RE: [Histonet] histology for kids
Depends on the age of the kids as I don't understand the term 'grade-school'. What I did for kids around 10 yesrs old or so was to go to the Butchers and get some Ox kidney, heart and liver. I prepared slides from them, took a microscope to let them see the structure and also took scapels for them to cut up the animal tissue. Odd how many kids haven't handled animal organs or raw meat. Anyways be careful of the scapel maybe you risk adverse Americans ought just to use scissors or a pen knife. Ask the kids they might be carrying a blade!! (joke, joke, honest). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: 22 July 2009 14:58 To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen HE
Hello Histo folks I am having a hard time with consistency with my frozen HE. It was always to the Pathologist liking till the last year. I have not changed stains or reagents so I am not sure what may be the problem. Would anyone care to share with me their frozen section HE procedure? Maybe I just need to change the whole process. I do filter and change the stains weekly and reagents as needed, so I am not sure what else to try. Thanks in advance for the help Kimberly Marshall HT (ASCP) == The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cyclin D3
Hello All, Is anyone out there currently doing Cyclin D3? We are having trouble getting any staining at all. We are doing manual heat retrieval and staining. Any suggestions or protocols would be appreciated. Thanks in advance, Carol Carol Wilson, HT(ASCP) Lead Technician/Histology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
well...maybe it's just research labs at UB then...i guess we're pretty old school! --On Wednesday, July 22, 2009 7:03 PM -0400 Blazek, Linda lbla...@digestivespecialists.com wrote: Hay! Another one of us oldies! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, July 22, 2009 6:07 PM To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] who owns microm these days?
Yes, ThermoFisher Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, July 23, 2009 7:52 AM To: Histonet Subject: [Histonet] who owns microm these days? is it ThermoFisher? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
Ah, yes. The '70's. Our lab had 2 windows, both on the same side of the wall. The pathologists cut under one of the windows and it had one of those box fans, pointing out. In Michigan. No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared. Ah..those were the days?? Although I miss it, I'm glad I had to give up my coffee and donut when cutting. Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!) Progress is good. But I still like my '70's music!!! Bob Seger and old motown!!! ;) Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do. -Original Message- From: Edwards, R.E. [mailto:r...@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
RE: [Histonet] Start Up Lab
Thanks to spell-check..it self-corrected me..incorrectly! Thanks for setting it right. -Original Message- From: Smith, Allen [mailto:asm...@mail.barry.edu] Sent: Thursday, July 23, 2009 12:26 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Start Up Lab I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do. -Original Message- From: Edwards, R.E. [mailto:r...@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However,
RE: [Histonet] Peloris tissue processor
We have had our Peloris since Feb. and we love it. We use the 6 hour program for our regular tissue and the 8 hour program for breast, thyroid, colon, melanoma, sentinel nodes, prostatectomy. The one hour program for prostate biopsies. We use Formula 83 instead of Xylene, or you can use Xylene free processing using Isopropyl alcohol. We like being able to run both retorts at the same time, of course they don't finish at the same time but closely. Your reagent usage drops also. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Wednesday, July 22, 2009 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris tissue processor Anyone currently using the Peloris tissue processor please comment- love it, likes, dislikes? Thank you in advance for your input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
In the 80's I worked in a drug screening lab for the Navy and one step in the extraction involved fuming HCl (meaning pure HCl). Several of the techs got nose bleeds every time they used the stuff. I finally pried open a window (they were painted shut) and we turned on a fan the size of a propeller to blow the fumes out the window. We used Xylene in a room with no ventilation and found out that high concentrations of xylene cause transient euphoria. No wonder we thought every thing was funny during those days. Safety people, they take all the fun out of research! ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: RE: [Histonet] Start Up Lab To: cb...@lexclin.com, montina.vanme...@pbrc.edu, theci...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 3:53 PM Ah, yes. The '70's. Our lab had 2 windows, both on the same side of the wall. The pathologists cut under one of the windows and it had one of those box fans, pointing out. In Michigan. No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared. Ah..those were the days?? Although I miss it, I'm glad I had to give up my coffee and donut when cutting. Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!) Progress is good. But I still like my '70's music!!! Bob Seger and old motown!!! ;) Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing
RE: [Histonet] Start Up Lab
You might still have your fingers, but are your fingerprints still the same? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov wrote: From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov Subject: RE: [Histonet] Start Up Lab To: Smith, Allen asm...@mail.barry.edu Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 4:32 PM Thanks to spell-check..it self-corrected me..incorrectly! Thanks for setting it right. -Original Message- From: Smith, Allen [mailto:asm...@mail.barry.edu] Sent: Thursday, July 23, 2009 12:26 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Start Up Lab I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do. -Original Message- From: Edwards, R.E. [mailto:r...@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot.
