Re: [Histonet] processing v-e-r-y tiny samples
Shandon - or whatever they're called this week- sell nylon mesh biopsy bags. These are flat bags, sealed on 3 sides. I have found that if you cut one corner off (ie, a oblong piece with 2 sides still sealed) just a tiny bit bigger than the cassette, you can open the bag, like a book, insert the sample and flatten the bag over the specimen and place it snug and flat in the bottom of the casette. It sems to stay like that through processing (esp if kept the cassette is kept flat in the basket), and is easy to locate against the mesh for embedding. On 3/19/09, Andrea Grantham algra...@u.arizona.edu wrote: Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rehydration // urgent
Hi there, please help immediatly :) I want to rehydrate two specimen which have been processed as follows: 1h NBF 1h 70% 2-propanol (isopropanol) 1h 80% 2-propanol I need to go back to NBF as I want to add some more specimen but can process only one basket at one time (I'm using Microm STP120). Thanks for your replies! V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processing v-e-r-y tiny samples
Hi Andi, Happy Friday! I know there's been a Histogel backlash of late, but I would still recommend trying it. I haven't had any problems. We use it for scant neuro specimens every day. We also have used H.G. for mouse sural nerve biopsies that needed to retain orientation (very tiny). If you do use Histogel, do not put it in a histoscreen or biopsy cassette and be sure to put it on a process that's long enough. People often use the cassette/process based on the size of the tissue, not of the Histogel block. I'd ask thermo for a sample of H.G. and try it. If you need any Histogel help, let me know. I'm a huge fan! Have a great day, Jennifer Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -Original Message- From: Bryan Watson [mailto:bryan.wat...@parkview.com] Sent: Thursday, March 19, 2009 3:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra...@u.arizona.edu 3/19/2009 11:51 AM Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histogel
Wow, what is the histogrl backlash? Will someone please summarize the issues people are having? Thanks! Andrea -Original Message- From: Hofecker, Jennifer L jennifer.l.hofec...@vanderbilt.edu Date: Fri, 20 Mar 2009 08:23:02 To: histonet@lists.utsouthwestern.edu; Andrea Granthamalgra...@u.arizona.edu Subject: RE: [Histonet] processing v-e-r-y tiny samples Hi Andi, Happy Friday! I know there's been a Histogel backlash of late, but I would still recommend trying it. I haven't had any problems. We use it for scant neuro specimens every day. We also have used H.G. for mouse sural nerve biopsies that needed to retain orientation (very tiny). If you do use Histogel, do not put it in a histoscreen or biopsy cassette and be sure to put it on a process that's long enough. People often use the cassette/process based on the size of the tissue, not of the Histogel block. I'd ask thermo for a sample of H.G. and try it. If you need any Histogel help, let me know. I'm a huge fan! Have a great day, Jennifer Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -Original Message- From: Bryan Watson [mailto:bryan.wat...@parkview.com] Sent: Thursday, March 19, 2009 3:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra...@u.arizona.edu 3/19/2009 11:51 AM Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histogel
histogrl backlash thats a whole 'nother set of issues altogether ;) happy friday! anh2...@med.cornell.edu wrote: Wow, what is the histogrl backlash? Will someone please summarize the issues people are having? Thanks! Andrea -Original Message- From: Hofecker, Jennifer L jennifer.l.hofec...@vanderbilt.edu Date: Fri, 20 Mar 2009 08:23:02 To: histonet@lists.utsouthwestern.edu; Andrea Granthamalgra...@u.arizona.edu Subject: RE: [Histonet] processing v-e-r-y tiny samples Hi Andi, Happy Friday! I know there's been a Histogel backlash of late, but I would still recommend trying it. I haven't had any problems. We use it for scant neuro specimens every day. We also have used H.G. for mouse sural nerve biopsies that needed to retain orientation (very tiny). If you do use Histogel, do not put it in a histoscreen or biopsy cassette and be sure to put it on a process that's long enough. People often use the cassette/process based on the size of the tissue, not of the Histogel block. I'd ask thermo for a sample of H.G. and try it. If you need any Histogel help, let me know. I'm a huge fan! Have a great day, Jennifer Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -Original Message- From: Bryan Watson [mailto:bryan.wat...@parkview.com] Sent: Thursday, March 19, 2009 3:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra...@u.arizona.edu 3/19/2009 11:51 AM Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Employment Opportunity in Camp Hill, Pennsylvania
