RE: [Histonet] To my fellow histotechs....
* Cyclin D1 : NeoMarkers, SP4 * CD56 : NeoMarkers, 123C3.D5 * CD138 : Dako, M115 * Kappa : Dako, A0191 (Polyclonal) * Lambda : Dako, A0193 (Polyclonal) * MUM-1 : Dako, MUM1p Willem Hoekert Pathology, OLVG The Netherlands Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Vimentin for Mouse
I believe that Abcam makes a Rabbit anti-Vimentin, we have used in mouse with heat retrieval at ph6 and it worked really well. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: Bell, Pat pat.b...@ucdenver.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 17, 2009 6:00 PM Subject: [Histonet] Vimentin for Mouse Thank you all very much for your help regarding the COX2 for the mouse. Now I would like to ask your help regarding Vimentin for FFPE mouse tissue. I have tried Dako and Sigma with no success. I am also not sure of what clone to use. Thanks again. Pat Pat Bell HT(ASCP) Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edumailto:pat.b...@ucdenver.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH
Is anyone else having trouble with getting the NSH website online? -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NSH webiste problems?
Yes, the website seems to be down. Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson Sent: Friday, December 18, 2009 5:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Is anyone else having trouble with getting the NSH website online? -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Labelers
Dear Histonetters! I was wondering if anyone out there is using a slide labeler. I would appreciate any info on models(serial numbers), and vendors. We have high throughput and need a more automated system with minimal time to input data. Thank you for any suggestions. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide Labelers
Leica IP S Slide Printer - Original Message - From: Igor Deyneko igor.deyn...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Friday, December 18, 2009 10:44 AM Subject: [Histonet] Slide Labelers Dear Histonetters! I was wondering if anyone out there is using a slide labeler. I would appreciate any info on models(serial numbers), and vendors. We have high throughput and need a more automated system with minimal time to input data. Thank you for any suggestions. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP Schedules
Under separate cover I am sending the information you need (schedules and xylene substitute). rené J. --- On Fri, 12/18/09, Sheila Haas micropathl...@yahoo.com wrote: From: Sheila Haas micropathl...@yahoo.com Subject: [Histonet] VIP Schedules To: histonet@lists.utsouthwestern.edu Date: Friday, December 18, 2009, 12:03 PM Would anyone be willing to share a shortened processing schedule to use with the VIP? I have one but would like to compare with what others are doing. I'm also looking for xylene free alternatives for the VIP and schedules for that type of processing. Any assistance or reference would be appreciated. Thanks in advance! Sheila Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ASCP website
Has anyone had problems on ASCP's website in the last couple of days? I have been trying to enter stuff in on re.member and it has been timing out or not allowing logging in at all...havent' had any problems on NSH's website TGIF! Laurie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IGF1R beta recommendations anyone?
Happy Holidays everyone, I am looking for an IGF1R beta antibody that works well in human liver and pancreatic tissue. I have one here that I have tried and it worked well on the pancreas and nothing for the liver. Suggestions? Happy Friday! Laurie Laurie Popp, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Silver Nitrate instead of inking??
I've worked in about 60 pathology operations since 1964. I have NEVER seen silver nitrate used for tissue marking, though I've heard of it. I think the point about the silver fouling processors is well taken. In addition, silver has the nasty habit of forming explosive complexes - I don't want to think about it reacting with Bouin's fixative to form silver picrate. There are basically three options. India ink - $4 a bottle from your local crafts store. Special marking inks, of which the Davidson marking inks are perhaps the most widely used, and in my opinion the best (I have no commercial connection with Mrs. Stewart, much less with Martha Stewart). The third option is tattoo inks - cheap, available in an unlimited variety of colors, and in my limited experience with them they get the job done. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Effects of Inking on Special stains or IHC
Hello All, One of my pathologists asked the following question. Does inking tissue effect special staining or IHC results? I replied that I had never heard of this and not to my knowledge. I thought that if the chromagen was the same color of the dye then there could be some difficulty in deciphering between the two. Therefore, I am wondering if anyone has experienced a problem. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Effects of Inking on Special stains or IHC
We use six different colors of marking inks (black, blue, purple, yellow, green, orange)and have not noticed any interference on IHC. We did decide not to use red ink since we were concerned it might make the Vulcan fast red chromogen used in our PIN4 cocktail hard to interpret. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matt Brooks Sent: Friday, December 18, 2009 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Effects of Inking on Special stains or IHC Hello All, One of my pathologists asked the following question. Does inking tissue effect special staining or IHC results? I replied that I had never heard of this and not to my knowledge. I thought that if the chromagen was the same color of the dye then there could be some difficulty in deciphering between the two. Therefore, I am wondering if anyone has experienced a problem. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ Information from ESET NOD32 Antivirus, version of virus signature database 4700 (20091218) __ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __ Information from ESET NOD32 Antivirus, version of virus signature database 4700 (20091218) __ The message was checked by ESET NOD32 Antivirus. http://www.eset.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Silver Nitrate instead of inking??
