RE: [Histonet] Bachelor's Degrees
Tom, I agree with you about Nate qualifications. Unfortunately in NYS if you bring in outside work your tech MUST be licensed in NY. The copy of the license need to be displayed in the lab. I haven't found any way to hire employees without a license. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, December 05, 2009 3:23 PM To: Nathan Jentsch Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bachelor's Degrees One more reason to consider carefully before throwing support to state licensure where it does not exist. I feel sorry for you Nathan and I'd like to have someone explain the upside of licensing to you. It seems it's not about having a license (like a driver's license) to practice histology. I fear it's just more about fattening state coffers and adding another level of bureauracracy to things. If you are educated (as you are Nate) and if you are academically eligible to sit for the registry exam. And if you can satisfactorily pass the exam, what has state licensing got to do with it? Are you a better histologist in New York because you're licensed as opposed to your neighbors in PA, for example who aren't? I think not. Does licensing prove something that science degrees and registry certifications do not? Maybe I just don't get it. And I'm not trying to pick a fight here with the supporters of licensing. I just haven't heard a good convincing argument for it yet. I'm also quite certain that even though monetary compensation has improved somewhat, the last thing most Histologists need is another payment. The privilege to work in a certain state, which is paid for (by you) nothing more?! I suppose some kindly employers out there somewhere could pay for it...good luck with that. Here's an idea, let's say you're degreed and registry eligible and/or have passed your board exam(s) and are certified. How 'bout the state says you've met the qualification for licensing, here you go! Nate you are degreed and certified and in my book and in the book of the current state I live in - Oregon - and the states I've worked in - Wisconsin, Michigan and Minnesota - you are more than qualified to work. I for one would not hesitate in the least to consider a person such as yourself for employment. Again you are more than qualified, even though you are unlicensed. I guess I just don't understand how credentialing - degrees and certifications - aren't enough, but licensing is the magic ticket to better science/medicine/patient care/whatever. I'm sure some folks out there will bring on the firestorm, but again Nathan I feel sorry for you and I don't see the reasoning behind this. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Saturday, December 05, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bachelor's Degrees Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license. They won't even consider HT certification as sufficient. If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide handling for special stains
I don't see any point in leaving deparaffinized and rehydrated(!) slides any longer in water. The tissue might even fall off. What special stains are you goint to do? Hello all, I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rchar...@state.pa.usmailto:rchar...@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide handling for special stains
I wouldn't make that a habit. It eventually saturates the cells and then they fall off like the previous poster mentioned. Also, especially leaving them in water for a whole weekend you risk contamination. Not good practice. If you absolutely have to leave them in something after they have been deparaffinized I would suggest 95% alcohol. With that said, I think best practice would be to leave them paraffinized until you have time to do the stains. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Charles, Roger rchar...@state.pa.us Sent by: histonet-boun...@lists.utsouthwestern.edu 12/07/2009 07:19 AM To Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu cc Subject [Histonet] slide handling for special stains Hello all, I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rchar...@state.pa.usmailto:rchar...@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bachelor's Degrees
Cindy: And not being able to hire anybody without the license is absolutely great and why the license was created in the firs place, namely, to avoid having low paid people doing histology work in detriment to others more qualified. The salary competition posed by those not licensed is the cause for the low pay histotechs receive when compared with other professionals in the medical laboratory. That was exactly the point: to assure a just salary for histotechs. René J. --- On Mon, 12/7/09, Cynthia Pyse cp...@x-celllab.com wrote: From: Cynthia Pyse cp...@x-celllab.com Subject: RE: [Histonet] Bachelor's Degrees To: 'Thomas Jasper' tjas...@copc.net, 'Nathan Jentsch' njblademas...@gmail.com Cc: histonet@lists.utsouthwestern.edu Date: Monday, December 7, 2009, 8:12 AM Tom, I agree with you about Nate qualifications. Unfortunately in NYS if you bring in outside work your tech MUST be licensed in NY. The copy of the license need to be displayed in the lab. I haven't found any way to hire employees without a license. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, December 05, 2009 3:23 PM To: Nathan Jentsch Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bachelor's Degrees One more reason to consider carefully before throwing support to state licensure where it does not exist. I feel sorry for you Nathan and I'd like to have someone explain the upside of licensing to you. It seems it's not about having a license (like a driver's license) to practice histology. I fear it's just more about fattening state coffers and adding another level of bureauracracy to things. If you are educated (as you are Nate) and if you are academically eligible to sit for the registry exam. And if you can satisfactorily pass the exam, what has state licensing got to do with it? Are you a better histologist in New York because you're licensed as opposed to your neighbors in PA, for example who aren't? I think not. Does licensing prove something that science degrees and registry certifications do not? Maybe I just don't get it. And I'm not trying to pick a fight here with the supporters of licensing. I just haven't heard a good convincing argument for it yet. I'm also quite certain that even though monetary compensation has improved somewhat, the last thing most Histologists need is another payment. The privilege to work in a certain state, which is paid for (by you) nothing more?! I suppose some kindly employers out there somewhere could pay for it...good luck with that. Here's an idea, let's say you're degreed and registry eligible and/or have passed your board exam(s) and are certified. How 'bout the state says you've met the qualification for licensing, here you go! Nate you are degreed and certified and in my book and in the book of the current state I live in - Oregon - and the states I've worked in - Wisconsin, Michigan and Minnesota - you are more than qualified to work. I for one would not hesitate in the least to consider a person such as yourself for employment. Again you are more than qualified, even though you are unlicensed. I guess I just don't understand how credentialing - degrees and certifications - aren't enough, but licensing is the magic ticket to better science/medicine/patient care/whatever. I'm sure some folks out there will bring on the firestorm, but again Nathan I feel sorry for you and I don't see the reasoning behind this. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Saturday, December 05, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bachelor's Degrees Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license. They won't even consider HT certification as sufficient. If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet
Re: [Histonet] slide handling for special stains
No, it is not a good idea. In many occasions leaving the dewaxed sections in DW for prolonged periods of time can cause the section to peel off (not always, but sometimes). The idea is to dewax and do the special stains immediately after. René J. --- On Mon, 12/7/09, Charles, Roger rchar...@state.pa.us wrote: From: Charles, Roger rchar...@state.pa.us Subject: [Histonet] slide handling for special stains To: Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Date: Monday, December 7, 2009, 8:19 AM Hello all, I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rchar...@state.pa.usmailto:rchar...@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] opinion please
Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CM 3600
Looking for someone close to Pittsburgh, PA (or within a 300 miles) with a Leica CM3600 that I could come by and look at. Thanks, Terry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] opinion please
Amazing! How hot do you keep the room temp there? I would toss them, one false test on a patient is more valuable than all those lost reagents. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Kim Merriam kmerriam2...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 12/07/2009 08:51 AM To Histonet histonet@lists.utsouthwestern.edu cc Subject [Histonet] opinion please Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] opinion please
Perhaps yes or perhaps no, that is the problem. From now on you cannot know if something that did not work was because of the fridge malfunction. Advise? (Costly advise?): discard everything and get an alarm system for your fridge after you repair it (or get a new one).. René J. --- On Mon, 12/7/09, Kim Merriam kmerriam2...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] opinion please To: Histonet histonet@lists.utsouthwestern.edu Date: Monday, December 7, 2009, 9:51 AM Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] opinion please
I agree with Renee. It will proabaly be at least as costly to have to re-validate everything! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Rene J Buesa rjbu...@yahoo.com 12/7/2009 11:21 AM Perhaps yes or perhaps no, that is the problem. From now on you cannot know if something that did not work was because of the fridge malfunction. Advise? (Costly advise?): discard everything and get an alarm system for your fridge after you repair it (or get a new one).. René J. --- On Mon, 12/7/09, Kim Merriam kmerriam2...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] opinion please To: Histonet histonet@lists.utsouthwestern.edu Date: Monday, December 7, 2009, 9:51 AM Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: OT fuchsia
One of the researchers here summed it up nicely when he reported that most discoveries in the lab are not heralded with cries of Eureka!, but rather with hmmmnow that's interesting... Teri Johnson, HT(ASCP)QIHC Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Glypican 3
Anyone out there know were Glypican 3 antibody for IHC may be purchased from. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Overstaining - Mayers HE
You have to consider that the thicker the section the more intense the staining will be. If you are using now 1 minute and they are still too dark, reduce the staining time even further. Otherwise I don't see anything wrong with the rest of the protocol. René J. --- On Mon, 12/7/09, Josephine Garcia j...@u.washington.edu wrote: From: Josephine Garcia j...@u.washington.edu Subject: [Histonet] Overstaining - Mayers HE To: histonet@lists.utsouthwestern.edu Date: Monday, December 7, 2009, 11:42 AM Hi all, My (frozen-section, fixed) slides are coming out much too dark (overstained purple) and I'm not sure why. They are 15-20 micrometer slices of rat gastrocnemius muscle. Can someone please look over our current protocol and tell me what I'm doing wrong? Thanks! Here it is: 1. Perfuse animal with 4% paraformaldehyde fixative. 2. Soak in 0.4% paraformaldehyde 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) 4. Embed in OCT, Frozen sections (15-20 micrometers) 5. Let dry for 15-30 min 6. Stain as follows: - Distilled H2O (quick dip) - Mayer's Hematoxylin - 1min (originally we were dipping for 5-10 minutes. I slowly reduced the time to 2min, then 1min, then 30s... still overstained!) - Running lukewarm tap water - drain and continuously fill - 15min or until water runs clear - Distilled H2O (quick dip) - 80% EtOH - 1-2min - Eosin - 2 min - 95% EtOH I - 10sec - 95% EtOH II - 10sec - 100% EtOH I - 10sec - 100% EtOH II - 10sec - Xylene I - 2min - Xylene II - 2min 7. Coverslip and let dry overnight ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Coarse abrasive
From: Hannen, Valerie Sent: Friday, December 04, 2009 9:43 AM To: 'histo...@list.utsouthwestern.edu' Subject: Coarse abrasive Help!! My section chief just got word that Leica is no longer producing coarse abrasive. We only have steel blades in our department and we are SO dependent on the abrasives. She does not want to switch to disposable blades. Is there anyone out there in Histo-land who might know a solution to our dilema. Thanks in advance for your answers. Valerie Hannen,MLT(ASCP),HTL,SU(FL) Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32796 ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: Coarse abrasive
Hello Valerie, I believe you may have been given misinformation regarding the knife sharpening abrasives. You can order through Leica Microsystems using catalog number 14041819700 for a single bottle or 14937000 for a case of 6 bottles. Please feel free to contact me if you have any questions. Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! Hannen, Valerie valerie.han...@p arrishmed.com To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] FW: Coarse abrasive 12/07/2009 12:01 PM From: Hannen, Valerie Sent: Friday, December 04, 2009 9:43 AM To: 'histo...@list.utsouthwestern.edu' Subject: Coarse abrasive Help!! My section chief just got word that Leica is no longer producing coarse abrasive. We only have steel blades in our department and we are SO dependent on the abrasives. She does not want to switch to disposable blades. Is there anyone out there in Histo-land who might know a solution to our dilema. Thanks in advance for your answers. Valerie Hannen,MLT(ASCP),HTL,SU(FL) Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32796 ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Overstaining - Mayers HE
There doesn't appear to be anything wrong with the staining schedule. Mayer published more than one alum hematoxylin and one of them is a strong regressive type, and would produce the staining you see. Is it possible that has been used instead of his progressive one? If it has, make the following diluent. Potassium or ammonium alum 50 g Citric acid 1 g or glacial acetic acid 20 mL Distilled water 1L Then dilute the strong alum hematoxylin 1 part with 3 parts diluent. Or, Immediately before the 80% ethanol prior to the eosin, place into a solution of 0.5% acid ethanol for a few seconds. Wash well to blue, and carry on. If that doesn't correct it, then make up a fresh batch of Mayer's hemalum. Bryan Llewellyn - Original Message - From: Josephine Garcia j...@u.washington.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, December 07, 2009 8:42 AM Subject: [Histonet] Overstaining - Mayers HE Hi all, My (frozen-section, fixed) slides are coming out much too dark (overstained purple) and I'm not sure why. They are 15-20 micrometer slices of rat gastrocnemius muscle. Can someone please look over our current protocol and tell me what I'm doing wrong? Thanks! Here it is: 1. Perfuse animal with 4% paraformaldehyde fixative. 2. Soak in 0.4% paraformaldehyde 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) 4. Embed in OCT, Frozen sections (15-20 micrometers) 5. Let dry for 15-30 min 6. Stain as follows: - Distilled H2O (quick dip) - Mayer's Hematoxylin - 1min (originally we were dipping for 5-10 minutes. I slowly reduced the time to 2min, then 1min, then 30s... still overstained!) - Running lukewarm tap water - drain and continuously fill - 15min or until water runs clear - Distilled H2O (quick dip) - 80% EtOH - 1-2min - Eosin - 2 min - 95% EtOH I - 10sec - 95% EtOH II - 10sec - 100% EtOH I - 10sec - 100% EtOH II - 10sec - Xylene I - 2min - Xylene II - 2min 7. Coverslip and let dry overnight ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FL Supervisor License
Marie, If the person is licensed as a technologist with no ASCP certification but at least 5 years of experience, he/she qualifies under rule 64B3-5.002(C)(3). See below. 64B3-5.002 Supervisor. Qualifications and Responsibilities. (1) Qualification. Degrees or semester hours of academic credit required in this section shall be obtained at a regionally accredited college or university or by foreign education equated pursuant to subsection 64B3-6.002(6), F.A.C. In order to be licensed as a supervisor, an applicant shall be licensed or meet the requirements for licensure as a technologist, have a Board approved 2-hour course relating to the prevention of medical errors, which shall include root-cause analysis, error reduction and prevention, patient safety, complete an educational course acceptable to the Department on human immunodeficiency virus and acquired immune deficiency syndrome, and one of the following: (c) Histology Route 1 Five years of pertinent clinical laboratory experience in histology and 25 hours of Board-approved continuing education in supervision and administration within the previous 5 years and HTL (ASCP) Route 2 Five years of pertinent clinical laboratory experience post-certification and 48 hours of Board-approved continuing education in supervision and administration within the previous 5 years and HT (ASCP) Route 3 Five years of pertinent clinical laboratory experience, and 48 hours of Board-approved continuing education in supervision and administration within the previous 5 years, and licensure as a technologist in the specialty of histology Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville 655 W 8th Street Jacksonville, FL 32209 Tel: 904-244-6149 E-mail: jerry.santi...@jax.ufl.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histospr...@aol.com Sent: Wednesday, December 02, 2009 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FL Supervisor License This may have been covered already, please accept my apologies for a repeat question. I was curious if anyone knows if you can upgrade a technologist (FL state license only, grandfathered in so NO ASCP) license WITHOUT a 4 year degree? The work experience is 10+ years but this person does NOT have a 2 or 4 year degree of any kind. I know the laws constantly change, was just curious about the current ones, I am not familiar with ASCP guidelines for FL. Thank you in advance. Marie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] opinion please
Kim, Test a random sampling (10%) of the antibodies and see if they work. If so, then they are most likely fine. Document the test. Antibodies are very robust and the warmup probably won't damage many, if any, of them. BTW, When I worked for an antibody manufacturer I did a test once as part of a stability problem investigation and left six different antibodies in a 37C oven for up to 4 months. We tested a sample of each weekly. All worked fine and some even worked better even after 4 months! The issue I would be concerned about is bacterial growth in any of them. Keep an eye on that. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, December 07, 2009 6:51 AM To: Histonet Subject: [Histonet] opinion please Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] plasminogen activator inhibitor 1 (PAI 1) antibody
Has anyone had any experience with this antibody? There are many companies selling them and I'm not sure which would be best for my paraffin-embedded human tissue? Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] opinion please
I agree with Tim. This is a very common practice for antibody manufacturer's. Also, manufacturer's will revalidate antibodies when they are close to their exploration date and if that antibody has the same sensitivity. They will extend the shelf-life date. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote: Kim, Test a random sampling (10%) of the antibodies and see if they work. If so, then they are most likely fine. Document the test. Antibodies are very robust and the warmup probably won't damage many, if any, of them. BTW, When I worked for an antibody manufacturer I did a test once as part of a stability problem investigation and left six different antibodies in a 37C oven for up to 4 months. We tested a sample of each weekly. All worked fine and some even worked better even after 4 months! The issue I would be concerned about is bacterial growth in any of them. Keep an eye on that. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, December 07, 2009 6:51 AM To: Histonet Subject: [Histonet] opinion please Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] opinion please
Sorry I made a type O I meant to say Also, manufacturer's will revalidate antibodies when they are close to their expiration date and if that antibody has the same sensitivity. They will extend the shelf-life date. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Dec 7, 2009, at 5:02 PM, Akemi Allison wrote: I agree with Tim. This is a very common practice for antibody manufacturer's. Also, manufacturer's will revalidate antibodies when they are close to their exploration date and if that antibody has the same sensitivity. They will extend the shelf-life date. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote: Kim, Test a random sampling (10%) of the antibodies and see if they work. If so, then they are most likely fine. Document the test. Antibodies are very robust and the warmup probably won't damage many, if any, of them. BTW, When I worked for an antibody manufacturer I did a test once as part of a stability problem investigation and left six different antibodies in a 37C oven for up to 4 months. We tested a sample of each weekly. All worked fine and some even worked better even after 4 months! The issue I would be concerned about is bacterial growth in any of them. Keep an eye on that. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, December 07, 2009 6:51 AM To: Histonet Subject: [Histonet] opinion please Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mircom 505 E Cryostat 0000