HISTOLOGY TECHNICIAN NEEDED! Holy Spirit Health System, a leading healthcare employer in South Central Pennsylvania, is currently seeking a full time Histology Technician. The ideal candidate will possess: * HT certification by Board of Registry of the American Society of Clinical Pathologists * Knowledge and understanding of histologic theory normally acquired through completion of one year at a certified school of histotechnology or through one year of on-the-job training * Strong interpersonal and communication skills * Full time, Day/Evening shift (12:00PM to 8:30PM) with some weekend rotation To apply, please visit our website at www.hsh.org http://www.hsh.org/ or email asch...@hsh.org for additional information. Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Intellipath Instrument
A colleague of mine is looking into purchasing an immuno stainer. Biocare has offered a good price on the Intellipath instrument, antibodies and reagents. While we know the antibodies are reagents are excellent, we know nothing about their instrumentation. Does anyone have any first hand experience with the Intellipath instrument, service from Biocare, etc...? Any information would be helpful. Thank you in advance. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PowerPath and Epic
Hi, we are in the process of moving to PowerPath replacing our current pathology computer system. Our hospital is using Epic and I am wondering if anyone has PowerPath that receives orders from Epic and sends results back. I am really interested in how orders are placed in Epic. Thanks Cordially, Pam ~ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkric...@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipients(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient(s), please contact the sender by return e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sure Path
Hey everyone, We are most probably going to be using Sure path in the future, in conjunction with Meditech. Can anyone give me any insight as to the pro's and con's of Sure Path and is it linkable to Meditech? Steve Clark Grand Strand Regional Medical Center Myrtle Beach, SC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Intellipath Instrument
Sheila, I have had the Intellipath since December and I love it. Any service issues have been immediately answered. Support has been above and beyond expectation. If you have any specific questions I will be happy to answer them. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Friday, March 20, 2009 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Intellipath Instrument A colleague of mine is looking into purchasing an immuno stainer. Biocare has offered a good price on the Intellipath instrument, antibodies and reagents. While we know the antibodies are reagents are excellent, we know nothing about their instrumentation. Does anyone have any first hand experience with the Intellipath instrument, service from Biocare, etc...? Any information would be helpful. Thank you in advance. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plastic storage containers for brain tissue?
Hi Everyone: We are looking for rectangular plastic containers to store our brain tissue in. We use two sizes, a 60 oz container (for fixed half brains or their slices) and a 128 oz container (for whole fixed brains or their slices). Recently we have been unable to locate a vendor that makes these sizes anymore. Does anyone know of a vendor that still makes these containers? Thanks, Tim Wheelock Harvard Brain Tissue Resource Center 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] dako autostainers
Hi Jen, Hope it's OK for a vendor to jump in here. I'm with IMEB, INC. We offer the Dako stainer refurbished with updated software. Units are completely refurbished and include warranty, installation and training. Please contact me if we can be of service! Margaret O'Donnell Sales Rep IMEB, INC Tel: 800-543-8496 Fax: 760-761-0859 marga...@imebinc.com The Pathology Specialists -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Thursday, March 19, 2009 8:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dako autostainers We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the older models anymore b/c they are worried that the software would be out of date. I looked into this on Dako.com and found that there is the older Dado Autostainer. After reading through its description it looks like it just lacks a few of the bells and whistles (like a slide labeler for example) that the Autostainer Plus has but, otherwise looks like it gets the job done just the same. Does anyone have any feedback on either one of these machines and whether or not you think I should even bother looking into the older model? Thank you so much. Jen C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Intellipath Instrument
