Re: [Histonet] Gill sectioning tips?
Are your specimens from cartilaginous or bony fishes? Ages ago I worked with head and neck specimens of quite big (10 cm) goldfishes (bony) in a study of optic nerve regeneration. Decalcification after adequate formaldehyde fixation permitted easy cutting of near-serial paraffin sections containing bone, brain and incidentally gills, skin (which has bony scales) and other entertaining things not seen in sections of small people or other little mammals. I don't know how to soften really tough cartilage. Does anyone? John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Cammi Thornton cehic...@olemiss.edu Date: Tuesday, February 9, 2010 16:05 Subject: [Histonet] Gill sectioning tips? To: histonet@lists.utsouthwestern.edu Hi everyone, We have been trying to section gills lately and have come across quite a few problems. Does anybody have any helpful hints for fixing/sectioning gills? We are going to try to separate each individual arch but the fish we use are very small so we don't know how that will go. Thanks, Cammi Cammi Hickman Thornton Research Development Chemist University of Mississippi School of Pharmacy Department of Pharmacology 200 Old Power Plant University, Mississippi 38677 Phone - 662-915-7612, Fax - 662-915-5148 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peloris Processor Users
Could you please share your experiences with me? We have had this processor in our facility since 2007, but I am curious about other facilities experiences. I thank you in advance, Sheila Tapper HT(ASCP) Anatomic Pathology Supervisor SMDC Clinical Laboratory 407 East First Street Duluth, MN 55804 This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene Recycler
Anyone using the new CBG instrument? Am having some issues with clogged lines - which we never had with the older model. Really don't want to change processes - which is what they are asking us to do. Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peloris processor
We are also interested in other's experiences with the Peloris processor. We are considering a rapid tissue processor and would like to hear from others who have rapid tissue processors. What type do you have? And please share your pros and cons. Thank you very much for your help. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknut...@primecare.org mailto:dknut...@primecare.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] problem with subbed slides
dear histonetters following gelatin subbing of big glass slides we observed numerous white patches in almost the entire batch of slides subbed. what could be the remedy to remove these white patches. waiting for a practical solution. with regards hisham mohammed senior research centre national brain research centre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Liver Tissue
Content-Type: text/plain; CHARSET=US-ASCII Are your specimens from cartilaginous or bony fishes? Ages ago I worked with head and neck specimens of quite big (10 cm) goldfishes (bony) in a study of optic nerve regeneration. Decalcification after adequate formaldehyde fixation permitted easy cutting of near-serial paraffin sections containing bone, brain and incidentally gills, skin (which has bony scales) and other entertaining things not seen in sections of small people or other little mammals. I don't know how to soften really tough cartilage. Does anyone? John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Cammi Thornton cehic...@olemiss.edu Date: Tuesday, February 9, 2010 16:05 Subject: [Histonet] Gill sectioning tips? To: histonet@lists.utsouthwestern.edu Hi everyone, We have been trying to section gills lately and have come across quite a few problems. Does anybody have any helpful hints for fixing/sectioning gills? We are going to try to separate each individual arch but the fish we use are very small so we don't know how that will go. Thanks, Cammi Cammi Hickman Thornton Research Development Chemist University of Mississippi School of Pharmacy Department of Pharmacology 200 Old Power Plant University, Mississippi 38677 Phone - 662-915-7612, Fax - 662-915-5148 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 20 Date: Wed, 10 Feb 2010 10:54:04 -0600 From: Tapper, Sheila J. stap...@smdc.org Subject: [Histonet] Peloris Processor Users To: histonet@lists.utsouthwestern.edu Message-ID: a72cc3753325e4448ae0a7a3c46ad6db056e0...@boreal.ntcampus.smdc.org Content-Type: text/plain; charset=us-ascii Could you please share your experiences with me? We have had this processor in our facility since 2007, but I am curious about other facilities experiences. I thank you in advance, Sheila Tapper HT(ASCP) Anatomic Pathology Supervisor SMDC Clinical Laboratory 407 East First Street Duluth, MN 55804 This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. -- Message: 21 Date: Wed, 10 Feb 2010 11:58:21 -0600 From: Nita Searcy nsea...@swmail.sw.org Subject: [Histonet] Xylene Recycler To: histonet@lists.utsouthwestern.edu Cc: Patricia Webster pwebs...@swmail.sw.org Message-ID: 4b729f5c.5d38.00e...@swmail.sw.org Content-Type: text/plain; charset=us-ascii Anyone using the new CBG instrument? Am having some issues with clogged lines - which we never had with the older model. Really don't want to change processes - which is what they are asking us to do. Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 -- next part -- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 75, Issue 14 __ Information from ESET NOD32 Antivirus, version of virus signature database 4854 (20100210) __ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __ Information from ESET NOD32 Antivirus, version of virus signature database 4855 (20100210) __ The message was checked by ESET NOD32 Antivirus. http://www.eset.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Fish gills
We had superb sections of fish gills after GMA embedding - 2 to 3 um thick sections cut with glass knives, and an HE stain. The gills were separated but if your samples are very small, no more than 1 mm or so thick, GMA should work. Our departmental photographer won a major scientific photographic contest with her photomicrographs of these gills showing parasites attached to the gills. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fish Gills
Cammi - Our lab processes thousands of fish specimens yearly. Fish of all ages. If the fish is initially fixed in Davidson's there should be no need for decalcification. Older, larger fish, just like mammals, may take further softening. If you have specimens already processed that don't cut well, after facing off soak them in Downy for an hour or so. The first sections should be really good. If you have any questions, contact me directly. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Crystal identification
Are any of you out there in histoland doing smears for crystal identification (Gout). If so how long are you retaining the slides and how do you preserve the slides? Thanks raj ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LAMBDA AND KAPPA STAINING PROBLEMS
Hi, We are having overstaining problems with both our Kappa and Lambda stains using our recently purchased Benchmark XT. Pls, I will appreciate it, if you guys at histoland could offer some suggestions as to taking care of this problem. Thanking you all for your usual cooperation. Adesuyi A, BS,HT(ASCP), HTL(ASCP) _ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469227/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem with subbed slides
Just for interest's sake could this be some fungal growth? Try staining a slide with H/E and look under the microscope. how to fix? perhaps acid digestion and resubbing? On Wed, Feb 10, 2010 at 8:15 PM, Hisham Mohammed hisham@gmail.comwrote: dear histonetters following gelatin subbing of big glass slides we observed numerous white patches in almost the entire batch of slides subbed. what could be the remedy to remove these white patches. waiting for a practical solution. with regards hisham mohammed senior research centre national brain research centre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
