[Histonet] NMDAR c-fos Abs

2009-09-12 Thread linresearch


Hello, 

Dose anyone know of reliable souces for the 2 ABs above that give consistnet 
results? 

I need to use them the NMDAR1 Ab on PFFE rat tissues and the c-fos on either 
frozen 

or PFFE tissues. 

I have tried one NMDAR1 ab with inconsistent results, one batch works well and 
the next 

batch does not give the same results using the same protocol. 

Thanks, 

lin 
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[Histonet] Re: mercury vs led lights **Commercial Response**

2009-09-12 Thread Hobbs, Carl


Thanks for adding a response.
However, I am a old Techie who does not understand what you are saying.
My apologies.
 I use Alexa 488, 594 and also Hoechst to stain nuclei ( red, green , blue)
So, if I changed from MY  Xcite system,  to LEDs, can I be assured that those 
three flourochromes I use will be equally detectable?
What would I need to do to change to LEDs?
What do I gain?

I would be most grateful for further info, in easy language..you are very 
welcome to email me personally.
Thanks.


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RE: [Histonet] formalin storage

2009-09-12 Thread Podawiltz, Thomas
I think the 1 gallon per 100 square feet is OSHA.  What you have to make sure 
not to exceed the amount your cabinet is rated. for. We do not store are 10 % 
in a flammable cabinet.



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Tina J. 
[tina.ha...@va.gov]
Sent: Friday, September 11, 2009 9:37 AM
To: Jean Warren; Riesen, Rebecca; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] formalin storage

WE do not store our 10 NBF in flammable storage.

However, Could you tell me where you found this direction of storing
only 1 gallon per 100 sq. feet in a flammable cabinet, and 1 gallon
outside?  More specific than NFPA.  I have been unable to find that
anywhere, our safety folks tell us that if they are in a flammable
cabinet the amount per room size doesn't matter.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean
Warren
Sent: Friday, September 11, 2009 9:25 AM
To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] formalin storage

No, it is ridiculous. Safety people tried to argue this with us years
ago.
One of our pathologists told them, How can something that is almost 90%

water be a combustion hazard?


- Original Message -
From: Riesen, Rebecca rebecca.rie...@nchmd.org
To: histonet@lists.utsouthwestern.edu
Sent: Friday, September 11, 2009 9:15 AM
Subject: [Histonet] formalin storage




We have been directed by our Safety Officer to store all formalin (37%
and 10% NBF) in a flammable storage room, cabinet or container.  Yes,
37% Formalin we do store in this manner, but I have never heard of this
requirement for 10%NBF.  I looked on line to many MSDS sheets from
different vendors and found only one that stated such storage
requirements for 10% NBF.  During this search I found all but one
company states that formalin is not flammable.  I brought this to the
Safety Officer.  He agrees that it is not flammable but that it IS
combustible.  Combustible=Flash point of 100F to 200F.  Of the dozen
sites I visited I found the following data concerning the Flash Point of
10% NBF: from NA / 200F / 122F to 185F.  The NFPA (National Fire
Protection Agency) guideline of no more than 1 gallon in a flammable
storage container and 1 gallon outside of a safety cabinet/container per
100 square feet is already quite limiting.  Using this guideline, we
have calculated acceptable volumes of the known flammables (Alcohols and
Xylenes) we can store.  Adding 10% NBF to the equation will have us
traveling to our bulk storage area constantly.  Does anyone out there
store 10%NBF in flammable cans/cabinets?
Riesen, Rebecca
rebecca.rie...@nchmd.org
NCH Healthcare Systems
Direct 239-436-5000 x2188
Fax 239-436-6767


Visit our website at http://www.nchmd.org


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