RE: [Histonet] RE: Histonet Digest, Vol 65, Issue 7
As a recovering Cytologist in the UK I think we have to screen about 7,000 slides per annum, but a 'Checker' less than that and a Medic even less. But the rules may have changed as I've been out of Cytology for a number of years. I know that Jade will have increased the number of women wanting smears (can we can LBC smears?). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: 03 April 2009 13:42 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 65, Issue 7 Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 Hi Alyssa - Get ready...here is the dirt CAP follows CLIA-88, and both are quite clear on limits for Cytotech screening. They are no more than 100 slides in 24 hours and include gyn and non-gyn including new routine slides, 10% rescreen slides, and 5 year look back negative slides the maximum workload of 100 slides can be completed in no less than an 8-hour workday. This workload can also be expressed as slides per hour and is 12.5 slides/hour. These total limits apply regardless of the number of laboratories in which an individual works on a given day. Wait! it gets better For primary screening of gyn liquid based preps (read as Thin Prep) each slide must be counted as a single slide for the purpose of workload recording. For primary screening of non-gyn liquid based preps (read as Thin Prep) each slide may be counted as one-half slide for the purpose of workload recording, provided that the cells are dispersed over one-half or less of the total available slide area. Wait! there's still more... Workload calculations may vary with the use of automated screening instruments. Laboratories should follow manufacturers instructions for workload calculations and must assure that CLIAA-88 requirements are fulfilled. and just when you thought it was safe The laboratory director must establish the maximum workload (based on the capability/documented performance evaluation) for each individual examining slides and the limit must be reassessed at least every 6 months. The regulations go on to explain how the reassessment is to be done...blah, blah, blah Though my background and education are in Histology, I've served as technical supervisor over cytology departments for over 14 years, and the above listed %#@! is why Cytology can drive you up the wall. Our cytotech's, when we had them, screened no more than 80 slides/day, when all they did was screen. No pulling slides, no running up to do an FNAjust sitting non-stop and screening slides. UGH! Sorry Histo folk - but some of you are probably in the same position as I am, so please feel free to chime in. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problems with mouse brains
Hello Kemlo, I am Portuguese-Spanish so we use the word tampón that I think I miss translated to tamponated..really do not know if the word exists! but apparently it does not! :) I want to mean when you add salt to the formol dilution in destilated water in order to prepare your 10% buffered formalin... a believe the word is buffered So sorry..for my wrong translationI was in a hurry...what I want to say is THAT YOUR FORMALIN IN NOT WELL BUFFERED :) thank you! and Yes, you should be carefull your brain is well fixated in formalin at least for 24 hour. 2009/4/5 kemlo ke...@f2s.com Agree, poor formalin fixation will allow nuclear bubbling which some authorities put down to the effects of heat on the sub optimally stabilised proteins. What a marvellous phrase your formol is not well tamponated. Wish I had thought of that.. I will of course steal it if you allow such plagiarism? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ana Resendes Sent: 04 April 2009 15:16 To: Randolph-Habecker, Julie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Problems with mouse brains have very light HE staining: first you should check if either your eosin or haematoxilin are over used nuclear bubbling: can be a lot of things but can be inclusion. Brain sections are big so make at least a 24 h inclusion process. and extensive tissue cracking: could be over heating or bad inclusion. You have to check if reagents in inclusors are ok or over passed, I would change all reagents. I also have noticed some formalin pigment: your formol is not well tamponated 2009/4/3 Randolph-Habecker, Julie jhabe...