[Histonet] freezing mouse heart tissue
Hello, I am currently trying to produce cryosections from mouse heart tissue. I already have experience with paraffin-sections and had faced no major problems. But with cryosectioning I would ask for your help. You can get an idea of our current status at http://www.d-cup.at/histo/mouseheart.jpg this link (Hematoxylin test stain, not HE, the whole heart section looks like this) and as you might guess I am not satisfied with the quality. Would you call this freezing artifacts? Some people suggested that freezing with only LN2 would be not quick enough and create those ice crystals. Here is how we prepared the tissue: After taking out the hearts from the mice, we flushed them retrogradely via the aorta with cold sodium solution. Then we cut the hearts in half, put them into cryomolds and covered them with OCT. Afterwards they were snap-frozen in liquid nitrogen and stored at -80°C. Do you have an advice or maybe a suitable protocol for me? Would you recommend 2-methyl butane? Thank you very much! Greetings from the sunny Vienna! David -- Mit freundlichen Grüßen with kind regards Dr. David Santer Ludwig Boltzmann Cluster for Cardiovascular Research c/o Core Unit for Biomedical Research Waehringer Guertel 18-20 - Leitstelle 1Q A-1090 Vienna Austria Website: http://www.cardiovascular-research.at www.cardiovascular-research.at ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] freezing mouse heart tissue
Hi David, I wouldn't snap-freeze the tissue in the OCT blocks. For one this could cause the blocks to crack; you may have noticed this already in some of your blocks? But you may want to try freezing the OCT block by resting the block in a tray of isopentane (2-methylbutane) that has been frozen over liquid N, as you've already thought of. You can also search the Histonet archives on this topic as it's been discussed before here. Regards, Merced --On Friday, March 26, 2010 8:26 AM +0100 David Santer e...@gmx.at wrote: Hello, I am currently trying to produce cryosections from mouse heart tissue. I already have experience with paraffin-sections and had faced no major problems. But with cryosectioning I would ask for your help. You can get an idea of our current status at http://www.d-cup.at/histo/mouseheart.jpg this link (Hematoxylin test stain, not HE, the whole heart section looks like this) and as you might guess I am not satisfied with the quality. Would you call this freezing artifacts? Some people suggested that freezing with only LN2 would be not quick enough and create those ice crystals. Here is how we prepared the tissue: After taking out the hearts from the mice, we flushed them retrogradely via the aorta with cold sodium solution. Then we cut the hearts in half, put them into cryomolds and covered them with OCT. Afterwards they were snap-frozen in liquid nitrogen and stored at -80°C. Do you have an advice or maybe a suitable protocol for me? Would you recommend 2-methyl butane? Thank you very much! Greetings from the sunny Vienna! David -- Mit freundlichen Grüßen with kind regards Dr. David Santer Ludwig Boltzmann Cluster for Cardiovascular Research c/o Core Unit for Biomedical Research Waehringer Guertel 18-20 - Leitstelle 1Q A-1090 Vienna Austria Website: http://www.cardiovascular-research.at www.cardiovascular-research.at ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] freezing mouse heart tissue
You may found that excessive amount of saline could cause freezing artefacts, so dabe Try to embed heart sample on cryostat chuck directly, with OCT then freeze chuck on dry ice block directly as opposed to using liquid nitrogen. Another option, would be freezing tissue in hexane boiling tube, with Acetone/dry ice freezing mixture. Hope this help. Malika On 26 Mar 2010, at 13:22, Merced M Leiker wrote: Hi David, I wouldn't snap-freeze the tissue in the OCT blocks. For one this could cause the blocks to crack; you may have noticed this already in some of your blocks? But you may want to try freezing the OCT block by resting the block in a tray of isopentane (2-methylbutane) that has been frozen over liquid N, as you've already thought of. You can also search the Histonet archives on this topic as it's been discussed before here. Regards, Merced --On Friday, March 26, 2010 8:26 AM +0100 David Santer e...@gmx.at wrote: Hello, I am currently trying to produce cryosections from mouse heart tissue. I already have experience with paraffin-sections and had faced no major problems. But with cryosectioning I would ask for your help. You can get an idea of our current status at http://www.d-cup.at/histo/mouseheart.jpg this link (Hematoxylin test stain, not HE, the whole heart section looks like this) and as you might guess I am not satisfied with the quality. Would you call this freezing artifacts? Some people suggested that freezing with only LN2 would be not quick enough and create those ice crystals. Here is how we prepared the tissue: After taking out the hearts from the mice, we flushed them retrogradely via the aorta with cold sodium solution. Then we cut the hearts in half, put them into cryomolds and covered them with OCT. Afterwards they were snap-frozen in liquid nitrogen and stored at -80°C. Do you have an advice or maybe a suitable protocol for me? Would you recommend 2-methyl butane? Thank you very much! Greetings from the sunny Vienna! David -- Mit freundlichen Grüßen with kind regards Dr. David Santer Ludwig Boltzmann Cluster for Cardiovascular Research c/o Core Unit for Biomedical Research Waehringer Guertel 18-20 - Leitstelle 1Q A-1090 Vienna Austria Website: http://www.cardiovascular-research.at www.cardiovascular-research.at ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] elisa's
