[Histonet] Moh's techniques
Good morning all, I'm learning a new way to do Moh's. Much more relaxed compared to how I did them years ago with a Pathologist looking over my shoulder while I attempted to cryosection 12, 3,9,12 o'clock boarders, stain them by hand and the Dermatologist wanting the results yesterday. I'd like to get some ideas as to techniques Moh's Techs are using out there that work well in assuring that one gets the entire skin edge. I'd also Like to shadow in any fairly local Moh's labs in the So.WI or No. Ill. area. Thanks everyone, Steve ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histo wrap
Obex. Jodie Robertson, HT (ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Inman, Anna Sent: Tuesday, February 24, 2009 5:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histo wrap Does anyone have a vendor for Histo Wrap - we have found this to be the best in terms of filtering? Thank you in advance Anna anna.in...@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Inflammation cell marker on Tissue Quantitive Immunohistofluorscence
I have a few suggestions that could help. I am interested in both inflammation and fibrosis in liver and have looked for markers of these two pathologies in several different ways. You say you have used ED-1, this suggests to me that you are likely staining rat liver. If you ever use mouse, the absolute best marker for Kupffer cells, liver resident macrophages, is F4/80. I have been using an antibody for F4/80 from Serotec for many years with wonderful results. For assessing neutrophils, I would suggest two different methods, IHC using a NIMP-R14 Ab (AbCAM) as well as a histochemical stain for chloracetate esterase. To get a bulk assessment of total leukocytes in the liver, you could try CD45 (CLA, common leukocyte antigen). This will not break down the various immune subsets, but it certainly can tell you if there is an increase in total leukocytes (as one would expect during inflammatory response). If you would like me to send you a list of markers for specific lymphocyte subsets, please let me know and I will be happy to furnish you with some ideas. However, when most people think inflammation, the first cells they think of are neutrophils...and then, of course, macrophages. ICAM (intracellular adhesion molecule)-1 is upregulated on hepatic sinusoidal endothelium with inflammation, and it thought to play an important role in extravasation of neutrophils from hepatic sinusoids into the parenchyma. I have used an ICAM-1 Ab from RD systems to do this (in mice). We routinely perform TNFa IHC in liver. Strikingly, we find that hepatocytes as well as Kupffer cells can produce TNFa. I can give you info on the Ab we use for this immunofluorescence (IF) if you would like. As far as quantification goes, our lab quantifies TNFa IF using ImagePro software and uses intensity of staining. It is also possible to quantify individual fluorescent cells with ImagePro. Simply speaking, you 'educate' (program) the software to count for you. You can achieve this by telling the program what colour pixels = positive. This program will also allow you to determine intensity of each individual spot if you would like; I don't know if ED-1 expression changes with activation of macrophages, so I don't know if intensity measures would help you or not. With respect to the quantification, once counted, you can edit what it counted to remove non-specific staining that happened to fall within the fluorescent parameters you set. For example, sometimes, a speck of non-cell associated fluorophore shows up, I remove these spots. To help in this regard, DAPI staining is used; if any given cell contains a nucleus and my marker of interest, I count it...those random fluorophore bits are anuclear which gives me rational to untag them. This is a *MUCH* faster way to enumerate cells than counting by hand. I hope this helps, if you have further questions, concerns, want additional details, please feel free to contact me. I could discuss this for days but don't want to bore you if I am not addressing your specific questions. Kind regards: ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: prit...@ccf.org Lab location: Lerner Research Institute NE4-214 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of TF Sent: Wednesday, February 25, 2009 12:48 AM To: histonet Subject: [Histonet] Inflammation cell marker on Tissue Quantitive Immunohistofluorscence Dear All: Just wonder any one have the experience to work on inflammation on different tissues, especially Liver, SKIN, and Brain? We want to look at wound-resulted inflammation level in these tissues, using immunohistochemical techniques rather RT-PCR / WB on cytokine levels. Can anyone recommend different cell markers for these tisses, separately? Another question is how about the Quantitive Immunohistofluorscence. I have some sections stained with Ed-1, marker of macrophage. It is very hard to count the number of positive cells ...can we use immunofluorescence instensity (same exposure time) or the area of positive region as the quantitive index? At least semi-qunatitive. 2009-02-25 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain
RE: [Histonet] RE: testing cutting ability--This is starting toReally Annoy me.
