Re: [Histonet] paper on osteoclasts
Is this it? http://www.jbmronline.org/doi/full/10.1359/jbmr.2000.15.8.1477?cookieSet=1 I've never read the paper, so I don't know exactly what you're looking for... but I sometimes find Google Scholar to be a better search engine for journal articles (which is what I used to find the above article). Drew On Tue, Dec 8, 2009 at 01:36, louise renton louise.ren...@gmail.com wrote: Hi all, I am going crazy - and its not even friday yet! A little while ago (OK about 3 years) i came across a paper where the researcher had shown the transition of macrophages to osteoclasts using immuno staining - i cannot seem to find it on Pubmed, dont know the author date or journal. i hope soemone out there can help best egards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hi histonetters I was wondering if anyone of you has ever used an antibody against P63 on chicken tissues? I can find a lot of antibodies, but none of these have ever been tested on chicken tissues. If someone has recommendations please feel free to contact me! Thanks a lot. Joost Bruijntjes TNO.NL http://www.tno.nl/ Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijnt...@tno.nl mailto:joost.bruijnt...@tno.nl Disclaimer http://www.tno.nl/tno/email/ This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Working on Ventana IHC Stainers
Hi, I am an histotech working in the US. I am interested in learning the operation of the ventana immunostainers. I will appreciate it, if one of the histology/ihc labs could grant me this great favour. Hoping to hear from you guys asap. Jimmy A ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Advice about human autopsy samples in a purely research core lab
Hi Histoland! I work in a small histology and microscopy core (2 members of staff) in San Francisco at the Gladstone Institutes (affiliated with UCSF). We have recently had an enquiry from a private autopsy service (with LLC in their name) about human autopsy samples for processing, cutting, HE staining and maybe a few special stains here and there. My institutes would like to have a contract in place for this work to cover our bases and I would like some advice on what to include in that contract. I have asked about regulations, and they say that their information is private and not something monitored by outside agencies like CAP, JCAHO, because they are not a diagnostic lab dealing with living patients. They say that JCAHO doesn't apply because they are not delivering health care. They also say that: An autopsy is not a surgical/therapeutic or lab test procedure by any states law except Connecticut. and HIPPA doesn't apply because the patient is deceased. I have worked with human tissue before, I ran a histo lab in London UK so I am well aware of the UK laws, but unfortunately not the US ones. So I ask the histoland: Is this all true? Are there any sticking points we may run into with the regulations? How could we phrase the contract so that there is no chance of come-back? Big questions, I know, but any advice would be gratefully received Yours Caroline Caroline Miller, M.Sc, DIC, AIBMS Manager Histology and Microscopy Core J David Gladstone Institutes 1650 Owens St San Francisco CA 94158 Tel: 415 734 2566 Fax: 415 355 0824 Cell: 415 218 7297 http://www.gladstone.ucsf.edu/gladstone/site/histology/ cmil...@gladstone.ucsf.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TSA
Hi, We are working here with TSA amplification kits, using them in free floating sections or mounted sections. Normally our sections are thick, like 40um. We consistently have a lack of an even distribution of the staining, normally staying in the first couple of microns if the antigen is very abundant (like NeuN) or being more even with very sparse cells. Does anyone know what couls be happening here, how could we solve this? thank you very much. By the way, anyone knows another way to aplify fluorescence signal that does not involve TSA, like ABC kit... Thank you very much ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Stainer
Hi I am looking for a lab that can allow me to spend one or two days to properly see how the ventana autostainer works. I am an histotech working here in the US. I am interested in learning the operation of the ventana immunostainers. I will appreciate it, if one of the histology/ihc labs could grant me this great favour. Hoping to hear from you guys asap. Jimmy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Linear Slide Stainers
Hello... I've been told that the GLX and SLS Linistat linear slide stainers are being discontinued. I was going to order a GLX for our new Mohs lab, and now I'm unsure what to purchase instead. What are others using? Do you need a fume hood? If I go formalin free and xylene free, do I need a hood? I appreciate any suggestions you may have. Thanks much, Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System bauer.ka...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[HISTONET] fixation and storage of tissue for TEM
Dear all, I would like to fix and preserve mouse skin tissue for processing for transmission electron microscopy (TEM) at a later date. I was wondering whether I can store tissue following fixation overnight in 2.5% gluteraldehyde in 0.1M sodium cacodylate buffer and, if so, whether it can be stored indefinitely in this buffer (or whether I need to treat with OsO4 and store at 70% ethanol)? Thanks and best wishes Nick ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
