RE: [Histonet] discarding old dry chemicals with no expiration date
They did not reference any CLIA regulation. It was just suggested that the dry chemicals were not very useful after 10 years. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 --- On Wed, 9/30/09, Podawiltz, Thomas tpodawi...@lrgh.org wrote: From: Podawiltz, Thomas tpodawi...@lrgh.org Subject: RE: [Histonet] discarding old dry chemicals with no expiration date To: Kelly Boyd kdboydhi...@yahoo.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 4:15 PM What CLIA regulation did they use for reference for that statement? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhi...@yahoo.com] Sent: Wednesday, September 30, 2009 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] discarding old dry chemicals with no expiration date Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] discarding old dry chemicals with no expiration date
Here's the reference I use for that problem. But if the salt loses its saltiness, how can it be made salty again? It is no longer good for anything, except to be thrown out and trampled by men. Mk 9:50(ish or so. :) Happy Friday -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, September 30, 2009 3:40 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] discarding old dry chemicals with no expiration date I always have a problem determining the expiration date of sodium chloride! Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 30, 2009 4:27 PM To: histonet@lists.utsouthwestern.edu; Kelly Boyd Subject: Re: [Histonet] discarding old dry chemicals with no expiration date I was going to comment about how important some people seem to feel to find something to add to the inspection report even when it is unsubstantiated, about how many of those chemicals are extracted from mines where they have existed for eons, how the only important thing is to make sure that those designated as anhydrous have to be kept that way in order to assure the quality of the solutions and that everything else is almost ridiculous, but I better don't because I may hurt some feelings! René J. --- On Wed, 9/30/09, Kelly Boyd kdboydhi...@yahoo.com wrote: From: Kelly Boyd kdboydhi...@yahoo.com Subject: [Histonet] discarding old dry chemicals with no expiration date To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 1:24 PM Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] [Fwd: [PPMA] NSH meeting] Barcoding
For those of you interested in barcoding, this might be a worthwhile talk. Yes, he is my boss so I should put in a plug for him. Victor Original Message Subject:[PPMA] NSH meeting Date: Wed, 30 Sep 2009 17:17:37 -0700 (Pacific Daylight Time) From: Rodney Schmidt schmi...@u.washington.edu Reply-To: A mutual assistance forum for PowerPath users p...@u.washington.edu To: PowerPath Mutual Assistance p...@u.washington.edu For those of you attending the NSH meeting this next week, I hope to see some of you there. I'll be giving a talk on Tuesday morning about the quality and efficiency benefits of barcoding in the AP lab. If you'd like to get together for dinner or meet some other time, please drop me an off-line email. Unfortunately, my stay in Birmingham is going to be fairly short -- from Monday afternoon until Tuesday afternoon. See you there! Rodney A. Schmidt, M.D., Ph.D. Professor of Pathology Director of Medical Informatics University of Washington, Seattle, WA (206) 598-6462 (206) 344-0532 (pager) (206) 598-3803 (fax) = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ___ PowerPath Mutual Assistance [PPMA] mailing list p...@u.washington.edu http://mailman2.u.washington.edu/mailman/listinfo/ppma -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decal for a seahorse
I need to decal a sea horse. Would rather not use hydrochloric acid. Does anyone have a method that works? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: discarding old dry chemicals with no expiration date
Kelly D. Boyd, BS, HTL (ASCP) at Harris Histology Services in Greenville NC describes having a CLIA inspector ordering that all dry chemicals older than 10 years be discarded. Allen Smith sums it up The problem is letting people with little or no knowledge of chemistry make chemical decisions. To which I would add: and a system in which inspectors from agencies accountable to no one (e.g. JCAHO) are allowed to require summary changes in laboratory procedures, with no appeal allowed. Thirty years ago most histology labs did almost all of their own preparations - today making a 1% solution of periodic acid is considered high-level manufacturing. Allen Smith goes on to observe that Indigo used by Bar Kochba's soldiers to dye the fringes of their prayer shawls in the second century has been found in caves in the Judean desert; it is still indigo. Was this indigo, or was it the indigo-like dyes obtained from shellfish that they used? This remains a lively subject of debate among Jewish scholars. Present day Jews do not dye the fringes (tzitzit) of their prayer shawls (tallit or tallis), thinking that the technique has been lost (a vociferous fringe group - you might say - is using shellfish-derived dyes and has a cool T-shirt - I want one). Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thomas Crowell is out of the office.
I will be out of the office starting 10/01/2009 and will not return until 10/06/2009. Please contact Humphrey Gardner at 617-871-3590 if you have any questions regarding clinical trial samples. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: discarding old dry chemicals with no expiration date
The stuff found in the Judean caves is kal ilan, indigo derived from Indigofera tinctoria. It is chemically identical to tekhelet, indigo derived from Murex trunculus by photochemical debromination of Tyrian purple. Although some second and third century Jews, knowingly or unknowingly, used kal ilan instead of tekhelet, Rabbinic opinion has generally held that only tekhelet is kosher for dyeing the fringes. However, the Talmud also remarks that, Only God can tell the difference between tekhelet and kal ilan. Modern spectrometry can detect traces of cellulose in kal ilan, which are not present in tekhelet. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, October 01, 2009 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: discarding old dry chemicals with no expiration date Kelly D. Boyd, BS, HTL (ASCP) at Harris Histology Services in Greenville NC describes having a CLIA inspector ordering that all dry chemicals older than 10 years be discarded. Allen Smith sums it up The problem is letting people with little or no knowledge of chemistry make chemical decisions. To which I would add: and a system in which inspectors from agencies accountable to no one (e.g. JCAHO) are allowed to require summary changes in laboratory procedures, with no appeal allowed. Thirty years ago most histology labs did almost all of their own preparations - today making a 1% solution of periodic acid is considered high-level manufacturing. Allen Smith goes on to observe that Indigo used by Bar Kochba's soldiers to dye the fringes of their prayer shawls in the second century has been found in caves in the Judean desert; it is still indigo. Was this indigo, or was it the indigo-like dyes obtained from shellfish that they used? This remains a lively subject of debate among Jewish scholars. Present day Jews do not dye the fringes (tzitzit) of their prayer shawls (tallit or tallis), thinking that the technique has been lost (a vociferous fringe group - you might say - is using shellfish-derived dyes and has a cool T-shirt - I want one). Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LEAN at NSH
For any of you that are attending NSH in Birmingham and interested in the practical applications of LEAN, I will, along with a professional work flow analyst, be giving a workshop (WS # 8) on Saturday morning. The design is to give a basic overview of LEAN, demonstrate how the principles of LEAN were used in designing a new Pathology lab, and an exercise where the attendees will have the opportunity to design their own unique Path Lab using LEAN principles. It will be a great way to see how this catch phrase everyone is using these days can be put in to practice. Hope to see you there. Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Is anyone familiar with Granzyme B antibody?
