Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Björn Grüning
Hi Vipin and Rui,

please have at the toolshed and test toolshed before developing
wrappers. For deseq2 there are already wrappers that are trying to solve
your issues and it would be nice if you could join that effort. Help
testing and contributing to it.

Ross has a cool wrapper of edgeR, DESeq2 and voom in one tool!

http://testtoolshed.g2.bx.psu.edu/view/fubar/differential_count_models

There is also that a DESeq2 single wrapper, more simplistic but
functional and with full installation of DESeq2, R3 in a reproducible
way.

http://testtoolshed.g2.bx.psu.edu/view/bgruening/deseq2

Please help Ross and me to make these wrapper rock solid!
Bjoern


 Hi All,
 
 
 we are making a wrapper for deseq2, and there is a step where
 control/experimental conditions
 need to be determined. For example, it would be a column like the
 following in the input file
 
 
 Type
 CTC
 CTC
 LM
 LM
 PT
 PT
 
 
 and we want the select list to contain
 
 
 CTC
 LM
 PT
 
 
 In other words, we want to filter out the 'Type' and have only
 distinct ones in the select list. We could
 make it in two steps, generate a intermediate file for this but it
 would be nice that we could directly
 retrieve the distinct types to make the list. I took a look at the
 tool config wiki, but didn't see anything
 (maybe because it was a quick scan). Does anyone have any tip on this?
 we'll really appreciate.
 
 
 Thanks,
 Rui
  
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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Joachim Jacob | VIB |

Hi Rui,

I should provide a 'sample metadata table', a tabular file with one 
column the names of your samples, the other column the associated 
metadata (e.g. type).


This is how I've done it in my DESeq2 wrapper. :-) Feel free to check 
the interface at http://toolshed.bits.vib.be/view/joachim/deseq2



Cheers,
Joachim

Joachim Jacob
Contact details: http://www.bits.vib.be/index.php/about/80-team


On 09/11/2013 01:15 AM, ruiwang.sz wrote:

Hi All,

we are making a wrapper for deseq2, and there is a step where 
control/experimental conditions
need to be determined. For example, it would be a column like the 
following in the input file


Type
CTC
CTC
LM
LM
PT
PT

and we want the select list to contain

CTC
LM
PT

In other words, we want to filter out the 'Type' and have only 
distinct ones in the select list. We could
make it in two steps, generate a intermediate file for this but it 
would be nice that we could directly
retrieve the distinct types to make the list. I took a look at the 
tool config wiki, but didn't see anything
(maybe because it was a quick scan). Does anyone have any tip on this? 
we'll really appreciate.


Thanks,
Rui


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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Peter Cock
Wow - that makes at least five Deseq2 wrappers for Galaxy
available or in progress :(

Bjoern's wrapper on the (test) tool shed,
http://testtoolshed.g2.bx.psu.edu/view/bgruening/deseq2

Ross' combined wrapper for edgeR, DESeq2 and voom in one tool:
http://testtoolshed.g2.bx.psu.edu/view/fubar/differential_count_models

Joachim's wrapper on the Tool Shed
http://toolshed.bits.vib.be/view/joachim/deseq2

Vipin's which is soon to be released to the (Test?) Tool Shed,
https://github.com/ratschlab/oqtans_tools/tree/master/DESeq2

And Rui's is working on one too.

This does seem like duplicated effort  a source of confusion
for end users and Galaxy administrators (a problem not unique
to deseq2, but affecting many Galaxy wrappers).

I appreciate there will be different needs, and one wrapper
may not suit all, but I would prefer if the default behaviour
for Galaxy tool wrapper authors was to collaborate on one
good wrapper rather than writing competing ones.

When I started work on a new wrapper I tried to announce
this on the mailing list to find out if anyone else was already
tackling the same tool - and that seemed to work quite well.
Of course, the volume of emails on galaxy-dev has grown
quite a lot over the last few years so that may not be as
effective, but the archives should be searchable.

Perhaps we need to improve communication in some way?
One option would be to encourage greater use of the Test
Tool Shed for works in progress to give them visibility?
You could even have the Tool Shed itself require a search
step before creating a new repository to avoid accidental
duplication of effort? Or maybe a wiki page of wrappers
in progress?

