[Histonet] RE: TMAs for Cell Pellets - Histonet Digest, Vol 65, Issue 22

[Thom Jensen]

If you are making a cell blocks with your cell pellets then punching the cell 
block and inserting it into a TMA then is ideal.  The cell pellets we have made 
were created like a cell block.  The cells are spon down, the fluid is poured 
off and the cell button is mixed with a augar type substance (venders would 
have info on the augar type material used for making cell blocks).  Once the 
solution solidifies it is placed in a paper or mesh bage and placed into a 
cassette for processing.  Once processed the cells are imbedded into a paraffin 
block as you would tissue.  A fresh H&E is cut off the block and areas of 
interest are marked on the slide to show the best spot to punch for the TMA. 
You will also find that the TMA instrument can pay for itself sometimes after 
one protocal.  By this I mean you will save on antibody cost and have 
consistency in your staining since all samples are on one slide instead of many 
slides.  I have constructed regular paraffin TMAs, cell arrays, sponge arrays 
(cells growing in a sponge type material processed and embedded in paraffin) 
and frozen TMAs.  It just takes a little expermenting, some patience and 
pratice making TMAs.

cheers,
Thom


> Date: Tue, 14 Apr 2009 10:20:40 -0400
> From: la...@email.med.yale.edu
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 22
> 
> Give me a shout in regards to cell pellets & TMA's.
> There are several methods and some are less expensive then others.
> Have a good one.
> Lori
> 
> 
> 
> Beecher is usually difficult to deal 
> with.histonet-requ...@lists.utsouthwestern.edu wrote:
> > Send Histonet mailing list submissions to
> > histonet@lists.utsouthwestern.edu
> >
> > To subscribe or unsubscribe via the World Wide Web, visit
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > or, via email, send a message with subject or body 'help' to
> > histonet-requ...@lists.utsouthwestern.edu
> >
> > You can reach the person managing the list at
> > histonet-ow...@lists.utsouthwestern.edu
> >
> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of Histonet digest..."
> >
> >
> > Today's Topics:
> >
> >1. Re: TMA for cell pellets? (Bernie Taupin)
> >2. To all spam gourmets (V. Neubert)
> >3. RE: I recommend NOT opening that Attachment: (Hugh Luk)
> >4. RE: TMA for cell pellets? (Hartson, Louise)
> >5. Re: Formula 83 users? (Rene J Buesa)
> >6. Re: Negative IHC controls (Rene J Buesa)
> >7. RE: Formula 83 users? (Blazek, Linda)
> >8. Re: Spyware from an Attachment: (Geoff McAuliffe)
> >9. Formula 83-Thank you! (Jacqueline Farnsworth)
> >   10. HISTOS 5 processor (FU,DONGTAO)
> >   11. Position Open In Mass (Alyssa Peterson)
> >   12. Re: HISTOS 5 processor (Robert Schoonhoven)
> >   13. Travelling Histotechs (Robert Schoonhoven)
> >   14. Regional Sales Manager position- West Coast
> >   (kris.caldw...@leica-microsystems.com)
> >   15. New lab, need some info (Putnam, Jodi)
> >
> >
> > --
> >
> > Message: 1
> > Date: Wed, 8 Apr 2009 22:50:26 -0700 (PDT)
> > From: Bernie Taupin 
> > Subject: Re: [Histonet] TMA for cell pellets?
> > To: Thom Jensen ,
> > louise_hart...@urmc.rochester.edu,  
> > histonet@lists.utsouthwestern.edu
> > Message-ID: <338565.20208...@web43513.mail.sp1.yahoo.com>
> > Content-Type: text/plain; charset=iso-8859-1
> >
> > Recently, someone mentioned putting a library of protocols online, perhaps 
> > in the HistoNet archives. I'd like to second that emotion if its possible 
> > to make happen... What a great resource that would be!
> >
> >
> >
> >
> > 
> > From: Thom Jensen 
> > To: louise_hart...@urmc.rochester.edu; histonet@lists.utsouthwestern.edu
> > Sent: Thursday, April 9, 2009 1:27:18 AM
> > Subject: [Histonet] TMA for cell pellets?
> >
> >
> >
> > Have you tried to embed your cell pellets into a tissue microarray?  Two 
> > companies I would recommend:
> > www.arraymold.com
> > www.beecherinstruments.com
> >
> > Two of the best TMA products on the market.
> >
> >
> > cheers,
> >
> >   
> >> Date: Thu, 2 Apr 2009 12:05:39 -0400
> >> From: louise_hart...@urmc.rochester.edu
> >> To: histonet@lists.utsouthwestern.edu
> >> Subject: [Histonet] Embedding cell pellets
> >>
> >>
> >> I am looking for a protocol for embedding cell pellets in paraffin.
> >> Thanks,
> >> Louise
> >>
> >> Louise Hartson, BA
> >> Senior Technical Associate
> >> University of Rochester
> >> louise_hart...@urmc.rochester.edu
> >> ___
> >> Histonet mailing list
> >> Histonet@lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >> 
> >
> > _
> > Hotmail® is up to 70% faster. Now good news travels really fast.
> > http://windowslive.com

RE: [Histonet] container for undiluted bleach

[Tony Henwood]

Why do need to store or use undiluted bleach?
I would dilute it to a working solution and use that.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Thursday, 16 April 2009 6:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] container for undiluted bleach


Hello again

We keep a small squirt bottle of undiluted bleach in our hood but it's
constantly eating through the plastic. Any ideas on other ways to store
about 150 ml in a squirt bottle or what type of plastic is bleach-proof?
The point here is that we want to store a small amount in the hood and
possibly be able to squirt instead of pour it.

