies.

This is my _$PATH_


 trr@portalmoritz:~ echo $PATH


/home/trr/bin:/usr/local/bin:/__usr/bin:/bin:/usr/bin/X11:/__usr/X11R6/bin:/usr/games:/__home/trr/bpipe-0.9.8/bin:/__home/trr/bwa-0.7.5a:/home/trr/__samtools-0.1.19


_In the universe_wsgi.ini I changed:_


 tool_dependency_dir =
/home/trr/galaxy-dist/tool___dependency_dir
 debug = 

Re: [galaxy-dev] job_conf.xml workers

2013-07-24 Thread James Taylor
use_threadpool and threadpool_workers specify the number of threads
for handling web requests and have nothing to do with job running.

--
James Taylor, Assistant Professor, Biology/CS, Emory University


On Wed, Jul 24, 2013 at 11:02 AM, Ido Tamir ta...@imp.ac.at wrote:
 Hi,

 what is the relationship between workers defined in universe and 
 job_conf.xml? e.g.:

 [server:handler0]
 use = egg:Paste#http
 port = 9092
 host = 127.0.0.1
 use_threadpool = true
 threadpool_workers = 5


 plugins workers=4
 !-- workers is the number of threads for the runner's work queue.
  The default from plugins is used if not defined for a plugin.
   --
 plugin id=local type=runner 
 load=galaxy.jobs.runners.local:LocalJobRunner/
 plugin id=drmaa type=runner 
 load=galaxy.jobs.runners.drmaa:DRMAAJobRunner workers=10/
 /plugins

 a) do I have to specify the sum of the workers in job_conf.xml in universe?
 e.g. 4 local, 10 drmaa = 14 threadpool_workers per handler? Or is universe 
 overridden by job_conf.xml?

 b)
 How many concurrent jobs can I then expect to have concurrently? 14 per 
 handler?


 thank you very much,
 ido




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Re: [galaxy-dev] job_conf.xml workers

2013-07-24 Thread Ido Tamir
Ah yes,
this was local_job_queue_workers and cluster_job_queue_workers in universe.ini

thank you very much,
ido

On Jul 24, 2013, at 5:10 PM, James Taylor ja...@jamestaylor.org wrote:

 use_threadpool and threadpool_workers specify the number of threads
 for handling web requests and have nothing to do with job running.
 
 --
 James Taylor, Assistant Professor, Biology/CS, Emory University
 
 
 On Wed, Jul 24, 2013 at 11:02 AM, Ido Tamir ta...@imp.ac.at wrote:
 Hi,
 
 what is the relationship between workers defined in universe and 
 job_conf.xml? e.g.:
 
 [server:handler0]
 use = egg:Paste#http
 port = 9092
 host = 127.0.0.1
 use_threadpool = true
 threadpool_workers = 5
 
 
 plugins workers=4
!-- workers is the number of threads for the runner's work queue.
 The default from plugins is used if not defined for a plugin.
  --
plugin id=local type=runner 
 load=galaxy.jobs.runners.local:LocalJobRunner/
plugin id=drmaa type=runner 
 load=galaxy.jobs.runners.drmaa:DRMAAJobRunner workers=10/
 /plugins
 
 a) do I have to specify the sum of the workers in job_conf.xml in universe?
 e.g. 4 local, 10 drmaa = 14 threadpool_workers per handler? Or is universe 
 overridden by job_conf.xml?
 
 b)
 How many concurrent jobs can I then expect to have concurrently? 14 per 
 handler?
 
 
 thank you very much,
 ido
 
 
 
 
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Re: [galaxy-dev] HTML output with external Javascript

2013-07-24 Thread Stewart, Andrew
Hi Gromobir,

The file that Gift is producing is already an html file and there's
already an html datatype in Galaxy by default.  The output file in
question just invokes remote javascript libraries inside script tags,
and this seems to be tripping up Galaxy's data viewer.  We've tried
turning off the the html sanitizer, as mentioned below, but this doesn't
seem to have any effect.  We've tried downloading and referencing the
javascript libraries in /static/js as well.