Is there a list serve for elisa's? Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslippers
Yes, I too would like to know if any one has ever used The Dako coverslipper and if they liked it or not. Debora Probst HT Columbus Regional Columbus, Ga. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Question
How often are people changing/rotating their paraffin? In other words the dirtiest paraffin is how many days old? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Question
This will depend on the amount of blocks processed. I worked in places who used to change their VIP twice a week on Wednesday Friday, and other that would change processor solutions once a week, but as a rule, processor wax regardless of the make should ALWAYS be changed after a maximum of 5 run for optimal result. Cheers, Malika My current lab On 26 Mar 2010, at 14:57, kristen arvidson wrote: How often are people changing/rotating their paraffin? In other words the dirtiest paraffin is how many days old? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Question
I have my processor set to notify me at 1500 blocks, at which time I change the paraffins. I usually run 500 in the other solutions (same notification system) before changing. It works for me! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FFPE used for EM
Hi Everyone, I hope it's a great Friday for you all. Our renal folks were recently attending a meeting, and they came back with some partial information. (I am hoping some of you might have some additional information.) It seems that one of the lecturers mentioned that some paraffins actually seemed to make a difference in the quality of EM when they had to take tissue from the paraffin blocks. I had never heard of this. I am aware of using Carson's modification of formalin to yield better EM results, but not anything to do with paraffin. Can anyone help me out here? Thanks! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PA baby sitter
That's OK, our poor PA has to babysit the residents, so she needs all the help she can get!! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Tue 3/23/2010 5:24 PM To: rick.garnh...@memorialhealthsystem.com; Jeffrey Silverman Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PA baby sitter Tell be about it again when you get to gross 38,000 cases/year. René J, --- On Tue, 3/23/10, Jeffrey Silverman pathmas...@yahoo.com wrote: From: Jeffrey Silverman pathmas...@yahoo.com Subject: [Histonet] PA baby sitter To: rick.garnh...@memorialhealthsystem.com Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, March 23, 2010, 5:18 PM I had to laugh when I read this one. I'm a PA grossing 8500 surgicals per year in a busy general hospital so it's not just biopsies but lots of major organ resections with tons of orthopedics. I work with two histotechnologists. Not only do I close my own cassettes, I accession most specimens, make my own cassettes, save and dispose of the surgical leftover tissues, serve as histology supervisor and laboratory safety officer for all sections attending all the associated meetings that those two entail, often embed at least half of the tissues and pitch in whenever I'm needed in histo- cutting and running automated specials. Having said that, if there's anyone in Europe looking for such a person and can pay 60K euro per annum , I'd love to meet you. I'm in the opposite boat of Malika. Hey, wanna trade jobs? The techs are at my beck and call to bring me things I need, like more acetone or formalin and/or to attend to whatever other help I need, but no one ever sits with me to close the cassettes and feed me specimens. I agree with the accountability issues involved in lost or mishandled tissue. What happens when the aide is off, do they expect a histotech to come in to close the cassettes for the PA. And the productivity increase for such assistance can't be more than 5% IMHO based on my experience. Jeff Silverman ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] freezing mouse heart tissue
