[Histonet] Eosin too pink
Our Pathologists have, over the last few weeks, complained that the eosin is too pink. We have not altered our procedures or reagents in any way. It seems to be particularly bad in endoscopic biopsies. Thanks Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk http://www.ramsayhealth.co.uk/ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Surgical veterinary reference manual
Hi I need to locate a reference manual for veterinary surgical pathology - if anyone knows of one would you please contact me? Thanks Vikki Baker ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TGF beta on FFPE
Which receptor? We run BR1,BR2and BR3 on FFPE as well as B1,B2 B3. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Monday, March 08, 2010 8:13 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TGF beta on FFPE Hi, If anyone has a good answer for this, please share it. I have been asked to do this before and haven't had much confidence in the results. Thanks, Amos -- Message: 1 Date: Mon, 8 Mar 2010 11:29:49 +0100 (CET) From: histonet.nos...@vneubert.com Subject: [Histonet] TGF beta on FFPE To: histonet@lists.utsouthwestern.edu Message-ID: 20100308102949.afc24c299...@dd15630.kasserver.com Content-Type: text/plain; charset=utf-8 Hello Histonet, I'd like to know if anyone of you has experience with TGF beta receptor and TGF beta ligand IHC on FFPE tissue. Any input will be highly appreciated :) Thanks a lot V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Full Time Day Shift Position
At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura XPress 50 rapid tissue processor, Leica cryostats. Duties include embedding, microtomy, ihc and special stains, and frozen sections. DCH is a 500 plus bed regional medical center. We process around 12,000 to 13,000 specimens annually. Interested candidates please contact Sherrie Faulkner (recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stainers/Coverslippers
Just a quick thank you to all who responded to my inquiry about stainers/coverslippers...I am bringing all the info to the powers that be so we can start checking out some of these units. I appreciate all your help, Vicki Gauch AMCH Albany, NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Summer enployment
Hello, I am attending school at this momment, but this coming summer I am looking for part/full time work only for the summer. I am on my way to getting an associates so I can take the histology test. I am a Mohs technician (OHSU) almost 9 years, but was laid off last June, I also do histology (cutting and special stains). I am also interested in Clinical Reseach studies. If you are in need of summer help or tech relief email me and I will be glad to send you a resume. Best Regards, Robyn Vazquez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Region II Symposium
Histonetter's, Members of Region II and beyond.: Save the date for the 2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic City, N.J Come for a day. Come for the weekend. For more information about registration and accomodations contact Michele French: 609-818-3278 / michele.fre...@bms.com. Excellent Speakers, new workshops, vendor fair and of course...there is always the beach...(for studying, of course!). Stay tuned to the histonet, for more information regarding Seminars and Workshops. See you there. Carol Barone - Region II Director ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Surgical veterinary reference manual
A Google search for veterinary surgical pathology book brings up lots! John Kiernan Anatomy, UWO London, Canada. = = = - Original Message - From: Victoria Baker bakevicto...@gmail.com Date: Tuesday, March 9, 2010 9:53 Subject: [Histonet] Surgical veterinary reference manual To: histonet histo...@pathology.swmed.edu Hi I need to locate a reference manual for veterinary surgical pathology - if anyone knows of one would you please contact me? Thanks Vikki Baker ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Please remove me from your list. Thank you
Please remove me from your list. Thank you This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Helicobacter Pylori antibody
Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Helicobacter Pylori antibody
I have ordered mine from Cell Marque. We use it at 1/50 dilution with a 30 minute incubation at RT on the Dako Autostainers. Works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain [chak_...@yahoo.com] Sent: Tuesday, March 09, 2010 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Helicobacter Pylori antibody
Hi Chakib, We use Ventana's (manufactured by Cell Marque) H. Pylori on their instruments. The protocol is specific to the instruments but involves a routine HIER step and Avidin/Biotin blocking. The antibody itself is ready-to-use but is available from Cell Marque as a concentrate as well. The protocol produces a very clean stain; essential since these bugs are so small. Hope this helps, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain Sent: Tuesday, March 09, 2010 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Tek
I need the 2 parafin pots for a Tissue Tek/Sakura tissue processor working or not . Anyone has them ? Regards Ricky _ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/201469229/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Helicobacter Pylori antibody
We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20 minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare) 10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes hematoxylin(Dako). Works great. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain Sent: Tuesday, March 09, 2010 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Helicobacter Pylori antibody
We use Cell Marque's Polyclonal H. Pylori (CMC435) at a 1:100 dilution on the Ventana BenchMark for 32 minutes, Protease 1 for 12 minutes. ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks From: Chakib Boussahmain chak_...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Tue, March 9, 2010 11:45:54 AM Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin too pink
