[Histonet] Question on certification requirements for grossing in the state of Michigan
Hi Histonetters, I have a quick question and am hoping someone can either answer it or send me to the right place to get the answer. Is an ASCP HTL certification required in Michigan in order to be able to gross or is an HT allowed to gross as well? Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: rel...@earthlink.net mailto:rel...@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re:FS stainer
Hello, Does anyone know of any FS stainer other then the Shandon Linistat Linear Stainer? Thanks Sue Sudbury Regional Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Knifemaker LKB supply
I need help. It is urgent to replace in my lab off electron microscopy, the cuting wheel for model 7801b lkb knifemaker. I have not found it here, and I'm looking in other hemisphere or country. Thanks all! Ht Agustin V Chertcoff Electron Microscopy Service National Institute off Microbiology C G Malbran Buenos Aires Argentina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Immunohistochemistry fixation
Katherine Walters, Histology Director, Central Microscopy Research Facilities, University of Iowa asks: Has anyone has any experience using immunohistochemistry with the following fixation? Polyethylene glycol (PEG) solution: 25% glycol, 10% ethanol (95%), 10% formaldehyde (37%), and 55% distilled water. Specifically if there are any enzymatic reactions that I should avoid/block for/etc. - Thanks for any hints-I am pretty much wed to this fixative. You may be wed to it, but you may be being two-timed if you are. Polyethylene glycol (trade name Carbowax, from Dow Chemical) is not a single substance, but comes in a wide range of molecular weights with physical state anywhere from a watery liquid to a paraffin-like solid. See the Wikipedia article. If you're using a commercial product containing PEG, the manufacturer may know the answer. If you're compounding it yourself, you may just have to try the IHC's and see. Any publications should cite the molecular weight (or Carbowax number) of the PEG in the fixative. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 72, Issue 17
Sue, TBS had a frozen section stainer on display at NSH this year. I don't have the information right here in front of me, but if you are interested I will forward it to you. Joanne Clark, HT(ASCP)MLT(CSMLS) Path Consultants of New Mexico Roswell, NM Message: 4 Date: Mon, 16 Nov 2009 11:49:45 -0500 From: Seguin, Suzanne sseg...@hrsrh.on.ca Subject: [Histonet] Re:FS stainer To: histonet@lists.utsouthwestern.edu Message-ID: b5480a2ca1e16643800e9d572fa21ff8035b4...@resexcvs1.res.neo.local Content-Type: text/plain; charset=us-ascii Hello, Does anyone know of any FS stainer other then the Shandon Linistat Linear Stainer? Thanks Sue Sudbury Regional Hospital -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat decontamination...
From what I understand if you require that an individual wear a N95 respirator when they are sectioning frozen sections then you are also required to have that respirator fit tested yearly. We are a research lab that does quite a bit of work on TB samples. We do not section TB infected samples on a cryostat. All samples that we receive have been fixed in 10% NBF for several days and then transferred to 70% alcohol. When I was in a clinical lab, we would not section frozen sections of lung samples if they wanted to rule out TB, the pathologist would recommend to process to paraffin first. In fact since the samples are generated in a biosafety level 2 or 3 facility, they can not leave that facility unless the samples have been fixed and rendered non infectious. Frozen sections would need to be prepared within that biosafety facility with all of the appropriate PPE's in place. The University that we work with has tested these samples via culture after their fixation and alcohol procedure. We do however offer the N95 respirator to the techs and they can wear it if they want to, when they are grossing, embedding or sectioning these samples. But since it is voluntary we do not have to fit test, we have a specific procedure that covers this. This protocol has been developed with the help of our local OSHA rep. The other thing is we use a special vacumme with a hepa filter to vacume up the paraffin trimmings. OSHA has a program for small businesses and will work with them to make sure that they are within compliance, they have been very helpful to us here. Just go to the OSHA website. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, November 16, 2009 12:17 PM To: histo...@pathology.swmed.edu Subject: [Histonet] Cryostat decontamination... Hello all, We have always used absolute alcohol to decontaminate our cryostat and wondered what others use. This has been brought up by an article in the September issue of CAP Today that talks about biosafety when doing frozens (specifically talks about TB). As a related question, how many institutions require the wearing on an N95 respirator when doing frozens? The article states that diluted alcohol is more effective because the presence of water causes proteins to denature more quickly. I just did the decontamination of our cryostat last weekend and used 70% alcohol as the article suggested. The alcohol evaporated and I was left with beads/drops of water that I had to then dry by hand. I guess the alternative would be is just to go over it again with aboslute after usiing 70%. The article goes on to recommend that personnel should wear N95 masks that are fit tested on a yearly basis. Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] B5 alternate
Essentially it's a zinc formalin. Drew Sent from my iPhone On Nov 16, 2009, at 4:53 PM, John Kiernan jkier...@uwo.ca wrote: What is this B plus fixative? Is it a trade-secret brew? John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Anne van Binsbergen anni...@gmail.com Date: Friday, November 13, 2009 10:01 Subject: Re: [Histonet] B5 alternate To: Drew Meyer 41dm...@gmail.com Cc: Knutson, Deanne dknut...@primecare.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu B plus is the one - we use it as stated and it works like a charm immunos are good too - we are proudly mercury free Annie 2009/11/13 Drew Meyer 41dm...@gmail.com In my lab, we're using B-Plus Fixative by BBC Biochemical. I know many other labs that are also using it as their B5 alternative. The biggest pro, of course, is that it's mercury free and not hazardous. Also, it's not as sensitive to over-fixation like B5 is. The guideline I use is 4 hours minimum fixation for Bone Marrows and Lymphoid tissue. After that, routine formalin processing is all you need to do. You can, however, leave the specimen in B- Plus for up to 48 hours without adversely effecting the tissue sample. Hope this help. Drew Meyer On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne dknut...@primecare.org wrote: Am looking for advice on what alternate fixative to use to replace B5. What do the majority of you histonetters use? Does it work well with immunos? The pros and cons? Thank you for your recommendations. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknut...@primecare.org mailto:dknut...@primecare.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] test
This is a test. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BrdU EM
Just wondering anyone has an idea to solve this problem? __ Yes, I have tried with all kinds of concentrations of HCl and different times of incubation as well. The BrdU labelling works even with 10 minutes of HCl incubation (this is not very consistent, though) but the ultrastructure is 'dead' by that time. Has anyone got any experience with breaking DNA strands using UV light? What's the wavelength that could be used and how long does the tissue need to be exposed? My first try was our fluorescent microscope (at 360-370 nm), exposure time 1 hour, but it doesn't seem to do the job . Szilvi Mezey Subject:RE: [Histonet] BrdU and EM? Date sent: Thu, 18 Sep 2003 09:40:23 +0100 From: Edwards, R.E. To: Mezey Szilvia Have you tried cutting down the HCl time to a minimum? Richard Edwards MRC TOX UNITU.K... -Original Message- From: Mezey Szilvia [mailto:me...@ana.sote.hu] Sent: 17 September 2003 12:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BrdU and EM? Hello All, I'm trying to do BrdU staining (in paraformaldehyde fixed, free- floating sections) and preserve the ultrastructure of the tissue for EM at the same time. HCl works fine for denaturing DNA for BrdU- ICC but doesn't leave much of the tissue for EM. DNase would be more EM-friendly but doesn't penetrate the tissue enough. Does anybody know a method that could provide a fair enough compromise between BrdU and EM? Maybe by increasing the penetration of DNase? Best regards to you all, Szilvi Szilvia Mezey PhD student Semmelweis University Dept. of Anatomy, Histology and Embryology Tuzolto u. 58. Budapest, 1094, Hungary T.: +36-12156920/3687 F.: +36-12155158 E-mail: me...@ana.sote.hu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Szilvia Mezey PhD student Semmelweis University Dept. of Anatomy, Histology and Embryology Tuzolto u. 58. Budapest, 1094, Hungary T.: +36-12156920/3687 F.: +36-12155158 E-mail: me...@ana.sote.hu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet 2009-11-17 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
