[Histonet] Equipment Maintenance
Can anyone recommend a good East Coast histo equipment maintenance company? Regards, Brad Bradley Rodriguez Histology Coordinator HSRL (Histo-Scientific Research Laboratories) 5930 Main Street Mount Jackson, VA 22842 540.477.4440 Fax: 540.477.4448 b...@hsrl.org www.hsrl.org ***Confidentiality Notice: This e-mail is strictly confidential and intended solely for the addressee. If you are not the intended recipient you must not use, disclose, or copy this transmission. If you have received this message in error, please contact the sender and delete the material from any computer, disc drive, diskette, or other storage device or media. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Mountant for GMA stained sections
I agree with Gayle. We put a weight on the coverslip until it dries to help keep it flat. Since all mounting media has some organic solvent like xylene or toluene it is hard to avoid them completely. We use DPX for everything here. Best Regards, Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Friday, February 06, 2009 9:46 AM To: 'Histonet'; ' Histonet messages replies' Subject: [Histonet] Mountant for GMA stained sections Let stained GMA sections air dry and mount with a permanent mounting media. Dipping in xylene tends to crack or cause the GMA to wrinkle since the plastic can't be removed. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Opinions and any problems with Leica IPC
Hi, I'd be really grateful for feedback from small labs using a Leica IPC on consistency, and in problems. Thanks Steve ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Paraffin Blocks
We have so many malignant patients here that a week after I discarded blocks through 1984 (this was several years ago), I had a patient call for a block. When she said, Well, I just wasn't supposed to live this long I just cried. All I could do was apologize but I don't want to have to tell patients that any more! We have been sending out KRAS, BRAF and EGFR mutations on cases from the 90s just this past week - we can hardly keep up there are so many requests for these new tests. And I expect it will only increase just like Bob said..more of my 2 cents!!! j From: Bonner, Janet [mailto:janet.bon...@flhosp.org] Sent: Friday, February 06, 2009 8:50 AM To: Weems, Joyce; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Paraffin Blocks We are a very large facility and kept the majority of our blocks and slides in a minimally air-conditioned hospital supplies warehouse. I used to pull blocks and slides for legal cases, and it was rare-rare-rare that anyone did anything with the blocks after five years. Ten years for the slides was appropriate. Janet Janet L. Bonner, HTL (ASCP) Pathology Laboratory From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Thu 2/5/2009 6:12 PM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Paraffin Blocks I've been preaching this sermon for several years!!! We must do something!!! j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, February 05, 2009 5:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Paraffin Blocks How long to retain paraffin blocks (human tissue) is an issue that's going to need re-thinking. Presently most pathology services in the USA retain them for no more than ten years. If the laboratory closes, slides and blocks are disposed of immediately. It seems to me that in the coming age of molecular pathology, there will be a lot more interest in recovering paraffin blocks after longer periods of time. I think that the regulatory agencies will soon need to address this problem. Storing present-day paraffin blocks, with their bulky plastic mounts and low-melting waxes, is going to become even more difficult than it is now, as hospitals and other facilities consider the cost of maintaining and cooling storage space. I don't think it's possible to triage blocks and slides for disposal at the time the case is signed out. It sounds like a good idea, but the few times I've seen it tried it didn't work. Also, we really don't know what we're going to need to recover in the future. Bob Richmond Samurai Pathologist (nearly 70, isn't going to have to worry about this problem) Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Equipment Maintenance
Southeast Pathology Instrument Services, Inc based out of Folly Beach, SC. Michael Dietrich is the BEST! And so are the guys that work for him!!! 843-588-2559 843-708-7090 Michael's cell # Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bradley Rodriguez Sent: Friday, February 06, 2009 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment Maintenance Can anyone recommend a good East Coast histo equipment maintenance company? Regards, Brad Bradley Rodriguez Histology Coordinator HSRL (Histo-Scientific Research Laboratories) 5930 Main Street Mount Jackson, VA 22842 540.477.4440 Fax: 540.477.4448 b...@hsrl.org www.hsrl.org ***Confidentiality Notice: This e-mail is strictly confidential and intended solely for the addressee. If you are not the intended recipient you must not use, disclose, or copy this transmission. If you have received this message in error, please contact the sender and delete the material from any computer, disc drive, diskette, or other storage device or media. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Disinfecting Cryostat
Can someone suggest a good universal method for disinfecting the Cryostat after each use? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 63, Issue 7
Ditto - We really like our Glas coverslipper and LOVE their coverslipping media. Its perfect for their machine, and dries very quickly. We usually file in 2 days time, air dried only. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor 2. RE: Mounting media question (Molinari, Betsy) I use Sakura Tissue Tek Glas mounting media. I can file the next day. Betsy Molinari HT(ASCP) -Original Message- Subject: [Histonet] Mounting media question We will soon be receiving our new Sakura Glas Coverslipper. Can anyone who is also using this instrument please tell me what type of mounting media is being used. We are looking for a fast-drying media that can be used for tissue slides, non-gyne and gyne slides. We are also looking at long term storage without fading capability. Our current mounting media - Surgipath's Micromount used on the older model Sakura -Glas coverslipper does not dry quickly. Storage and slide handling issues around this problem. Thank you, Karen J Kay, MLT Pathology Supervisor Chinook Health Laboratory Chinook Regional Hospital - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mountant for GMA stained sections
Let stained GMA sections air dry and mount with a permanent mounting media. Dipping in xylene tends to crack or cause the GMA to wrinkle since the plastic can't be removed. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Disinfecting Cryostat
I believe CAP recommends 70% etoh. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Hartson [louise_hart...@urmc.rochester.edu] Sent: Friday, February 06, 2009 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disinfecting Cryostat Can someone suggest a good universal method for disinfecting the Cryostat after each use? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Best Decal?
