[Histonet] FW: mouse prions
Anybody out there handling/processing/sectioning mouse tissue containing mouse prions, if so, what safety precautions do you take?. Thanks Richard Edwards ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New cryostat decontamination CAP statndard
Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP.12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. - The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Work Load Units
What do you exactly mean by Work Load Units? You should know how many cases and blocks you process by day, week or year, and you also know how many hours your staff uses to complete the tasks derived from those cases and blocks. The total work completed between the staff members completing them during a given amount of time will allow you to calculate productivity. If you are referring to which to select, the case is relevant for the pathologists, the slide for the cytotech and the block for the histotech because they refer to what the usually handle. René J. --- On Mon, 2/22/10, Fierke, Vaughn vaughn.fie...@va.gov wrote: From: Fierke, Vaughn vaughn.fie...@va.gov Subject: [Histonet] Work Load Units To: histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 6:01 PM Looking for a good system that works in recording Work Load Units. I've inquired to CAP; they do not have any material available at this time nor recommendations. I've looked at CPT codes but they only reflect billable services in pathology; descriptions are fairly general and cannot be broken down into tasks of the tech. Looked in the Histonet archives; found information not too current. Thanks for any information. Vaughn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PPE's embedding and cutting
No! Neither to wear gloves when sectioning. Both are unwarranted requirements that interfere with the histotech's productivity. René J. --- On Mon, 2/22/10, rick.garnh...@memorialhealthsystem.com rick.garnh...@memorialhealthsystem.com wrote: From: rick.garnh...@memorialhealthsystem.com rick.garnh...@memorialhealthsystem.com Subject: [Histonet] PPE's embedding and cutting To: histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 6:12 PM Anyone in histology land required to wear all PPE's to embed and cut? Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] New cryostat decontamination CAP statndard
Thank you Jeffery. I am not sure how we get to CAP on this however; it makes no sense for a really busy lab unless they want to give us enough cryostats to make up for the problem this will cause. Is this something NSH might decide to help with? It may be time to bombard them (CAP) with questions and report the issues it will cause with patient care. None of us want to use a contaminated cryostat but we also know how to decontaminate without being in danger and stopping surgery for frozens . Pam Marcum UAMS - Anatomic Pathology - Original Message - From: Jeffrey Silverman JSilverman @ NSHS . edu To: histonet @lists. utsouthwestern . edu Cc: Jeffrey Silverman JSilverman @ NSHS . edu Sent: Tuesday, February 23, 2010 8:23:39 AM GMT -06:00 US/Canada Central Subject: [ Histonet ] New cryostat decontamination CAP statndard Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP .12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. - The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. ___ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New cryostat decontamination CAP statndard
The way I read this is if I don't use my cryostat 7 days a week, 365 days a year, then I don't use it daily and therefore I will set my own routine decontamination schedule. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Tuesday, February 23, 2010 9:06 AM To: Jeffrey Silverman Cc: histonet@lists.utsouthwestern.edu; Jeffrey Silverman Subject: Re: [Histonet] New cryostat decontamination CAP statndard Thank you Jeffery. I am not sure how we get to CAP on this however; it makes no sense for a really busy lab unless they want to give us enough cryostats to make up for the problem this will cause. Is this something NSH might decide to help with? It may be time to bombard them (CAP) with questions and report the issues it will cause with patient care. None of us want to use a contaminated cryostat but we also know how to decontaminate without being in danger and stopping surgery for frozens . Pam Marcum UAMS - Anatomic Pathology - Original Message - From: Jeffrey Silverman JSilverman @ NSHS . edu To: histonet @lists. utsouthwestern . edu Cc: Jeffrey Silverman JSilverman @ NSHS . edu Sent: Tuesday, February 23, 2010 8:23:39 AM GMT -06:00 US/Canada Central Subject: [ Histonet ] New cryostat decontamination CAP statndard Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP .12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. - The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. ___ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] New cryostat decontamination CAP statndard