We have also recently purchased this instrument. I highly recommend it. Sheila Haas micropathl...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 03/20/2009 09:28 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Intellipath Instrument A colleague of mine is looking into purchasing an immuno stainer. Biocare has offered a good price on the Intellipath instrument, antibodies and reagents. While we know the antibodies are reagents are excellent, we know nothing about their instrumentation. Does anyone have any first hand experience with the Intellipath instrument, service from Biocare, etc...? Any information would be helpful. Thank you in advance. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] For Sale small lab equipment
Dear Fellow Histonetters, I have 2 balances for sale 600 gm capacity, top load, each pass and are recertified every year balance weights for standarizing 3 Tissue Tek II staining trays and extra staining dishes and lots of Tissue Tek II staining racks 1 large glass dessicator 1 medium glass dessicator lots of consumables Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Brain containers
im Wheelock asks about brain containers: We use the 5.7 liter polyethylene containers from Richard Allen (Cat # 5357). When fixing whole brains we suspend the brain in the formalin in a hair net. We keep the brain slices in these containers too. Michael Titford USA Pathology Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] list of special stains
One of the histology text books??? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zerfas, Patricia (NIH/OD/ORS) [E] Sent: Thursday, March 19, 2009 5:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] list of special stains Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] list of special stains
DAKO has (or had as it's been some years since I got mine) a FREE Guide to Special Stains introductory manual, which was great for such info. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zerfas, Patricia (NIH/OD/ORS) [E] Sent: Thursday, March 19, 2009 5:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] list of special stains Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] automatic coverslipper
Hello everyone, Out lab is looking to getting an automatic coverslipper, we do about 6000 cases a year. What would be a good recommendation? Thanks Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processing v-e-r-y tiny samples
Careful - you may be close to the mark. Thermo laid off about 75 people here in Madison last week. Claire Shandon - or whatever they're called this week- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] list of special stains
I got one from our Dako rep a few weeks ago. They do still have this and it's free. Mark On Fri, Mar 20, 2009 at 11:13 AM, Andrea Hooper anh2...@med.cornell.eduwrote: DAKO has (or had as it's been some years since I got mine) a FREE Guide to Special Stains introductory manual, which was great for such info. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zerfas, Patricia (NIH/OD/ORS) [E] Sent: Thursday, March 19, 2009 5:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] list of special stains Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] automatic coverslipper
For your work volumen as well as for any other as well, get a Sakura film coverslipper. René J. --- On Fri, 3/20/09, zodia...@comcast.net zodia...@comcast.net wrote: From: zodia...@comcast.net zodia...@comcast.net Subject: [Histonet] automatic coverslipper To: histonet@lists.utsouthwestern.edu Date: Friday, March 20, 2009, 2:14 PM Hello everyone, Out lab is looking to getting an automatic coverslipper, we do about 6000 cases a year. What would be a good recommendation? Thanks Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] automatic coverslipper
The tape coverslipper from Sakura does work very well, but I would advise you to get buy in from your pathologists and anyone else that is going to be reading slides with the tape coverslips. Some people insist on glass coverslips. Tape coverslip advantages: 1) Speed (I have used one machine to coverslip 3-4,000 slides per day) 2) Quick drying (Important if you are a core lab and courier slides out somewhere) 3) Fast removal of coverslips (Several techniques are used, check the Histonet archives.) Glass Coverslip advantages 1) Saves tech time and reduces exposure to xylene. 2) Stays with traditional glass coverslip. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 214-237-1680 214-422-3083 Mobile 214-237-1706 FAX To learn more about ProPath, please visit http://www.ProPathLab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 20, 2009 2:45 PM To: histonet@lists.utsouthwestern.edu; zodia...@comcast.net Subject: Re: [Histonet] automatic coverslipper For your work volumen as well as for any other as well, get a Sakura film coverslipper. René J. --- On Fri, 3/20/09, zodia...@comcast.net zodia...@comcast.net wrote: From: zodia...@comcast.net zodia...@comcast.net Subject: [Histonet] automatic coverslipper To: histonet@lists.utsouthwestern.edu Date: Friday, March 20, 2009, 2:14 PM Hello everyone, Out lab is looking to getting an automatic coverslipper, we do about 6000 cases a year. What would be a good recommendation? Thanks Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] question regarding rules for disposal of slides cassettes