Come on, children - it's now time-out for this topic Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 25, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu; JR R Subject: Re: [Histonet] RE: testing cutting ability--This is starting toReally Annoy me. Instead of being so offended, why you just try to find out how different is academia from an AP histology lab? René J. --- On Mon, 2/23/09, JR R rosenfeld...@hotmail.com wrote: From: JR R rosenfeld...@hotmail.com Subject: [Histonet] RE: testing cutting ability--This is starting to Really Annoy me. To: histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 2:58 PM All this talk of having people actually section as part of the interview process is offensive to me. I have hired and trained lots of histotechnologists. Mostly I train them myself, from scratch, but sometimes I hire them pre-trained. Then of course, I re-train them anyway so they can do the work to the specs of my lab. Any deficiencies they have will be corrected through training. By me. At my University there is a formal 30 day (maybe it's longer, I can't recall) probationary period for new hires. That's good enough. Why in the world would I need to have them section as part of an interview process? I am also offended by the blocks per hour language. Partly it's because in my lab we do exhaustive serial sectioning, so I consider a tech who gives me one block every half-hour to be doing a good job. Maybe it's different in clinical work, but...20 blocks per hour? And you expect quality work? Jeez, are clinical histopath labs just sweat-shops? It's no wonder histopathologists feel overworked and underappreciated. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Mon, 23 Feb 2009 10:14:06 -0500 From: trathbo...@somerset-healthcare.com To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; tbr...@holyredeemer.com Subject: RE: [Histonet] RE: testing cutting ability during an interview CC: We also have the staff talk to the applicant during this process. It gives you a good idea if the person can talk and cut at the same time. The last thing anyone wants is a histotech that has to stop sectioning during a conversation! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Rene J Buesa Sent: Monday, February 23, 2009 10:05 AM To: histonet@lists.utsouthwestern.edu; Terri Braud Subject: Re: [Histonet] RE: testing cutting ability during an interview I for one always required any applicant to prepare 20 slides stained with HE. I can assure that I selected difficult blocks to cut and the applicant was required to sign a disclaimer that included that s/he he knew how to section and avoid injuries. The whole process was timed (to get a first idea about productivity) and I evaluated and graded the slides at the end. The results were used as one of the elements to decide about offering the position (the fundamental) but I waited until all the applicants had completed the tests so sometimes the applicant had to be contacted a few days later to let him/her know about the results. René J. --- On Mon, 2/23/09, Terri Braud tbr...@holyredeemer.com wrote: From: Terri Braud tbr...@holyredeemer.com Subject: [Histonet] RE: testing cutting ability during an interview To: histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 8:57 AM From a recent digest: If you want to know if someone you are interviewing can really section or stain, set them down at a microtome during the interview process, and watch them. I have a question about the following statement plucked from a recent digest. What are the legal ramifications if a person cuts themselves during an interview? We've had this discussion at my place of employment and came to the decision that it would leave us open to a legal liability. I would love to hear some discussion on this subject, as well as any experiences that others have had. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any
Re: [Histonet] RE: testing cutting ability--This is starting to Really Annoy me.
Instead of being so offended, why you just try to find out how different is academia from an AP histology lab? René J. --- On Mon, 2/23/09, JR R rosenfeld...@hotmail.com wrote: From: JR R rosenfeld...@hotmail.com Subject: [Histonet] RE: testing cutting ability--This is starting to Really Annoy me. To: histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 2:58 PM All this talk of having people actually section as part of the interview process is offensive to me. I have hired and trained lots of histotechnologists. Mostly I train them myself, from scratch, but sometimes I hire them pre-trained. Then of course, I re-train them anyway so they can do the work to the specs of my lab. Any deficiencies they have will be corrected through training. By me. At my University there is a formal 30 day (maybe it's longer, I can't recall) probationary period for new hires. That's good enough. Why in the world would I need to have them section as part of an interview process? I am also offended by the blocks per hour language. Partly it's because in my lab we do exhaustive serial sectioning, so I consider a tech who gives me one block every half-hour to be doing a good job. Maybe it's different in clinical work, but...20 blocks per hour? And you expect quality work? Jeez, are clinical histopath labs just sweat-shops? It's no wonder histopathologists feel overworked and underappreciated. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Mon, 23 Feb 2009 10:14:06 -0500 From: trathbo...@somerset-healthcare.com To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; tbr...@holyredeemer.com Subject: RE: [Histonet] RE: testing cutting ability during an interview CC: We also have the staff talk to the applicant during this process. It gives you a good idea if the person can talk and cut at the same time. The last thing anyone wants is a histotech that has to stop sectioning during a conversation! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Rene J Buesa Sent: Monday, February 23, 2009 10:05 AM To: histonet@lists.utsouthwestern.edu; Terri Braud Subject: Re: [Histonet] RE: testing cutting ability during an interview I for one always required any applicant to prepare 20 slides stained with HE. I can assure that I selected difficult blocks to cut and the applicant was required to sign a disclaimer that included that s/he he knew how to section and avoid injuries. The whole process was timed (to get a first idea about productivity) and I evaluated and graded the slides at the end. The results were used as one of the elements to decide about offering the position (the fundamental) but I waited until all the applicants had completed the tests so sometimes the applicant had to be contacted a few days later to let him/her know about the results. René J. --- On Mon, 2/23/09, Terri Braud tbr...@holyredeemer.com wrote: From: Terri Braud tbr...@holyredeemer.com Subject: [Histonet] RE: testing cutting ability during an interview To: histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 8:57 AM From a recent digest: If you want to know if someone you are interviewing can really section or stain, set them down at a microtome during the interview process, and watch them. I have a question about the following statement plucked from a recent digest. What are the legal ramifications if a person cuts themselves during an interview? We've had this discussion at my place of employment and came to the decision that it would leave us open to a legal liability. I would love to hear some discussion on this subject, as well as any experiences that others have had. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset
Re: [Histonet] Hi histoland, Anyone out there using canada balsam? If so where are you purchasing? Thanks in adva
We have a bottle of it here in the lab. It came from Acros, which means we ordered it from Fisher Scientific. Maggie On 2/25/09 11:30 AM, Janice Mitchell mitchel...@email.chop.edu wrote: Hi histoland, Anyone out there using canada balsam? If so where are you purchasing? Thanks in advance. Janice ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slidex filing pages
Hi all, Anyone familiar with Slidex pages - rigid pastic pages in which one can store slides (20/page)? These pages are pre-punched so they can go into ring binder but there is also a filing box that one can use. We have been using these for years, but the agency that dealt with the parent company has pulled out and we are getting no joy from Slidex Japan. Seems they don't answer English correspondence. Any thoughts?-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: testing cutting ability--This is starting to Really Annoy me.
Instead of being so offended, why you just try to find out how different is academia from an AP histology lab? René J. I believe that I have already described an understanding that research is different than clinical. I did actually start off as a nurse way back when. Human dignity is the same everywhere though. It sounds like clinical labs mostly treat their Histotechs like dogs--starting with the interview, and continuing throughout the Tech's career. And I find that offensive. Jerry Ricks Research Scientist University of Washington Department of Pathology --- On Mon, 2/23/09, JR R rosenfeld...@hotmail.com wrote: From: JR R rosenfeld...@hotmail.com Subject: [Histonet] RE: testing cutting ability--This is starting to Really Annoy me. To: histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 2:58 PM All this talk of having people actually section as part of the interview process is offensive to me. I have hired and trained lots of histotechnologists. Mostly I train them myself, from scratch, but sometimes I hire them pre-trained. Then of course, I re-train them anyway so they can do the work to the specs of my lab. Any deficiencies they have will be corrected through training. By me. At my University there is a formal 30 day (maybe it's longer, I can't recall) probationary period for new hires. That's good enough. Why in the world would I need to have them section as part of an interview process? I am also offended by the blocks per hour language. Partly it's because in my lab we do exhaustive serial sectioning, so I consider a tech who gives me one block every half-hour to be doing a good job. Maybe it's different in clinical work, but...20 blocks per hour? And you expect quality work? Jeez, are clinical histopath labs just sweat-shops? It's no wonder histopathologists feel overworked and underappreciated. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Mon, 23 Feb 2009 10:14:06 -0500 From: trathbo...@somerset-healthcare.com To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; tbr...@holyredeemer.com Subject: RE: [Histonet] RE: testing cutting ability during an interview CC: We also have the staff talk to the applicant during this process. It gives you a good idea if the person can talk and cut at the same time. The last thing anyone wants is a histotech that has to stop sectioning during a conversation! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Rene J Buesa Sent: Monday, February 23, 2009 10:05 AM To: histonet@lists.utsouthwestern.edu; Terri Braud Subject: Re: [Histonet] RE: testing cutting ability during an interview I for one always required any applicant to prepare 20 slides stained with HE. I can assure that I selected difficult blocks to cut and the applicant was required to sign a disclaimer that included that s/he he knew how to section and avoid injuries. The whole process was timed (to get a first idea about productivity) and I evaluated and graded the slides at the end. The results were used as one of the elements to decide about offering the position (the fundamental) but I waited until all the applicants had completed the tests so sometimes the applicant had to be contacted a few days later to let him/her know about the results. René J. --- On Mon, 2/23/09, Terri Braud tbr...@holyredeemer.com wrote: From: Terri Braud tbr...@holyredeemer.com Subject: [Histonet] RE: testing cutting ability during an interview To: histonet@lists.utsouthwestern.edu Date: Monday, February 23, 2009, 8:57 AM From a recent digest: If you want to know if someone you are interviewing can really section or stain, set them down at a microtome during the interview process, and watch them. I have a question about the following statement plucked from a recent digest. What are the legal ramifications if a person cuts themselves during an interview? We've had this discussion at my place of employment and came to the decision that it would leave us open to a legal liability. I would love to hear some discussion on this subject, as well as any experiences that others have had. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute
RE: [Histonet] Tube station
We do not allow formalin fixed specimens to be tubed. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Wednesday, February 25, 2009 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tube station We have just installed a tube station do you send your tissue specimens through the tube station ? Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plant Histology
Hi Histonetters: I was hoping someone could share a simple plant tissue (green stem and buds) processing program with me. I have never done plant histology, but would like to earn some extra credit for a horticulture class I'm taking. Thanks, Pam Plumlee H.T. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Mohs technique
First of all, there is no apostrophe in Mohs. Sorry, being picky is a necessity for a Mohs tech. How are you mounting your tissue? Are you using a glass slide to mount the tissue? There are several different method out there for mounting tissue so you can be sure the entire epidermis is completely down and air bubbles are out of the tissue. You might have your surgeon contact the Mohs College and see about getting a trainer to come and work with you. With your background, the 2 days the College suggests would work great. The other alternative is to go and visit a trainer. If you go onto the website www.mohscollege.org you can find a list of Mohs histotech trainers. Lynn Whitmore HT(ASCP) Mohs Histotechnology Trainer - -- Message: 17 Date: Wed, 25 Feb 2009 06:56:12 -0800 (PST) From: Steven Coakley sjchta...@yahoo.com Subject: [Histonet] Moh's techniques To: Histonet@lists.utsouthwestern.edu Message-ID: 164204.1533...@web38206.mail.mud.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Good morning all, I'm learning a new way to do Moh's. Much more relaxed compared to how I did them years ago with a Pathologist looking over my shoulder while I attempted to cryosection 12, 3,9,12 o'clock boarders, stain them by hand and the Dermatologist wanting the results yesterday. I'd like to get some ideas as to techniques Moh's Techs are using out there that work well in assuring that one gets the entire skin edge. I'd also Like to shadow in any fairly local Moh's labs in the So.WI or No. Ill. area. Thanks everyone, Steve -- M ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tube station
Ever since a leaky container and a biohazard bag opened in the tube containing bile- No Way. They had to shut the system down to clean it. And it went across the street and up 3 floors! University of Chicago has a dumbwaiter that sends samples from surgery to the gross room. Nifty!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Wednesday, February 25, 2009 1:27 PM To: 'Marcia Funk'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tube station We do not tube any tissue samples, fresh or fixed, and yes, we do have a tube station available to us. Potential contamination of the tube system from a leaking specimen container is one legitimate concern but we also have some here worried that a fresh specimen might enter the tube system never to be seen again. I'm not sure how realistic that fear is with modern systems that track each transaction however I'm reluctant to find out the hard way. Emotions aside, as a matter of policy we do not tube any tissues. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Wednesday, February 25, 2009 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tube station We have just installed a tube station do you send your tissue specimens through the tube station ? Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tube station
A tube station?! Is that like the internet--a series of connected tubes?! Emily On Wed, Feb 25, 2009 at 1:32 PM, Marcia Funk fu...@mercyhealth.com wrote: We have just installed a tube station do you send your tissue specimens through the tube station ? Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- It's like hearing Billy Joel play Piano Man-- joyless for all involved, but demanded by a higher power. --Kevin Murphy, Indiana Jones and the Crystal Skull rifftrax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Nuclear fast red
We have ordered Nuclear Fast Red from both EMS and Sigma and neither one of them seem to work very well. Both appear to have very weak staining. Is there someone out there in Histoland that could lead us to Nuclear Fast Red that actually works? Thanks is advance for all of your recommendations. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Nuclear fast red
Try Newcomer Supply. Jodie Robertson, HT (ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, February 25, 2009 1:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear fast red We have ordered Nuclear Fast Red from both EMS and Sigma and neither one of them seem to work very well. Both appear to have very weak staining. Is there someone out there in Histoland that could lead us to Nuclear Fast Red that actually works? Thanks is advance for all of your recommendations. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Nuclear fast red
Newcomer or American Master Tech -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, February 25, 2009 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear fast red We have ordered Nuclear Fast Red from both EMS and Sigma and neither one of them seem to work very well. Both appear to have very weak staining. Is there someone out there in Histoland that could lead us to Nuclear Fast Red that actually works? Thanks is advance for all of your recommendations. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] muscle skinning solution for fluorescence microscopy
I am in need of a good, working chemical skinnig technique for skeletal muscle fibers. I will be staining skinned, isolated fibrils with Rh-Phalloidin and DAPI stains for observation and analysis using a fluorescence scope. Thanks. - Matthew T. Close Lehigh University Department of Biological Sciences ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
