[Histonet] Any help for this oil red o paper
Dear histonetters, Can anybody will help me with the following oil red o paper from Charles Churukian. Journal of Histotechnology, 22(4):309-311 Charles Churukian, Lillie's Oil Red O method for neutral lipids. I am not able to access the NSH site,the following error message is coming. (ERROR: Specified ROOT dir [/var/www/html/live/tools/htdocs/newsite_templates/] is not a directory) Thanks abijag Looking for local information? Find it on Yahoo! Local http://in.local.yahoo.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] anyone tried to get onto the NSH website today?
Good morning everyone, I have tried several times to get onto the NSH website today and I keep getting an error. I am wondering if it is down or if there is something wrong on my end. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part Time Position In Surprise, AZ
Good Morning All, I have an immediate opening for a Histotechnician/Histotechnologist candidate in Surprise, AZ. The position would require the qualified candidate to work 2 days/week. 7:30am-5:00pm. HT ASCP is required, and 2+ years experience required as well. To apply please send an updated copy of your resume for prescreening purposes, *All inquires are kept confidential* following resume submitted you will be contacted to set up an initial phone screen. Thank you in advance for any inquiries. :-) -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH Website Down?
I just tried the NSH website, and it seems to be down so it must not be us... J I'm sure someone has contacted them. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Any help for this oil red o paper
Try http://www.urmc.rochester.edu/path/zqu/StainsManual/index.html?OILREDOMETHODFORFATS This is from the latest version of Chuck Churukian's lab manual; I think the method is the same as in his 1999 paper in J. Histotechnol. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: abi jag abija...@yahoo.co.in Date: Monday, July 20, 2009 7:30 Subject: [Histonet] Any help for this oil red o paper To: histonet@lists.utsouthwestern.edu Dear histonetters, Can anybody will help me with the following oil red o paper from Charles Churukian. Journal of Histotechnology, 22(4):309-311 Charles Churukian, Lillie's Oil Red O method for neutral lipids. I am not able to access the NSH site,the following error message is coming. (ERROR: Specified ROOT dir [/var/www/html/live/tools/htdocs/newsite_templates/] is not a directory) Thanks abijag Looking for local information? Find it on Yahoo! Local http://in.local.yahoo.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] anyone tried to get onto the NSH website today?
As of 11:30 CDT the NSH site is up and running or at least I did not have a problem getting it to come up. Freida Carson --- On Mon, 7/20/09, Kim Merriam kmerriam2...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] anyone tried to get onto the NSH website today? To: Histonet histonet@lists.utsouthwestern.edu Date: Monday, July 20, 2009, 7:46 AM Good morning everyone, I have tried several times to get onto the NSH website today and I keep getting an error. I am wondering if it is down or if there is something wrong on my end. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] marilyn weiss will be out of the office
I will be out of the office starting 07/20/2009 and will not return until 07/21/2009. I will respond to your message when I return.In my absence please ask for Mary Campbell or Laurie at 619-528-6801 if this is urgent they can contact me or give you my cell phone number. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fluorescent dyes and xylene
Will the Alexa Fluor dyes withstand some dehydration and clearing in alcohol and xylene? If they won't are there any bright fluorescent dyes that can be obtained congugated to a secondary antibody that will? Looking to immunostain tyrosine hydroxylase in rodent brain with a fluorescent marker in tissue that needs to be dehydrated and cleared, perhaps with an abbreviated series. Bob Nienhuis VA / UCLA Neurobiology Research ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fluorescent dyes and xylene
So you are saying that you cannot just mount the brain tissue in aqueous mounting medium at the end (after washing the excess secondary antibody)? You must dehydrate and clear before mounting (in a permament medium)? Just curious... Merced --On Monday, July 20, 2009 10:37 AM -0700 Bob Nienhuis bob.nienh...@gmail.com wrote: Will the Alexa Fluor dyes withstand some dehydration and clearing in alcohol and xylene? If they won't are there any bright fluorescent dyes that can be obtained congugated to a secondary antibody that will? Looking to immunostain tyrosine hydroxylase in rodent brain with a fluorescent marker in tissue that needs to be dehydrated and cleared, perhaps with an abbreviated series. Bob Nienhuis VA / UCLA Neurobiology Research ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HELP Starting a lab
I have a friend who is a Pathologist and starting a new lab. She sent me a list of start up supplies and equipment that she needs to purchase. Can I get some input on whats missing from her list, I've added a few things but Im going blank. Its a hisology lab for a urology practice if that helps. Thank you in advance! PLEASE VENDORS DONT CALL OR EMAL ME Equipment: Negative pressure hood for grossing of specimens (grossing station) Refrigerator w/freezer or separate freezer Cassette labeler Tissue processor Embedding Stations Microtomes Waterbaths, 1 per microtome Automatic HE stainer A scale and a mixing/heating plate Manual staining dishes, and slide holders that fit in them. Slide and block storage system Supplies: Safety equipment like goggles and sleeve covers, aprons, a flame cabinet for flammable liquids acid cabinet? Blades Forceps Rulers Hemostats Scissors Glassware like flasks, beakers, graduated cylinders, and a funnel.Pipettes and tips. Reagents: Formalin 70% etoh 95% etoh 100% etoh xylene paraffin Hematoxylin stain Eosin stain Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Fluorescent dyes and xylene
