[galaxy-user] BWA question on Mac OS X 10.7.2

2011-11-11 Thread Christopher Callaway
Hi,

I have tried to install BWA on my MacPro.  Once installed, I typed the command 
make and it did not work.  When installing BWA to a Linux machine, it worked 
fine.  How do I get around this problem?

Thanks,

CWC
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[galaxy-user] Galaxy local install problems understanding where to put mouse ref seq data to populate ref genome in BWA

2011-11-09 Thread Christopher Callaway
Hello,

I am still having problems understanding how to install mm9 mouse ref genome 
into our own install of Galaxy.  When I try to map with BWA for Illumina the 
drop down for select ref genome is not populated with anything.  How do you 
grab the mm9 ref file and how do you convert it so that Galaxy local "sees" it? 
How do you build the index and how do you prepare the loc file? Our install 
does not contain a folder tool-data/shared/ucsc/builds.txt.

Sorry but I am not a programmer, just a bench scientist.

Thanks in advance,

Christopher W. Callaway
University of Utah
Dept. of Pediatrics
Division of Neonatology
417 Wakara Way
#
Salt Lake City, UT 84108

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[galaxy-user] BWA install problem

2011-11-04 Thread Christopher Callaway
Hi,

I am trying to run Galaxy locally and downloaded BWA but I can't get it to run. 
 Do I have to use 0.5.6 or can I use 0.5.9? The make command does not work with 
ver. 0.5.9.

Thanks,

Christopher W. Callaway
University of Utah
Dept. of Pediatrics
Division of Neonatology
417 Wakara Way
#
Salt Lake City, UT 84108

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[galaxy-user] customizing mm9 mouse reference genome and then using that as the ref genome when uploading Illumina data

2011-10-13 Thread Christopher Callaway
I would like to filter out the mm9 mouse build ref genome to only show MspI 
sites, and then use that as my "Genome" when uploading my Illumina data.  
Anyone have a protocol for that?

Thanks,

Christopher W. Callaway
University of Utah
Dept. of Pediatrics
Division of Neonatology
417 Wakara Way
#
Salt Lake City, UT 84108

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