RE: [Histonet] used hacker bright cryostat...
Tom, We have a few used Bright OTF/AS models available but they are 230v 50 htz so will not suit your region in you are in the USA, if so hopefully one of our distributors will see your posting. Please take into account that it is very, very favorable to purchase from the UK at present. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abri...@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tbritten Sent: 06 January 2009 18:42 To: histonet@lists.utsouthwestern.edu Cc: csny...@scimedx.com Subject: [Histonet] used hacker bright cryostat... hello all; we are looking to purchase the above. if you have one around the lab not being used maybe this is a way to make some $ for another piece of equipment? please contact my colleague (charlie snyder 973 625-8822 x43) or above email if you wish. thanks and best regards, tom britten ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome Cutting Safety
I use my fingers too, so no help there. But an automated microtome is great because it frees both hands to handle a ribbon as it is coming off the blade. It is easy to get careless if you use a foot pedal but the one time I have been seriously cut it was with a manual microtome so I guess the moral is to simply be careful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna Harclerode Sent: Tuesday, January 06, 2009 11:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Cutting Safety Begin forwarded message: > From: Donna Harclerode > Date: January 6, 2009 8:27:14 PM PST > To: histonet-requ...@lists.utsouthwestern.edu > Subject: Microtome Cutting Safety > > Anyone know of a paraffin microtome that you can section WITH the > knife guard in position? I have always used forceps to keep my fingers > safe, but some techs are harder to convince to do this . I figured if > anyone knows they would be on this list. > I tried the Leica RM2255 (both in automated and manual mode) and I > could sort of section a couple small blocks with the guard up, but it > was not going to work with anything except perfect processed small > blocks and not well for those. > > Any opinions on the safety automated versus manual microtomes? I > adore automated cryostats (Leica 3050 is my favorite) , but I can not > figure why use an automated paraffin microtome. Logically I always > figured an automated can do more damage, but really have no facts. > > Thanks in advance, > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Outside Lab
Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Outside Lab
The first question on everybody's mind is "How much are you charging them"? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Outside Lab
Go to http://www.cms.hhs.gov/center/asc.asp for more information about Ambulatory Surgical Centers (ASC's) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Outside Lab
Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, January 07, 2009 9:51 AM To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab The first question on everybody's mind is "How much are you charging them"? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Doublestain
I am supposed to try a doublestain using the DAKO autostainer where one of the antibodies requires a Proteinase K pretreatment and the other antibody requires antigen retrieval with heat. Should I do both, or will this not work at all? Thank you Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome Cutting Safety
Donna Leica makes a microtome series RM2200 that allows you to cut sections with the knife guard up. You will be able to decide whether you would like a rotary microtome or a automated microtome. I see that you like the Leica CM3050. The comparable microtome for paraffin cutting is the RM2255. Your request can be forwarded to a rep in your area if you like. I will need your zip code to do that. If you need any other information you are welcome to contact me here at Leica. Best regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailh...@leica-microsystems.com www.leica-microsystems.com Donna Harclerode Sent by: To histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Microtome Cutting Safety 01/06/2009 10:43 PM Begin forwarded message: > From: Donna Harclerode > Date: January 6, 2009 8:27:14 PM PST > To: histonet-requ...@lists.utsouthwestern.edu > Subject: Microtome Cutting Safety > > Anyone know of a paraffin microtome that you can section WITH the > knife guard in position? I have always used forceps to keep my > fingers safe, but some techs are harder to convince to do this . I > figured if anyone knows they would be on this list. > I tried the Leica RM2255 (both in automated and manual mode) and I > could sort of section a couple small blocks with the guard up, but > it was not going to work with anything except perfect processed > small blocks and not well for those. > > Any opinions on the safety automated versus manual microtomes? I > adore automated cryostats (Leica 3050 is my favorite) , but I can > not figure why use an automated paraffin microtome. Logically I > always figured an automated can do more damage, but really have no > facts. > > Thanks in advance, > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Outside Lab