Please remember that from time to time CAP inspectors sit to ponder what something new can we add to our list so it appears that we are not using always the same old one?. And from time to time the come up with requirements like this. You will never change them and the requirements will keep piling up one on top of the other. Caveat: sometimes the changes are warranted (only some). René J. --- On Tue, 2/23/10, Pamela Marcum mucra...@comcast.net wrote: From: Pamela Marcum mucra...@comcast.net Subject: Re: [Histonet] New cryostat decontamination CAP statndard To: Jeffrey Silverman jsilver...@nshs.edu Cc: histonet@lists.utsouthwestern.edu, Jeffrey Silverman jsilver...@nshs.edu Date: Tuesday, February 23, 2010, 10:05 AM Thank you Jeffery. I am not sure how we get to CAP on this however; it makes no sense for a really busy lab unless they want to give us enough cryostats to make up for the problem this will cause. Is this something NSH might decide to help with? It may be time to bombard them (CAP) with questions and report the issues it will cause with patient care. None of us want to use a contaminated cryostat but we also know how to decontaminate without being in danger and stopping surgery for frozens . Pam Marcum UAMS - Anatomic Pathology - Original Message - From: Jeffrey Silverman JSilverman @ NSHS . edu To: histonet @lists. utsouthwestern . edu Cc: Jeffrey Silverman JSilverman @ NSHS . edu Sent: Tuesday, February 23, 2010 8:23:39 AM GMT -06:00 US/Canada Central Subject: [ Histonet ] New cryostat decontamination CAP statndard Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP .12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. - The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. ___ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Work Load Units
Unfortunately, many facilities are only counting billable tests and all the recuts, sendouts, and work done pulling cases for conferences cannot be counted. It is a nightmare that needs to be addressed!! My 2 cents...j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 23, 2010 09:47 To: histonet@lists.utsouthwestern.edu; VaughnFierke Subject: Re: [Histonet] Work Load Units What do you exactly mean by Work Load Units? You should know how many cases and blocks you process by day, week or year, and you also know how many hours your staff uses to complete the tasks derived from those cases and blocks. The total work completed between the staff members completing them during a given amount of time will allow you to calculate productivity. If you are referring to which to select, the case is relevant for the pathologists, the slide for the cytotech and the block for the histotech because they refer to what the usually handle. René J. --- On Mon, 2/22/10, Fierke, Vaughn vaughn.fie...@va.gov wrote: From: Fierke, Vaughn vaughn.fie...@va.gov Subject: [Histonet] Work Load Units To: histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 6:01 PM Looking for a good system that works in recording Work Load Units. I've inquired to CAP; they do not have any material available at this time nor recommendations. I've looked at CPT codes but they only reflect billable services in pathology; descriptions are fairly general and cannot be broken down into tasks of the tech. Looked in the Histonet archives; found information not too current. Thanks for any information. Vaughn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
madelin...@yahoo.com Madeline Rotger Milanese H.T. 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New cryostat decontamination CAP statndard
To me the key word in here is interval. If a regularly scheduled interval is set, this could be daily , weekly, monthly or quarterly. Obviously there are going to be the times when a contaminant is there and the machine has to be brought down, but also I do not see the mention of the UV light. We have a Leica that has an internal UV button. This is pressed everyday as well as normal daily maintenance. Once a month our cryostat is brought down for decontamination. This is part of the normal maintenance. I agree that is the CAP wants this to happen, it needs to be clearly defined within the question and not up to interpretation. Jesus Ellin HT/PA ASCP -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, February 23, 2010 8:18 AM To: Pamela Marcum; Jeffrey Silverman Cc: histonet@lists.utsouthwestern.edu; Jeffrey Silverman Subject: RE: [Histonet] New cryostat decontamination CAP statndard The way I read this is if I don't use my cryostat 7 days a week, 365 days a year, then I don't use it daily and therefore I will set my own routine decontamination schedule. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Tuesday, February 23, 2010 9:06 AM To: Jeffrey Silverman Cc: histonet@lists.utsouthwestern.edu; Jeffrey Silverman Subject: Re: [Histonet] New cryostat decontamination CAP statndard Thank you Jeffery. I am not sure how we get to CAP on this however; it makes no sense for a really busy lab unless they want to give us enough cryostats to make up for the problem this will cause. Is this something NSH might decide to help with? It may be time to bombard them (CAP) with questions and report the issues it will cause with patient care. None of us want to use a contaminated cryostat but we also know how to decontaminate without being in danger and stopping surgery for frozens . Pam Marcum UAMS - Anatomic Pathology - Original Message - From: Jeffrey Silverman JSilverman @ NSHS . edu To: histonet @lists. utsouthwestern . edu Cc: Jeffrey Silverman JSilverman @ NSHS . edu Sent: Tuesday, February 23, 2010 8:23:39 AM GMT -06:00 US/Canada Central Subject: [ Histonet ] New cryostat decontamination CAP statndard Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP .12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. - The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. ___ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] seeking histology position - Ron Martin
Go to https://jobs.ufl.edu/applicants/jsp/shared/frameset/Frameset.jsp?time=1266864723194 You'll have to create a user ID and login. UF will have a posting in the next week or so. ASCP license desired, FL DOH license is a plus. Also the Gainesville Veterans hospital has a position available - you can apply in person but I am not sure where to apply online. I hope you are successful in your job search! Kelley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New cryostat decontamination CAP statndard
Folks: Please read the checklist notes carefully- I don't think for most of us this is open to interpretation. The standard note says: MUST be defrosted and decontaminated with a tuberculocidal WEEKLY for machines used daily. It's not open to interpretation if you do frozens daily. Tiny institutions that do one or two frozens a week may have some leeway, but I suspect most of us need to do it weekly to comply with the standard. And the UV lights are not tuberculocidal and do not reach all surfaces. See this link from the Leica instruction manual: http://www.well.ox.ac.uk/_asset/file/leica-disinifection-2.pdf. A partial quote: Please note that ALL visible contamination must be removed from the chamber before using the UV C light and that the germicidal effect of the radiation is restricted to directly illuminated areas. Exposure to UV irradiation cannot replace chemical disinfection. 5. For more extensive disinfection, bring the cryostat to room temperature and wipe the exposed surfaces with an EPA approved tuberculocidal disinfectant. The EPA publishes a list of approved disinfectants. Follow the directions written on the containers. Another reference re: UV decon: from -- http://www.unmc.edu/media/ibc/manual/biosafety_manual_march2008_final_compressed.doc#engineering Radiation Gamma and X-ray are two principal types of ionizing radiation used in sterilization. Their application is mainly centered on the sterilization of prepackaged medical devices. Ultraviolet (UV) radiation is a practical method for inactivating viruses, Mycoplasma, bacteria and fungi. UV radiation is successfully used in the destruction of airborne microorganisms; UV light sterilizing capabilities are limited on surfaces because of its lack of penetrating power. Finally, here's an article on biosafety in pathology labs from CAP Today where they reiterate the weekly requirement for cryostat decontamination at room temperature. http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=cap_today%2F0909%2F0909b_taking_stock.html_state=maximized_pageLabel=cntvwr I can just hear rhe sounds of numerous CAP dings being registered. Oh, the humanity. :-) Jeff Silverman -Original Message- From: Behnaz Sohrab [mailto:sohra...@ah.org] Sent: Tuesday, February 23, 2010 11:01 AM To: Silverman, Jeffrey Subject: Re: [Histonet] New cryostat decontamination CAP statndard I think we can establish our own QC definition for ROUTINE as they are requesting, it could be once a month or any other routine that works for your lab usage and size? Behnaz Silverman, Jeffrey jsilver...@nshs.edu 2/23/2010 6:23 AM Here is the new standard for cryostat decontamination. No references are given documenting or supporting the need for this onerous and cumbersome weekly requirement. I suggest we lobby the CAP and demand both documentation supporting the need for weekly defrosting or, better, a more user friendly and sensible standard. Jeff Silverman **REVISED** 06/15/2009 ANP.12087 Phase II N/A YES NO Is there a documented procedure for the routine decontamination of the cryostat at defined intervals, and are decontamination records evident? NOTE: The cryostat must be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. - The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fwd: Re: [Histonet] Bone tissue
