Re: [Histonet] microwave fixation
I go to the histonet because other techs have experience with different types of tissue and have tried everything to finally get the result.? Most vendors cannot offer?the same kind of advice and suggestions.? No offense but I have tried your microwave with another rep and it wasn't what I was looking for.? I am quite happy with the microwaves that I have, but am now processing other tissues in them.? I have used the histonet for years for advice and suggestions.? Because if you are having a problem with something or can't get something to work right, chances are someone on the histonet has been there and figured it out. That is the purpose of the histonet...to get advice from others when having a problem and to give advice when you have the answers. Roxanne -Original Message- From: Jes Strong j...@milestonemed.com To: godsgal...@aol.com Cc: Michelle Rederick m...@milestonemed.com Sent: Sat, 14 Mar 2009 11:17 am Subject: RE: [Histonet] microwave fixation Good Morning, I find it interesting that you continually go to the HistoNet for support on your microwave processor instead of the manufacturer of the unit. If you would be interested in upgrading to a Milestone microwave processor, we would be happy to demonstrate to you on site why we are the world leaders in that technology with 3 full time Histotechnicians, with many years of microwave processing experience, at your service to help you maximize your tissue processing. If you would be interested in trying out our microwave processors which were designed and built specifically for laboratory use, unlike the others which are converted kitchen microwave ovens, please let me know and we will be happy to put things in motion so that you can experience consistent high quality microwave processing. Please don't hesitate to contact me if you have any questions, Jes Strong Milestone Medical (847) 323-8373 j...@milestonemed.com www.milestonemed.com Helping Patients -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of godsgal...@aol.com Sent: Friday, March 13, 2009 2:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave fixation Me again! To those of you that do formalin fixation in the microwave, would you please share your protocol?? I am having issues Roxanne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 20 micron resin sections
Thanks all, This is more or less what i thought, that 20mu sections would prove to be difficult and not all that feasible. So, I wait with bated breath for the protocol from the researcher On 3/13/09, Peggy Bisher mbis...@princeton.edu wrote: One of the labs here use JB4 to section Zebrafish. I sent your question to them to see if they could help you out. Here is their response: The consensus in the lab (Kari and I) is that no, probably not. The size would probably shred the section and chip it to where they would be uneven, etc. Probably cryo or vibratome would be best. They routinely cut their sections between 2-5 microns. Good luck to you! Cheers, Margaret E. Bisher Electron Microscopy Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbis...@princeton.edu On 3/13/09 3:53 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I have a query from a colleague doing research on neuroanatomy as to whether it is possible ( with relative ease) to cut 20mu sections from JB4 resin embedded tissue? Apparently these sections ae to be stained and then used for stereomicroscopy. My experience is not that extensive to be able to answer her, so I would appreciate some advice here best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