Why does the hospital pathologist go to the Urology to read and sign out the cases? So if I am understanding this correctly, your hospital is right now getting the tech part of the specimen for processing. Is your pathologist part of the hospital or are they their own group? The urology group will bill for their own tech part and make money from that part of the service which they are not currently making revenue from. I would assume that your pathologist are their own group and they don't care who makes the tech part? This is just business and you will have to make your services and prices competitive enough to make it not profitable for the urology group. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, January 07, 2009 10:11 AM To: 'jstaruk'; 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, January 07, 2009 9:51 AM To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab The first question on everybody's mind is "How much are you charging them"? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Outside Lab
The pathologists are probably going to the urology group to read because CLIA certification is LOCATION specific. The urology group may already have CLIA cert. and is splitting the interpretation charges with the pathologist. By adding its own lab, they will be able to capture both tech and interp charges. From: Mike Pence To: Bernice Frederick ; jstaruk ; "Senn, Amy R" ; Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 7, 2009 10:50:49 AM Subject: RE: [Histonet] Outside Lab Why does the hospital pathologist go to the Urology to read and sign out the cases? So if I am understanding this correctly, your hospital is right now getting the tech part of the specimen for processing. Is your pathologist part of the hospital or are they their own group? The urology group will bill for their own tech part and make money from that part of the service which they are not currently making revenue from. I would assume that your pathologist are their own group and they don't care who makes the tech part? This is just business and you will have to make your services and prices competitive enough to make it not profitable for the urology group. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, January 07, 2009 10:11 AM To: 'jstaruk'; 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, January 07, 2009 9:51 AM To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab The first question on everybody's mind is "How much are you charging them"? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now opening its own histology lab because it's more cost effective. Unless it's too early in the morning for us all to function, us histotechs can't figure out where they're saving money. We are being told that the Urology office (or any outside dr's office that has its own histo lab) can bill Medicare (or any insurance co.) differently, thereby making more money for the practice itself. Does anyone have any input on this? One of our pathologists is telling the Urology group NOT to do this because Medicare may change it's regulations again & need an 'established' lab to complete the work, instead of an 'up & coming' laboratory, so he's also against the Urology group setting up it's own lab. Thanks for everyone's 0.02. Have a great day!!! Amy Senn Histotech, Histology Laboratory Camp Hill, PA 17011 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ His
Re: [Histonet] IHC Doublestain
Any different of the two approaches? I think for most antigen they both work. 2009-01-08 TF 发件人： Kimberly Tuttle 发送时间： 2009-01-08 00:24:59 收件人： histonet@lists.utsouthwestern.edu 抄送： 主题： [Histonet] IHC Doublestain I am supposed to try a doublestain using the DAKO autostainer where one of the antibodies requires a Proteinase K pretreatment and the other antibody requires antigen retrieval with heat. Should I do both, or will this not work at all? Thank you Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] searching antibodies cdk4 and mdm2
Hi all! Can anybody recommand an anti-human-cdk4 and an anti-human-mdm2 antibody for FFPE tissue? We want to stain liposarcomas and use the Benchmark XT. Until now we have purchased cdk4 from NovusBiological (1:10, 60 min with amplifier) and mdm2 from proteintechgroup (1:50, 32 min). But the result are not very convincing. Any help is appreciated! Bye Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Outside Lab