Just for the record, Mollifex isn't alkaline. It is probably either Baker's 1941 mixture (see below) or the following (parts by volume): 95% ethanol 952 Glycerol 32 Acetone 80 Liquid phenol 8 - - - Liquid phenol is probably liquefied phenol, USP, which contains about 10% water and at least 89% phenol (w/w), or something similar. For more information and discussion, see Maria Wynnchuk (1992) Minimizing artifacts in tissue processing: Part 1. Importance of softening agents. J. Histotechnol. 15(4): 321-323. This paper also discusses effects of softening faced blocks on HE staining. John R Baker (1941) used 9 parts 60% ethanol and 1 part glycerol (J. Roy. Microsc. Soc. 61: 75), whereas R. C. Pearlman and Buell C. Cole (1951) sang the praises of 1% dishwashing detergent and similar solutions (Stain Technol. 26(2): 115-118). Manfred Gabe (Histological Techniques, Paris, 1976, a great book) favoured soaking faced paraffin blocks in cold water. For what it's worth, I have found water helpful when sectioning decalcified rats' heads. With all these well documented and inexpensive softening liquids it should never be necessary to resort to a proprietary product whose composition is kept secret from the buyer and user. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Rene J Buesa rjbu...@yahoo.com Date: Monday, February 22, 2010 16:43 Subject: Re: [Histonet] Bone tissue To: histonet@lists.utsouthwestern.edu, Reuel Cornelia reuel.corne...@tsrh.org First of all, Mollifex or any other alkaline substance will do nothing useful to the bone. I tend to think that you processed the bone before it was completely decalcified and that is the cause for an incomplete infiltration and a subsequent difficult sectioning. René J. --- On Mon, 2/22/10, Reuel Cornelia reuel.corne...@tsrh.org wrote: From: Reuel Cornelia reuel.corne...@tsrh.org Subject: [Histonet] Bone tissue To: histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 4:36 PM We have a difficulty cutting metatarsal bone . It seems that our sections are so dried up. I was thinking that our dehydration have something to do with this which we have placed it in a wrong processing procedure for our large bone. The tissue is 4 mm thick and 1-2 cm in length and width and was dehydrated in 70% - 4 hrs, 80%-4 hrs,95% -4 hrs and 2 changes of 100% 3 hrs each, paraffin is 4 hrs each 2 changes. The tissue was decalcified in 14% EDTA. When we start cutting them it is so brittle and we could not even create a section. I have surfaced decal it and also place in a softener Mollifex some of it work but some does not work. Please help us remedy this tissue. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] DRGs histogel
I think dehydration during processing can effect the outcome with histogel. I use it frequently to help orient small samples with very good results almost always. One time, I was trying to arrange several samples in the same block and the histogel got too cold and set up when I was partly finished. I put it all in our oven hoping to remelt it. When I took it out, it had become a hard thin sheet, sort of like dried paint or glue. The only thing I could think of that had happened, was that since it was in a shape with a lot of surface area, it had dried out instead of melting. I wanted to save the sample that was in it, so I added some water. Thankfully, the histogel became gel like again and I was able to find the sample which I transfered to fresh histogel. The reconstituted histogel seemed to be the same as regular histogel, excep it lost its pink color. The samples also were ok. I've never had any real problems cutting histogel (and hope I don't), but if I get one that is a little crumbly, I soak it extra long and that helps. I think that maybe when there are inconsistent results with histogel, maybe something happens during processing so it becomes dried out or overly dehydrated. I have used it both out of formalin and out of 70%. I've also embedded it both wet and dry without problems. Sometimes it is whiter than others. Karen Doty Date: Mon, 22 Feb 2010 09:35:10 -0800 From: dunat...@sbcglobal.net To: port...@msu.edu; algra...@email.arizona.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] DRGs CC: I also think that it is strange of the way Histogel processes. I have posted on the Histonet previously about this exact problem. I worked with Jennifer Hofecker when she was at Vanderbilt U.(sent her my Histogel and she sent me hers) and ended up with perfectly processed Histogel blocks at our facility and hers. I processed a couple of blocks last week and they were just terrible. No change in the processing schedule, or the way Histogel was liquefied (placed in hot water that was heated in the microwave). Prior to the last two blocks, I must have processed at least a dozen blocks without any problems. There was an incident where I placed two histogels in the same cassete. One processed beautifuly and the other was all shrunken and dried up. I do not liquefy the entire tube, rather I scoop out the approximate amount that I need and transfer to another tube to heat up. If there is anyone out there in Histoland that has not had any issues with the Histogel, can you please post your procedure on liquefying the Histogel, method of cooling/solidifying and processing schedule? The only thing that I do that is not exact is I do not know the temp of my hot water when i place the Histogel to liquefy. I basically have to wait several minutes for the gel to melt and I use it immediately. Any new information or solution from anyone, would be greatly appreciated. Thank you Dusko Trajkovic From: Amy Porter port...@msu.edu To: Andrea Grantham algra...@email.arizona.edu; HISTONET histonet@lists.utsouthwestern.edu Sent: Mon, February 22, 2010 9:01:22 AM Subject: RE: [Histonet] DRGs I think it is strange that we are all doing similar techniques and wind up with different outcomes using the histogel. I would be curious how many of us are using the equipment sold with the histogel for warming and cooling opposed to any of us who don't. we did not purchase the equipment and I wonder sometimes if warming the histogel using other means causes some type of breakdown / and do any of you repeatedly reheat the same tube once it has been warmed and resolidified?? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Monday, February 22, 2010 9:41 AM To: HISTONET Subject: Re: [Histonet] DRGs Importance: High Hi Carol, I have used histogel for these kinds of samples and also other small, thin tissues like insect antennae and insect GI tracts and midguts. Since I get all my projects already fixed in whatever fixative the investigator chooses, rinsed and placed in 70% ETOH the histogel never touches formalin. I don't use formalin on my processor but start in 70%. I've never had a problem with the histogel. We just put the sample in the histogel flat and stand it up (turn 90°) when embedding in paraffin. I use tissue prep for embedding. If you don't want to use histogel you could try to put the drg's on GN Metricel membrane disc filters. We do this with a lot of the samples I receive, actually I have the investigators or their techs do this. The tissue sticks to the membrane and orientation is a dream. The membrane presents no problem when sectioning. You can get it from VWR. Andi Andrea Grantham, HT (ASCP) Senior Research
RE: [Histonet] Double labeling with antibodies that need different fixatives
Hi Phebe, In my experience that CD31 clone you refer to doesn't work well mainly b/c of paraffin embedding - in combination with PFA/formalin fixation. On PFA/formalin fixed frozen samples it works just fine with a trypsin antigen retrieval step. I find it's the paraffin that is the real killer. Try Biocare's anti-CD31, that works on FFPE. Or use another marker of endothelium (if that's what you are using CD31 for). Alternatively, are you sure your second antibody only works in FFPE formalin fixed tissue? Because it may well work on formalin fixed frozen tissue, then you can use both on that instead (just remember to do the trypsin digestion). Or use fresh snap frozen tissue postfixed in PFA for 10 minutes then no antigen retrieval necessary. Good luck! Andrea Hooper --- Phebe Verbrugghe pverbrug...@meddent.uwa.edu.au wrote: Hi all, I would like to do an immunofluorescent double labeling with two antibodies but 1 antibody works on acetone fixed frozen tissue but not on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370) and the other one works on formalin fixed paraffin embedded tissue but not on acetone fixed frozen tissue. Is there any way I could still do a double labeling and how? Also, does anyone have experience with zinc fixative? If my antibody works on formalin fixed tissue is it likely to also work on zinc fixed tissue? Thank you very much in advance, Phebe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QA Report
I was wondering if any one has special form that they provide to QA committee meeting in their hospital? This separate from all qc done in the lab!. Thanks,behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Any travel positions available?
Has anyone heard of any travel or contract positions? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Knife Holder CM1510
If anyone is in need of a CM 1510 leica cryostat knife holder for low profile blades, please contact me. Willing to part with this still functional item at a reasonable cost. FOR LOW PROFILE BLADES. Good price for back-up or replacement for Mohs or other enterprise. Not an easy item to find used. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