(And maybe we should split this into a new thread)

Regards,

Peter


On Wed, Sep 11, 2013 at 8:59 AM, Joachim Jacob | VIB |
joachim.ja...@vib.be wrote:
 Hi Rui,

 I should provide a 'sample metadata table', a tabular file with one column
 the names of your samples, the other column the associated metadata (e.g.
 type).

 This is how I've done it in my DESeq2 wrapper. :-) Feel free to check the
 interface at http://toolshed.bits.vib.be/view/joachim/deseq2


 Cheers,
 Joachim

 Joachim Jacob
 Contact details: http://www.bits.vib.be/index.php/about/80-team



 On 09/11/2013 01:15 AM, ruiwang.sz wrote:

 Hi All,

 we are making a wrapper for deseq2, and there is a step where
 control/experimental conditions
 need to be determined. For example, it would be a column like the
 following in the input file

 Type
 CTC
 CTC
 LM
 LM
 PT
 PT

 and we want the select list to contain

 CTC
 LM
 PT

 In other words, we want to filter out the 'Type' and have only distinct
 ones in the select list. We could
 make it in two steps, generate a intermediate file for this but it would
 be nice that we could directly
 retrieve the distinct types to make the list. I took a look at the tool
 config wiki, but didn't see anything
 (maybe because it was a quick scan). Does anyone have any tip on this?
 we'll really appreciate.

 Thanks,
 Rui


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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Joachim Jacob | VIB |
It is not necessarily bad. If we all test each others tools 
(dependencies, interface, output,...) we might get to the best of all 
worlds :-)  (I have planned to test Björns wrapper)


One of the points of differentiation is also how 'integrated' a tool 
needs to be: e.g. Ross' tool for diff expression (combining a complete 
pipelin), and my tool just on DE with DESeq2. I like to keep things 
granular on the level of the tools.



Cheers,
Joachim

Joachim Jacob.
Contact details: http://www.bits.vib.be/index.php/about/80-team


On 09/11/2013 10:56 AM, Peter Cock wrote:

Wow - that makes at least five Deseq2 wrappers for Galaxy
available or in progress :(

Bjoern's wrapper on the (test) tool shed,
http://testtoolshed.g2.bx.psu.edu/view/bgruening/deseq2

Ross' combined wrapper for edgeR, DESeq2 and voom in one tool:
http://testtoolshed.g2.bx.psu.edu/view/fubar/differential_count_models

Joachim's wrapper on the Tool Shed
http://toolshed.bits.vib.be/view/joachim/deseq2

Vipin's which is soon to be released to the (Test?) Tool Shed,
https://github.com/ratschlab/oqtans_tools/tree/master/DESeq2

And Rui's is working on one too.

This does seem like duplicated effort  a source of confusion
for end users and Galaxy administrators (a problem not unique
to deseq2, but affecting many Galaxy wrappers).

I appreciate there will be different needs, and one wrapper
may not suit all, but I would prefer if the default behaviour
for Galaxy tool wrapper authors was to collaborate on one
good wrapper rather than writing competing ones.

When I started work on a new wrapper I tried to announce
this on the mailing list to find out if anyone else was already
tackling the same tool - and that seemed to work quite well.
Of course, the volume of emails on galaxy-dev has grown
quite a lot over the last few years so that may not be as
effective, but the archives should be searchable.

Perhaps we need to improve communication in some way?
One option would be to encourage greater use of the Test
Tool Shed for works in progress to give them visibility?
You could even have the Tool Shed itself require a search
step before creating a new repository to avoid accidental
duplication of effort? Or maybe a wiki page of wrappers
in progress?

(And maybe we should split this into a new thread)

Regards,

Peter


On Wed, Sep 11, 2013 at 8:59 AM, Joachim Jacob | VIB |
joachim.ja...@vib.be wrote:

Hi Rui,

I should provide a 'sample metadata table', a tabular file with one column
the names of your samples, the other column the associated metadata (e.g.
type).

This is how I've done it in my DESeq2 wrapper. :-) Feel free to check the
interface at http://toolshed.bits.vib.be/view/joachim/deseq2


Cheers,
Joachim

Joachim Jacob
Contact details: http://www.bits.vib.be/index.php/about/80-team



On 09/11/2013 01:15 AM, ruiwang.sz wrote:

Hi All,

we are making a wrapper for deseq2, and there is a step where
control/experimental conditions
need to be determined. For example, it would be a column like the
following in the input file

Type
CTC
CTC
LM
LM
PT
PT

and we want the select list to contain

CTC
LM
PT

In other words, we want to filter out the 'Type' and have only distinct
ones in the select list. We could
make it in two steps, generate a intermediate file for this but it would
be nice that we could directly
retrieve the distinct types to make the list. I took a look at the tool
config wiki, but didn't see anything
(maybe because it was a quick scan). Does anyone have any tip on this?
we'll really appreciate.