RE: [Histonet] Start Up Lab
I went to the Navy's MLT program in 79-80 and we did drug screens manually and the chemicals that we used would give us a buzz. Thought it was ironic that I was getting high while looking for drugs in somebody else. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello [vapat...@yahoo.com] Sent: Thursday, July 23, 2009 1:30 PM To: Lynette Pavelich; HistoNet Subject: RE: [Histonet] Start Up Lab In the 80's I worked in a drug screening lab for the Navy and one step in the extraction involved fuming HCl (meaning pure HCl). Several of the techs got nose bleeds every time they used the stuff. I finally pried open a window (they were painted shut) and we turned on a fan the size of a propeller to blow the fumes out the window. We used Xylene in a room with no ventilation and found out that high concentrations of xylene cause transient euphoria. No wonder we thought every thing was funny during those days. Safety people, they take all the fun out of research! ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images flow through our CRIC. --- On Thu, 7/23/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: RE: [Histonet] Start Up Lab To: cb...@lexclin.com, montina.vanme...@pbrc.edu, theci...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 23, 2009, 3:53 PM Ah, yes. The '70's. Our lab had 2 windows, both on the same side of the wall. The pathologists cut under one of the windows and it had one of those box fans, pointing out. In Michigan. No hoods, an old duo-autotechnicon and xylene in full use with disposal down the drain. Every day, I would go home not knowing if I went thru a red light or not, arriving home , head still cloudy for about 30 minutes until it cleared. Ah..those were the days?? Although I miss it, I'm glad I had to give up my coffee and donut when cutting. Treats in the drawer are gone, mouth pipetting is gone (not mine, but yes, the mouth does turn black from silver nitrate!!) Progress is good. But I still like my '70's music!!! Bob Seger and old motown!!! ;) Montina Van Meter montina.vanme...@pbrc.edu 07/23/09 11:31 AM I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] Histotech needed
Greensboro Pathology, a leading provider of pathology services for 50+ years, is currently seeking an experienced Histotechnician to work in a Chapel Hill satellite office. The Histotechnician will participate in the full cycle of dermatopathology specimen preparation from grossing through sectioning and staining. Some travel to Greensboro during initial training will be required. Requirements: *Current HT/ASCP certification preferred. *Previous sectioning staining experience required. *Grossing experience preferred, but will train an otherwise qualified candidate. *Must have a minimum of 24 credit hours in biology, chemistry, laboratory science (or a combination thereof) to qualify for grossing tissue. Proof of credits will be required. *Effective communication skills are required. *Must be able to work independently. Please forward resume/CV to: Greensboro Pathology Attn: HR Director PO Box 13508 Greensboro, NC 27415 Fax: 336/510-0192 epanco...@gsopath.com www.greensboropathology.com Equal Opportunity Employer Drug Free Workplace Kathy S. Hart Histology Manager Greensboro Pathology, LLC 706 Green Valley Rd. Suite 104 Greensboro, NC 27408 kh...@gsopath.com phone: 336-387-2513 fax: 336-387-2563 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Like specimens
Any new and innovative ways to differentiate like specimens at grossing? I know that there are specialty labs (GI; prostate, etc) out thee. How do you not gross like specimens back-to-back? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Like specimens
We never assession like tissues back-to-back. A different type of specimen always goes between like tissues. (except in the Dermatopathology area...) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Nita Searcy Sent: Thu 7/23/2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Like specimens Any new and innovative ways to differentiate like specimens at grossing? I know that there are specialty labs (GI; prostate, etc) out thee. How do you not gross like specimens back-to-back? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Like specimens
We have no choice other than to put prostates back to back. To avoid mix up we use specimen inking. Our paper on the subject is in Archives of Pathology http://arpa.allenpress.com/arpaonline/?request=get-abstractdoi=10.1043% 2F1543-2165-133.2.295 Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Thursday, July 23, 2009 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Like specimens Any new and innovative ways to differentiate like specimens at grossing? I know that there are specialty labs (GI; prostate, etc) out thee. How do you not gross like specimens back-to-back? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 __ Information from ESET NOD32 Antivirus, version of virus signature database 4270 (20090723) __ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __ Information from ESET NOD32 Antivirus, version of virus signature database 4270 (20090723) __ The message was checked by ESET NOD32 Antivirus. http://www.eset.com BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Inking article
I have received several requests for a better link to our inking article. The article title is: Lester J. Raff MD, George Engel MD, Kenneth R. Beck MD, Andrea S. O'Brien HT, ASCP and Meagan E. Bauer BA. 2009: The Effectiveness of Inking Needle Core Prostate Biopsies for Preventing Patient Specimen Identification Errors: A Technique to Address Joint Commission Patient Safety Goals in Specialty Laboratories. Archives of Pathology and Laboratory Medicine: Vol. 133, No. 2, pp. 295-297. A link that should work is: http://arpa.allenpress.com/pdfserv/10.1043%2F1543-2165-133.2.295 Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Benchmark XT for sale
Hello HistoNetters We are selling our Ventana Benchmark XT IHC stainer. If anyone is interested, please see the ad on our web site. http://www.sarapath.com/equipment4sale/ I would be happy to answer any questions, please feel free to email or contact me directly. Thank You Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology (941) 362-8963 (941) 362-8964 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vet tissue processing
I'm looking for a histology processing lab who will process NBF veterinary tissues to H+E slides. I need a short turn around time, like 7-10 days. Any suggestions will be considered. Thanks, **Dell Deals: Treat yourself to a sweet deal on popular laptops! (http://pr.atwola.com/promoclk/100126575x1223100673x1201716527/aol?redir=http:%2F%2Faltfarm.mediaplex.com%2Fad%2Fck%2F12309%2D81939%2D1629%2D7) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] alarms on all the machines
The other day just about every machine in the lab decided to beep and alarm all at the same time. They all sound the same!!! The stainers in histo and cyto. The processors that they were done cleaning. The alarms on the freezers ( been open too long) Both of the Venatana IHC machines and special stain timers. Plus there was a truck backing up to the door to unload something and it to was beeping. So we were wondering since cell phones have all types of different rings and tones to them couldn't someone change the beeping to something else or give us a choice. Everyone decided that I should post this on the histo net to see what everyone else thought or maybe you guys that design these machines (yes they are all wonderfull) but gives us some more sound options. Just a thought! Okay, so what does everyone think about this? Connie _ Windows Live™ Hotmail®: Celebrate the moment with your favorite sports pics. Check it out. http://www.windowslive.com/Online/Hotmail/Campaign/QuickAdd?ocid=TXT_TAGLM_WL_QA_HM_sports_photos_072009cat=sports___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