What is the best decal to use for bone such as knee femur? We are currently using Decalcifying H from Ever Scientific at the grossing area for 4 - 8 hours and Decal stat at the microtome for another 2 hours. It seems like there must be a better way? Both the Decal H and the Decal Stat are hydrochloric acid based. Cindy Dubois Integrated Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Paraffin Blocks
All We are a tissue bank and heavily involved with clinical trials. We are trying to find a way for people to flag cases on these trials that have consented to future use so that when it comes time for block disposal,those blocks are kept or sent to the appropriate group that ran and or banks the tissue for that trial. We have translational studies as far back as 1990 that I ma afraid the rest of the case is gone. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, February 05, 2009 5:13 PM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Paraffin Blocks I've been preaching this sermon for several years!!! We must do something!!! j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, February 05, 2009 5:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Paraffin Blocks How long to retain paraffin blocks (human tissue) is an issue that's going to need re-thinking. Presently most pathology services in the USA retain them for no more than ten years. If the laboratory closes, slides and blocks are disposed of immediately. It seems to me that in the coming age of molecular pathology, there will be a lot more interest in recovering paraffin blocks after longer periods of time. I think that the regulatory agencies will soon need to address this problem. Storing present-day paraffin blocks, with their bulky plastic mounts and low-melting waxes, is going to become even more difficult than it is now, as hospitals and other facilities consider the cost of maintaining and cooling storage space. I don't think it's possible to triage blocks and slides for disposal at the time the case is signed out. It sounds like a good idea, but the few times I've seen it tried it didn't work. Also, we really don't know what we're going to need to recover in the future. Bob Richmond Samurai Pathologist (nearly 70, isn't going to have to worry about this problem) Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Disinfecting Cryostat
We use 70% ethanol on gauze (just damp) to wipe down inside of cryostat. Do not use 95% or 100% alcohol, as 70% is the most effective for disinfection, commonly used in biohoods. We pick up little trimmings with first dampened gauze (or kim wipe) then re-wipe with a second gauze. We prefer gauze for its flexibility. Also, if you put a kimwipe or gauze behind knife holder to l catch trimmings then you can fold up kimwipe, and lift garbage out, intact, and go to biohazard container before wipe down. We try to avoid too much alcohol on knife holder parts since alcohol ruins lubricants needed for good operation. Check with your cryostat manufacturer to see what they recommend. It pays to wipe down under and over sliding glass door to counterattack biohazardous cling-ons found on your gloves, and where people have touched the instrument. 70% alcohol will not kill prions. Water based disinfectants freeze in cryostat. Eventually you need scheduled disinfection with cryostat in defrosted mode with approved disinfecting agent. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Hartson Sent: Friday, February 06, 2009 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disinfecting Cryostat Can someone suggest a good universal method for disinfecting the Cryostat after each use? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Flammable storage
How does everyone store any reagents that need refrigeration that are also flammable? We have always just stored them in the routine histology refrigerator. I have never had this concern come up before and the charge tech of toxicology is now questioning this type of storage. Any guidance on this would be greatly appreciated!! Thanks, as always fellow histonetters, the best group in the world!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Flammable storage
We have purchased a flammable or explosion proof refrigerator from Fisher Scientific. The thermostat is on the outside of the cabinent not inside the cabinet. This is very important for two reasons: 1) if you have flammable or ignitable reagents and you store then in a frost free refrigerator (regular Sears , etc refrigerator) you will run the risk of an explosion or fire. We had this happen here at UAMS several years ago when some put a flammable reagent in a regular freezer. When the compressor came on it blue the door off of the refrigerator and set the whole lab on fire. You do not want to deal with this. 2) If you store your antibodies, kits, etc in a frost free or regular refrigerator it will destroy the antibody because a frost free refrigerator cycles and in cycling will heat up and make your antibody expire quicker. I know that these special refrigerators are very expensive but if you have a lot of antibodies and kits, or if you have a lot of flammables that must be kept cold it is worth it. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, February 06, 2009 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flammable storage How does everyone store any reagents that need refrigeration that are also flammable? We have always just stored them in the routine histology refrigerator. I have never had this concern come up before and the charge tech of toxicology is now questioning this type of storage. Any guidance on this would be greatly appreciated!! Thanks, as always fellow histonetters, the best group in the world!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Best Decal?
Hi Cindy: We use 5% Formic Acid with a chemical end point test to determine when decalcification has been completed. We make our own 5% Formic Acid but Newcomer's Supply has some that I have used which worked really well. If you have any questions please feel free to contact me. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Friday, February 06, 2009 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best Decal? What is the best decal to use for bone such as knee femur? We are currently using Decalcifying H from Ever Scientific at the grossing area for 4 - 8 hours and Decal stat at the microtome for another 2 hours. It seems like there must be a better way? Both the Decal H and the Decal Stat are hydrochloric acid based. Cindy Dubois Integrated Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] feulgen stain on brain sections
Hi all, i amusing Feulgen staining to visualize the apoptotic cells on 20 um brain frozen sections. The procedure is 5N HCl room temperature for 1.5 hour, wash in cool 1N HCl, wash in water, Schiff staining room temperature 1 h, wash throughly in distilled water, counterstain with 1% light green. I have two questions here: (1) after schiff staining, i can not visualize individual nucleui clearly, but all the section seems to be stained red. (2) I use distilled water to prepare 1% light green, am I right? I can not counterstain the section well - or, possibly because the sections are still in red color. 2009-02-07 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