Not sure why we don't already have a policy for this, but my histotechnologist wanted me to find out how to properly dispose of stained/coverslipped slides and cassetes with FFPE tissue. Our lab only processes animal tissue (no TSE material). I tried searching the internet, but didn't find anything. Thanks in advance for any advice! -J Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fwd: [Histonet] Intellipath Instrument
Sorry.forgot to reply to the list...see my response below -Original Message- From: godsgal...@aol.com To: micropathl...@yahoo.com Sent: Fri, 20 Mar 2009 4:41 pm Subject: Re: [Histonet] Intellipath Instrument This company has always provided the best service and we are getting 10 instruments, if that tells you anything Roxanne -Original Message- From: Sheila Haas micropathl...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Fri, 20 Mar 2009 10:28 am Subject: [Histonet] Intellipath Instrument A colleague of mine is looking into purchasing an immuno stainer. Biocare has offered a good price on the?Intellipath instrument, antibodies and reagents. While we know the antibodies are reagents are excellent, we know?nothing about?their instrumentation. Does anyone have any first hand?experience with?the Intellipath instrument, service from?Biocare, etc...? Any information would be helpful. Thank you in advance. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet See Your Credit Report in Seconds! Easy to Read and Viewable Online. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Current books for Histotechnology
Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: dako autostainers
Jennifer, I have an older model Dako Autostainer that still works well. Dako does still service it, too. It had a printer available, but we chose not to use it. Our volume is small, ~2400 slides yr. The size/type stainer you choose should be based on the number of immuno's that you do a year. NOT on what Dako wants to push on you, equipment or antibody systems. And I do mean PUSH. They tried for a year to Push their new FLEX system on us. It becomes a closed system and much more expensive. Do not allow them to do it to you. I was on the verge of going to a competitor. If you push back, they will back off. I still use Dako for all my IHC, but, again, I may start looking closer at the competition because the corporation is not giving the small lab the breaks that we need to keep up with the big hospitals. It's sad that the Dako leadership is not paying attention to their customers. Dako, Are you listening? I'm not sure how much this helps you, but I feel better by letting someone know that they are/were a good company, but is under bad leadership at the moment. Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax -- Message: 5 Date: Thu, 19 Mar 2009 08:45:23 -0700 From: Jennifer Campbell jcampb...@vdxpathology.com Subject: [Histonet] dako autostainers To: histonet@lists.utsouthwestern.edu Message-ID: 5658cbdb9eae6545abe50d2563d81bf81df...@vdxserver01.vdxpathology.local Content-Type: text/plain;charset=us-ascii We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the older models anymore b/c they are worried that the software would be out of date. I looked into this on Dako.com and found that there is the older Dado Autostainer. After reading through its description it looks like it just lacks a few of the bells and whistles (like a slide labeler for example) that the Autostainer Plus has but, otherwise looks like it gets the job done just the same. Does anyone have any feedback on either one of these machines and whether or not you think I should even bother looking into the older model? Thank you so much. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processing v-e-r-y tiny samples
Well, you could always hand process them in test tubes. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Thu, 19 Mar 2009 16:39:14 -0400 From: bryan.wat...@parkview.com To: histonet@lists.utsouthwestern.edu; algra...@u.arizona.edu Subject: Re: [Histonet] processing v-e-r-y tiny samples CC: I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra...@u.arizona.edu 3/19/2009 11:51 AM Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Internet Explorer 8 – Now Available. Faster, safer, easier. http://clk.atdmt.com/MRT/go/141323790/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet