Re: [Histonet] Sakura TDE 30
OK, Firstly, what you do depends on what samples you are dealing with. We decalcify smallish samples of bone about 1x1cm - this can either compact or trabecular bone. Here are some of the things I learned.. 1. Do not overload the chamber.place samples between the electrodes, and rather do fewer samples - decal can be very quick if used right. 2. Do not use metal lids on casettes if using them (or any metal clips, parts etc) 3. If the samples aer heavily mineralised, ie huge pieces of compact bone, change the fluid when you see that the fizzing around the electrodes has slowed 4. Rather swich off the unit overnight (with the samples still in) if you are doing a long decal - then swich on in the day, so that you can monitor progress. 5. Not all sam[les will decalcify at the same rate - check them all individually at 4 - 5hr, or in the morning of the next day 5. When decal is finished - rinse samples thoroughly in running tap water for at LEAST and hour before processing 6. I have decalcified a slice of pig femur about 0.5 cm thick in about 5hrs hope this helps On Thu, Oct 1, 2009 at 7:48 PM, Isabel Cristina Soares Brandao isabel.bran...@sarah.br wrote: The time expended in the process was too long and the tissues had artifacts when examined on the microscope. We did exactly the same procedure described in the Sakura brochure. Do you do something different? How do you verify the process? How long does it take to get the samples decalcified? Thanks for your help! -- De: histonet-boun...@lists.utsouthwestern.edu[ SMTP:histonet-boun...@lists.utsouthwestern.edusmtp%3ahistonet-boun...@lists.utsouthwestern.edu] em nome de louise renton[SMTP:louise.ren...@gmail.comsmtp%3alouise.ren...@gmail.com ] Enviada: quinta-feira, 1 de outubro de 2009 14:35 Para: Histonet@lists.utsouthwestern.edu Assunto: Re: [Histonet] Sakura TDE 30 Could you please explain what the problems are. I have had excellent results with this system On 10/1/09, Isabel Cristina Soares Brandao isabel.bran...@sarah.br wrote: Hi, Does anyone out there have experience with the decalcifier System from Sakura? We have just recived the equipment but we could'nt get the results that they describe in the brochure. Isabel C.S. Brandão Associação das Pioneiras Sociais Patologia Cirúrgica - Brasília - Brasil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Basment membranes
Does anyone know any stains Specific for basement membrane? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Basement membranes
Periodic Acid-Methenamine Silver Procedure for Basement Membranes. Basement membranes will stain black with the Ag reaction. PAS will also work with the basement membrane showing PAS positive. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net William Lafayette Mundey wmo...@verizon.net Sent by: histonet-boun...@lists.utsouthwestern.edu 10/02/2009 07:36 AM Please respond to wmo...@verizon.net To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Basment membranes Does anyone know any stains Specific for basement membrane? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Amoeba Control
I am in need of an Amoeba control. Does anyone know of a vendor that would have these in stock, or other suggestions for finding one? Thank you very much. Gloria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Basement membranes
AKA Jones Method. Sigma sells a kit or you can make it all up yourself. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dkb...@chs.net Sent: Friday, October 02, 2009 6:51 AM To: wmo...@verizon.net Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Basement membranes Periodic Acid-Methenamine Silver Procedure for Basement Membranes. Basement membranes will stain black with the Ag reaction. PAS will also work with the basement membrane showing PAS positive. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net William Lafayette Mundey wmo...@verizon.net Sent by: histonet-boun...@lists.utsouthwestern.edu 10/02/2009 07:36 AM Please respond to wmo...@verizon.net To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Basment membranes Does anyone know any stains Specific for basement membrane? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Basement membranes