@fhcrc.org Folks, I need some input on a problem we're seeing with some mouse brain samples. The samples are from new born mice P0 to P14 which have been fixed in 10% NBF (Fisher brand and well within expiration date) for 72 hours. They are then transferred to 70% etoh and processed on an 8 hour process. After the tissue is embedded, we are taking sagital sections, drying them overnight, and then baking overnight at 60C. They are then stained with HE. This has been working great but now all of a sudden we have very light HE staining, nuclear bubbling, and extensive tissue cracking. I also have noticed some formalin pigment. My first thought is that there was difference in formalin or time in formalin but that is not the case. I also wondered if the tissue might be exposed to excessive heat. We checked all of the temperatures in our processor, embedding center, and water baths - all were within tolerance. Any ideas what might cause all three artifacts? Could it be from inadequate paraffin infiltration? Any help would be greatly appreciated! Thanks, Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Ana Resendes DVM, MSc, PhD Veterinary pathologist Centro de Investigação de Recursos Naturais (CIRN) Secção de Anatomia e Taxonomia Zoológicas, Departamento de Biologia, Universidade dos Açores. Rua da Mãe de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (Açores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Ana Resendes DVM, MSc, PhD Veterinary Pathologist Centro de Investigação de Recursos Naturais (CIRN) Secção de Anatomia e Taxonomia Zoológicas, Departamento de Biologia, Universidade dos Açores. Rua da Mãe de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (Açores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Stainers
Hello- I am looking for any comments (positive or negative) on IHC stainers. Any info on DAKO, Ventana, Leica, and Biocare would be great. Service, quality, ease of use, etc. I have very little knowledge of any of these as I have not done IHC for many years. Thanks for all your input!! -Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC Stainers
Very recently (just a few days ago) this topic was discussed in HistoNet. You would be better served if you check the Archives for answers. René J. --- On Sun, 4/5/09, kristen arvidson arvidsonkris...@yahoo.com wrote: From: kristen arvidson arvidsonkris...@yahoo.com Subject: [Histonet] IHC Stainers To: histonet histonet@lists.utsouthwestern.edu Date: Sunday, April 5, 2009, 11:14 AM Hello- I am looking for any comments (positive or negative) on IHC stainers. Any info on DAKO, Ventana, Leica, and Biocare would be great. Service, quality, ease of use, etc. I have very little knowledge of any of these as I have not done IHC for many years. Thanks for all your input!! -Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] silanising slides
hi everyone, can any one tell me how many slides can be processed with 10ml of 3-aminopropyltriethoxysilane. i have a 100ml solution from Sigma. Regards, Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_an...@hotmail.com Phone: +91-9940475801 _ Windows Live Messenger. Multitasking at its finest. http://www.microsoft.com/india/windows/windowslive/messenger.aspx___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] nice one Kemlo
The well known German poet Rainer Maria Rilke (1875-1926) was originally named René, and Germanized the name to Rainer when he was a young man. His mother supposedly gave him the name in memory of a recently deceased older sister, but the story that she dressed the future poet as a girl may be untrue. Bernhard Blume, my major professor in 1959 and an elderly man then (I majored in German in college, and wrote my senior thesis on Rilke, back before he got famous in English) told me that his baby pictures showed him dressed quite like the young Rilke. Bob Richmond Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Texas Society for Histotechnology 2009 Symposium/Convention-Registration is Open
Dear Histonetters: It's not too late to sign up for the Texas Society for Histotechnology meeting. Pre registration for the meeting is still available and deadline is MAY 25, 2009. Attached is the 2009 TSH convention preview in Austin,Texas May 29-31, 2009 (not Memorial Day weekend). The Convention will be held at the Hyatt Regency, 208 Barton Springs Road, Austin,Texas. Please join us for a great Texas Style meeting. All workshops will be contact hour approved by NSH. For more information or a e-mail copy of the program contact: veron...@baylorhealth.edu or kdywer3...