Re: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome
i spent many many years handling pig, mouse and human brain, cutting sections on 2x3 slides. the trick is to use the tap water float out before the warm water, also to cut sections thicker than normal - cut at 10-15 and maybe even 20 microns - trouble is at that thickness AND it being animal tissue, the sections wash off in a flash - unless you use adhesive and cook the sections on to the slides gently at 32C for at least overnight i have some 20micron silver stained cerebellum on 2x3 slides here with me right now, 20 years later they are still good as new!! good luck Annie 2009/12/30 Gudrun Lang gu.l...@gmx.at Have you tried to float the section first in a roomtemp. waterbath (bowl with tapwater)? There you can flatten the wrinkles with a brush, then mount it on the glass slide and bring it into the warm water. While gliding from the glass you can also use the brush to pull softly on the section to stretch it. If the infiltrated tissue is still too soft, I suggest longer infiltration time in xylen and paraffin to get rid of the fatty part of brain tissue. Sometimes even cutting on next day of embedding (one day waiting) helps. Do you cool the blocks before cutting? Long enough? For big blocks we usually use one blade for trimming and a new one for getting a thin section. Hope this helps Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von R C Gesendet: Dienstag, 29. Dezember 2009 23:16 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38). In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Probe for mouse Y chromosome for FISH
Hi Mira, Doubtless quite a few companies out there. Try Cambio: http://www.cambio.co.uk/catalogue-Star_FISH_copy_Mouse_Chromosome_Specific_Probes_Biotin_Labelled_br_Concentrated_Format-1-426 Conc Mouse WCP Biotin Chromosome Y Catalogue number 1187-YMB-02 Have a great 2010 Peter Peter Tree Account Manager Custom Antibody Generation AbD Serotec - Your first choice for antibodies! (A Division of MorphoSys) Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE. Tel: +44 (0)1865 852700 Fax: +44 (0)1865 373899 Direct: +44 (0)1865 852724 pt...@ab-direct.com www.ab-direct.com Before you PRINT this e-mail, think about the ENVIRONMENT. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of birnba...@asaf.health.gov.il Sent: Wednesday, December 30, 2009 10:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Probe for mouse Y chromosome for FISH Dear all We look for Fluorescence probe of mouse Y chromosome. Which company does sale it? Mira Birnbaum Pathology Asaf Hrofeh Medical Center Israel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet MorphoSys UK Ltd., Registered in England No: 1604642 Registered office: Greyfriars Court, Paradise Square, Oxford OX1 1BB, UK This message may contain confidential and/or privileged information. If you are not the addressee or authorized to receive this for the addressee, you must not use, copy, disclose or take any action based on this message or any information herein. If you have received this message in error, please advise the sender immediately by reply e-mail and delete this message. Thank you for your cooperation. Diese E-Mail kann vertrauliche und/oder rechtlich geschuetzte Informationen enthalten. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail irrtuemlich erhalten haben, informieren Sie bitte sofort den Absender und vernichten Sie diese Mail. Das unerlaubte Kopieren sowie die unbefugte Weitergabe dieser Mail ist nicht gestattet. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin pigment?
First you should acquire a field micrometer. One of those that have all the field of view covered by s lattice of crossed lines forming squares. Using a low magnification objective (10:1) count in how many squares you see the pigment and calculate in what % of the total number of squares they are. Repeat in at least 10 areas of the section selected at random, and you will have a percentage idea of how much pigment is in your section for the time the specimen was kept in formalin at any given concentraiton. René J. --- On Wed, 12/30/09, Adeluwoye Oluwatosin princekun...@gmail.com wrote: From: Adeluwoye Oluwatosin princekun...@gmail.com Subject: [Histonet] Formalin pigment? To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 30, 2009, 7:56 PM Hello, please i am working effects of various concentration of formaldehyde on liver tissue. The sections shows some degree of formalin pigmentation form 20 to 35% and concentrated formaldehyde. Can anyone ofer advise as to how to quantittate o judge the varying degree of pigmentation? Tosin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FNA code
There is no Technical component to 88173, so how do you charge for the technical preparation of the ThinPrep? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmc...@lakelandregional.org dkb...@chs.net 12/30/2009 9:42 AM 88173 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Demarinis, Carolyn cdemari...@saratogacare.org Sent by: histonet-boun...@lists.utsouthwestern.edu 12/30/2009 08:18 AM To histo...@pathology.swmed.edu cc Subject [Histonet] FNA code Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at priv...@saratogacare.org and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:mmc...@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK;PREF:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:mmc...@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Nail softner