We in our lab use regular household white vinegar to help the ink stay on the tissue during processing, cheap and safe. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond [rsrichm...@gmail.com] Sent: Friday, December 18, 2009 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Silver Nitrate instead of inking?? I've worked in about 60 pathology operations since 1964. I have NEVER seen silver nitrate used for tissue marking, though I've heard of it. I think the point about the silver fouling processors is well taken. In addition, silver has the nasty habit of forming explosive complexes - I don't want to think about it reacting with Bouin's fixative to form silver picrate. There are basically three options. India ink - $4 a bottle from your local crafts store. Special marking inks, of which the Davidson marking inks are perhaps the most widely used, and in my opinion the best (I have no commercial connection with Mrs. Stewart, much less with Martha Stewart). The third option is tattoo inks - cheap, available in an unlimited variety of colors, and in my limited experience with them they get the job done. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Effects of Inking on Special stains or IHC
Your answer was the correct answer. René J. --- On Fri, 12/18/09, Matt Brooks mbro...@incytepathology.com wrote: From: Matt Brooks mbro...@incytepathology.com Subject: [Histonet] Effects of Inking on Special stains or IHC To: histonet@lists.utsouthwestern.edu Date: Friday, December 18, 2009, 3:19 PM Hello All, One of my pathologists asked the following question. Does inking tissue effect special staining or IHC results? I replied that I had never heard of this and not to my knowledge. I thought that if the chromagen was the same color of the dye then there could be some difficulty in deciphering between the two. Therefore, I am wondering if anyone has experienced a problem. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Silver Nitrate instead of inking??
I did work in a lab that used silver nitrate to mark cervical biopsies. Most of the pathologists used a 1% solution, often dipping the whole cervix into it and causing the whole lot to precipitate from the formalin (grrr). One used four or five silver nitrate sticks by dipping in water and just rubbing over the specimen. It does work, a black porecipitate coating the edge. I have never heard of any safety concerns with it as the specimen was placed back into formalin immediately after the coating for processing and reduction seems to be total. What I did note was that the silver nitrate sometimes penetrated the tissue and you could see s rim of fine granules about a cell deep into the surface which could be distracting. I then got them to switch to India ink, but I found that messy, so I switched again to Blueing, which was relatively clean and convenient. I also obtained some tattoo inks so different surfaces could be marked if necessary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Silver Nitrate instead of inking??
Loralee McMahon, HTL (ASCP), Immunohistochemistry Supervisor, Department of Surgical Pathology, Strong Memorial Hospital, Rochester NY notes: We in our lab use regular household white vinegar to help the ink stay on the tissue during processing, cheap and safe. I forgot to mention this, because I personally don't use it - I find that if you take the time to blot an unfixed or fixed specimen thoroughly dry before inking, the ink stays on quite satisfactorily. But if the pathologist does want to use something, then 3% acetic acid (which can be ordinary household white vinegar diluted 1 to 1 with water) should be offered. Don't use Bouin's fixative (we all know the varied problems presented by picric acid), nor acetone (flammable). Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Histonetters, Does anyone out there in histoland have a job description in their lab for staff that are like aides or assistants? They would be entering specimens, distributing slides, logging in placentas, tossing specimens, and just basically assisting the technical staff in any way possible. It would not require a college degree. I was wondering what you might call that job description? And would it be possible for you to share your explanation and requirements for a job similar to the one I described? Thank you ahead of time for any examples you have. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknut...@primecare.org mailto:dknut...@primecare.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laura Miller is Out of the Office.
I will be out of the office starting 12/18/2009 and will not return until 12/29/2009. I will repsond to your message when I return on December 29th! HAPPY HOLIDAYS! __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[histonet] Muscle
Dear All, I was wondering whether anyone had a good recommendation for a textbook on the physiology and biochemistry of muscle? Also I was wondering whether anyone could recommend a good antibody for myosin heavy chain, fast type (type I)? Best wishes Nick ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide labeler
Igor - We have a Leica slide labeler. It is easy to use, but we have found that you should use slides with 90 degree corners and a rough surface. Slides with clipped corners tend to slide through the chute and get caught inside the machine. It's easy to extract them but it's a hassle. Also the ink and light bulb are expensive, but we wouldn't give ours up for anything. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide labeler
And if you have their coverslipper, it doesn't like the rounded edges either!! Colleen Forster. Cheryl Crowder wrote: Igor - We have a Leica slide labeler. It is easy to use, but we have found that you should use slides with 90 degree corners and a rough surface. Slides with clipped corners tend to slide through the chute and get caught inside the machine. It's easy to extract them but it's a hassle. Also the ink and light bulb are expensive, but we wouldn't give ours up for anything. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