Emily
prometheus, thief of light, giver of light, bound by the gods, must have
been a book. -mark danielewski, house of leaves
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Re: [Histonet] prostate biopsy kits (LONG message sorry)

[Dana Spears]

Jodi,
I order my prostate kits from PATH-TEC.  I met them at the NSH and they
are such an awesome company!  They customized the whole kit AND reqs for
me.  They even fold the reqs and pack them in the box!  I can't say
enough about this company-i was just amazed at the service and price. 
They really go above and beyond and can even set it up so your clients
go online and just "order" your kits and they get billed to you.  

Good luck! 
Dana Spears, HTL 
Laboratory Manager  Methodist Medical CTR  309.672.4930 (office)
309.255.7214 (cell) 309.279.3768 (fax) 
 
Sent from my Verizon Wireless BlackBerry  
-Original Message- 
From: "Putnam, Jodi"  
To:   
 
Sent: 4/15/2009 5:48:19 PM 
Subject: [Histonet] prostate biopsy kits (LONG message sorry) 
 
Hi again. 
I am in need of information and pricing on prostate biopsy kits. We have
just started taking on prostate biopsies. 
The kit that I am receiving holds 12 "20 ml" specimen bottles and they
are made by Bostwick Labs. The urologists  
were sending them out to be processed but now that we have a pathology
lab, it is in everyone's best interest to keep 
them in house. I love that we can give a quicker turnaround time for the
doctors that are used to sending them out.  
My problem is this, once I use up all of the kits that the urologists
have already bought, I will need my own. I need to keep the same 
order that they are used to, all of the 12 sites are preprinted below
the hole that holds the specimen bottle in the box. (Maybe the 
list is a standard template used by all doctors now for prostate
biopsies, I don't know.) 
I was going to try to come up with something myself to save money,
homemade. I don't have time to shop around for the best price, 
but is it best for me to buy the kits? I just did a quick glance online
and saw some kits (12 specimen) for 7.50/each. Is that good or 
can I do better? OR do any of you have an idea for the homemade version?
I just need to make sure that my sites are exactly 
in the order that the doctors are used to seeing and 3 rows, left to
right, top to bottom. I was going to reuse the boxes if they were 
 not visibly contaminated and just put in 20 ml prefilled formalin
bottles, but I am not sure that is a good thing. I worry about  
hygiene, cross contamination etc. Any help or words of wisdom would be
great. If I had the time I would shop around but I have 
no spare time right now. I thought about getting something like the old
plastic test tube racks (only with bigger holes) and putting 
solvent resistant labels on them and sanitizing them prior to returning
to the urology dept. with new prefilled formalin bottles. 
Is that feasible?  
Any sales reps that are on the histonet, please email me any quotes on
the kits. I am having a hard time fielding all the phone calls 
right now. 
Thanks so much.  
Jodi 
  
Jodi Putnam (HT,ASCP) 
Graves Gilbert Clinic 
Pathology Department 
201 Park Street 
Bowling Green, KY 42102 
(270) 393-2728 (voicemail) 
(270) 393-2795  
Fax : (270) 393-2736 
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
_ _ 
 
This email and any files transmitted with it are confidential and 
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Any views expressed in this message are those of the individual 
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Thank you for your assistance with this matter.

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[Histonet] prostate biopsy kits (LONG message sorry)

[Putnam, Jodi]

Hi again.
I am in need of information and pricing on prostate biopsy kits. We have just 
started taking on prostate biopsies.
The kit that I am receiving holds 12 "20 ml" specimen bottles and they are made 
by Bostwick Labs. The urologists 
were sending them out to be processed but now that we have a pathology lab, it 
is in everyone's best interest to keep
them in house. I love that we can give a quicker turnaround time for the 
doctors that are used to sending them out. 
My problem is this, once I use up all of the kits that the urologists have 
already bought, I will need my own. I need to keep the same
order that they are used to, all of the 12 sites are preprinted below the hole 
that holds the specimen bottle in the box. (Maybe the
list is a standard template used by all doctors now for prostate biopsies, I 
don't know.)
I was going to try to come up with something myself to save money, homemade. I 
don't have time to shop around for the best price,
but is it best for me to buy the kits? I just did a quick glance online and saw 
some kits (12 specimen) for 7.50/each. Is that good or
can I do better? OR do any of you have an idea for the homemade version? I just 
need to make sure that my sites are exactly
in the order that the doctors are used to seeing and 3 rows, left to right, top 
to bottom. I was going to reuse the boxes if they were
 not visibly contaminated and just put in 20 ml prefilled formalin bottles, but 
I am not sure that is a good thing. I worry about 
hygiene, cross contamination etc. Any help or words of wisdom would be great. 
If I had the time I would shop around but I have
no spare time right now. I thought about getting something like the old plastic 
test tube racks (only with bigger holes) and putting
solvent resistant labels on them and sanitizing them prior to returning to the 
urology dept. with new prefilled formalin bottles.
Is that feasible? 
Any sales reps that are on the histonet, please email me any quotes on the 
kits. I am having a hard time fielding all the phone calls
right now.
Thanks so much. 
Jodi
 
Jodi Putnam (HT,ASCP)
Graves Gilbert Clinic
Pathology Department
201 Park Street
Bowling Green, KY 42102
(270) 393-2728 (voicemail)
(270) 393-2795 
Fax : (270) 393-2736
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom
they are addressed.
If you have received this email in error please notify the
originator of the message. This footer also confirms that this
email message has been scanned for the presence of computer viruses.