Wow, a whole book has been written on frozen sections? That's fantastic! I've got to get a hold of that! --On Friday, March 26, 2010 8:45 AM -0500 charles.scou...@leica-microsystems.com wrote: There is a thorough discussion of these issues in Dr. Peters Book, A Practical Guide to Frozen Sections Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Malika Benatti malbena...@googlemail.com Sent: Friday, March 26, 2010 8:33 AM To: Merced M Leiker lei...@buffalo.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] freezing mouse heart tissue You may found that excessive amount of saline could cause freezing artefacts, so dabe Try to embed heart sample on cryostat chuck directly, with OCT then freeze chuck on dry ice block directly as opposed to using liquid nitrogen. Another option, would be freezing tissue in hexane boiling tube, with Acetone/dry ice freezing mixture. Hope this help. Malika On 26 Mar 2010, at 13:22, Merced M Leiker wrote: Hi David, I wouldn't snap-freeze the tissue in the OCT blocks. For one this could cause the blocks to crack; you may have noticed this already in some of your blocks? But you may want to try freezing the OCT block by resting the block in a tray of isopentane (2-methylbutane) that has been frozen over liquid N, as you've already thought of. You can also search the Histonet archives on this topic as it's been discussed before here. Regards, Merced --On Friday, March 26, 2010 8:26 AM +0100 David Santer e...@gmx.at wrote: Hello, I am currently trying to produce cryosections from mouse heart tissue. I already have experience with paraffin-sections and had faced no major problems. But with cryosectioning I would ask for your help. You can get an idea of our current status at http://www.d-cup.at/histo/mouseheart.jpg this link (Hematoxylin test stain, not HE, the whole heart section looks like this) and as you might guess I am not satisfied with the quality. Would you call this freezing artifacts? Some people suggested that freezing with only LN2 would be not quick enough and create those ice crystals. Here is how we prepared the tissue: After taking out the hearts from the mice, we flushed them retrogradely via the aorta with cold sodium solution. Then we cut the hearts in half, put them into cryomolds and covered them with OCT. Afterwards they were snap-frozen in liquid nitrogen and stored at -80?C. Do you have an advice or maybe a suitable protocol for me? Would you recommend 2-methyl butane? Thank you very much! Greetings from the sunny Vienna! David -- Mit freundlichen Gr??en with kind regards Dr. David Santer Ludwig Boltzmann Cluster for Cardiovascular Research c/o Core Unit for Biomedical Research Waehringer Guertel 18-20 - Leitstelle 1Q A-1090 Vienna Austria Website: http://www.cardiovascular-research.at www.cardiovascular-research.at ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New
Re: [Histonet] Paraffin Question
You cannot measure paraffin changes by dates because it is used as a function of the number of cassettes processed. I always used VIP that have 4 paraffin containers. I used to keep track of the number of cassettes processed daily. When I got to as many cassettes as the VIP was designed to (e.g.: 300 cassettes for VIP 300) I discarded the first paraffin, moved forward #s 2 and 3, and added new paraffin in #4. By dpoing this the #1 was discarded when the VIP had processed 900 cassettes. René J. --- On Fri, 3/26/10, kristen arvidson arvidsonkris...@yahoo.com wrote: From: kristen arvidson arvidsonkris...@yahoo.com Subject: [Histonet] Paraffin Question To: histonet histonet@lists.utsouthwestern.edu Date: Friday, March 26, 2010, 10:57 AM How often are people changing/rotating their paraffin? In other words the dirtiest paraffin is how many days old? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] California Histology Society
Hi There, Anyone out in histoland know why there's no access to www.californiahistology.org ? I've tried a few times this morning with no luck. I want to send one of my techs to the symposium and need to access the event site. Thanks in advance for any help or explanation. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Coverslippers
For those who are not aware, the Dako unit is apparently the same coverslipper previously marketed by Surgipath. This information may lead to greater responses since Dako has only recently acquired this unit as I understand it. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debora Probst Sent: Friday, March 26, 2010 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslippers Yes, I too would like to know if any one has ever used The Dako coverslipper and if they liked it or not. Debora Probst HT Columbus Regional Columbus, Ga. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Question
I was instructed by a very knowledgeable person in the field that the best processing for your tissue is to change it as often as you change your clearant. So, if you change your clearant after 5 uses, you should also change the parafffin after 5 uses too. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 kristen arvidson arvidsonkris...@yahoo.com 3/26/2010 10:57 AM How often are people changing/rotating their paraffin? In other words the dirtiest paraffin is how many days old? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] California Histology Society
The server was down. You can also access the site through www.californiahistology.com I can also email you a PDF file of the program Jennifer Thomas Jasper tjas...@copc.net Sent by: histonet-boun...@lists.utsouthwestern.edu 03/26/2010 08:32 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] California Histology Society Hi There, Anyone out in histoland know why there's no access to www.californiahistology.org ? I've tried a few times this morning with no luck. I want to send one of my techs to the symposium and need to access the event site. Thanks in advance for any help or explanation. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Coverslippers
I saw it at the NSH meeting in Alabama and have been trying to get one ever since. Although I have never had the chance to use it in my lab. It's very small and fast. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie [del...@musc.edu] Sent: Friday, March 26, 2010 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Coverslippers For those who are not aware, the Dako unit is apparently the same coverslipper previously marketed by Surgipath. This information may lead to greater responses since Dako has only recently acquired this unit as I understand it. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debora Probst Sent: Friday, March 26, 2010 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslippers Yes, I too would like to know if any one has ever used The Dako coverslipper and if they liked it or not. Debora Probst HT Columbus Regional Columbus, Ga. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histo techs doing cyto prep work
Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? I welcome any and all responses... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] looking for employment
I am inquiring about employment for my oldest daughter. She is looking for a part time 20 + hours lab assistant position in the Omaha area. She is relocating from CA. She is working on her BSRN and can be flexible with her schedule. The earlier the hours the better. She has some knowledge of Histology and a good work ethic. Thanks Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] histo techs doing cyto prep work
In our lab we (Histo Techs) do the cyto prep work and our supervisor is a cyto tech and HTL she covers both Dept. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, March 26, 2010 12:43 PM To: Histonet Subject: [Histonet] histo techs doing cyto prep work Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? I welcome any and all responses... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histo techs doing cyto prep work
Cytology preparation was added to the HT (ASCP) exam back in 2001 based on feedback on the number of laboratories that have histotechnicians doing cytology preparation. It was also added as a new chapter in the Carson/Hladik Histotechnology text book. Jennifer MacDonald Kim Tournear kimtourn...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 03/26/2010 09:45 AM To Histonet histonet@lists.utsouthwestern.edu cc Subject [Histonet] histo techs doing cyto prep work Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? I welcome any and all responses... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histo techs doing cyto prep work
Working in Pediatric, without a cytology department, I get opportunity to handle non-gynae cytology sample such as Bronchial Alveolar Lavage, CSF, urine, cyst aspirate blood sample, though all the sample are handle by Registered Biomedical Scientist that have been trained to handle cytological specimen. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH United Kingdom Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 On 26 Mar 2010, at 16:42, Kim Tournear wrote: Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? I welcome any and all responses... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histo techs doing cyto prep work
I have worked in union hospital for almost 39 yrs. Back then, there were no cytotechs and the pathologists read out all the cytology slides. As a result, the cytoprep was in our contract to perform. Years later, when we did get cytotechs, they preferred to do all of their prep. 10 years later, they are so swamped, that we are going to take the prep back. As it is in our contract to still perform this task, there is no choice there. In my opinion, ideally, a prep tech should be hired instead. Histotechnology has greatly evolved over the years, and much more is required of us, such as IHC, ISH and more. While it is always good to know these procedures so you can step in if the need arises, a prep tech is a better business choice. In my opinion.!! Happy Friday, Lynette Kim Tournear kimtourn...@yahoo.com 3/26/2010 12:42 PM Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? I welcome any and all responses... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 76, Issue 40
Kim- Our cytoprep area is in with Histology so we are currently training new people coming into the department to do both. We feel it will give us more flexibility with scheduling and just being able to help each other out. I too would be interested in hearing what others are doing. Nancy Schmitt HT(ASCP), MLT(CSMLS) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext.142 nancy_schm...@pa-ucl.com -- Message: 8 Date: Fri, 26 Mar 2010 09:42:38 -0700 (PDT) From: Kim Tournear kimtourn...@yahoo.com Subject: [Histonet] histo techs doing cyto prep work To: Histonet histonet@lists.utsouthwestern.edu Message-ID: 561894.62023...@web54207.mail.re2.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? ? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? ? I welcome any and all responses... ~Kim Tournear?~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,? AZ ~Don't?let your life end before it begins~ OU Rocks NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] looking for employment