I know that the new visualization platform in Galaxy uses a lot of
external javascript libraries like D3Š does anyone know how that is being
handled differently?


Thanks,
Andrew


--
Andrew Stewart
Office of Research Information Services (ORIS),
Office of the Chief Information Officer (OCIO),
Smithsonian Institution
202-633-2840




On 7/23/13 12:00 PM, galaxy-dev-requ...@lists.bx.psu.edu
galaxy-dev-requ...@lists.bx.psu.edu wrote:

On 07/23/2013 05:14 PM, Sinthong, Phanwadee wrote:
Hi galaxy-dev,

I have a tool that generates a HTML output file which uses some
javascript library. I cannot view the output file with the eye button in
Galaxy, but it still works fine from the HTML file opened out of the
file directory.
I changed the sanitize_all_html = False and restarted the galaxy server
as some earlier post suggested but still couldn't view the output file.
What else can I do to execute Javascript in the Galaxy browser?

Which library are you trying to use?
You may have to define your own datatype and add something like the
following to the file datatypes_conf.xmlafterwards:

datatype extension=html type=galaxay.datatypes.xml:Html
mimetype=application/html


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Re: [galaxy-dev] All processes are freezed

2013-07-24 Thread Dannon Baker
The best contact address for these kinds of issues is galaxy-b...@bx.psu.edu
.

There was a brief delay with some jobs running, but everything should be
working fine now.

-Dannon


On Wed, Jul 24, 2013 at 5:30 AM, Ali Sharifi Zarchi ashari...@gmail.comwrote:

 Hi everybody

 Since yesterday all processes I have run on Galaxy main are waiting. The
 system displayed a message to contact a Galaxy admin, but I could find no
 other way rather than this mailing list.

 Is this freeze due to a public maintenance, or it's just my problem?

 Best wishes,
 Ali

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Re: [galaxy-dev] uninstalling migrated tool

2013-07-24 Thread Dave Bouvier

Shantanu,

I've yet been unable to reproduce this issue on a local installation of 
a tool shed. If I do manage to reproduce it, I will re-open the issue 
and find a fix.


   --Dave B.

On 7/17/13 16:31:56.000, Greg Von Kuster wrote:

Hello Shantanu,

I've created a Trello card for this - we'll take a look as soon as possible.

https://trello.com/c/1hXOJPlZ/994-toolshed-from-shantanu-pavgi-on-7-12-13-it-seems-like-if-we-uninstall-a-migrated-tool-then-it-s-entry-in-the-migrated-tools-conf

Thanks,

Greg Von Kuster

On Jul 12, 2013, at 12:42 PM, Shantanu Pavgi (Campus) pa...@uab.edu wrote:



It seems like if we uninstall a migrated tool then it's entry in the 
migrated_tools_conf.xml file doesn't get deleted. Does it need to be removed 
manually?

--
Thanks,
Shantanu
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Re: [galaxy-dev] Bowtie2 error: Creates a 62 kb file.

2013-07-24 Thread Maddhi, Srinivas
[Copying the list].


-Original Message-
From: Maddhi, Srinivas Maddhi srinivas-mad...@uiowa.edu
Date: Wednesday, July 24, 2013 10:01 AM
To: Perez, Ricardo ricky_...@neo.tamu.edu
Subject: Re: [galaxy-dev] Bowtie2 error: Creates a 62 kb file.

Hi Ricardo,

Ensuring that the appropriate reference indices are present, properly
configured and selected when running bowtie2 might help address this
problem.

-Original Message-
From: Perez, Ricardo ricky_...@neo.tamu.edu
Date: Wednesday, July 24, 2013 1:35 AM
To: galaxy-dev@lists.bx.psu.edu galaxy-dev@lists.bx.psu.edu
Subject: [galaxy-dev] Bowtie2 error: Creates a 62 kb file.

