[Histonet] Re: B5 fixative
Well, this pathologist was dragged screaming away from B-5 - and I've brewed plenty of it out of the dry chemicals myself - but you really can't use it any more. I was surprised to find out that it's still available, but it still is. I haven't found any of the substitutes to be worthwhile. The major advantage of B-5 was that it's a rapid fixative (for small specimens). Lymphoid tissue and marrow really need to be fixed overnight in neutral buffered formalin, for optimal fixation. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC for Amyloid L
Laurie, The most common cause in patients with aberrant light chain deposition (i.e. Amyloid L) is multiple myeloma. Staining with kappa and lambda should reveal the Amyloid L. Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center/ LabFirst 800 Montclair Road Birmingham, AL 35213-1984 [EMAIL PROTECTED] 205-592-5387 (office) 205-592-5388 (lab) 205-592-5646 (fax) Message: 3 Date: Wed, 15 Oct 2008 11:56:21 -0700 From: Laurie Colbert [EMAIL PROTECTED] Subject: [Histonet] IHC for Amyloid L To: histonet@lists.utsouthwestern.edu Message-ID: [EMAIL PROTECTED] om Content-Type: text/plain; charset=us-ascii Does anyone know of a reference lab that does an IHC stain for Amyloid L (amyloid light chain)? I can find labs that do Amyloid A and Amyloid P, but not Amyloid L. Laurie Colbert Huntington Hospital Pasadena, CA -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HT grossing specimens
Compensation for techs who can and do gross is 12% higher than non-grossing techs. As far as processingWe added that to our grossing manual, but if you are held to CLIA standards it is irrelevant. In my opinion, most labs will be better served to follow the guidelines of well documented training, direct or indirect supervision, a comprehensive list of what specimens can be grossed in by personnel other than pathologists, and yearly evaluation of all employees who gross or process. There is a great deal of added responsibility here and I believe we need to do everything we can to protect the techs who do it. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Snyder, Wendy Sent: Friday, October 17, 2008 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT grossing specimens I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT grossing specimens
Is that $1 per specimen or $1 per hour? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 [EMAIL PROTECTED] www.LMHealth.org -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Behalf Of Jesus Ellin Sent: Friday, October 17, 2008 11:38 AM To: Jesus Ellin; Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens But did not finish answering the question, there is a augmentation of pay depending on years of experience and specimen type,, most of these specimens are GI or Prostate so we give a $1.00 augmentation,, but most of the time the PA do the gross and the Pathologist still like doing the large specimens or complex ones. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 (Fax: (928) 336-7319 *Email: [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jesus Ellin Sent: Friday, October 17, 2008 8:35 AM To: Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just for everyone's FYI if you are Processing specimen's under CAP regulation, but you are collecting from Medicare, The Medicare regulation is completely different and if audited by Medicare can be subject to huge fines for not meeting the requirement.. Help me out on this one Charles E. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 (Fax: (928) 336-7319 *Email: [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Weems, Joyce Sent: Friday, October 17, 2008 8:30 AM To: Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just part of the gross room position here.. But it is called Processing according to the latest CAP check list... Joyce -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Snyder, Wendy Sent: Friday, October 17, 2008 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT grossing specimens I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
RE: [Histonet] HT grossing specimens
$1.00 dollar per hour Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 (Fax: (928) 336-7319 *Email: [EMAIL PROTECTED] -Original Message- From: Tom McNemar [mailto:[EMAIL PROTECTED] Sent: Friday, October 17, 2008 10:13 AM To: Jesus Ellin; Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Is that $1 per specimen or $1 per hour? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 [EMAIL PROTECTED] www.LMHealth.org -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Behalf Of Jesus Ellin Sent: Friday, October 17, 2008 11:38 AM To: Jesus Ellin; Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens But did not finish answering the question, there is a augmentation of pay depending on years of experience and specimen type,, most of these specimens are GI or Prostate so we give a $1.00 augmentation,, but most of the time the PA do the gross and the Pathologist still like doing the large specimens or complex ones. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 (Fax: (928) 336-7319 *Email: [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jesus Ellin Sent: Friday, October 17, 2008 8:35 AM To: Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just for everyone's FYI if you are Processing specimen's under CAP regulation, but you are collecting from Medicare, The Medicare regulation is completely different and if audited by Medicare can be subject to huge fines for not meeting the requirement.. Help me out on this one Charles E. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 (Fax: (928) 336-7319 *Email: [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Weems, Joyce Sent: Friday, October 17, 2008 8:30 AM To: Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just part of the gross room position here.. But it is called Processing according to the latest CAP check list... Joyce -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Snyder, Wendy Sent: Friday, October 17, 2008 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT grossing specimens I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not
[Histonet] fixative for brains?