We add 100mL of 100% alcohol to 150mL of Eosin to tone it down each day. ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks From: Smith, Lesley lesley.sm...@ramsayhealth.co.uk To: histonet@lists.utsouthwestern.edu Sent: Tue, March 9, 2010 7:51:46 AM Subject: [Histonet] Eosin too pink Our Pathologists have, over the last few weeks, complained that the eosin is too pink. We have not altered our procedures or reagents in any way. It seems to be particularly bad in endoscopic biopsies. Thanks Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk http://www.ramsayhealth.co.uk/ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] hydrometer
We ordered ours from Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Region II mtg addiiton and up-date:
Histonetter's, Region II up-date: Save the date for the 2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic City, N.J ...Come for a day... or come for the weekend. Excellent Speakers, new workshops, vendor fair and of course...there is always the beach. ..(for studying, of course!). Stay tuned to the histonet, for more information regarding specific seminars and workshops. More to come... Information on registration will be posted on the histonet in April. So keep up with the histonet...for lots of great networking and info on the Region II Symposium See you there. Carol Barone - Region II Director...Oh!...and keep networking! It is the best educational tool of all! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p16
Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknut...@primecare.orgmailto:dknut...@primecare.org This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Butyl alcohol
As an additional note: paraffin oil = mineral oil. René J. --- On Tue, 3/9/10, Michael Folsom mwfol...@rgbio.com wrote: From: Michael Folsom mwfol...@rgbio.com Subject: Re: [Histonet] Butyl alcohol To: Krueger, Todd todd.krue...@bsci.com Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, March 9, 2010, 11:17 AM Hi: I'm not sure if this helps but Botanists have long used tert-butanol (not N-butanol) to embed tissue in paraffin. This classic protocol was popularized by D. A. Johansen in the mid 1930's and involves transition from water to alcohol to t-butanol (I can supply more detailed info if you need it). Once in 100% t-butanol tissue is transitioned to light paraffin oil. The final stage involves filling some large shell vials 1/3 full with paraffin and letting them harden. Tissue plus paraffin oil is added to the vials (volume of paraffin oil + tissue should be be approximately equal to the amount of paraffin). Vials are now placed in an oven for paraffin to melt and infiltration to begin. Besides doing enough changes of paraffin to infiltrate the tissue and remove all the paraffin oil that's about it. From there its the old embed, section and enjoy! Hope this helps - Mike Folsom Rio Grande Biological mwfol...@rgbio.com On Mon, 2010-03-08 at 09:00 -0600, Krueger, Todd wrote: Does anyone have a procedure for processing with N-butyl Alcohol. We need to find a procedure w/o xylene. Thanks Todd Krueger HTL(ASCP)CM Boston Scientific 2 Scimed Place, P121 Osseo, MN 55311 Phone: 763-694-5709 Fax: 763-694-5505 e-mail: todd.krue...@bsci.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Masson Trichrome, Alligator Heart Tissue, colors turning out purple
To Histonet: This is my first time mailing the histonet community. Coincidentally, this is my first venture into histology. My lab is conducting research on alligator heart tissue. At the moment, I am attempting to stain my slides using Masson Trichrome to differentiate muscle from connective tissue. Results have not been optimal. The slides turn out to be more purple than I had anticipated (comparing to pictures from journals). The red can be told apart from the teal, but the colors are not bright. Here is the procedure I am using: 1. Deparaffinize and hydrate to water 2. Iron Alum, 30 min 3. Wash in Dis. Water, 5 min 4. Hematoxylin stain, 30 min 5. Wash in Dis. Water, 5 min 6. Differentiate in saturated aq. picric acid, 1 min 7. Wash in dis. water, 10 min 8. Stain in acid fuschin, 5 min 9. Rinse in water 10. Stain in Ponceau de Xylidene, 5 min 11. Rinse in water 12. Differentiate in Phosphomolybdic acid, 5 min 13. Fast Green, 2 min 14. Differentiate in 1% aq. acetic acid, 5 min 15. Dehydrate 16. xylene (two washes, 3 min each) I would greatly appreciate any advice on how to modify my procedure to better stain the cardiac tissue. Or should I be using a different trichrome method better suited to this particular tissue? Thank you! -Colin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need help with FISH staining protocol on frozen tissue sections
Hi everyone, Has anyone done FISH staining on mice tissue frozen sections for Y chromosome? I need help with a successful protocol. Recently I started to do FISH staining on mice liver frozen sections for chromosome Y, but got no positive results even on male samples yet. The probe I used was IDMF1057 Green label Chr Y probe from ID Labs Inc. (www.idlabs.com). They have a protocol online probably for cultured cells (http://www.idlabs.com/product/datasheets/IDMF1057.pdf?PHPSESSID=b39f16507cf76b69495166c651f0b52c) which I haven't tried yet. I followed the following protocol for frozen sections: 1. Fix sections in 4% PFA at 4C for 10 mins, then wash in PBS. 2. Treat the sections with 0.01% Pepsin, 37C 10min, wash in PBS. 3. Dehydrate the slides using 70% Ethanol (2minsX2), 90% Ethanol (2mins X2), 100% Ethanol (5 mins X1).Then Age the slides at 65C for one hour. 4. Mix the probe and hybridization buffer. Denature the probe by incubate it at 65C for 10 mins, then hold it at 37C for 30 - 60 mins. 5. Denature the sections by incubate them in denaturing solution (formamide:2XSSC, 70:30) at 75C for 2 mins. 6. Quench the slides in 4C 70% Ethanol for 4 mins. Dehydrate the slides as step 3. 7. Apply the probe and hybridize at 37C overnight. 8. Immerse the slides in 1XSSC for 5 mins to remove the cover slips. 9. Wash slides in stringency solution (formamide:2XSSC, 50:50) at 45C, 5 mins X2. 10. Wash slides in 1XSSC at 45C, 5minsX2. 11. Wash slides in detergent wash solution at 45C, 5minX1. 12. Rinse slides in PBS. 13. Air dry for a couple of minutes and counterstain with DAPI. 14. Mount the slides and observe the results or keep in 4C for late check. Are there any problems with this procedure? Please help me to check, or if you could recommend a working protocol you had used, I would really appreciate. Thanks, Jinhui ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