Dear all,

Today we ran some problems when running bowtie2.  When we run bowtie2,
the run manages to complete.  However, bowtie2 creates a 62kb file.
The error log that we get is the following:

galaxy.jobs DEBUG 2013-07-23 17:09:42,378 (1483) Persisting job
destination (destination id: local:///)
galaxy.jobs.handler INFO 2013-07-23 17:09:42,392 (1483) Job dispatched
galaxy.tools DEBUG 2013-07-23 17:09:42,556 Building dependency shell
command for dependency 'bowtie2'
galaxy.tools WARNING 2013-07-23 17:09:42,556 Failed to resolve dependency
on 'bowtie2', ignoring
galaxy.tools DEBUG 2013-07-23 17:09:42,556 Building dependency shell
command for dependency 'samtools'
galaxy.tools WARNING 2013-07-23 17:09:42,556 Failed to resolve dependency
on 'samtools', ignoring
galaxy.jobs.runners.local DEBUG 2013-07-23 17:09:42,626 (1483) executing:
bowtie2 --version 
/usr/local/galaxy/galaxy-dist/database/tmp/GALAXY_VERSION_STRING_1483;
python /usr/local/galaxy/galaxy-dist/tools/sr_mapping/bowtie2_wrapper.py
--num-threads=4
--output=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1673.da
t
 
--indexes-path=/data/db/fungidb/Ncrassa_OR74A/bowtie2/Ncrassa_wt_Chr01.C
h
r07.+ChrM.+rDNA_v12  --single-paired=paired
--input1=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1668.da
t
 
--input2=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1669.da
t
 -I 0 -X 250  --settings=preSet
galaxy.jobs DEBUG 2013-07-23 17:09:42,696 (1483) Persisting job
destination (destination id: local:///)
galaxy.jobs.runners.local DEBUG 2013-07-23 17:09:42,766 execution
finished: bowtie2 --version 
/usr/local/galaxy/galaxy-dist/database/tmp/GALAXY_VERSION_STRING_1483;
python /usr/local/galaxy/galaxy-dist/tools/sr_mapping/bowtie2_wrapper.py
--num-threads=4
--output=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1673.da
t
 
--indexes-path=/data/db/fungidb/Ncrassa_OR74A/bowtie2/Ncrassa_wt_Chr01.C
h
r07.+ChrM.+rDNA_v12  --single-paired=paired
--input1=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1668.da
t
 
--input2=/usr/local/galaxy/galaxy-dist/database/files/001/dataset_1669.da
t
 -I 0 -X 250  --settings=preSet
galaxy.jobs.runners DEBUG 2013-07-23 17:09:42,850 executing external
set_meta script for job 1483:
/usr/local/galaxy/galaxy-dist/set_metadata.sh ./database/files
/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483 .
/usr/local/galaxy/galaxy-dist/universe_wsgi.ini
/usr/local/galaxy/galaxy-dist/database/tmp/tmpUiqpPD
/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483/gal
a
xy.json 
/usr/local/galaxy/galaxy-dist/database/job_working_directory/001/1483/met
a
data_in_HistoryDatasetAssociation_1767_wqhEmr,/usr/local/galaxy/galaxy-di
s
t/database/job_working_directory/001/1483/metadata_kwds_HistoryDatasetAss
o
ciation_1767_qVZjpG,/usr/local/galaxy/galaxy-dist/database/job_working_di
r
ectory/001/1483/metadata_out_HistoryDatasetAssociation_1767_vIfpRO,/usr/l
o
cal/galaxy/galaxy-dist/database/job_working_directory/001/1483/metadata_r
e
sults_HistoryDatasetAssociation_1767_UNl6OR,,/usr/local/galaxy/galaxy-dis
t
/database/job_working_directory/001/1483/metadata_over!
 ride_HistoryDatasetAssociation_1767_MZilrL
165.91.11.135 - - [23/Jul/2013:17:09:43 -0500] GET
/api/histories/ca8efa3c47500414 HTTP/1.1 200 -
http://galaxy.tamu.edu/history; Mozilla/5.0 (X11; Linux x86_64)
AppleWebKit/537.22 (KHTML, like Gecko) Ubuntu Chromium/25.0.1364.160
Chrome/25.0.1364.160 Safari/537.22
165.91.11.231 - - [23/Jul/2013:17:09:43 -0500] GET /history HTTP/1.1
200 - http://galaxy.tamu.edu/tool_runner/index; Mozilla/5.0 (Macintosh;
Intel Mac OS X 10_6_8) AppleWebKit/537.36 (KHTML, like Gecko)
Chrome/28.0.1500.71 Safari/537.36
165.91.11.231 - - [23/Jul/2013:17:09:43 -0500] GET
/api/histories/1ab12f1e464820dd HTTP/1.1 200 -
http://galaxy.tamu.edu/history; Mozilla/5.0 (Macintosh; Intel Mac OS X
10_6_8) AppleWebKit/537.36 (KHTML, like Gecko) Chrome/28.0.1500.71
Safari/537.36
165.91.11.231 - - [23/Jul/2013:17:09:43 -0500] GET
/history/get_display_application_links HTTP/1.1 200 -
http://galaxy.tamu.edu/history; Mozilla/5.0 (Macintosh; Intel Mac OS X
10_6_8) AppleWebKit/537.36 (KHTML, like Gecko) 