Any views expressed in this message are those of the individual
sender, except where the sender specifies and with authority,
states them to be the views of Graves-Gilbert Clinic.

Scanning of this message and addition of this footer is performed
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virus detection software.



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[Histonet] collagen III antibody

[Amy Lee]

Hello,
I am looking for a collagen III antibody that works on FFPE mouse tissue. Could 
anybody recommand one please?
 
Thanks,
Amy



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[Histonet] container for undiluted bleach

[Emily Sours]

Hello again

We keep a small squirt bottle of undiluted bleach in our hood but it's
constantly eating through the plastic.
Any ideas on other ways to store about 150 ml in a squirt bottle or what
type of plastic is bleach-proof?
The point here is that we want to store a small amount in the hood and
possibly be able to squirt instead of pour it.

Emily
prometheus, thief of light, giver of light, bound by the gods, must have
been a book.
-mark danielewski, house of leaves
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Re: [Histonet] revalidation form for renewal of QIHC

[stephanie . d . rivera]

Hello,

Go to ascp.org, click certification, icon get qualified(should be on the 
left), go down to Step # 7 Revalidate after 5 years click QIHC.



Stephanie D. Rivera
Safety Assessment Department
GlaxoSmithKline
709 Swedeland RD
King of Prussia, PA 19406
phone: 610-270-7340
fax: 610-270-7202



sris...@mail.holyname.org 
Sent by: histonet-boun...@lists.utsouthwestern.edu
15-Apr-2009 14:35
 
To
histonet-boun...@lists.utsouthwestern.edu, 
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] revalidation form for renewal of QIHC







Hello All

Does anyone know what website to use to print out the QIHC revalidation
form inorder to renew the certification?

Thanks

Nirmala Srishan



















__

You can view the MSDS for CBG Biotech's "Formula 83" xylene substitute at

www.cbgbiotech.com/msds/MSDS-Formula83071007.pdf

It's described as a "naphthenic hydrocarbon blend".

I'd note the low flash point (7 C, 45 F), considerably lower than
xylene's. Other aliphatic or naphthenic (cycloalkane) mixtures offered
as clearing agents have considerably higher flash points.

Remember that if you want to recover it by distillation, you must make
sure that your still has a distillation routine for it, and you cannot
change clearing agents without changing the distillation routine, nor
can you mix the solvents and recover them by distillation.

If your lab manager shares cost information with you (in my experience
most do not) then cost will certainly be a consideration, if it's
indeed cheaper than xylene.

According to the MSDS, disposal is done like any other aliphatic or
naphthenic clearing agent.

Bob Richmond
Samurai Pathologist
Knoxville TN

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Holy Name Hospital is the recipient of:



  2005, 2006, 2007 & 2008 Distinguished Hospital Awards for Clinical
  Excellence, HealthGrades

  2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary
  Care, HealthGrades

  2006 & 2007 Awards for Emergency, Outpatient and Inpatient Service
  Excellence, J.D. Power

  Primary Stroke Center Designation, The Joint Commission

  Chest Pain Center Accreditation , Society of Chest Pain Centers

  2006, 2007 & 2008 Best Places to Work in New Jersey, NJBIZ


The information contained in this message is privileged and CONFIDENTIAL
and is intended only for the use of the addressee above.  If you are not
the intended recipient, you are hereby notified that any disclosure,
copying, distribution or taking of any action in reliance on the content 
of
this message is strictly prohibited.


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[Histonet] Amyloid A- IHC stain

[Cazares, Ruth]

Hello fellow Histonetters,

I was wondering if anyone is running Biocare's Amyloid A or Amyloid P 
antibodies on Ventana's Benchmark. If so, are you using the concentrated or 
predilute antibody?

Also, are you running these alone or with a Congo Red stain (as in a panel)? 
Any information is greatly appreciated.

Thank you,

Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

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[Histonet] Microtome and Human Tissue Blocks

[anitaibsc]

Hello Histonetters,


We are looking for:

1) A used Microm Microtome, in good condition, either automated rotary or 
retracted rotary or other kind.

2) Human tissue blocks, Formalin-Fixed-Paraffin-Embedded (FFPE), several 
cancers and normal tissues.

Thank you.

Bader Sidikki, PhD
R&D Director
Email: bade...@gmail.com 
Web site: www.ImmunoBioScience.com 

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[Histonet] revalidation form for renewal of QIHC

[srishan]

Hello All

Does anyone know what website to use to print out the QIHC revalidation
form inorder to renew the certification?

Thanks

Nirmala Srishan



















__

You can view the MSDS for CBG Biotech's "Formula 83" xylene substitute at

www.cbgbiotech.com/msds/MSDS-Formula83071007.pdf

It's described as a "naphthenic hydrocarbon blend".

I'd note the low flash point (7 C, 45 F), considerably lower than
xylene's. Other aliphatic or naphthenic (cycloalkane) mixtures offered
as clearing agents have considerably higher flash points.

Remember that if you want to recover it by distillation, you must make
sure that your still has a distillation routine for it, and you cannot
change clearing agents without changing the distillation routine, nor
can you mix the solvents and recover them by distillation.

If your lab manager shares cost information with you (in my experience
most do not) then cost will certainly be a consideration, if it's
indeed cheaper than xylene.

According to the MSDS, disposal is done like any other aliphatic or
naphthenic clearing agent.

