[Histonet] TGF beta on FFPE

2010-03-08 Thread histonet . nospam
Hello Histonet,

I'd like to know if anyone of you has experience with TGF beta receptor and TGF 
beta ligand IHC on FFPE tissue.

Any input will be highly appreciated :)

Thanks a lot

V. Neubert





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[Histonet] Isopropanol monitring

2010-03-08 Thread S R

Good Monday Morning Everyone,

 

I was wondering if anybody out there that is microwave processing is monitoring 
for Isopropanol?

thanks in advanced

sammy
  
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[Histonet] Butyl alcohol

2010-03-08 Thread Krueger, Todd
Does anyone have a procedure for processing with N-butyl Alcohol. We
need to find a procedure w/o xylene.
Thanks
 
Todd Krueger

HTL(ASCP)CM

Boston Scientific

2 Scimed Place, P121

Osseo, MN 55311

Phone: 763-694-5709

Fax: 763-694-5505

e-mail: todd.krue...@bsci.com

 
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Re: [Histonet] Isopropanol monitring

2010-03-08 Thread Rene J Buesa
With a TWA of 400 ppm I don't think it is really a need for monitoring 
2-propanol.
René J.

--- On Mon, 3/8/10, S R sjkit...@live.com wrote:


From: S R sjkit...@live.com
Subject: [Histonet] Isopropanol monitring
To: histo net histonet@lists.utsouthwestern.edu
Date: Monday, March 8, 2010, 8:38 AM



Good Monday Morning Everyone,



I was wondering if anybody out there that is microwave processing is monitoring 
for Isopropanol?

thanks in advanced

sammy
              
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[Histonet] Embedding Forceps

2010-03-08 Thread kristen arvidson
Anyone use special forceps for embedding?  I have some recent concerns that 
some of the forceps out there may be to pointy/sharp for delicate tissue (ie. 
skin).  Any insight??



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RE: [Histonet] Embedding Forceps

2010-03-08 Thread Harlem Kaputnik


 Use the appropriate tool for the job needed... you don't want to damage the 
tissue.  For the pointy/sharp forceps, just use more of a delicate touch (this 
comes with practice)... they have the blunt tipped forceps that work great for 
skin.   
 Date: Mon, 8 Mar 2010 11:27:41 -0800
 From: arvidsonkris...@yahoo.com
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Embedding Forceps
 
 Anyone use special forceps for embedding?  I have some recent concerns that 
 some of the forceps out there may be to pointy/sharp for delicate tissue (ie. 
 skin).  Any insight??
 
 
 
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Re: [Histonet] Butyl alcohol

2010-03-08 Thread Rene J Buesa
Use 2-propanol instead. It is better and much cheaper.
René J.U

--- On Mon, 3/8/10, Krueger, Todd todd.krue...@bsci.com wrote:


From: Krueger, Todd todd.krue...@bsci.com
Subject: [Histonet] Butyl alcohol
To: histonet@lists.utsouthwestern.edu
Date: Monday, March 8, 2010, 10:00 AM


Does anyone have a procedure for processing with N-butyl Alcohol. We
need to find a procedure w/o xylene.
Thanks

Todd Krueger

HTL(ASCP)CM

Boston Scientific

2 Scimed Place, P121

Osseo, MN 55311

Phone: 763-694-5709

Fax: 763-694-5505

e-mail: todd.krue...@bsci.com


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re:[Histonet] TGF beta on FFPE

2010-03-08 Thread Hobbs, Carl

What species, V?
More info?
Them TGF ligand /receptors are as difficult as FGF/FGFr, imho.
respect,
Carlos.



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[Histonet] Positive control tissues for RANKL and/or OPG needed

2010-03-08 Thread Alexandra Meinl
Hello Histonetters,

Does anyone have experience with nice positive control tissues for RANKL
and/or OPG (except bone)?
Both are soluble factors, but is there a chance to produce a reasonably
discrete staining (of secretory vesicles?) in any organ?

Alexandra



Dr. Alexandra Meinl
Ludwig Boltzmann Institute
for Experimental and Clinical Traumatology
Austrian Cluster for Tissue Regeneration
Histology
Donaueschingenstrasse 13
1200 Vienna - Austria

Contact @ Bernhard Gottlieb University School of Dentistry,
Waehringerstr. 25a, A-1090 Vienna
tel:  +43 1 4277 67026
fax: +43 1 4277 67019
email: alexandra.me...@trauma.lbg.ac.at
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[Histonet] Comparison of Leica Peloris to Microm STP 420 D

2010-03-08 Thread Langenberg, Stacey
Can anyone give me some pros and cons on these 2 rapid processors?
Stacey Langenberg HT (ASCP) QIHC
CU Dermatopathology Consultants
12635 E. Montveiw Blvd. Suite 160
Aurora, CO 80045
(720) 859-3559

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[Histonet] Reminder: Deadline for hotel rates for Georgia meeting

2010-03-08 Thread Shirley A. Powell
Just a friendly reminder, the Evergreen Marriott Convention Resort is holding 
the discount rate for the Georgia Society for Histotechnology meeting (Stone 
Mountain, Ga, March 26-28th) until this Friday, March 12th.  After then, the 
rates go back to their normal rates.  Please make your reservations as soon as 
possible and definitely before Friday.

There is a link to the hotel on the GSH website, 
www.histosearch.com/gshhttp://www.histosearch.com/gsh, symposium page.  You 
will find the link just below the 2010 GSH program and the registration form 
links.  Click on the hotel link and it will take you directly to reservations 
and already has our meeting code entered.  All you do is finish the required 
information. Late fee for registering for the meeting will apply after March 
15th, so beat the deadlines.  Make your plans now and attend a great meeting.  
See you at Stone Mountain.

Also don't forget to celebrate National Histotechnology Professionals Day, 
March 10th, that is this Wednesday.  Have a party, bake a cake, take the day 
off, whatever, but do advertise it to your employer.

Shirley Powell
GSH Secretary

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[Histonet] (no subject)

2010-03-08 Thread Green JumpyOne
http://barowh.republika.pl/y7ZiEqxaqc.html
  
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[Histonet] (no subject)

2010-03-08 Thread Green JumpyOne
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Re: [Histonet] TGF beta on FFPE

2010-03-08 Thread Amos Brooks
Hi,
   If anyone has a good answer for this, please share it. I have been asked
to do this before and haven't had much confidence in the results.

Thanks,
Amos

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 Message: 1
 Date: Mon,  8 Mar 2010 11:29:49 +0100 (CET)
 From: histonet.nos...@vneubert.com
 Subject: [Histonet] TGF beta on FFPE
 To: histonet@lists.utsouthwestern.edu
 Message-ID: 20100308102949.afc24c299...@dd15630.kasserver.com
 Content-Type: text/plain; charset=utf-8

 Hello Histonet,

 I'd like to know if anyone of you has experience with TGF beta receptor and
 TGF beta ligand IHC on FFPE tissue.

 Any input will be highly appreciated :)

 Thanks a lot

 V. Neubert


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