Thanks,
Rui


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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Peter Cock
On Wed, Sep 11, 2013 at 10:12 AM, Joachim Jacob | VIB |
joachim.ja...@vib.be wrote:
 It is not necessarily bad. If we all test each others tools (dependencies,
 interface, output,...) we might get to the best of all worlds :-)  (I have
 planned to test Björns wrapper)

 One of the points of differentiation is also how 'integrated' a tool needs
 to be: e.g. Ross' tool for diff expression (combining a complete pipelin),
 and my tool just on DE with DESeq2. I like to keep things granular on the
 level of the tools.

That is a good reason for different wrappers, one for the tool itself,
and one for a larger pipeline using the same tool. And both can
declare a binary package dependency via the Tool Shed using
the binary dependency package Bjoern is working on, which I
assume will be released on the Test  Main Tool Shed under
the shared IUC account once ready:

http://testtoolshed.g2.bx.psu.edu/view/bgruening/package_deseq2_1_0_17

Regards,

Peter

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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Bjoern Gruening
Am Mittwoch, den 11.09.2013, 10:21 +0100 schrieb Peter Cock:
 On Wed, Sep 11, 2013 at 10:12 AM, Joachim Jacob | VIB |
 joachim.ja...@vib.be wrote:
  It is not necessarily bad. If we all test each others tools (dependencies,
  interface, output,...) we might get to the best of all worlds :-)  (I have
  planned to test Björns wrapper)
 
  One of the points of differentiation is also how 'integrated' a tool needs
  to be: e.g. Ross' tool for diff expression (combining a complete pipelin),
  and my tool just on DE with DESeq2. I like to keep things granular on the
  level of the tools.
 
 That is a good reason for different wrappers, one for the tool itself,
 and one for a larger pipeline using the same tool. And both can
 declare a binary package dependency via the Tool Shed using
 the binary dependency package Bjoern is working on, which I
 assume will be released on the Test  Main Tool Shed under
 the shared IUC account once ready:
 
 http://testtoolshed.g2.bx.psu.edu/view/bgruening/package_deseq2_1_0_17


Yes that is right, at the moment I have patches for the toolshed in
preparation for a unified and easy installation of R dependencies, once
that is done it will be under the IUC account. 

I don't see the need for 5 different wrappers. Ross approach deserves an
own wrapper I think. I thought a lot about if we should use it, since it
can compare 3 different approaches for DE, but it comes with some
complexity. We spend some time in trying to get the UI correct and
investigated some possibilities. Currently we have a few guys testing it
and giving comments to our UI idea. If the ownershop is a problem, we
probably can move the wrappers to a different account. But please lets
merge the code base and start communicating about the UI. 

Thanks,
Bjoern 

 Regards,
 
 Peter
 
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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Vipin TS
Yes I am agreeing on all statements.
Let me finish my package and will add in one of the toolshed by today
evening. I also willing to test other wrappers to improve the additional
parameter settings.

Tool wrappers Work In Progress from my side:
DEXSeq
edgeR
Scripture
STAR aligner
SAFT multi mapper resolution

This will be adding soon to the toolshed.

Thanks to all, Vipin

On Wed, Sep 11, 2013 at 6:08 AM, Bjoern Gruening
bjoern.gruen...@gmail.comwrote:

 Am Mittwoch, den 11.09.2013, 10:21 +0100 schrieb Peter Cock:
  On Wed, Sep 11, 2013 at 10:12 AM, Joachim Jacob | VIB |
  joachim.ja...@vib.be wrote:
   It is not necessarily bad. If we all test each others tools
 (dependencies,
   interface, output,...) we might get to the best of all worlds :-)  (I
 have
   planned to test Björns wrapper)
  
   One of the points of differentiation is also how 'integrated' a tool
 needs
   to be: e.g. Ross' tool for diff expression (combining a complete
 pipelin),
   and my tool just on DE with DESeq2. I like to keep things granular on
 the
   level of the tools.
 