@aol.com or www.txsh.org Friday, May 29, 2009 WS#1- Skip Brown-Basics Dynamics of Fixation and Processing WS#2 – Damien Matusiak- Digital Evolution: Taking the Pathologists from the Basement to the Bedside Saturday, May 30, 2009 WS#3 - Jan Gardner - Competency Assessment Program WS#4 – Jennifer Hofecker – Understanding CJD WS#5 – Nora Lacey – 2009 Latest Antibody Information and IHC Applications WS#6 – David Tate – Serial Killers WS#7 – Skip Brown – The Wax Museum: Understanding Paraffins WS #8 – Bill DeSalvo/Lamar Jones – From Concept to Reality-Implementing Continuous Process Flow in the Histology Lab WS#9 – Donna Willis – Rapid Tissue Processing, A New Era 2009 WS#10 – Jim Chalmers – Pretreatments – An Important Toll in Immunohistochemistry WS #11 – Terri Crook, MD – Cytology for th e Histotechnologist WS#12 – Charlotte Sanders – Safety Can Be Fun!! Sunday, May 31, 2009 WS #13 – Jennifer Hofecker – The Wonderful World of Neuropathology WS #14 – Bonnie Whitaker/Pam Marcum – Two Crazy Cat Ladies Give Their Opinions of Life, Liberty and Lab Management WS#15 – Debbie Siena/Brent Hart – Immunohistochemistry: Validation vs. Optimization WS#16 – Debra Cohen/Sharon Tandy – Paraffin-Embedded Tissue Fluorescence In Situ Hybridization (PET FISH) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] nice one Kemlo
My grandfather from Slovakia was Jan. My name is Jackie, I have five sisters, two are Jeri and Stevie. My parents had a son after they had five daughters and named him Ernie.Sometimes people just run out of good names for their kids.I happen to like Rene'. It beats Apple or Moon Unit Zappa. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Burned Tissue
I would suggest checking the fixation. We had similar problems if the tissue was not completely fixed. Are the tissues free to move around in the cassettes (ie have free unhindered access to the fixative)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of thisis...@aol.com Sent: Friday, 3 April 2009 5:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Burned Tissue Recently we are experiencing random blocks of tissue (approx. 3 blocks out of 400/2x) that appear to be burned.? The tissue is prostate and the staining looks dark.? Upon recutting, sometimes it is better, sometimes it is not, which negates my ability to rule out the stainer completely. We process on a 2 hour schedule using the Peloris and all tissue is processed together.? I am not understanding how, out of a 12 part prostate, only one block stains so dark so randomly.? When recut, some blocks are better, some still have the dark staining. Does anyone have any suggestions? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Entamoeba Trophozoites
It's amazing how such a simple observation can bring out the worst (and most subjective rants) in some people, eh? How many personal insults and names did you just hurl at me here, Sonny? the most ironic part is that people like you jump down my throat for my comments but don't seem to be bright enough to restrain themselves from making the exact same sort of comments. And YOU're the one calling ME a half wit! From: kemlo ke...@f2s.com To: Bernie Taupin bernietau...@ymail.com; histonet@lists.utsouthwestern.edu Sent: Saturday, April 4, 2009 9:15:02 AM Subject: RE: [Histonet] Entamoeba Trophozoites Is there a problem with that sonny? Maybe you ought to get the gender right before you attack someone who tries very hard to help people. Ignorant people, such as you, usually attack those brighter than themselves. The Middle Ages beckons you, sonny! Kemlo Rogerson Pathology Manager used to dealing with half wits (comes with the territory). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 04 April 2009 06:21 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Entamoeba Trophozoites Anyone else think this Rene fella comes across as a real know-it-all? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; Lynette Pavelich lpave...@hurleymc.com Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. René J. --- On Fri, 4/3/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nice one Kemlo
1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; alan taylor aj.tay...@blueyonder.co.uk Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am a legitimate April Fool and I intend to keep doing what I am doing for as long as I can. René J. --- On Sat, 4/4/09, alan taylor aj.tay...@blueyonder.co.uk wrote: From: alan taylor aj.tay...@blueyonder.co.uk Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Entamoeba Trophozoites