Get used to this sort of thing. It is happening in urology and derm practices. You can not blame them for keeping it in-house. They are making a profit on it so why not do it yourself? On Wed, Jan 7, 2009 at 12:11 PM, Paula Pierce < cont...@excaliburpathology.com> wrote: > The pathologists are probably going to the urology group to read because > CLIA certification is LOCATION specific. The urology group may already have > CLIA cert. and is splitting the interpretation charges with the > pathologist. By adding its own lab, they will be able to capture both tech > and interp charges. > > > > > > From: Mike Pence > To: Bernice Frederick ; jstaruk < > jsta...@masshistology.com>; "Senn, Amy R" ; > Histonet@lists.utsouthwestern.edu > Sent: Wednesday, January 7, 2009 10:50:49 AM > Subject: RE: [Histonet] Outside Lab > > Why does the hospital pathologist go to the Urology to read and sign out > the cases? > So if I am understanding this correctly, your hospital is right now > getting the tech part of the specimen for processing. Is your > pathologist part of the hospital or are they their own group? The > urology group will bill for their own tech part and make money from that > part of the service which they are not currently making revenue from. I > would assume that your pathologist are their own group and they don't > care who makes the tech part? > > This is just business and you will have to make your services and prices > competitive enough to make it not profitable for the urology group. > > Mike > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice > Frederick > Sent: Wednesday, January 07, 2009 10:11 AM > To: 'jstaruk'; 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Outside Lab > > > Is the histo lab in the urology office CAP certified? > Bernice > > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > ECOGPCO-RL > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk > Sent: Wednesday, January 07, 2009 9:51 AM > To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Outside Lab > > The first question on everybody's mind is "How much are you charging > them"? > > Jim > > ___ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, > Amy R > Sent: Wednesday, January 07, 2009 8:28 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Outside Lab > > Hi histonetters, > > > > Hope everyone is having a good week so far... > > > > Our hospital receives specimens from a Urology group on a daily basis. > Our PA grosses the specimens here; us histotechs work our magic, and the > hospital pathologists take the slides directly to the Urology office to > read them and sign them out. If there are any special stains needed, we > do them here in the hospital. > > > > We are being told that the Urology group is now opening its own > histology lab because it's more cost effective. > > Unless it's too early in the morning for us all to function, us > histotechs can't figure out where they're saving money. We are being > told that the Urology office (or any outside dr's office that has its > own histo lab) can bill Medicare (or any insurance co.) differently, > thereby making more money for the practice itself. > > > > Does anyone have any input on this? One of our pathologists is telling > the Urology group NOT to do this because Medicare may change it's > regulations again & need an 'established' lab to complete the work, > instead of an 'up & coming' laboratory, so he's also against the Urology > group setting up it's own lab. > > > > Thanks for everyone's 0.02. > > > > Have a great day!!! > > > > > > > > > > Amy Senn > > Histotech, Histology Laboratory > > Camp Hill, PA 17011 > > > > > > Confidentiality Disclaimer: The information contained in this > communication may be confidential, is intended for the use of the > recipient named above, and may be legally privileged.If the reader of > this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication, or > any of its contents, is strictly prohibited. If you received this > communication in error, please resend this communication to the sender > and delete the original message and any copy of it from your computer > system. Thank You ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >
[Histonet] PTEN Protocol
Would anyone using the Dako concentrate PTEN (clone 6H2.1) on their Benchmark XT be willing to share their protocol? We are having difficulty with staining. If we get any staining, it's very weak. We've contacted Dako but they've had no advice. Our next step is contacting Ventana but we thought we'd check with you all first. Any assistance would be appreciated. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Outside Lab
