[Histonet] time in paraffin and fried bloody specimen
Good Morning Histonetters- First question: Textbook says tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening. Can anyone define exposure to prolonged heat? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Bond/Novocastra Reagents
Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Bond/Novocastra Reagents
We get almost all their antibodies and ancillaries and have never had a problem! They are a great company and we love the Bond Max. Josie Britton HT Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 AM To: histo...@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Bond/Novocastra Reagents
We have never had a backorder problem- have 2 Bondmax's over 2 years. I love them. Jo Mauger Josie Britton jcbrit...@cheshire-med.com 10/05/09 11:29 AM We get almost all their antibodies and ancillaries and have never had a problem! They are a great company and we love the Bond Max. Josie Britton HT Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 AM To: histo...@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Bond/Novocastra Reagents
We've had no problems with either Bond or Novacastra orders. Joyce -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 To: histo...@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond/Novocastra Reagents
Almost always receive on time. I can think of only one instance in a year and a half where anything was on back order (it was an antibody). Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Paula Lucas plu...@biopath.org 10/5/2009 12:20 PM Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] time in paraffin and fried bloody specimen
After you have developed a processing protocol and obtained good infiltration after a certain time (hours) in paraffin, any and all the time above that period of adequate infiltration = exposure to prolonged heat. Some histotechs even don't fill the holding chamber in the embedding center, a practice I do not think is adequate. To your second question, just place them in NBF and when fixed filter and wrap them yourself while cassetting, do not wrap them before being fixed. René J. --- On Mon, 10/5/09, Nancy Schmitt nancy_schm...@pa-ucl.com wrote: From: Nancy Schmitt nancy_schm...@pa-ucl.com Subject: [Histonet] time in paraffin and fried bloody specimen To: Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Date: Monday, October 5, 2009, 11:06 AM Good Morning Histonetters- First question: Textbook says tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening. Can anyone define exposure to prolonged heat? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] time in paraffin and fried bloody specimen
Nancy, Tissue should be processed @ between 60-62 degrees centigrade. We have three paraffin baths. The 1st bath is set for 45 mins, the 2cd and 3rd are for 1 hour each. This is for large specimens. Small specimens are for 30 mins. the first two baths and 45 mins for the last. It is very true that too much time in paraffin causes hard tissue. Remember the whole time the tissue is setting in paraffin it is being exposed to heat. Your second question: Have the specimen transferred from the lens paper it arrived in and put on a new piece which has been moistened with formalin. Sometimes in surgery the lens paper is wet with saline. If it is a scant amount process with your Endoscopic biopsies. Too long in your alcohols will over dehydrate the specimen. Hope this helps. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net Nancy Schmitt nancy_schm...@pa-ucl.com Sent by: histonet-boun...@lists.utsouthwestern.edu 10/05/2009 11:07 AM To Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu cc Subject [Histonet] time in paraffin and fried bloody specimen Good Morning Histonetters- First question: Textbook says tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening. Can anyone define exposure to prolonged heat? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Brain Frozen sections
There is nothing you can do to make brain frozen sections acceptable. Your Pathologists need to learn how to read smears, or just accept being wrong 50% of the time. An educated guess based on the imaging is more accurate than frozen sections on intra-axial primary brain tumors. Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, October 05, 2009 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 71, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Desperately need frozen kidney (Scott Gill) 2. Brain Frozen sections (Anne van Binsbergen) 3. RE: Histobath Frozen Sections (Galbraith, Joe) 4. time in paraffin and fried bloody specimen (Nancy Schmitt) 5. Leica Bond/Novocastra Reagents (Paula Lucas) 6. RE: Leica Bond/Novocastra Reagents (Josie Britton) 7. RE: Leica Bond/Novocastra Reagents (Joanne Mauger) 8. Re: Leica Bond/Novocastra Reagents (Greg Dobbin) 9. RE: Leica Bond/Novocastra Reagents (Weems, Joyce) 10. Re: time in paraffin and fried bloody specimen (Rene J Buesa) 11. Re: time in paraffin and fried bloody specimen (dkb...@chs.net) -- Message: 1 Date: Sun, 4 Oct 2009 20:39:00 -0700 (PDT) From: Scott Gill histot...@yahoo.com Subject: [Histonet] Desperately need frozen kidney To: histonet@lists.utsouthwestern.edu Message-ID: 573555.19370...@web58303.mail.re3.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hello, ? My research project is in desperate need of a couple fresh frozen pieces of kidney positive for C3 and/or C4.? (lupus?)? I will gladly pay any costs to get the tissue sent.? (boxes, dry ice, postage, etc.) ? Thanks in advance for your time Scott histot...@yahoo.com.? -- Message: 2 Date: Mon, 5 Oct 2009 08:38:06 +0400 From: Anne van Binsbergen anni...@gmail.com Subject: [Histonet] Brain Frozen sections To: histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: f8332fbe0910042138r2ecd55b7ve63e503ec6afc...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters we are having INTERMITTENT problems with brain frozens all conditions are the same ie. same tech, same cryostat, same freezing technique - sometimes there is freezing artefact in the sections and sometimes the sections look like they were cut from an FFPE block!! unfortunately not all our pathologists are confident to make an IOC diagnosis on a smears so we are forced to resort to freezing. my tech is very experieince and says she can almost predict when looking at the tissue, that there will be artefact on the sections. pathologists are insisting on the FZ but complaining bitterly at the freezing artefact. I suspect the surgeon may be 'flooding' the tissue with saline but unless we breathe down their necks we cannot possibly know what goes on in the OR! All other frozens are fine - its just the very small neuro ones we are having a problem with and it is driving us to distraction - well, actually the Pathologists are doing that!! we are using cryospray, gently sprayed onto the tissue, which is covered with a small drop of fresh OCT, on top of an already frozen button of OCT which is kept in the cryostat there is no problem with other frozen sections, on other tissues, prepared in exactly the same way any and all observations/recommendations/suggestions will be greatfully accepted TIA -- Anne van Binsbergen (Hope) Abu Dhabi UAE -- Message: 3 Date: Mon, 5 Oct 2009 09:16:41 -0500 From: Galbraith, Joe joseph-galbra...@uiowa.edu Subject: RE: [Histonet] Histobath Frozen Sections To: mwh...@mcleodhealth.org, histonet@lists.utsouthwestern.edu Message-ID: aeecb16e79b57342806128d46af1687905c91...@hc-mail10.healthcare.uiowa.edu Content-Type: text/plain; charset=us-ascii Melanie: We are acquiring a SnapFrost 2 unit from Alphelys. They offer two models - one at -80 and one at -100. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mwh...@mcleodhealth.org Sent: Wednesday, September 23, 2009 2:21 PM To:
[Histonet] Paraffin sectioning mouse embryos
Dear Histonetters, I hope you can help me. I'm working with mouse embryos. Now I have some samples (~ E.10.5), which have already gone through ISH. I tried to make some paraffin sectionings from them, but most of the sectionings were compressed anddid't look very nice. I tried the processing with different times and the slices in different thicknesses. Younger embryos w/o ISH get very hard and break easily. Hopefully one of you has an idea ;-) Thank you very much, Sandra ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