I am a HE and not a SHE Oh, HAHAHA! Nice one, Kemlo! Disregard my last noes re: Gender and maybe, I dunno, CHECK YOUR OWN FACTS BEFORE CHEWING SOMEONE OUT FOR THEIRS? Everyone on this list is so quick to jump down someone else's throat for telling it like it is, completely ignorant of their own breaches in tact, their own ignorances, their own off-topicness. Everyone loves pointing the finger, yet no one can tolerate it when its actually them at fault. You guys crack me up with your logic sometimes. I fear for the world that the safety of so many imporant biopsies are trusted to so many of yours' easily-agitated, ignorant hands! From: kemlo ke...@f2s.com To: Bernie Taupin bernietau...@ymail.com; histonet@lists.utsouthwestern.edu Sent: Saturday, April 4, 2009 9:15:02 AM Subject: RE: [Histonet] Entamoeba Trophozoites Is there a problem with that sonny? Maybe you ought to get the gender right before you attack someone who tries very hard to help people. Ignorant people, such as you, usually attack those brighter than themselves. The Middle Ages beckons you, sonny! Kemlo Rogerson Pathology Manager used to dealing with half wits (comes with the territory). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 04 April 2009 06:21 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Entamoeba Trophozoites Anyone else think this Rene fella comes across as a real know-it-all? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; Lynette Pavelich lpave...@hurleymc.com Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. René J. --- On Fri, 4/3/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Entamoeba Trophozoites
All critique and contention with Bernie's opinions aside, I have recently grown flabbergasted by the surprising unprofessionalism of some of HistoNet's responders, an the complete, utter and profound lack of tact expressed in some of the Reply-to-All messages. I mean, honestly. We have hotheaded individuals like Kemlo mocking and correcting people about things that they were correct about to begin with, we have individuals getting laughably snagged by the snares of irresistable, pointless argumentativism and we simply have indivudals proving, over and over again, that the worsat of human nature tends to come out when replying to internet Trolls. So do us all a favor, Bernie, Kemlo, Rene and everyone else here and give it a freakign rest already. If you're engaging trolls in arguments or seemingly-witty (yet in hindsight, ignorantly and laughably incorrect) commentary, then you're as much of a problem as the Troll himself, and I should hope to see such inane individuals (as well as all the collective eye-rolling and grimacing that they cause) exilded from this list!!! ~L. --- On Sat, 4/4/09, kemlo ke...@f2s.com wrote: From: kemlo ke...@f2s.com Subject: RE: [Histonet] Entamoeba Trophozoites To: 'Bernie Taupin' bernietau...@ymail.com, histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 9:15 AM Is there a problem with that sonny? Maybe you ought to get the gender right before you attack someone who tries very hard to help people. Ignorant people, such as you, usually attack those brighter than themselves. The Middle Ages beckons you, sonny! Kemlo Rogerson Pathology Manager used to dealing with half wits (comes with the territory). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 04 April 2009 06:21 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Entamoeba Trophozoites Anyone else think this Rene fella comes across as a real know-it-all? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; Lynette Pavelich lpave...@hurleymc.com Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. René J. --- On Fri, 4/3/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Expert Fees/Salaries
Did anyone else happen to see that online clip from Dateline regarding Histology Expert Fees/Salaries? I am curious to know exactly what they mean by expert...? See clip here: http://tinyurl.com/2g9mqh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] René as a name.
For all those not cognizant of it, including the eminently intellectual Mr. Taupin, the name René is a male French name. The tip off is the acute accented é at the end. The female name is Renée. René's name is not androgynous at all, it is quite clearly male, and anyone who has studied French should be aware of it. Mr. Taupin is clearly trolling, so could we please have him removed from this group? Bryan Llewellyn - Original Message - From: Bernie Taupin bernietau...@ymail.com To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor aj.tay...@blueyonder.co.uk Sent: Sunday, April 05, 2009 9:27 PM Subject: Re: [Histonet] Nice one Kemlo 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; alan taylor aj.tay...@blueyonder.co.uk Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am a legitimate April Fool and I intend to keep doing what I am doing for as long as I can. René J. --- On Sat, 4/4/09, alan taylor aj.tay...@blueyonder.co.uk wrote: From: alan taylor aj.tay...@blueyonder.co.uk Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