  That is a good reason for different wrappers, one for the tool itself,
  and one for a larger pipeline using the same tool. And both can
  declare a binary package dependency via the Tool Shed using
  the binary dependency package Bjoern is working on, which I
  assume will be released on the Test  Main Tool Shed under
  the shared IUC account once ready:
 
  http://testtoolshed.g2.bx.psu.edu/view/bgruening/package_deseq2_1_0_17


 Yes that is right, at the moment I have patches for the toolshed in
 preparation for a unified and easy installation of R dependencies, once
 that is done it will be under the IUC account.

 I don't see the need for 5 different wrappers. Ross approach deserves an
 own wrapper I think. I thought a lot about if we should use it, since it
 can compare 3 different approaches for DE, but it comes with some
 complexity. We spend some time in trying to get the UI correct and
 investigated some possibilities. Currently we have a few guys testing it
 and giving comments to our UI idea. If the ownershop is a problem, we
 probably can move the wrappers to a different account. But please lets
 merge the code base and start communicating about the UI.

 Thanks,
 Bjoern

  Regards,
 
  Peter
 
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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Ross
Vipin,
Search works pretty well on the toolshed - I'd recommend a search for (eg)
'star' before writing yet another rnastar toolshed wrapper and dependency
package? There's an installable fubar package_rnastar and an rnastar
wrapper based on Jeremy's code mentioned below - but working AFAIK - at
least it installs and works fine here and on the GVL.



On Thu, Sep 12, 2013 at 1:49 AM, Jeremy Goecks jeremy.goe...@emory.eduwrote:

 STAR aligner


 I have a partial STAR wrapper that may be useful:
 https://bitbucket.org/jgoecks/jeremys-code/src/d7096c132657420f08b540c6954b947f648aac80/galaxy/tool-wrappers/star.xml?at=default

 Whether you use this code or not, I do have one request: please put the
 output in BAM rather than SAM format in order to save disk space.

 Best,
 J.

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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Jeremy Goecks
 STAR aligner 

I have a partial STAR wrapper that may be useful: 
https://bitbucket.org/jgoecks/jeremys-code/src/d7096c132657420f08b540c6954b947f648aac80/galaxy/tool-wrappers/star.xml?at=default

Whether you use this code or not, I do have one request: please put the output 
in BAM rather than SAM format in order to save disk space.

Best,
J.___
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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread ruiwang.sz
Hi Joachim,

Thanks for the info! but I could not register on your instance...and thus
could not check your wrapper. :-( Please help!

Thanks,
Rui


On Wed, Sep 11, 2013 at 12:59 AM, Joachim Jacob | VIB | 
joachim.ja...@vib.be wrote:

 Hi Rui,

 I should provide a 'sample metadata table', a tabular file with one column
 the names of your samples, the other column the associated metadata (e.g.
 type).

 This is how I've done it in my DESeq2 wrapper. :-) Feel free to check the
 interface at 
 http://toolshed.bits.vib.be/**view/joachim/deseq2http://toolshed.bits.vib.be/view/joachim/deseq2


 Cheers,
 Joachim

 Joachim Jacob
 Contact details: 
 http://www.bits.vib.be/index.**php/about/80-teamhttp://www.bits.vib.be/index.php/about/80-team



 On 09/11/2013 01:15 AM, ruiwang.sz wrote:

 Hi All,

 we are making a wrapper for deseq2, and there is a step where
 control/experimental conditions
 need to be determined. For example, it would be a column like the
 following in the input file

 Type
 CTC
 CTC
 LM
 LM
 PT
 PT

 and we want the select list to contain

 CTC
 LM
 PT

 In other words, we want to filter out the 'Type' and have only distinct
 ones in the select list. We could
 make it in two steps, generate a intermediate file for this but it would
 be nice that we could directly
 retrieve the distinct types to make the list. I took a look at the tool
 config wiki, but didn't see anything
 (maybe because it was a quick scan). Does anyone have any tip on this?
 we'll really appreciate.

