Re: [Histonet] Re: Which one first - antigen retrieval or peroxidase block
As far as I remember enzymes are proteins and are they not supposed to cross-link with formalin also? René J. --- On Mon, 9/29/08, Greg Dobbin [EMAIL PROTECTED] wrote: From: Greg Dobbin [EMAIL PROTECTED] Subject: Re: [Histonet] Re: Which one first - antigen retrieval or peroxidase block To: [EMAIL PROTECTED], histonet@lists.utsouthwestern.edu Date: Monday, September 29, 2008, 10:12 PM Peroxidase is an enzyme. It doesn't miraculously appear as a result to exposure to heat. You show me one one slide where this has occurred and I will humbly remain silent in this forum for a whole month! Ok, maybe just a week... :-) Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 And in the end it's not the years in your life that count. It's the life in your years. - Abraham Lincoln [EMAIL PROTECTED] 09/29/08 5:59 PM I'm with Rene on this one. Block after retrieval. For those of you who do the block before retrieval, how do you control for any activity that might be exposed during the retrieval step? Couldn't it lead to false positives in your slides? Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **Looking for simple solutions to your real-life financial challenges? Check out WalletPop for the latest news and information, tips and calculators. (http://www.walletpop.com/?NCID=emlcntuswall0001) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Careers Bulletin 9-30-08
Hello Histonetters! It is hard to believe the summer is over already. Before we know it, it will be 2009! Lets Get The Ball Rolling!!! Looking for a new job in the Fall? Planning a job change after the holidays? Considering making a move in 2009? Are you a histo tech looking for a better opportunity? Are you a new or recent graduate of a histology school? Are you a traveler considering transitioning into a permanent position? Are you a Histo Tech looking for an opportunity to advance into management? Lets Get The Ball Rolling!!! Do we need to freshen up your resume? I can help!! Do you need help with what your next career move should be? I can help!! Do you need assistance with salary negotiation? I can help!! Do you want someone to work with you on what to say in an interview? I can help!! How about coordinating times and dates for the interview? I can help!! All of the positions that I work with are full time permanent positions with premier companies setting the mark to be employers of choice in their respective areas. My clients offer competitive salaries excellent benefits and relocation assistance. If you or anyone you know is considering making a job change all it takes is a 5 minute phone call or a quick e-mail to Get The Ball Rolling!!! We can talk about my current openings and what you are looking for in your next position. Here are some of my current openings: HISTOLOGY MANAGEMENT: Manchester, NH Brand new Pathology Lab Buffalo NY Histology Manager for Renown Clinical and Research Facility Portland, OR Histology and Cytology Manager no bench work. Los Angeles, CA Afternoon shift for National Reference Lab El Paso, TX Lab Supervisor for hospital environment HISTOTECHS Washington DC Histotech Prestigious Hospital Environment PA- Pittsburgh, Dayshift HT or HTL required. TX - Austin, Pathology Lab Entry Level and experience techs needed TX - Dallas , Histotechnician/Histotechnologist TX El Paso, Histo tech WA Seattle Clinical Positions on Night Shift $$$ Shift diff!! WA Seattle IHC Tech WA - Seattle area, Research position WA - Spokane, Clinical Position in Brand new lab! MD - Baltimore, Grossing Histotechnologist CA - Los Angeles, Histotechnician/Histotechnologist ASCP cert req MA North of Boston, IHC Tech NY New York City Flow Cytometry Tech NY Uniondale Histotech Specialty Lab Environment OTHER POSITIONS FL Tampa Pathologist Assistant NY New York City, Flow Cytometry Tech WA Seattle, IHC Tech MA North of Boston IHC Tech Remember it never hurts to look! If you or someone you know is interested in any of these opportunities please let me know. Also please feel free to forward this e-mail to your friends and colleagues as well I can be reached toll free at 866-60-RELIA (866-607-3542) or [EMAIL PROTECTED] Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:(407)353-5070 FAX:(407)678-2788 Toll Free: (866)607-3542 e-mail: mailto:[EMAIL PROTECTED] [EMAIL PROTECTED] http://home.earthlink.net/~relia1 http://home.earthlink.net/~relia1 http://www.myspace.com/pamatrelia www.myspace.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Great opportunity for Histotechnician in brand new laboratory!
Great opportunity for Histotechnician in brand new laboratory! Gastrointestinal Associates of North Texas in Ft Worth , is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and meet CLIA-88 regulations to perform gross dissection. Prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers competitive salary, medical insurance, retirement plan, and vacation /sick leave. Interested applicants should e-mail resumes to Meredith Hale at [EMAIL PROTECTED] . Meredith Hale HT (ASCP) Technical Director Caris Diagnostics 8400 Esters Blvd. Ste. 190 Irving, Texas 75063 469-648-8253 Cell 214-596-2219 Office 972-929-9996 Fax Angela Martinez Internal Recruiter Caris Diagnostics 8400 Esters Blvd. Suite 190 Irving, Texas 75063 (214) 277-8700 Phone (214) 596-7490Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Lot Logs
I never did that BUT if you have a problem during any step of your process it is a good practice to consult the supplier with the lot number of their products to find out if the problem you experienced has been reported by others also. René J. --- On Tue, 9/30/08, Matt Roark [EMAIL PROTECTED] wrote: From: Matt Roark [EMAIL PROTECTED] Subject: [Histonet] Lot Logs To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 30, 2008, 11:01 AM Does everyone keep logs of lot numbers for their xylene and alcohol bottles? I can see keeping track of lot numbers for stains and antibodies but I can not find in the CAP checklist where they would want us to keep track of the lots of xylene and or alcohol. What does everyone else do? Thanks!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PLS Help with IHC protocol
Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block Can someone break this down for me in simple instructions? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PLS Help with IHC protocol
Kim,
We need to know what the concentration of the antibody is before you make it
10ug/ml
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email [EMAIL PROTECTED]
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
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-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Kimberly
Tuttle
Sent: Tuesday, September 30, 2008 9:25 AM
To: [EMAIL PROTECTED]
Subject: [Histonet] PLS Help with IHC protocol
Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block
Can someone break this down for me in simple instructions?
