[Histonet] staining for manganese
Hi everybody, I have some fish specimens which have been exposed to toxic levels of manganese. The researcher is hoping to see manganese localised on the gill. Lillie mentions the use of benzidine to stain for manganese. I will try DAB, but my searching on the net has drawn a blank. Gerard Spoelstra veterinary histology Murdoch University Western Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome alignment
We use the microtome alignment instrument, which can be purchased from several companies. It is in our weekly checklist that all microtomes have to be adjusted (checked) with the microtome aligner. All microtomes are cut at the same angle also! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LFB on GMA sections
Hi all, Can anyone tell me if LFB stain works well for staining nerves in GMA sections? My pathologist is looking at sections of these stained elsewhere and she does not feel the myelin is staining as it should. Any insight on this particular combination? Thanks in advance. Carol Carol Wilson, HT(ASCP) Lead Technician/Histology Ricerca Biosciences, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sectioning on different microtomes
I'm on the same page as you Jenaine- we get blocks from all over so we are constantly rearranging the block holder. We also do have a histocollimeter to get the Microms back in line (it doesn't fit on the Leicas. Berncie Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Saturday, January 24, 2009 6:00 PM To: arvidsonkris...@yahoo.com; histonet Subject: RE: [Histonet] Sectioning on different microtomes I simply align my block holder to match up with the block. We get many outside blocks so I have learned to be pretty good at this. From: histonet-boun...@lists.utsouthwestern.edu on behalf of kristen arvidson Sent: Sat 1/24/2009 7:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned test paraffin block method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: CPT coding during accession
Well said Bob! Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Saturday, January 24, 2009 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: CPT coding during accession Tere Hodges at St. Mary's Hospital in Tucson, Arizona asks: When you put in charges as a preliminary charge ... do you over bill or under bill? I have always stayed under while putting in charges and then audit the doctor's charges the next day. Example: colon segment other then tumor... bill 88307 the next day tumor was found now it is bumped up to 88309 In my experience in a number of institutions, doing the CPT coding when you accession the specimen is a bad idea - most of the coding errors never get caught. CPT coding should be done at sign-out or afterward. Remember that a number of common skin lesions are 88304 rather than 88305 depending on the microscopic diagnosis, and that coding a hysterectomy specimen is a dark art that I don't like to make anybody else take responsibility for (except maybe Harry Potter, who after all can cast a patronus charm). I think the responsible pathologist should do the coding (this is a common practice, but not common enough), and that somebody else (like you) should check the pathologist's coding to make sure that nothing has been omitted (decalcification, the routine special stain that was useless in a particular case). Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ISH probes
Hello Histonetters! We are finding ourselves coming short on the time remaining that we can offer ISH Probes through Ventana. We have not heard when or if ISH (In-situ hybridization) will be offered any more through them. Does anyone out there know of other companies that would offer ISH? Thank you for your input. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Mexico Society for Histology
The Board of Directors of the NMSH met on Saturday to discuss the future of the Society. Because membership has declined to less than 10% of eligible members despite concerted efforts over the past five years to increase interest and participation, the decision was made to inactivate the Society. This decision was not reached without serious consideration and although the inactivation of the Society was not the decision we had hoped to make, the declining membership and participation made this move necessary. We will encourage our members to continue membership in NSH and in adjoining State Societies. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ISH probes
There's a wonderful company called Oligo's, Etc. (Wilsonville, OR) that synthesizes very affordable oligoprobes. But you have to supply the sequence information. Susan - Original Message - From: Sharon Campbell shar...@celligent.net To: histonet@lists.utsouthwestern.edu Sent: Monday, January 26, 2009 9:20 AM Subject: [Histonet] ISH probes Hello Histonetters! We are finding ourselves coming short on the time remaining that we can offer ISH Probes through Ventana. We have not heard when or if ISH (In-situ hybridization) will be offered any more through them. Does anyone out there know of other companies that would offer ISH? Thank you for your input. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] staining for manganese
Gerard: Many minerals can be seen in tissues as an unspecific black particles when reacting with reagents like ammonium sulfide in the Timm's procedure for copper in Wilson's disease cases. This method requires treating the sections with 0.1N HCl after the reaction to eliminate iron and zinc sulfides that may have been formed. In the same way asbestos can either be detected by ultra filtration of the macerated lung tissue or using the Pearl's iron reaction that will stain the iron contents of the proteinic asbestos bodies formed around the non-reactive asbestos fibers. I do not know of any specific reaction for manganese in tissues, although I think that the Timm's method without the acid treatment may reveal an unspecific reaction. If that occurs my advise will be to chemically determine the presence of manganese in positively reacting gills against non reactive gills from other animals as controls. In any event my advise is to avoid the use of benzidine, or to use it with extreme caution because it is a very well known carcinogen, as DAB is also. Sorry I cannot be more helpful. René J. --- On Mon, 1/26/09, Gerard Spoelstra g.spoels...@murdoch.edu.au wrote: From: Gerard Spoelstra g.spoels...@murdoch.edu.au Subject: [Histonet] staining for manganese To: histonet@lists.utsouthwestern.edu Date: Monday, January 26, 2009, 4:58 AM Hi everybody, I have some fish specimens which have been exposed to toxic levels of manganese. The researcher is hoping to see manganese localised on the gill. Lillie mentions the use of benzidine to stain for manganese. I will try DAB, but my searching on the net has drawn a blank. Gerard Spoelstra veterinary histology Murdoch University Western Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sectioning on different microtomes
There are microtome aligners that can help. They are available for Leica, Microm and Shandon Finesse. We used to use them here before we got all of the same microtomes. They worked pretty well. We purchased ours from Newcomer Supply. They're super easy to use and take lots less time than the paraffin block method. Hope this helps. Jodie Robertson, HT (ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Saturday, January 24, 2009 4:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned test paraffin block method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sectioning on different microtomes
I don't know how these aligning tools work, but I always used an inexpensive level from the hardware store. You need one that can read in the vertical position. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jodie Robertson wrote: There are microtome aligners that can help. They are available for Leica, Microm and Shandon Finesse. We used to use them here before we got all of the same microtomes. They worked pretty well. We purchased ours from Newcomer Supply. They're super easy to use and take lots less time than the paraffin block method. Hope this helps. Jodie Robertson, HT (ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Saturday, January 24, 2009 4:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned test paraffin block method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: staining for manganese
Hi, There is a Timm stain - like protocol from Angenstein (Manganese-enhanced MRI reveals structural and functional changes in the cortex of Bassoon mutant mice. Angenstein F, Niessen HG, Goldschmidt J, Lison H, Altrock WD, Gundelfinger ED, Scheich H. Cereb Cortex. 2007 Jan;17(1):28-36) using Na2S that shows some interesting results with manganese autometallography. The problem, as mentionned by RJ Buesa, concerns the specificity of the staining. Using this method in the past I observed staining increase in the brain following MnCl injection. Regards, Benoît Delatour -- Message: 1 Date: Mon, 26 Jan 2009 18:58:34 +0900 From: Gerard Spoelstra g.spoels...@murdoch.edu.au Subject: [Histonet] staining for manganese To: histonet@lists.utsouthwestern.edu Message-ID: cf0145ebf1eb4c4e82768d82886a0c9b8fb...@pluto.ad.murdoch.edu.au Content-Type: text/plain; charset=iso-8859-1 Hi everybody, I have some fish specimens which have been exposed to toxic levels of manganese. The researcher is hoping to see manganese localised on the gill. Lillie mentions the use of benzidine to stain for manganese. I will try DAB, but my searching on the net has drawn a blank. Gerard Spoelstra veterinary histology Murdoch University Western Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