Sorry, I should have mentioned that this will be done on thick frozen sections. Bob On Mon, Jul 20, 2009 at 10:37 AM, Bob Nienhuis bob.nienh...@gmail.comwrote: Will the Alexa Fluor dyes withstand some dehydration and clearing in alcohol and xylene? If they won't are there any bright fluorescent dyes that can be obtained conjugated to a secondary antibody that will? Looking to immunostain tyrosine hydroxylase in rodent brain with a fluorescent marker in tissue that needs to be dehydrated and cleared, perhaps with an abbreviated series. Bob Nienhuis VA / UCLA Neurobiology Research ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Fluorescent dyes and xylene
Hello My experience is that most Immunofluorescence dyes do not withstand alcohol and organic solvents dehydration. You can use aqueous mounting medium with anti-fading agent. Frozen brain tissue is tough to work with; since it contains tons of lipids. Lots of mounting mediums will produce air bubbles because aqueous mounting medium being hydrophilic and brain tissue being hydrophobic. We are in the process of developing an aqueous mounting medium with anti-fading agent and should be OK to use with tissue like brain, if interested please let us know we can send you a sample for evaluation. Bader On Mon, Jul 20, 2009 at 11:22 AM, Bob Nienhuis bob.nienh...@gmail.comwrote: Sorry, I should have mentioned that this will be done on thick frozen sections. Bob On Mon, Jul 20, 2009 at 10:37 AM, Bob Nienhuis bob.nienh...@gmail.com wrote: Will the Alexa Fluor dyes withstand some dehydration and clearing in alcohol and xylene? If they won't are there any bright fluorescent dyes that can be obtained conjugated to a secondary antibody that will? Looking to immunostain tyrosine hydroxylase in rodent brain with a fluorescent marker in tissue that needs to be dehydrated and cleared, perhaps with an abbreviated series. Bob Nienhuis VA / UCLA Neurobiology Research ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne / 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience corp. Phone: + 425 367 4601 Phone: + 425 514 3761 Fax: + 425 367 4817 cell (mobile) phone: + 425 314 0199 e-mail address: bade...@gmail.com Web site: www.immunobioscience.com Marketing: phone: + 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HELP Starting a lab
Pretty good list. Don't forget first aid kits and spill kits. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Mon, 20 Jul 2009 14:26:57 -0400 From: ktut...@umm.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP Starting a lab I have a friend who is a Pathologist and starting a new lab. She sent me a list of start up supplies and equipment that she needs to purchase. Can I get some input on whats missing from her list, I've added a few things but Im going blank. Its a hisology lab for a urology practice if that helps. Thank you in advance! PLEASE VENDORS DONT CALL OR EMAL ME Equipment: Negative pressure hood for grossing of specimens (grossing station) Refrigerator w/freezer or separate freezer Cassette labeler Tissue processor Embedding Stations Microtomes Waterbaths, 1 per microtome Automatic HE stainer A scale and a mixing/heating plate Manual staining dishes, and slide holders that fit in them. Slide and block storage system Supplies: Safety equipment like goggles and sleeve covers, aprons, a flame cabinet for flammable liquids acid cabinet? Blades Forceps Rulers Hemostats Scissors Glassware like flasks, beakers, graduated cylinders, and a funnel.Pipettes and tips. Reagents: Formalin 70% etoh 95% etoh 100% etoh xylene paraffin Hematoxylin stain Eosin stain Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Windows Live™ Hotmail®: Search, add, and share the web’s latest sports videos. Check it out. http://www.windowslive.com/Online/Hotmail/Campaign/QuickAdd?ocid=TXT_TAGLM_WL_QA_HM_sports_videos_072009cat=sports___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Celloidin Sections.
I am curious--How thin can celloidin sections be cut? I've heard that 30 microns is a standard thickness. No way to make 5 micron sections? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology _ Windows Live™ SkyDrive™: Store, access, and share your photos. See how. http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Celloidin Sections.
Jerry depends on whether you are talking about celloidin or Low Viscosity Nitrocelulose. There are techniques in the literature to cut celloidin sections as thin as 4 microns. For LVN you need much thicker sections as this plastic is much less sturdy and has a tendency to fragment if too thin. The questions I ask is why you need celloidin sections and what tissue are you thinking about for this technique? Barry From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JR R [rosenfeld...@hotmail.com] Sent: Monday, July 20, 2009 6:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Celloidin Sections. I am curious--How thin can celloidin sections be cut? I've heard that 30 microns is a standard thickness. No way to make 5 micron sections? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology _ Windows Live™ SkyDrive™: Store, access, and share your photos. See how. http://windowslive.com/Online/SkyDrive?ocid=TXT_TAGLM_WL_CS_SD_photos_072009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] double immunostaining with antigen retrieval and no antigen retrieval
Dear All, Is it possible to do double immunostaining for one antibody which doesn't require antigen retrieval, and the other one which doesn't need it. Can you do the one without retrieval first, then retrieve and do the other one, or does the retrieval damage the first staining? We know that doing antigen retrieval for antibody that doesn't require it gives us non specific staining. I would appreciate some advice. Thank you very much. Cathy Malcontenti-Wilson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