Re: [galaxy-dev] tool input is folder rather than file

2013-07-24 Thread Jeremy Goecks
You'll want to use composite datatypes:

http://wiki.galaxyproject.org/Admin/Datatypes/Composite%20Datatypes

On Jul 24, 2013, at 3:08 AM, Pankaj Narang wrote:

 Dear Developers,
 
 I want to design a tool whose input is folder containing files and 
 sub-folders.
 I was wandering which value should I assign to TYPE attribute of PARAM tag 
 in INPUTS.
 -- 
 Best regards,
 Pankaj Narang
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Re: [galaxy-dev] CloudMan Error

2013-07-24 Thread Deniz Erezyilmaz
Hi- there is a new problem: started 07/24/2013 21:16:27
  ./test_dependencies.sh
All required executables found in PATH
All required R packages are installed
Required module not found:
/mnt/galaxyData/custom/bin/python_libs/lib/python/pysam-0.6-py2.7-linux-x86_64.egg/csamtools.so:
undefined symbol: PyCapsule_New
Error in ./test_dependencies.sh: 256 at msg/Utils.pm line 25.




On Mon, Jul 22, 2013 at 5:04 PM, Dannon Baker dannon.ba...@gmail.comwrote:

 The warning message printed by the first ssh attempt indicates that you
 have no cloudman_key_pair.pem in the directory you executed the ssh command
 from.  Find this file (or create a new one) and you'll be able to ssh.

 Good luck, and please keep threads on the list instead of emailing
 directly.  Thanks!

 -Dannon

 On Mon, Jul 22, 2013 at 1:36 PM, deniz...@gmail.com wrote:

 Hi- the share string appears to be loading, but I am unable to ssh into
 my instance using the provided command, or anything like it. Instead I get
 the following:

 denizerezyilmaz$ ssh -i cloudman_key_pair.pem
 ubu...@ec2-54-242-61-164.compute-1.amazonaws.com
 Warning: Identity file cloudman_key_pair.pem not accessible: No such file
 or directory.

 OR:

  denizerezyilmaz$ ssh ec2-54-242-61-164.compute-1.amazonaws.com
 Permission denied (publickey).

 What am I missing? Thank you, Deniz


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[galaxy-dev] Lumi wrapper for Galaxy

2013-07-24 Thread Sachit Adhikari
Hello group,


I am trying to write lumi wrapper for Galaxy. Is anyone is writing or
already have written lumi wrapper for Galaxy. I couldn't find in both
galaxy main tool shed and test tool shed.

Regards

Sachit
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