As long as you have someone who is willing to read it! -Original Message- From: Histonet Alias [mailto:histonetal...@gmail.com] Sent: Wednesday, January 07, 2009 12:12 PM To: Paula Pierce Cc: Mike Pence; Histonet Subject: Re: [Histonet] Outside Lab Get used to this sort of thing. It is happening in urology and derm practices. You can not blame them for keeping it in-house. They are making a profit on it so why not do it yourself? On Wed, Jan 7, 2009 at 12:11 PM, Paula Pierce wrote: The pathologists are probably going to the urology group to read because CLIA certification is LOCATION specific. The urology group may already have CLIA cert. and is splitting the interpretation charges with the pathologist. By adding its own lab, they will be able to capture both tech and interp charges. From: Mike Pence To: Bernice Frederick ; jstaruk ; "Senn, Amy R" ; Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 7, 2009 10:50:49 AM Subject: RE: [Histonet] Outside Lab Why does the hospital pathologist go to the Urology to read and sign out the cases? So if I am understanding this correctly, your hospital is right now getting the tech part of the specimen for processing. Is your pathologist part of the hospital or are they their own group? The urology group will bill for their own tech part and make money from that part of the service which they are not currently making revenue from. I would assume that your pathologist are their own group and they don't care who makes the tech part? This is just business and you will have to make your services and prices competitive enough to make it not profitable for the urology group. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, January 07, 2009 10:11 AM To: 'jstaruk'; 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, January 07, 2009 9:51 AM To: 'Senn, Amy R'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Outside Lab The first question on everybody's mind is "How much are you charging them"? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Outside Lab Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them and sign them out. If there are any special stains needed, we do them here in the hospital. We are being told that the Urology group is now o
[Histonet] Microtome Cutting Safety
"I have always used forceps to keep my fingers safe, but some techs are harder to convince to do this..." Dear God in Heaven. Not only are Dumont forceps are more precise and delicate than fingers, but forceps don't bleed. I would never, ever allow anyone in my lab to use their fingers to pull ribbons off of the microtome. I may as well allow them to pipette concentrated HCl by mouth. Write S.O.P's. Then follow them and enforce them. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Wed, 7 Jan 2009 07:25:32 -0500 > From: j...@cdc.gov > To: erid...@cox.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Microtome Cutting Safety > CC: > > I use my fingers too, so no help there. > > But an automated microtome is great because it frees both hands to > handle a ribbon as it is coming off the blade. It is easy to get > careless if you use a foot pedal but the one time I have been seriously > cut it was with a manual microtome so I guess the moral is to simply be > careful. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartl...@cdc.hhs.gov > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna > Harclerode > Sent: Tuesday, January 06, 2009 11:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome Cutting Safety > > > > Begin forwarded message: > > > From: Donna Harclerode > > Date: January 6, 2009 8:27:14 PM PST > > To: histonet-requ...@lists.utsouthwestern.edu > > Subject: Microtome Cutting Safety > > > > Anyone know of a paraffin microtome that you can section WITH the > > knife guard in position? I have always used forceps to keep my fingers > > > safe, but some techs are harder to convince to do this . I figured if > > anyone knows they would be on this list. > > I tried the Leica RM2255 (both in automated and manual mode) and I > > could sort of section a couple small blocks with the guard up, but it > > was not going to work with anything except perfect processed small > > blocks and not well for those. > > > > Any opinions on the safety automated versus manual microtomes? I > > adore automated cryostats (Leica 3050 is my favorite) , but I can not > > > figure why use an automated paraffin microtome. Logically I always > > figured an automated can do more damage, but really have no facts. > > > > Thanks in advance, > > > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Windows LiveTM: Keep your life in sync. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t1_allup_howitworks_012009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] equation problem PLEASE help
Good afternoon, I am hoping someone out there will take pity on a "mathematically" challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome Cutting Safety
I use a fine tipped camel hair brush (sometimes wetted water) to ease the ribbon from the blade. It works great without any danger to the operator. The most that can happen is losing the end of the brush and ending up with a stick. It also prevents damaging the blade holder. On convincing your techs...it could help them develop fine motor movement/cordination if they truely want to develop their professional expertise. Beth Millerman, HT, SRA/SWC Stiefel Laboratories, Inc JR R Sent by: histonet-boun...@lists.utsouthwestern.edu Wed 07 Jan 2009 11:30 AM --- To cc Subject [Histonet ] Microtome Cutting Safety --- "I have always used forceps to keep my fingers safe, but some techs are harder to convince to do this..." Dear God in Heaven. Not only are Dumont forceps are more precise and delicate than fingers, but forceps don't bleed. I would never, ever allow anyone in my lab to use their fingers to pull ribbons off of the microtome. I may as well allow them to pipette concentrated HCl by mouth. Write S.O.P's. Then follow them and enforce them. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Wed, 7 Jan 2009 07:25:32 -0500 > From: j...@cdc.gov > To: erid...@cox.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Microtome Cutting Safety > CC: > > I use my fingers too, so no help there. > > But an automated microtome is great because it frees both hands to > handle a ribbon as it is coming off the blade. It is easy to get > careless if you use a foot pedal but the one time I have been seriously > cut it was with a manual microtome so I guess the moral is to simply be > careful. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartl...@cdc.hhs.gov > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna > Harclerode > Sent: Tuesday, January 06, 2009 11:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome Cutting Safety > > > > Begin forwarded message: > > > From: Donna Harclerode > > Date: January 6, 2009 8:27:14 PM PST > > To: histonet-requ...@lists.utsouthwestern.edu > > Subject: Microtome Cutting Safety > > > > Anyone know of a paraffin microtome that you can section WITH the > > knife guard in position? I have always used forceps to keep my fingers > > > safe, but some techs are harder to convince to do this . I figured if > > anyone knows they would be on this list. > > I tried the Leica RM2255 (both in automated and manual mode) and I > > could sort of section a couple small blocks with the guard up, but it > > was not going to work with anything except perfect processed small > > blocks and not well for those. > > > > Any opinions on the safety automated versus manual microtomes? I > > adore automated cryostats (Leica 3050 is my favorite) , but I can not > > > figure why use an automated paraffin microtome. Logically I always > > figured an automated can do more damage, but really have no facts. > > > > Thanks in advance, > > > > Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC > > __
[Histonet] RE: equation problem PLEASE help
Hi Eva. Here’s how I would do it. (1 mole/1064 grams)*(.001 gram/ml)= 9.4 X 10^-7 mole/ml Concentration 1*Volume 1= Concentration 2 * Volume 2, or C1V1=C2V2 C1= 9.4 X 10^-7 mole/ml C2= 10X 10^-9 mole/ml V2= 2 ml Solving for V1... V1= (C2V2)/C1 V1= (10 X 10^-9 mole/ml*2.0 ml)/ 9.4 X 10^-7 mole/ml=.021 ml You need about 21 microliters of stock solution to make 2 mls of working solution. Use a calculator or Excel to get as many decimal places as you need. Jerry Ricks Research Scientist University of Washington Department of Pathology > From: e...@georgetown.edu > To: histonet@lists.utsouthwestern.edu > Date: Wed, 7 Jan 2009 16:46:23 -0500 > Subject: [Histonet] equation problem PLEASE help > > Good afternoon, > I am hoping someone out there will take pity on a "mathematically" challenged > individual such as myself. I have been trying for hours to wrap my head > around this equation and am now getting to the point where I am more confused > than ever. Please help me. > The protocol calls for 10nmol of a substance per 1ml needed. It comes in a > 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If > for example I needed 2ml of the solution... > The clearer the explanation the better. I really want to understand the > calculation not just have an answer. PLEASE HELP ME. > Thank you, > Eva > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Windows LiveTM Hotmail®: Chat. Store. Share. Do more with mail. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t1_hm_justgotbetter_explore_012009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat safety question
The discussion on microtome safety begs me to ask a cryostat question We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat safety question
Hi Andrea, It's great to hear that you are happy with your cryostat and, hopefully, I'll be able to help you with an answer to your question. Leica sells a small device called a Thermal Block that is designed to help you remove the specimen from the chuck without causing it to melt. The catalog number is 14039818542. It's a small, mobile device that can be used in any of our cryostats that use chucks with stems in the back (we have a different one for the new CM1950 flat backed chucks). If you have questions or would like additional information, please feel free to contact our Technical Applications Center (1-800-248-0123 option 1 then option 2). Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! "Andrea Hooper" To Sent by: Histonet histonet-bounces@ lists.utsouthwest cc ern.edu mari.ann.mailh...@leica-microsystem s.com Subject 01/07/2009 05:40 [Histonet] Cryostat safety question PM The discussion on microtome safety begs me to ask a cryostat question We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat safety question
Andrea: I work in a Mohs clinic where all we cut is frozen skin sections. Needless to say, we don't have 50 chucks laying around... In the morning before clinic starts we put a layer of freezing medium on chucks and put them in the cryostat to freeze. When we get specimens, we add another drop or so to the already frozen 'button' and immediately embed the tissue in it. We usually add another small drop on top after it has begun to freeze, to cover the specimen completely. Cut as normal when frozen. After done cutting all you have to do is use a forceps or other blunt object and pop the bit with the specimen in it away from the 'button' and return the chuck to the cryostat and it can be reused the rest of the day. The specimen is therefore still frozen for storage, and it has a quicker TAT. Plus you won't need nearly so many chucks, as they can be recycled almost as soon as you are done cutting. I usually keep 6-8 'buttons' in my cryostat, and our clinic can process up to 50 separate specimens a day. A word of caution. If your work area is humid sometimes a thin layer of frost can form on the surface of the 'button' and when you attempt to take sections the bit with the tissue will pop off the 'button'. All you need to do is add another drop of medium to the button and 'glue' the two back together. If you are going a while between cutting sessions, I usually store my 'buttons' upside(mountant side) down on one of the cryostat surfaces. It doesn't seem to develop the frost layer. Useful if you have tiny specimens. Hope my verbose explanation is helpful. Feel free to e-mail if you have any questions or are confused about my explanation. Claire Ingles Madison WI From: histonet-boun...@lists.utsouthwestern.edu on behalf of Andrea Hooper Sent: Wed 1/7/2009 5:40 PM To: Histonet Cc: mari.ann.mailh...@leica-microsystems.com Subject: [Histonet] Cryostat safety question The discussion on microtome safety begs me to ask a cryostat question We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat safety question
I have the same cyostat, but it doesn't matter which one you use for removing tissue from the chuck is a separate issue. Others may beg to differ from my technic, but I remove the block from the cryostat and set it on a paper towel for a couple of mintes. I do not thaw tissue, just soften it. I take a scapel or small weigh spatula and gently separate it off the chuck. With a thin layer of OCT in the plastic mold, I return the block back to its original labeled mold, wrap with aluminum foil, place in a ziploc, and store it back to the -80C. This is quick and limits exposure to higher temps. I have used blocks multiple times, especially for cross reactivity studies and never had a problem. I am very careful and need reusing the block for IHC receptor studies. Good luck. This is pretty easy and you can move on to the next block at the same time. Beth Millerman/SWC Senior Research Associate Stiefel Laboratories, Inc "Andrea Hooper" Sent by: histonet-boun...@lists.utsouthwestern.edu Wed 07 Jan 2009 03:40 PM --- To Histonet cc mari.ann. mailhiot@ leica-mic rosystems .com Subject [Histonet ] Cryostat safety question --- The discussion on microtome safety begs me to ask a cryostat question We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
R: [Histonet] equation problem PLEASE help
Your final solution: 1ml. will contain 10^-8 mole Its molarità will be: M = (10^-8 * 10^3)/1 = 10^-5 (mole / litre) Starting solution: 1ml. contains 10^-3/1064 = 9.40*10^-7 mole So you have to take: 1 : (9.40*10^-7) = Xml : 10^-5 Xml = (10^-5) / (9.40*10^-7) = 10,64 ml. of starting solution and bring them to 1 litre by adding 989.36 ml. of distilled water. Best Regards, Massimo Tosi --- Mer 7/1/09, e...@georgetown.edu ha scritto: Da: e...@georgetown.edu Oggetto: [Histonet] equation problem PLEASE help A: histonet@lists.utsouthwestern.edu Data: Mercoledì 7 gennaio 2009, 22:46 Good afternoon, I am hoping someone out there will take pity on a "mathematically" challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome Cutting Safety
I guess I wasn't totally clear in my earlier reply. I use my bare fingers but I also use a camel hair brush as Beth states. I direct the brush with my right hand and pick up the first part of the ribbon with my left. The brush is used to hold the last part of the ribbon to aid in maneuvering to the waterbath. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Beth Millerman Sent: Wed 1/7/2009 4:53 PM To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Cutting Safety I use a fine tipped camel hair brush (sometimes wetted water) to ease the ribbon from the blade. It works great without any danger to the operator. The most that can happen is losing the end of the brush and ending up with a stick. It also prevents damaging the blade holder. On convincing your techs...it could help them develop fine motor movement/cordination if they truely want to develop their professional expertise. Beth Millerman, HT, SRA/SWC Stiefel Laboratories, Inc JR R Sent by: histonet-boun...@lists.utsouthwestern.edu Wed 07 Jan 2009 11:30 AM --- To cc Subject [Histonet ] Microtome Cutting Safety --- "I have always used forceps to keep my fingers safe, but some techs are harder to convince to do this..." Dear God in Heaven. Not only are Dumont forceps are more precise and delicate than fingers, but forceps don't bleed. I would never, ever allow anyone in my lab to use their fingers to pull ribbons off of the microtome. I may as well allow them to pipette concentrated HCl by mouth. Write S.O.P's. Then follow them and enforce them. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Wed, 7 Jan 2009 07:25:32 -0500 > From: j...@cdc.gov > To: erid...@cox.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Microtome Cutting Safety > CC: > > I use my fingers too, so no help there. > > But an automated microtome is great because it frees both hands to > handle a ribbon as it is coming off the blade. It is easy to get > careless if you use a foot pedal but the one time I have been seriously > cut it was with a manual microtome so I guess the moral is to simply be > careful. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartl...@cdc.hhs.gov > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna > Harclerode > Sent: Tuesday, January 06, 2009 11:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome Cutting Safety > > > > Begin forwarded message: > > > From: Donna Harclerode > > Date: January 6, 2009 8:27:14 PM PST > > To: histonet-requ...@lists.utsouthwestern.edu > > Subject: Microtome Cutting Safety > > > > Anyone know of a paraffin microtome that you can section WITH the > > knife guard in position? I have always used forceps to keep my fingers > > > safe, but some techs are harder to convince to do this . I figured if > > anyone knows they would be on this list. > > I tried the Leica RM2255 (both in automated and manual mode) and I > > could sort of se
Re: [Histonet] equation problem PLEASE help
Hello Eva Put simply 1nmol is 10-9 moles Therefore 10nmol is 10-8 moles 1 mole = 1,064g Therefore 10-8 moles = 0.1064gor 0.01064mg Therefore your working solution of 10nmol/ml = 0.01064mg/ml Your stock solution is 1mg/ml Therefore your dilution factor is 1.0 = 94 (93.984962 exactly) 0.01064 Therefore you can add 1ml stock to 93ml of diluent to get your desired concentration.. I hope this is useful regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au >>> 8/01/2009 7:46 am >>> Good afternoon, I am hoping someone out there will take pity on a "mathematically" challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] equation problem PLEASE help
Hello Eva Unfortunately some of the script did not transfer in the original mail and 10-9 should read 10 to the minus 9 as the superscript was lost. The same for 10-8. Also there should have been a division line between 1.0 and 0.01064 in the calculation. I hope this helps. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au >>> "Anthony Reilly" 8/01/2009 12:39 pm >>> Hello Eva Put simply 1nmol is 10-9 moles Therefore 10nmol is 10-8 moles 1 mole = 1,064g Therefore 10-8 moles = 0.1064gor 0.01064mg Therefore your working solution of 10nmol/ml = 0.01064mg/ml Your stock solution is 1mg/ml Therefore your dilution factor is 1.0 = 94 (93.984962 exactly) 0.01064 Therefore you can add 1ml stock to 93ml of diluent to get your desired concentration.. I hope this is useful regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au >>> 8/01/2009 7:46 am >>> Good afternoon, I am hoping someone out there will take pity on a "mathematically" challenged individual such as myself. I have been trying for hours to wrap my head around this equation and am now getting to the point where I am more confused than ever. Please help me. The protocol calls for 10nmol of a substance per 1ml needed. It comes in a 1mg/ml solution and has a molecular weight of 1064g/mol. How do I do this? If for example I needed 2ml of the solution... The clearer the explanation the better. I really want to understand the calculation not just have an answer. PLEASE HELP ME. Thank you, Eva ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
