Re: Re: [Histonet] Re: IHC on frozens
it is because when mentioning frozen section, we r talking about different procedures. some fix brain, sucrose sink, then perform OCT embedding and cryostat section;you can use enzyme digestion or HIER for antigen retrieval some other use fresh brain, snap freezing, and cryostat section; this approach does not require HIER. 2009-07-07 TF 发件人： Colleen Forster 发送时间： 2009-07-07 02:41:59 收件人： Johnson, Teri 抄送： histonet@lists.utsouthwestern.edu 主题： Re: [Histonet] Re: IHC on frozens I disagree. I do heat retrieval on frozen sections that have been fixed in a formalin fix. If you use the correct protocol you can get very nice IHC on these. Colleen Forster HT(ASCP)QIHC Anatomic Pathology Research Laboratory U of MN Johnson, Teri wrote: In response to this thread: snip Kimberly: Your question has a two parts answer: 1- it cannot be done because the sections will peel off, but most importantly 2- it is not necessary since HIER was developed to undo the cross linkage produced by the NBF fixation, and the tissues used for?FS are not fixed. ren? J.? --- On Thu, 7/2/09, Kimberly Tuttle ktut...@umm.edu wrote: From: Kimberly Tuttle ktut...@umm.edu Subject: [Histonet] IHC on frozens To: histonet histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 12:55 PM Can you do heat retrieval on frozens? Kimberly C. Tuttle? HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. snip I disagree that you cannot do HIER on frozen sections. We do them all the time. All our samples are fixed in formalin, and then cryoprotected in sucrose prior to freezing them, so providing an antigen retrieval step usually produces good IHC results in frozen sections. We section and dry the slides, then use citrate buffer pH 6.0 in the microwave at 60 degrees C for 10 minutes, cool 10 minutes, then rinse and continue with IHC protocol. Use Plus slides and keep the solution under boiling temperature and you should be fine. For things like brain or bone, you might want to use lower temperatures for longer period of time instead of high temps for less time. It can even work in fresh-frozen samples which are sectioned and then fixed prior to immunostaining. Some great information on this is included in this article: Yamashita and Okada, Application of Heat-induced Antigen Retrieval to Aldehyde-fixed Fresh Frozen Sections, JHC Vol 53(11): 1421-1432, 2005. A big thanks to Gayle Callis for the heads up on this paper! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tween 20
Hi all, What is the difference between Enzyme Grade Tween 20 and Molecular Biology Grade Tween 20? Thanks! Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Accu-edge sales rep
I've had the same problem. I started keeping them in a ziplock with desiccant packets and it seems to help. Even then I have to bang them top-down to advance the blades to the top so they catch on to the slider mechanism. I don't know why I put up with this - except that I'm awfully busy doing Histology!! Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Mon 7/6/2009 3:33 PM To: histo...@pathology.swmed.edu Subject: [Histonet] Accu-edge sales rep Hello, I just opened a brand new box of Accu-Edge high profile blades and I cannot get the very first blade to slide out of the container. Even though I have had the box of unopened blades for some time, I was wondering if you can guide me in some way that I can retrieve these blades without cutting myself or be able to compensate me with a new box and I would gladly return the box of sticky blades. I would greatly appreciate your help. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tween 20
Ask the manufacturer, they will know. Or look at the MSDS Geoff Merced M Leiker wrote: Hi all, What is the difference between Enzyme Grade Tween 20 and Molecular Biology Grade Tween 20? Thanks! Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Digital Slide Imaging
We had our set of instructional slides for Histology (medical student course) scanned at 20X and are very happy with the results. Students load the slides and viewing program on their laptops and study wherever. Higher mag scanning (40X) is necessary for finer cellular detail. I don't know if even 40X would be sufficient for hematology. Geoff Tiana Fountain wrote: Hi Everyone, What are the current thoughts on digital slide imaging systems like Aperio, etc. I am interested in other people's experience with slide imaging systems and their preferences. Any opinions/suggestions would be appreciated! Tiana Baskin This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Accu-edge sales rep
I had a box of low profile blades with the same problem. The vendor sent me a new box. Geoff DiCarlo, Margaret wrote: Hello, I just opened a brand new box of Accu-Edge high profile blades and I cannot get the very first blade to slide out of the container. Even though I have had the box of unopened blades for some time, I was wondering if you can guide me in some way that I can retrieve these blades without cutting myself or be able to compensate me with a new box and I would gladly return the box of sticky blades. I would greatly appreciate your help. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Management Job Alert 7/7/09 Histology Supervisor/Manager needed in San Antonio, TX
Hi Histonetters!! I hope everyone had a safe and happy 4th of July weekend. I have a new position that I am very excited about and wanted to let you know about. This is a full time permanent position with one of my best clients. They offer excellent salary, benefits and relocation/sign on bonus. My client is a private pathology lab and they are in need of a shift production supervisor. This position offers an excellent opportunity for growth and expansion. My client is looking for someone with big lab production skills who is good with managing people and workflow. No administrative except keeping production and QS records. If you or anyone you know might be interested in this position please contact me I can be reached at rel...@earthlink.net or toll free at 866-607-3542. Have a great day!! Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: rel...@earthlink.net mailto:rel...@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alk-1 controls
Does anyone have a good source to purchase ALK-1 IHC controls from? We were getting them from Cell Marque, but their source is no longer available!! Thanks for any help!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Error corrections
How are people typically correcting errors that occur. For example, mislabeled slides and path slips. We do a lot of hand writing in our lab and when we make a mistake people are usually whiting out or scribbling. I'm feeling like we shouldn't be doing thisis there a standard like a line through the error with the person's initials or something? The other thing is that we do all the grossing (Derm) and we do not dictate, we draw a picture of the specimen and write down the measurements. If the picture is wrong people white it out and re-draw it. I hope I'm making sense. Any suggestions? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hemo-d xylene substitute in processor
Hi everyone. I was recently contracted to run a small routine derm lab. This is the first lab i've seen a lab use hemo-d as a xylene substitute. Are there any properties of this I should keep in mind while using it in processing / staining. Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CoPath Question
Periodically, we receive a call from an angry physician who wants to know why the specimen he or she sent to us has not been signed out. For those of you using CoPath as your Anatomic Pathology LIS, do you have a protocol (or system) for identifying cases that have not been signed out? We run reports for each pathologist to identify these cases, but, for some reason, we miss some cases. Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] methylene blue microinjection
Dear All, Does anyone use intracerebroventricular methylene blue injection for the validation of drug/electrode injection/placement accuracy? Could you please give me the catalogue number of methylene blue, so I can order for myself? And any other advices would be greatly appreciated. Thank you very much in advance. Salim syi...@ucalgary.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ventana IHC and special stains users
Please for those of you using a Ventana IHC instrument and/or special stains instrument, I am interested in what you do to dispose of your toxic waste. I know each municipality is different so let me know what you do and where you live. Ventana does not separate toxic from non toxic waste so I imagine there is a lot of toxic waste to deal with and the liquid coverslip alone is something that I would think should not go down the drain?? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