Bob Richmond
Samurai Pathologist
Knoxville TN

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Holy Name Hospital is the recipient of:



  2005, 2006, 2007 & 2008 Distinguished Hospital Awards for Clinical
  Excellence, HealthGrades

  2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary
  Care, HealthGrades

  2006 & 2007 Awards for Emergency, Outpatient and Inpatient Service
  Excellence, J.D. Power

  Primary Stroke Center Designation, The Joint Commission

  Chest Pain Center Accreditation , Society of Chest Pain Centers

  2006, 2007 & 2008 Best Places to Work in New Jersey, NJBIZ


The information contained in this message is privileged and CONFIDENTIAL
and is intended only for the use of the addressee above.  If you are not
the intended recipient, you are hereby notified that any disclosure,
copying, distribution or taking of any action in reliance on the content of
this message is strictly prohibited.


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[Histonet] mouse tibia 200um sections-low speed saw?

[Jamie E Erickson]

Hi Histonetters,
   Can any one help I am trying to repeat 
what I see in this paper and it said it cut cortical mouse bone  tibia and 
I quote  "For the cortical bone, a pair of 100–200 um-thick
transverse sections were cut in the middle of the tibia with a low-speed 
diamond blade saw."

I tried to cut this thin but I can't not get that thin... I am using a 
buehler isomet low speed saw with a 4" 15HC  wafering blade. My micrometer 
reads every complete turn from zero to zero = 625um or 25 MIL and that is 
about as thin as I can cut mouse tibia. Please help am I missing 
something, what is the trick...??

Thanks

Jamie


___
Jamie Erickson
Sr. Research Associate II M.S. HTL (ASCP)
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erick...@abbott.com


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[Histonet] Missouri Society for Histotechnology 2009 Symposium

[Jean-Philippe REY]

The Missouri Society for Histotechnology will have its annual symposium hold at 
the beautiful Lake of the Ozarks in a remarkable facility, Resort at Port 
Arrowhead from Thursday May 28th to Saturday May 30th, 2009. 
 
To obtain more information:


Check our website at: 
http://www.missouri-histo.org/index.cfm?p=4&pid=4274&spid=4295

Or our video at: http://www.youtube.com/watch?v=6TgQAJ7J6UM
 
We are looking  forward meeting you there!
 
 
Jean-Philippe REY
MHT Vice President
4441 Gillham Road
Kansas City, MO -64110
jp10...@hotmail.com
www.missouri-histo.org
Tel.:(816) 213-2558
Tel.:(913) 945-6763
Fax.: (913) 660-0637___
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[Histonet] Missouri Society for Histotechnology Annual Symposium

[JP Rey]

The Missouri Society for Histotechnology will have its annual symposium hold
at beautiful Lake of the Ozarks in a remarkable facility, Resort
  at Port Arrowhead from
Thursday May 28th to Saturday May 30th, 2009. For more information check our
website at: http://www.missouri-histo.org/index.cfm?p=4

&pid=4274&spid=4295

Or our video at: http://www.youtube.com/watch?v=6TgQAJ7J6UM

We are looking  forward meeting you there!

 

 

Jean-Philippe REY

MHT Vice President

4441 Gillham Road

Kansas City, MO -64110

jp10...@hotmail.com

www.missouri-histo.org  

Tel.:(816) 213-2558

Tel.:(913) 945-6763

Fax.: (913) 660-0637

 

 

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[Histonet] Marilyn A Weiss is out of the office as of 11:30 a.m. 4/15/2009

[Marilyn . A . Weiss]

I will be out of the office starting  04/15/2009 and will not return until
04/20/2009.

I will respond to your message when I return.In my absence please ask for
Mary Campbell at 619-528-6801  if this is urgent.


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RE: [Histonet] 5 Reasons - The list

[Blazek, Linda]

Tom,
You must work at my facility!  Those are all of the reasons here.  (And that 
isn't said with tongue in cheek at all)

Linda
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom
Sent: Wednesday, April 15, 2009 12:52 PM
To: 'Cindy DuBois'; Histonet
Subject: RE: [Histonet] 5 Reasons - The list

Hi Cindy, Maybe the next 5 would be ; The pay is great, The bosses are very 
caring, The hours are flexible, The equipment is topnotch, The coworkers are 
brilliant. Have a great day, Tom Truscott

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois
Sent: Wednesday, April 15, 2009 5:53 AM
To: Histonet
Subject: [Histonet] 5 Reasons - The list

Thanks to so many of you I have come up with the following list:

1.  You get to dig into peoples brains like no psychologist can.
2.  Job security - no one else wants to work in the "human parts department".
3.  It is never boring - "How did the patient get THAT in THERE?"
4.  You get to do arts and crafts (Wax & Stains).
5.  Most important - Providing quality care to patients.

Cind Dubois
Integrated Pathology
Stockton, CA

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RE: [Histonet] 5 Reasons - The list

[Truscott, Tom]

Hi Cindy, Maybe the next 5 would be ; The pay is great, The bosses are very 
caring, The hours are flexible, The equipment is topnotch, The coworkers are 
brilliant. Have a great day, Tom Truscott

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois
Sent: Wednesday, April 15, 2009 5:53 AM
To: Histonet
Subject: [Histonet] 5 Reasons - The list

Thanks to so many of you I have come up with the following list:

1.  You get to dig into peoples brains like no psychologist can.
2.  Job security - no one else wants to work in the "human parts department".
3.  It is never boring - "How did the patient get THAT in THERE?"
4.  You get to do arts and crafts (Wax & Stains).
5.  Most important - Providing quality care to patients.