I'm trying to find out some information on Granzyme B antibody for immunostaining of FFPE mouse tissue. Does anyone have any vendors they could recommend and/or protocols? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] marilyn weiss will be out of the office as of 5 a.m.10/1/09
I will be out of the office starting 10/01/2009 and will not return until 10/05/2009. I will respond to your message when I return.In my absence please ask for Mary Campbell or Laurie at 619-528-6801 if this is urgent they can contact me or give you my cell phone number. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura TDE 30
Could you please explain what the problems are. I have had excellent results with this system On 10/1/09, Isabel Cristina Soares Brandao isabel.bran...@sarah.br wrote: Hi, Does anyone out there have experience with the decalcifier System from Sakura? We have just recived the equipment but we could'nt get the results that they describe in the brochure. Isabel C.S. Brandão Associação das Pioneiras Sociais Patologia Cirúrgica - Brasília - Brasil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Is anyone familiar with Granzyme B antibody?
Wow, is it my day for familiar Abs or what?! We've used Abcam's Granzyme B (cat #ab4059) for both our mouse and human FFPE work. It works quite well, it's a RbPAb at 1/50. Let me know if you'd like me to forward the paper we used it on. Katy Message: 9 Date: Thu, 1 Oct 2009 09:35:24 -0700 From: Jennifer Campbell jcampb...@vdxpathology.com Subject: [Histonet] Is anyone familiar with Granzyme B antibody? To: histonet@lists.utsouthwestern.edu Message-ID: 5658cbdb9eae6545abe50d2563d81bf82ac...@vdxserver01.vdxpathology.local Content-Type: text/plain; charset=us-ascii I'm trying to find out some information on Granzyme B antibody for immunostaining of FFPE mouse tissue. Does anyone have any vendors they could recommend and/or protocols? Thanks, Jennifer -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 71, Issue 2 *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOP's reference
Hello everyone, I've been assigned recently to prepare the SOP's for the histology laboratory for the accreditation of my institution that englobes 4 major hospitals, that will take place next september (2010). As you well know it's a very demanding and time consuming task, so I'm asking your help, once again. If anyone will be able to send me the best sites where I can find the informations about different stains , like purpose , objectives etc , in order to facilitate the production of all these SOP's . Specially that I'm doing it only part time. Any info or references would be greatly appreciated. Thanks in advance Carmy _ Nous sommes vos photos. Partagez-nous dès maintenant avec Windows Live Photos. http://go.microsoft.com/?linkid=9666051___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] SOP's reference
Freda Carson; Histotechnology A Self Instruction Text. Sheehan Hrapchak: Theory and practice of Histotechnology Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net carmen loiselle carmen_loise...@hotmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 10/01/2009 02:24 PM To pathology education histo...@pathology.swmed.edu cc Subject [Histonet] SOP's reference Hello everyone, I've been assigned recently to prepare the SOP's for the histology laboratory for the accreditation of my institution that englobes 4 major hospitals, that will take place next september (2010). As you well know it's a very demanding and time consuming task, so I'm asking your help, once again. If anyone will be able to send me the best sites where I can find the informations about different stains , like purpose , objectives etc , in order to facilitate the production of all these SOP's . Specially that I'm doing it only part time. Any info or references would be greatly appreciated. Thanks in advance Carmy _ Nous sommes vos photos. Partagez-nous dès maintenant avec Windows Live Photos. http://go.microsoft.com/?linkid=9666051___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] paraffin sectioning static
Dryer sheets work well too - wipe the microtome down - and your clothes and hands.. Joyce -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, October 01, 2009 15:21 To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] paraffin sectioning static The things we've tried Emily are: rubbing a gauze soaked with Static Gard on and around the microtome, brushing the microtome with a Static Master brush, and/or laying down wet gauze around your blade holder. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, October 01, 2009 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin sectioning static Any tips on getting rid of static when paraffin sectioning on a microtome? It's so insanely bad, the block is picking up the ribbon as it goes back up every third section. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Is anyone familiar with Granzyme B antibody?
Unfortunately I am trying to get out of the lab and go home to pack for the NSH, so I do not have the catalog number, but eBioscinece has a good biotin anti mouse Granzyme B cat#13-8822 that works well on mouse tissue. They also have a biotin anti mouse F4/80. Good luck Dusko From: Jennifer Campbell jcampb...@vdxpathology.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 1, 2009 9:35:24 AM Subject: [Histonet] Is anyone familiar with Granzyme B antibody? I'm trying to find out some information on Granzyme B antibody for immunostaining of FFPE mouse tissue. Does anyone have any vendors they could recommend and/or protocols? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