 Thanks,
 Rui


 __**_
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 and other Galaxy lists, please use the interface at:
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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread ruiwang.sz
Hey Guys,

It's great to receive so many responses! Thank you all for the
information...
and let me check out each of the masterpiece. :-)

Best,
Rui


On Wed, Sep 11, 2013 at 1:56 AM, Peter Cock p.j.a.c...@googlemail.comwrote:

 Wow - that makes at least five Deseq2 wrappers for Galaxy
 available or in progress :(

 Bjoern's wrapper on the (test) tool shed,
 http://testtoolshed.g2.bx.psu.edu/view/bgruening/deseq2

 Ross' combined wrapper for edgeR, DESeq2 and voom in one tool:
 http://testtoolshed.g2.bx.psu.edu/view/fubar/differential_count_models

 Joachim's wrapper on the Tool Shed
 http://toolshed.bits.vib.be/view/joachim/deseq2

 Vipin's which is soon to be released to the (Test?) Tool Shed,
 https://github.com/ratschlab/oqtans_tools/tree/master/DESeq2

 And Rui's is working on one too.

 This does seem like duplicated effort  a source of confusion
 for end users and Galaxy administrators (a problem not unique
 to deseq2, but affecting many Galaxy wrappers).

 I appreciate there will be different needs, and one wrapper
 may not suit all, but I would prefer if the default behaviour
 for Galaxy tool wrapper authors was to collaborate on one
 good wrapper rather than writing competing ones.

 When I started work on a new wrapper I tried to announce
 this on the mailing list to find out if anyone else was already
 tackling the same tool - and that seemed to work quite well.
 Of course, the volume of emails on galaxy-dev has grown
 quite a lot over the last few years so that may not be as
 effective, but the archives should be searchable.

 Perhaps we need to improve communication in some way?
 One option would be to encourage greater use of the Test
 Tool Shed for works in progress to give them visibility?
 You could even have the Tool Shed itself require a search
 step before creating a new repository to avoid accidental
 duplication of effort? Or maybe a wiki page of wrappers
 in progress?

 (And maybe we should split this into a new thread)

 Regards,

 Peter


 On Wed, Sep 11, 2013 at 8:59 AM, Joachim Jacob | VIB |
 joachim.ja...@vib.be wrote:
  Hi Rui,
 
  I should provide a 'sample metadata table', a tabular file with one
 column
  the names of your samples, the other column the associated metadata (e.g.
  type).
 
  This is how I've done it in my DESeq2 wrapper. :-) Feel free to check the
  interface at http://toolshed.bits.vib.be/view/joachim/deseq2
 
 
  Cheers,
  Joachim
 
  Joachim Jacob
  Contact details: http://www.bits.vib.be/index.php/about/80-team
 
 
 
  On 09/11/2013 01:15 AM, ruiwang.sz wrote:
 
  Hi All,
 
  we are making a wrapper for deseq2, and there is a step where
  control/experimental conditions
  need to be determined. For example, it would be a column like the
  following in the input file
 
  Type
  CTC
  CTC
  LM
  LM
  PT
  PT
 
  and we want the select list to contain
 
  CTC
  LM
  PT
 
  In other words, we want to filter out the 'Type' and have only distinct
  ones in the select list. We could
  make it in two steps, generate a intermediate file for this but it would
  be nice that we could directly
  retrieve the distinct types to make the list. I took a look at the tool
  config wiki, but didn't see anything
  (maybe because it was a quick scan). Does anyone have any tip on this?
  we'll really appreciate.
 
  Thanks,
  Rui
 
 
  ___
  Please keep all replies on the list by using reply all
  in your mail client.  To manage your subscriptions to this
  and other Galaxy lists, please use the interface at:
 http://lists.bx.psu.edu/
 
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Re: [galaxy-dev] Deseq2 wrapper question

2013-09-10 Thread Vipin TS
Hello Rui,

We have DESeq2 wrapper in our oqtans package and you will find this at
https://github.com/ratschlab/oqtans_tools/tree/master/DESeq2

I am currently creating the associated tool_dependency.xml file for dumping
the package in galaxy toolshed. otherwise it requires some manual effort to
install via toolshed.

May be it will help you,
Vipin


Hi All,

 we are making a wrapper for deseq2, and there is a step where
 control/experimental conditions
 need to be determined. For example, it would be a column like the
 following in the input file

 Type
 CTC
 CTC
 LM
 LM
 PT
 PT

 and we want the select list to contain

 CTC
 LM
 PT

 In other words, we want to filter out the 'Type' and have only distinct
 ones in the select list. We could
 make it in two steps, generate a intermediate file for this but it would
 be nice that we could directly
 retrieve the distinct types to make the list. I took a look at the tool
 config wiki, but didn't see anything
 (maybe because it was a quick scan). Does anyone have any tip on this?
 we'll really appreciate.

 Thanks,
 Rui


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