Cind Dubois
Integrated Pathology
Stockton, CA

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[Histonet] Histo Tech position in Brooklyn, NY - Long Island College Hospital

[Kyla Nemitz]

Hello Histonet! 

 

Long Island College Hospital (LICH), which serves as the hub of
Continuum's services in Brooklyn, is a 516-bed teaching hospital located
in the Brooklyn Heights/Cobble Hill section and is looking for a
Histology Technician to join its team! The ideal candidate for this
full-time position will have experience in Histology that includes:
grossing, special staining and embedding methodologies.  He/she will be
able to process tissue samples from fixation to paraffin, as well as use
a microtome to cut samples and prepare slides for staining.

 

Qualified candidates for this position must have:  

-  2-3 years experience working in a clinical lab.

-  At least an Associates Degree, preferably in Medical
Technology or related field.

-  A NYS certification as a Histology Technician or Technologist
(or eligible).

-  Strong communication (written and verbal) skills.

 

Additional Information:

Interested candidates apply online to:  www.chpnycareers.com
   

Continuum Health Partners is the non-profit hospital system that
supports Long Island College Hospital

*Continuum Health Partners is committed to diversity and equal
opportunity.

**No Agency Recruiters please

 

Feel free to contact me with any additional questions. Thank you! 

 

Kyla 

 

**

Kyla Nemitz

Recruiter - Long Island College Hospital 

Continuum Health Partners

555 W. 57th St

New York, NY 10019

Tel: (212)523-4050

Fax: (212)523-5776

knem...@chpnet.org

 

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RE: [Histonet] Freezing rodent brains for Mass Spec

[Gay Rudow]

We freeze mouse brains that have been perfused with 4% paraformaldehyde, put 
into PBS for 24 hours, and then into PBS with 30% sucrose overnight. We snap 
freeze them in methyl butane on dry ice. You have to put a thermometer into the 
methyl butane periodically to make sure that it doesn't get colder than -20 C 
or the brain will crack. Will the sucrose get in the way of what you are doing, 
or will this work for you?

Gay Rudow 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M 
O'Connor
Sent: Tuesday, April 14, 2009 6:54 PM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Freezing rodent brains for Mass Spec

I have a colleague who requires snap frozen  rodent brains to perform Mass 
Spec on 10micron frozen sections mounted on plates. Because OCT 
interferes with mass spec, they are currently using ethanol to slow the 
freezing process to prevent cracking of the brain in liquid nitrogen. The 
alcohol, however, causes some detriment to the surface of the brains - in 
science-speak "they get mushy".Does anyone have any suggestions for 
snap freezing brains without any substrate while preventing cracking? 

Jackie O'

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Re: [Histonet] Fli-1 antibody - Please help

[Richard Cartun]

We are now using an IVD-labeled antibody from BioCare with good results.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimens
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Young Kwun"  4/14/2009 11:06 PM >>>
Dear Histonetters,

I was wondering whether anyone had success in Fli-1 immunohistochemistry on
formalin-fixed, paraffin embedded human sections.

I tried two polyclonal, commercial antibodies from abcam (ab15289) and Santa
Cruz Biotech's Fli-1 (C-19, sc-356) without any positive stainings.

Both antibodies were recommended for application on paraffin sections.
Abcam's application notes even mentioned that perform enzymatic antigen
retrieval BEFORE commencing with HIER procedure. The procedure still did not
give any stains and the morphology was unacceptable. I tried with/without
HIER with different buffers as well.

I used primary dilutions 1:50 - 1:200 as recommended and I am using Leica's
BondMax Autostainer. 

Any comments would be appreciated.

 

Young

 

 

 

Young Kwun

Senior Hospital Scientist

Dept. of Anatomical Pathology

Concord Hospital 

Concord NSW 2139 Australia

Tel)61-2-9767-6075

Fax)61-2-9767-8427

kw...@email.cs.nsw.gov.au 

 

 

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[Histonet] Suggestions for alternatives to Envision+

[Webber, Mark]

We are looking to use an amplification method for DAB manual staining
and the DAKO envision+ had been recommended but as they are
discontinuing the small pack sizes this would be of no use to us. Does
anyone have experience with alternatives preferably from companies that
are easily accessed from Europe. 

One product that seemed a possibility is Invitrogen/Zymed SuperPicture
3rd Gen, does anyone have any experience with this product.

Thanks in advance for your help

 

Mark 

 

Senior Technical Officer

Department of Pathology
National University of Ireland, Galway
Clinical Science Institute
Costello Road
Galway 

 

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FW: [Histonet] decaling bones

[Ian Montgomery]

Hazel,
Asked the boys here in Anatomy about your question and according to
them if you're using a propriety decal agent you have to be careful as the
bones do get harder if they are left in the solution. The calcium comes out
but the bones are really tough to cut. Interestingly, it's not such a
problem if using EDTA or acid, just the propriety agents.
Ian. 

Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel
V
Sent: 14 April 2009 17:05
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decaling bones

We are having the hardest time decaling bones.   Femurs and tibias.

 

Is there such as thing as overdecaling and the bone becoming hard again?

 

Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's WaySlot 820

Little Rock, AR   72202

 

phone   501.364.4240

fax501.364.3155

 

visit us on the web at:www.archildrens.org

 









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[Histonet] Shur-Wave Processor

[JOSEPH FRAZEE]

   I would like some input on the Shur-Wave microwave system. I need to know 
pro's and con's. and is Isopropol alc. used on all processing. Thanks Histojoe
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[Histonet] More Lab Week trivia

[mtitford]

One year, everyone brought in photographs of their pets. There was a 
competition to guess which pets belonged to which lab employees. They say 
people look like their pets

Mike Titford
Pathology
USA
Mobile AL
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[Histonet] 5 Reasons - The list

[Cindy DuBois]

Thanks to so many of you I have come up with the following list:

1.  You get to dig into peoples brains like no psychologist can.
2.  Job security - no one else wants to work in the "human parts department".
3.  It is never boring - "How did the patient get THAT in THERE?"
4.  You get to do arts and crafts (Wax & Stains).
5.  Most important - Providing quality care to patients.