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax
This e-mail and any accompanying attachments may be privileged,
confidential, contain protected health information about an identified
patient or be otherwise protected from disclosure. State and federal law
protect the confidentiality of this information. If the reader of this
message is not the intended recipient; you are prohibited from using,
disclosing, reproducing or distributing this information; you should
immediately notify the sender by telephone or e-mail and delete this e-mail.
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RE: [Histonet] Lot Logs
No, not sure my supplier even furnishes lot numbers on that.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email [EMAIL PROTECTED]
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa
Sent: Tuesday, September 30, 2008 9:13 AM
To: histonet@lists.utsouthwestern.edu; Matt Roark
Subject: Re: [Histonet] Lot Logs
I never did that BUT if you have a problem during any step of your process
it is a good practice to consult the supplier with the lot number of their
products to find out if the problem you experienced has been reported by
others also.
René J.
--- On Tue, 9/30/08, Matt Roark [EMAIL PROTECTED] wrote:
From: Matt Roark [EMAIL PROTECTED]
Subject: [Histonet] Lot Logs
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 30, 2008, 11:01 AM
Does everyone keep logs of lot numbers for their xylene and alcohol bottles?
I can see keeping track of lot numbers for stains and antibodies but I can
not find in the CAP checklist where they would want us to keep track of the
lots of xylene and or alcohol. What does everyone else do?
Thanks!!
Matthew Roark
Histology Specialist -B.S,HT(ASCP)CM
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
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[Histonet] Control blocks for Special Stains
We are looking at making our own SS blocks from fresh tissue injected with organisms we can get from our Micro dept. Any advice on this..what type of fresh tissue?, etc. Jeanne Jeanne Clark HT/MLT (ASCP) Pathology Manager Mission Hospitals 428 Biltmore Ave. Asheville, NC. 28801 828-213-1213 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Amyloid Controls
Hi Everyone~ We are having a hard time finding Amyloid controls. Can anyone recommend a company that has good controls? Thanks~ Beth Beth Delescavage, BS, HTL (ASCP) QIHC DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking a Pathology Assistant temp tech as well as a local histologist
Hi everyone-a couple of temp openings for your viewing enjoyment! Seeking a fully qualified Pathology Assistant (not a histotech who can gross but a PA) for a travel position. Please call for specifics. Also seeking a local temp tech for Western Ohio. We're trying to help a facility that really needs the support but can't quite stretch for a full travel situation. If you live in Western Ohio and have been curious about temping, this might be a simple way to get dip your toes in the pool. Confidential conversations welcome-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone [EMAIL PROTECTED] www.fullstaff.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Colored paraffin
Several years ago we purchased colored paraffin from Polysciences. We got both pink and amethyst (purple). They had other colors. The colors were great for small biopsies. However, if we used colored cassettes (green, yellow, lavender, blue) the paraffin darkened and we could hardly see the tissue. So it's a double edge sword. If we used white cassettes all the time, the colored paraffin would be great. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Career Opening!
We have an opening for a full time Histotechnican/Histotechnologist M-F 8:30am - 5:00pm or 9am-5:30pm, with occasional Saturdays. We have excellent pay, benefits, retirement plan, PTO, and many other perks. We are a non-profit hospital located in York, Pennsylvania. We do about 40,000 cases a year. We are 30 minutes from Harrisburg, one hour from Baltimore, about 2 hours from Philadelphia. Go to our website at www.wellspan.org http://www.wellspan.org/ and search our careers page to submit a application. We look forward to hearing from you! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open. -John Moe Maturity is accepting imperfections. CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. __ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Metanil Yellow and Methyl Green
Dear Histonetters! I have a question regarding a dye called Metanil Yellow, can anyone suggest a good vendor for this dye (I know Sigma and Polyscientific sell it) and what are your impressions? Second question, I have asked long time regarding Methyl Green. I narrowed it down to DAKO ready to use, Polyscientific and also Vector. I did the usual stain for 5 mins and then quick rinse with 95% ethanol, and then 10 dips in series of 2 95%ethanols, 2X 100% and then 2 mins in 2 changes of Xylene and still have inconsistencies. Can someone suggest a good protocol? Thank you very much. Igor Deyneko Infinity Pharmaceuticals In-Vivo Pharmacology Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology or IHC conferences/workshops
Dear Histonetters! I was wondering where someone knows of any useful conferences or workshops , which deal with histological techniques, or Immunohistochemical techniques or even microscopy techniques( I heard about a place in Cape Cod), coming up in 2009??? Any information would be appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals In-Vivo Pharmacology Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