I'm trying to revise our laboratories SOPs. They currently are using 20% NBF to fix mammalian brains and alcohol-formalin to fix avian brains. In the past, I've used 10% NBF for all brains. Does anyone have any input on this? I'd like to keep things simple, if possible, and just use one fixative. Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo opening in San Antonio, TX
Happy Friday! I have a new position open and wanted to keep you all informed. A facility I work closely with in San Antonio, TX has just asked for my help in finding a Histo tech. Basic histology, IHC experience and ASCP preferred. Salary range $48,000 - $53,000. Potential for relocation aid and sign on bonuses! If you or anyone you know would be interested in this position or any others I have available, please contact me. I specialize in perm placement, temp-to-perm and travel for laboratory professionals. If you have any questions about my company, services or the histology market, please feel free to give me a call or send an email. Thank you, hope to hear from you soon and have a great weekend! Kyla Nemitz TravelMax Medical Professionals - Maxim Healthcare Tel: 888.800.1855 or 813.371.5175 Fax: 800.294.1248 Search our jobs or apply online at: www.TravelMaxAllied.com http://www.travelmaxallied.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Susie Hargrove is out of the office.
I will be out of the office starting 10/17/2008 and will not return until 10/27/2008. I will respond to your message when I return. If immediate assistance is needed please call 3198. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] B5 fixative
I agree. The Occ Health and SAfety representative is your best bet to consign the B5 to history. Piero Nelva Anatomical pathology Monash Medical Centre Australia - Original Message - From: Richard Cartun [EMAIL PROTECTED] To: histonet@lists.utsouthwestern.edu; Bryan Watson [EMAIL PROTECTED] Sent: Saturday, October 18, 2008 12:46 AM Subject: Re: [Histonet] B5 fixative If you are having a hard time with a pathologist regarding the use of B5, get your safety people involved. They will take care of it. We stopped using B5 a very long time ago. We use formalin for all our lymph nodes and bone marrows. No one should be using B5 and manufacturers should stop making it. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Bryan Watson [EMAIL PROTECTED] 10/17/08 9:21 AM How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.1/1731 - Release Date: 10/17/2008 7:01 PM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question on Von Kossa
James, Try this: once the sections are brought to water, 5% silver nitrate in either bright sunlight or a 60-100 watt incandescent light bulb for 30 min (check for browning of the control tissue adjust time as needed). Rinse well in distilled water. 5% Sodium Thiosulfate for 1 min. Rinse and counterstain with nuclear fast red or whatever you think would look cool :-). Dehydrate, clear and coverslip the slides. Of course, as with any silver stain use acid cleaned glassware and gloves unless you like watching your fingers turn black in the sunlight. Really well decalcified tissue usually has the Calcium washed out. Try a calcified breast or something that wasn't decalcified. This causes microtomists to curse a lot, but it makes great VonKossa controls. Have fun, Amos Message: 10 Date: Thu, 16 Oct 2008 15:45:11 -0500 From: Herrick, James L. [EMAIL PROTECTED] Subject: [Histonet] Question on Von Kossa To: histonet@lists.utsouthwestern.edu Message-ID: [EMAIL PROTECTED] Content-Type: text/plain; charset=iso-8859-1 Hello everyone, Does anyone have a good Von Kossa stain protocol that they would not mind sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA or MMA (sections are between 5 and 10 µm thick)? I would appreciate it greatly. Thank you much. Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