Cind Dubois
Integrated Pathology
Stockton, CA

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RE: [Histonet] Lab Week-Histology Trivial or Fun Facts

[Bernice Frederick]

All,
We actually brought in pictures of our parents one year for lab week. 
Another good one I saw was that someone snuck around and took pictures of
people's feet- it was wild.
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
stephanie.d.riv...@gsk.com
Sent: Tuesday, April 14, 2009 3:01 PM
To: Disher Lori
Cc: histonet@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] Lab Week-Histology Trivial or Fun Facts

Hi All,

No. I never heard the hard boiled egg sectioning fact.


1. Match the baby picture to the tech.

2. Gather information on if one weren't a histotech what would one do? 
Create a form and have the techs match names to the  profession. It 
could be fun. I participated in another lab once and the techs were funny. 
If I weren't a histotech I'd travel on the speech circuit. That particular 
person talked all day, literally. OR you can come up with professions 
based on the techs personality and have everyone guess on the form. Who 
ever get the most correct gets $5.00 Wawa card or something.

3. Unscramble laboratory terms from all labs including clinical.

4.Double check  this info, Who developed the diamond knife for electron 
microscopy AND improved the ultramicrotome
Answer: Humberto Fernandez-Moran--- Venezuela







Stephanie D. Rivera
Safety Assessment Department
GlaxoSmithKline
709 Swedeland RD
King of Prussia, PA 19406
phone: 610-270-7340
fax: 610-270-7202



"Disher Lori"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
14-Apr-2009 15:12
 
To
"histonet@lists.utsouthwestern.edu" 
cc

Subject
[Histonet] Lab Week-Histology Trivial or Fun Facts






Hello,
  I was wondering if anyone has some histo trivial-fun facts to share for 
Lab Week?  I remember a supervisor told me long ago that she was told 
while in training, that if you took a hard boilded egg and sectioned it at 
2 microns you would have enough sections to cover a football field.  Has 
anyone ever heard that one before?  Can anyone contribute any others?  We 
are trying to come up with some games for lab week.
Lori

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Re: [Histonet] (no subject)

[Rene J Buesa]

Some people do, others do not. I never used it more than once and, since they 
are quite expensive, that is why I prepared my own.
René J.

--- On Tue, 4/14/09, jeff  wrote:

From: jeff 
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, April 14, 2009, 11:55 AM

Is anyone using their target retrieval for dako more than 1 time?

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RE: [Histonet] tissue control bank NSH

[WILLIAM DESALVO]

Jodi,
Go to NSH.org site. The link at the
bottom takes you to this page. It can be found under About
NSH/Board/Committees/click on QC committee. 

http://www.e-guana.net/organizations.php3?action=printContentItem&orgid=111&typeID=1162&itemID=17799William
 DeSalvo B.S. HTL(ASCP)
Chair Person
Quality Control Committee
wdesalvo@hotmail.com

> Date: Wed, 15 Apr 2009 08:05:20 -0500
> From: putn...@ggclinic.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] tissue control bank NSH
> 
> Hi. I sent out a message before I thought to check with the NSH. I am a 
> member and went to the site but am not finding the info on the tissue control 
> bank. I tried to call but they are not open for another hour and I know that 
> I will forget to call back then and be lost in the work flow for the day. 
> Does anyone know how to access that on their site? I just need a little help 
> finding it if it is on there.
> Thanks. Sorry if it's obvious on the site and I missed it. I haven't had any 
> caffeine yet.
>  
>  
> Jodi Putnam (HT,ASCP)
> Graves Gilbert Clinic
> Pathology Department
> 201 Park Street
> Bowling Green, KY 42102
> (270) 393-2728 (voicemail)
> (270) 393-2795 
> Fax : (270) 393-2736
> _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
> 
> This email and any files transmitted with it are confidential and
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> states them to be the views of Graves-Gilbert Clinic.
> 
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RE: [Histonet] Liver (control tissue for PAS/D)

[Kemlo Rogerson]

I know this might be a stupid reply but as it's just for PAS/D can't you
use animal liver? Why not pig's liver? Go to an slaughter house and get
fresh pig's liver and bingo (I assume pig glycogen is the same as
our's?)... You could fry the residual with onions, very nice.




Kemlo Rogerson  
e-mail kemloroger...@nhs.net if not at work.
DD   01934 647057 or extension 3311 Mob 07749 754194; 
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah
Lehrer
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Putnam,
Jodi
Sent: 15 April 2009 13:56
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Liver (control tissue for PAS/D)

Hi again. I was wondering if anyone knew of a good source for liver
tissue for PAS/D control blocks. I am currently having to buy them and
it is ~42.00 for 10 slides. I am hoping to find a better price than that
as my doctor orders a fair amount of these. I don't have access to
autopsy tissue and when I did there was a problem with autolysis. I work
in a derm lab so I am limited. I have asked several of my former
employers and so far no luck. Any help acquiring the tissue or just
telling me about a better price with a different vendor would be great.
I will be totally out of tissue within ~2 weeks (if I am lucky to have
it last that long).
 