Hi, We have a microm 505 E cryostat in the lab, the microtome part of which a repairer came to clean. Since the return of the microtome to the cryostat, when we turn it on we no longer get any of the error messages specified in the manual (such as those that come up when the cryostat has been off for a while and is not cool), but rather the displays show . The blank reset button does not work and nor do any of the other buttons. I can find no reference to this display in our manual. Does anyone know what means or how it can be fixed? Many Thanks, Karen. PhD Candidate School of Biological Sciences University of Queensland Goddard Building (8), Rm 363a ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] opinion please
Hello I have the same experience. When I worked for a company and right after 9/11, we shipped antibodies to Israel via Post office at ambient temp. This package came back after 6-8 weeks or may be longer. We do not know how they were stored at post office. We tested the antibodies and could not find differences in the activity using IHC. Bader On Mon, Dec 7, 2009 at 4:02 PM, Akemi Allison akemiat3...@yahoo.com wrote: I agree with Tim. This is a very common practice for antibody manufacturer's. Also, manufacturer's will revalidate antibodies when they are close to their exploration date and if that antibody has the same sensitivity. They will extend the shelf-life date. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote: Kim, Test a random sampling (10%) of the antibodies and see if they work. If so, then they are most likely fine. Document the test. Antibodies are very robust and the warmup probably won't damage many, if any, of them. BTW, When I worked for an antibody manufacturer I did a test once as part of a stability problem investigation and left six different antibodies in a 37C oven for up to 4 months. We tested a sample of each weekly. All worked fine and some even worked better even after 4 months! The issue I would be concerned about is bacterial growth in any of them. Keep an eye on that. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, December 07, 2009 6:51 AM To: Histonet Subject: [Histonet] opinion please Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience corp. Phone: + 425 367 4601 Phone: + 425 514 3761 Fax: + 425 367 4817 cell (mobile) phone: + 425 314 0199 e-mail address: bade...@gmail.com Web site: www.immunobioscience.com Marketing: phone: + 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: bachelor's degrees
Rene makes an excellent point as to the reason for having a license. I agree that it is a good idea. Unfortunately NYS took the idea and botched it. The NSH tried to push them in the direction of using the HT exam as their qualifying exam, but instead they want to write their own and they are placing unrealistic education requirements. There is only one school in NYS to get an A.S. in histotechnology, and it would require most people in the state to relocate well away from where they have established their careers. I don't think the bureaucrats realize that they are making it difficult for labs to fill positions, and this will only get worse as people retire from the field and there will only be a handful of fresh graduates every year. As it stands now, I will be taking an online program through Indiana University starting in August in order to meet the requirements set for me. Hopefully they will grant me a limited license until I finish the program so that I can continue to work. Nathan Jentsch B.S. Environmental Science Rochester Institute of Technology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
pH meter calibration. Was Re: [Histonet] Alcian Blue
A pH meter must be calibrated hourly or daily, not monthly! In a research environment, with frequently changing chemicals that might damage the glass electrode, you need to calibrate against standard (usually bought) buffers several times within every day. pH meter electrodes are horribly fragile and horribly expensive. Replacing them is a big item in every lab's budget. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Diana McCaig dmcc...@ckha.on.ca Date: Sunday, December 6, 2009 13:49 Subject: [Histonet] Alcian Blue To: histonet@lists.utsouthwestern.edu In the past, when we made up 1% Alcian blue in 3% acetic acid (using bottle distilled water), the pH was always 2.5. We did not have to modify it to get the proper pH. Still using the same powder, we are getting a pH of 2.1. I hate to adjust it because it seems to affect the staining. Any suggestions. The pH meter is calibrated monthly. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paper on osteoclasts
Hi all, I am going crazy - and its not even friday yet! A little while ago (OK about 3 years) i came across a paper where the researcher had shown the transition of macrophages to osteoclasts using immuno staining - i cannot seem to find it on Pubmed, dont know the author date or journal. i hope soemone out there can help best egards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