Hi Everyone, I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm hoping there are other alternatives you all might be using that you can share with me. What we had has lasted us my whole time here and then some so I havent had to order it before. Any suggestions would be so greatly appreciated. Thank you in advance. You all have a Happy New Year Delia Lead Histologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat
We are a Derm Lab and we use ours quite a bit. We do a manual defrost monthly. We clean it daily. It is also set to auto defrost nightly. Delia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: Nail softner
Under separate cover, the best procedure ever! René J. --- On Thu, 12/31/09, DELIA GARCIA deliad...@yahoo.com wrote: From: DELIA GARCIA deliad...@yahoo.com Subject: [Histonet] Nail softner To: histonet@lists.utsouthwestern.edu Date: Thursday, December 31, 2009, 2:26 PM Hi Everyone, I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm hoping there are other alternatives you all might be using that you can share with me. What we had has lasted us my whole time here and then some so I havent had to order it before. Any suggestions would be so greatly appreciated. Thank you in advance. You all have a Happy New Year Delia Lead Histologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FNA code
Mary, I'd have to disagree. 88173 has both a technical and professional component. This is well documented in a resource your dept might find very beneficial entitled Pathology Service Coding Handbook which is authored by Dennis Padgett, a noted coding authority. It is customary to have a cytotechnologist screen FNA smears or Thin Prep slides prior to the slides going to sign out with the pathologist. And of course the hospital incurs supply expenses as well as labor expense associated with the preparation of the specimen(s) that will be interpreted The rule of thumb is as follows: 88173 should be dropped for every Fine Needle Aspirate. This represents all of the work in the laboratory (including prep and staining) that leads to the interpretation both technical and professional 88172 is dropped only if the procedure is attended by a pathologist or cytotechnologist to render an adequacy during the procedure. This presumes that the aspirate is not being performed by the pathologist but another clinician, radiologist, etc. It is appropriate to drop additional codes to 88173, such as codes for cell block (88305), special stains (88312;88313), IHC (88342) or thin layer preparation of the aspirate (88112 ) however it may interest you to know that, according to Padgett, Medicare will never pay 88112 when it is added onto 88173. so for example, if you do direct smears and thin prep on an FNA specimen, Medicare will still only pay for 88173. Other payors may vary and for simplicity it may be simpler for labs to drop 88112 whenever thin prep is done on a FNA specimen without regard to payor however you ought to consult with your institution's legal or compliance officer. I highly recommend the reference I mentioned above. You wouldn't believe the complexity of the interpretations for these codes and Padgett points out instances where CAP's interpretation disagrees with AMA's. Padgett devotes 21 pages of discussion on FNA coding Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary McCoy Sent: Thursday, December 31, 2009 10:23 AM To: dkb...@chs.net; histonet-boun...@lists.utsouthwestern.edu; Carolyn Demarinis Cc: histo...@pathology.swmed.edu Subject: Re: [Histonet] FNA code There is no Technical component to 88173, so how do you charge for the technical preparation of the ThinPrep? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmc...@lakelandregional.org dkb...@chs.net 12/30/2009 9:42 AM 88173 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Demarinis, Carolyn cdemari...@saratogacare.org Sent by: histonet-boun...@lists.utsouthwestern.edu 12/30/2009 08:18 AM To histo...@pathology.swmed.edu cc Subject [Histonet] FNA code Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at priv...@saratogacare.org and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
Re: [Histonet] Nail softner
20% sodium hydroxide works great. Hey, they don't call me Joe the Toe for nothing. Happy New Year JTT - Original Message - From: DELIA GARCIA deliad...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 31, 2009 1:26 PM Subject: [Histonet] Nail softner Hi Everyone, I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm hoping there are other alternatives you all might be using that you can share with me. What we had has lasted us my whole time here and then some so I havent had to order it before. Any suggestions would be so greatly appreciated. Thank you in advance. You all have a Happy New Year Delia Lead Histologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Nail softner
We used to put our nails in Nail Hair remover for over night. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DELIA GARCIA [deliad...@yahoo.com] Sent: Thursday, December 31, 2009 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail softner Hi Everyone, I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm hoping there are other alternatives you all might be using that you can share with me. What we had has lasted us my whole time here and then some so I havent had to order it before. Any suggestions would be so greatly appreciated. Thank you in advance. You all have a Happy New Year Delia Lead Histologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