Thanks and everyone have a great day. Thanks to everyone for info for
manuals. I am so glad I signed up for the histonet.
 
Jodi
 
Jodi Putnam (HT,ASCP)
Graves Gilbert Clinic
Pathology Department
201 Park Street
Bowling Green, KY 42102
(270) 393-2728 (voicemail)
(270) 393-2795
Fax : (270) 393-2736
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
_ _

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[Histonet] tissue control bank NSH

[Putnam, Jodi]

Hi. I sent out a message before I thought to check with the NSH. I am a member 
and went to the site but am not finding the info on the tissue control bank. I 
tried to call but they are not open for another hour and I know that I will 
forget to call back then and be lost in the work flow for the day. Does anyone 
know how to access that on their site? I just need a little help finding it if 
it is on there.
Thanks. Sorry if it's obvious on the site and I missed it. I haven't had any 
caffeine yet.
 
 
Jodi Putnam (HT,ASCP)
Graves Gilbert Clinic
Pathology Department
201 Park Street
Bowling Green, KY 42102
(270) 393-2728 (voicemail)
(270) 393-2795 
Fax : (270) 393-2736
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

This email and any files transmitted with it are confidential and
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[Histonet] Liver (control tissue for PAS/D)

[Putnam, Jodi]

Hi again. I was wondering if anyone knew of a good source for liver tissue for 
PAS/D control blocks. I am currently having to buy them and it is ~42.00 for 10 
slides. I am hoping to find a better price than that as my doctor orders a fair 
amount of these. I don't have access to autopsy tissue and when I did there was 
a problem with autolysis. I work in a derm lab so I am limited. I have asked 
several of my former employers and so far no luck. Any help acquiring the 
tissue or just telling me about a better price with a different vendor would be 
great. I will be totally out of tissue within ~2 weeks (if I am lucky to have 
it last that long).
 
Thanks and everyone have a great day. Thanks to everyone for info for manuals. 
I am so glad I signed up for the histonet.
 
Jodi
 
Jodi Putnam (HT,ASCP)
Graves Gilbert Clinic
Pathology Department
201 Park Street
Bowling Green, KY 42102
(270) 393-2728 (voicemail)
(270) 393-2795 
Fax : (270) 393-2736
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

This email and any files transmitted with it are confidential and
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Re: [Histonet] BrdU + TUNEL, which one first

[Ana Resendes]

I used proteinase Kfor Tunel antigen retrieval...

2009/4/15 TF 

> Hi all, in many IHC co-labeling with BrdU, we perform the other antigen
> first.
> How about TUNEL?
> Do BrdU antigen retrieval (HCL or citrate buffer) affect TUNEL staining?
> Thanks very much.
>
> 2009-04-15
>
>
>
> TF
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-- 
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DVM, MSc, PhD, Veterinary Pathologist
Postdoctoral DRCT-fellow research scientist
Centro de Investigação de Recursos Naturais (CIRN)
Laboratório de Microscopia e Histologia
Departamento de Biologia, Universidade dos Açores.
Rua da Mãe de Deus, 58 - Apartado 1422
P - 9501-801 Ponta Delgada (Açores)
Portugal
Tel. (+351) 296 650 111
Fax (+351) 296 650 100
http://www.uac.pt/~pherg/
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RE: [Histonet] 5 Reasons

[Pamela Marcum]

You will create a work of art everyday that may save or extend a life.

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Wednesday, April 15, 2009 7:13 AM
To: Cindy DuBois; Histonet
Subject: RE: [Histonet] 5 Reasons

The Technical Staff in Pathology seem to fall into two types; those that
manage machines and computers and try to optimise their outputs. The
others use manual skills to create something, be it a slide, smear or
result from an agar plate (although I concede Micro is becoming
automated). I guess you choose the discipline that reflects your skills
and I guess the skills of a HistTech are manual dexterity, good hand eye
co-ordination and the ability to follow a procedure without deviation.
We don't have the machines, by and large, to automate our procedures
(except for H&E, Immunocytochemistry and many of the routine stains). I
guess one of the 5 is how you personally are 'wired'; are you a techy or
are you a craftsman. I don't believe that any of those genre to be
subservient to the other, just different skills. You could turn a
Microbiologist into a Histotech but can you turn a Chemist or a
Haematologist? As a Histotech that became a Cytologist (a morphologist)
and then a Manager, I'm interested in the skills that make us good (or
not) at what we do; I for one am still looking (g).


Kemlo Rogerson  
e-mail kemloroger...@nhs.net if not at work.
DD   01934 647057 or extension 3311 Mob 07749 754194; 
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah
Lehrer
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy
DuBois
Sent: 14 April 2009 16:52
To: Histonet
Subject: [Histonet] 5 Reasons

Please help me out here
I am suppose to post 5 Reasons to become a histotech in our lab.  It is
a competition for lab week.  Each department has to post 5 reasons you
should work in their department.

I am looking for some great answers
Thanks,
Cindy

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RE: [Histonet] 5 Reasons

[Kemlo Rogerson]

The Technical Staff in Pathology seem to fall into two types; those that
manage machines and computers and try to optimise their outputs. The
others use manual skills to create something, be it a slide, smear or
result from an agar plate (although I concede Micro is becoming
automated). I guess you choose the discipline that reflects your skills
and I guess the skills of a HistTech are manual dexterity, good hand eye
co-ordination and the ability to follow a procedure without deviation.
We don't have the machines, by and large, to automate our procedures
(except for H&E, Immunocytochemistry and many of the routine stains). I
guess one of the 5 is how you personally are 'wired'; are you a techy or
are you a craftsman. I don't believe that any of those genre to be
subservient to the other, just different skills. You could turn a
Microbiologist into a Histotech but can you turn a Chemist or a
Haematologist? As a Histotech that became a Cytologist (a morphologist)
and then a Manager, I'm interested in the skills that make us good (or
not) at what we do; I for one am still looking (g).