No, just in sections. René J. --- On Fri, 10/2/09, William Lafayette Mundey wmo...@verizon.net wrote: From: William Lafayette Mundey wmo...@verizon.net Subject: RE: [Histonet] Basement membranes To: dkb...@chs.net Cc: histonet@lists.utsouthwestern.edu Date: Friday, October 2, 2009, 9:22 AM Can the Periodic Acid-Methenamine Silver Procedure for Basement Membranes or the Jones be done in block? From: dkb...@chs.net [mailto:dkb...@chs.net] Sent: Friday, October 02, 2009 7:51 AM To: wmo...@verizon.net Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Basement membranes Periodic Acid-Methenamine Silver Procedure for Basement Membranes. Basement membranes will stain black with the Ag reaction. PAS will also work with the basement membrane showing PAS positive. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net William Lafayette Mundey wmo...@verizon.net Sent by: histonet-boun...@lists.utsouthwestern.edu 10/02/2009 07:36 AM Please respond to wmo...@verizon.net To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Basment membranes Does anyone know any stains Specific for basement membrane? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening-Cytopathology Fellow
Allied Search Partners is now accepting resumes for one of our client's in Phoenix, AZ. We are accepting resumes for a *Cytopathology Fellow.* Please submit your resume for prescreening purposes to aly...@alliedsearchpartners.com *All inquiries are always kept confidential* Once resume is submitted one of our recruiters will follow up with you for a phone screen. No resume will be submitted to our client until we speak to you. Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. Job Description: Cytopathology Fellowship Requirements: Board certification Fine needle aspiration experiences a plus -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eager's Method
Fellow Histonetters- I could use some help. I have a request to use a stain for sympathetic nerve fibers called Eager's that can be combined with Luxol Fast Blue / Crsyl Echt Violet. I know many of you would recommend GFAP or S100, and you would be absolutely correct. Unfortunately, that is not on the table here. I thank in advance any of you who respond with the reference I need. Joe Saby, BA HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: IHC POSITION WANTED
- Forwarded Message From: Isaac O o.isaa...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, September 23, 2009 6:55:49 PM Subject: IHC POSITION WANTED Hi, I am looking for a new IHC position. I am HTL(ASCP) certified. I am open to relocation. Isaac. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Does anyone have a procedure for the new Telepathology and digital imaging questions in the CAP questionnaire.
Does anyone have a procedure for the new Telepathology and digital imaging questions in the CAP questionnaire? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used equipment dealers
Hello, My name is Laura I recently visited http://lists.utsouthwestern.edu/pipermail/histonet/2009-June/045270.html page and I will like to suggest a useful resource to be added on your site. Title: CoreIndex.com URL: http://www.coreindex.com/ Description : A platform to find worldwide businesses and business partners among importers, exporters, traders and distributors. Thank you very much, Ms. Laura Keith ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histobath Frozen Sections
Hello, I am looking for a replacement for my Shandon Histo-bath. It is a snap freeze instrument that I have been using with Isopentane. Do you have something comparable to this or any suggestion? My Histobath finally gave up. Many thanks, Deborah K. Edwards, HTL/QIHC, (ASCP) Supervisor of Histology MEDVAMC Lab 113 2002 Holcombe Houston, Texas 77030 713-794-7259 (v.m.) e-mail: edwards.debor...@va.gov 713-794-7260 (histology lab) 713-794-7657 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histobath Frozen Sections
Dear Deborah, Yes we can help you on this, we manufacture the Clini-RF Rapid Freezer which goes down to -80 degs. C. Please see this model on www.brightinstruments.com Best Regards Alan Bright Sent from my BlackBerry® wireless device -Original Message- From: Edwards, Deborah K edwards.debor...@va.gov Date: Wed, 30 Sep 2009 15:04:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath Frozen Sections Hello, I am looking for a replacement for my Shandon Histo-bath. It is a snap freeze instrument that I have been using with Isopentane. Do you have something comparable to this or any suggestion? My Histobath finally gave up. Many thanks, Deborah K. Edwards, HTL/QIHC, (ASCP) Supervisor of Histology MEDVAMC Lab 113 2002 Holcombe Houston, Texas 77030 713-794-7259 (v.m.) e-mail: edwards.debor...@va.gov 713-794-7260 (histology lab) 713-794-7657 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Araceli Davis is out of the office will return 10-8-09
I will be out of the office starting 10/02/2009 and will not return until 10/09/2009. Any histology request contact histol...@wlgore.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