Kemlo Rogerson  
e-mail kemloroger...@nhs.net if not at work.
DD   01934 647057 or extension 3311 Mob 07749 754194; 
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah
Lehrer
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy
DuBois
Sent: 14 April 2009 16:52
To: Histonet
Subject: [Histonet] 5 Reasons

Please help me out here
I am suppose to post 5 Reasons to become a histotech in our lab.  It is
a competition for lab week.  Each department has to post 5 reasons you
should work in their department.

I am looking for some great answers
Thanks,
Cindy

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RE: [Histonet] Lab Week-Histology Trivial or Fun Facts LONG OFF TOPIC

[Kemlo Rogerson]

I can only say Pathology needs more kids like you. I've no idea what your 
saying but it looks very interesting...


Kemlo Rogerson  
e-mail kemloroger...@nhs.net if not at work.
DD   01934 647057 or extension 3311 Mob 07749 754194; 
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer
This e-mail is confidential and privileged. If you are not the intended 
recipient please accept my apologies; please do not disclose, copy or 
distribute information in this e-mail or take any action in reliance on its 
contents: to do so is strictly prohibited and may be unlawful. Please inform me 
that this message has gone astray before deleting it. Thank you for your 
co-operation

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
koelli...@comcast.net
Sent: 15 April 2009 05:02
To: Disher Lori
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Lab Week-Histology Trivial or Fun Facts LONG OFF TOPIC



Hi in grad school taking microanatomy and pathology classes, 2 that I heard are 
this:  The surface area of all the alveoli in the lungs of an adult is between 
40-70 square meters. That seems reasonable in having a 40-70 square meter 
surface (where all gas exchange takes place) represent all the gas exchange in 
lungs. Have seen that figure numerous times so while can't test it, can believe 
it.  The other one that I also can't test and is hard to believe is that the 
sum total length of all vessels (large small, artery vein down to every single 
capillary) in one adult measures about 100,000 kilometers (62,000 miles). Again 
there are many disparate medical and anatomical references so either all right 
or all wrong. 



The 2 micron sectioned egg I don't believe. (1) There are 25,400 microns in an 
inch. A 2 inch long egg is about 50,000 microns long. At 2 microns per section 
thats about 25,000 egg sections.  Even is each section is 2 square inches 
(that's generous since each end isn't close to 2 squre inches in area), thats 
100,000 square inches. At 1,296 square inches per square yard, that's about 40 
square yards which is far short of a football field (100 yards x 53 yards). (2) 
If you calculate the volume of a "solid rectangle" covering a football feild 
that is 100 yards x 53 yards x 2 microns and of course converting all to yards 
or microns, the answer is a specific volume.  If you take the volume of an 
ellipsoid which is four thirds times pi times a times b times c with a, b and c 
being the lenggth of the 3 axis of the ellipsoid, and using approximate 
measurements for the egg, I come up with far , far less volume in egg than in 
the "rectangular solid" covering football field. (3) This is a classical 
calculus definte integral washer problem. Whether this egg as an ellipsoid is 
scalene, oblate or prolate, integrating volume over the limits of integration 
gives me much, much less volume than is needed to cover a football field 2 
microns thick.  Have tried all 3 methods and converting everything to  microns 
or yards using scientific notation. So 6 calculations.  Everytime I come up 
somewhere close to the area of 2 micron slices covering approximately 1/100 of 
the football field. 



Unless my math is all wrong, or this is a humongous, enormous ostrich and not 
chicken egg. 



Ray 

Raymond Koelling 

PhenoPath Labs 

Seattle, WA 


- Original Message -
From: "Disher Lori" 
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, April 14, 2009 12:12:40 PM GMT -08:00 US/Canada Pacific
Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts 

Hello,
  I was wondering if anyone has some histo trivial-fun facts to share for Lab 
Week?  I remember a supervisor told me long ago that she was told while in 
training, that if you took a hard boilded egg and sectioned it at 2 microns you 
would have enough sections to cover a football field.  Has anyone ever heard 
that one before?  Can anyone contribute any others?  We are trying to come up 
with some games for lab week. 
Lori 

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[Histonet] Antibody for ZP Proteins

[Nejat Yilmaz]

Dear Colleagues,

We're planning to study ZP1, ZP2 and ZP3 protein immunohistochemistry on 
mice ovary. We couldn't find commercially available antibodies for these 
proteins suitable for IHC. Does anybody know sources for buying this items?

Thanks in advance...

Dr. Necat Yilmaz MD, PhD
University of Mersin 



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[Histonet] Leica TP 1020 processor

[Edwards, R.E.]

Does  anybody  know  anything good  or  bad  even about  the  above  tissue  
processor, for  example  can  it  be  used on  the  open bench?, without  any  
external extraction.
 Many  thanks

 Richard  Edwards
  Leicester University...U.K.

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[Histonet] BrdU + TUNEL, which one first

[TF]

Hi all, in many IHC co-labeling with BrdU, we perform the other antigen first.
How about TUNEL?
Do BrdU antigen retrieval (HCL or citrate buffer) affect TUNEL staining?
Thanks very much.

2009-04-15 



TF 
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