Have you checked NSH.org for job postings How about MOHS? They are all short of good techs. Not sure what NE's regs are for Mohs tech requirements but some states I see MT, LPN, RNs do it You can find all mohs docs by state and inquire with each office if they need help http://www.mohssurgery.org/i4a/member_directory/feResultsListing.cfm?dir ectory_id=3 or try http://acms.execinc.com/edibo/SurgeonFinder -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, March 26, 2010 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for employment I am inquiring about employment for my oldest daughter. She is looking for a part time 20 + hours lab assistant position in the Omaha area. She is relocating from CA. She is working on her BSRN and can be flexible with her schedule. The earlier the hours the better. She has some knowledge of Histology and a good work ethic. Thanks Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] histo techs doing cyto prep work
That is how I started many moons ago(29 years and counting). I spent 1 week in histology, the next week in cytology, this continued for 2 years. I currently supervise both the histology and the cytology lab sections. My histotechs do little cytology prep work but are willing if time allows to help out at the end of the day. The cyto prep techs are willing to learn histology but there is little time to take advantage of their willingness. Hope this answers your question. Happy Friday Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, March 26, 2010 12:43 PM To: Histonet Subject: [Histonet] histo techs doing cyto prep work Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? I welcome any and all responses... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] embedding method for DRGs
Histonetters- Back to these DRGs again: We are looking for a better way to embedd DRGs from neo-natal mice, for cryotomy.. We normally...using a dissecting scope, remove the DRG from the eppendorph tube with a small spatula from ...touch the drg to some OCT (colored) and then touch the OCT to the bottom of a disposable embedding mold (peel-away)...the DRG releases and we fill the mold and move on. But many times we need to encourage these neo-natal DRGs off the spatula, with another spatula to get the DRG to release into the OCT. Though this technique works...it is a very time consuming method - and we occassionally do lose one or two of these things, because they are so very very small. We are using marker dye to help us locate after they are on the spatula and getting them from the tube to the spautla AOK, it is getting them from spatula to OCT that is the killer. We are currently drawling off the dye with the point of a Kimwipe and touching the DRG to a frozen dot of OCT, using the cold to capture and hold the DRG on the dot, before finishing the embedding process. This is working slightly more efficiently, but Does anyone have a better technique to share? I am losing tech's to DRG blindness!...It's like trying to embedd a speck of dust...and everyone hates to do them. This is the only technique I have ever usedbut always on full term mice..No problem. It works great; but on the neo-natal micewell you all get the picture. Too teeny tiny.frustrating, and takes a two man team to do! Ever so time consuming to do! PS: histo-gel doesn't not work, either. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech position in Little Rock
Hi Everyone, I hear that there is a histotech position open at University of Arkansas Medical Center. If you are interested, go online and apply! There are some great folks in Arkansas! I'm sure it will be a great opportunity for the right person. Bonnie Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] coverslipper
Hacker Instruments is still the go-to company for new or used RCM and HCM style coverslippers. Dorothy In a message dated 3/25/2010 3:51:13 P.M. Eastern Daylight Time, flna...@texaschildrens.org writes: Is this coverslipper now produced by Medite -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Thursday, March 25, 2010 2:40 PM To: malbena...@gmail.com; 'Lynette Pavelich' Cc: histonet@lists.utsouthwestern.edu; 'Naira Margaryan' Subject: RE: [Histonet] coverslipper I purchased a used Hacker RCM-3660 glass coverslipper several years ago. It was missing some parts and the nice people from Hacker supplied me with the needed parts. This work horse has been running 7 days a week for the past several years and has never given us any problems. Great machine and great people. Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Thursday, March 25, 2010 3:08 PM To: Lynette Pavelich Cc: histonet@lists.utsouthwestern.edu; Naira Margaryan Subject: Re: [Histonet] coverslipper We use the Leica CV5030 the advantage of it is that it can be attached to their Auto stainer providing continuous workflow as rack and coverslip 30 slides per run, without the need to physically transfer slides rack between the autostainer and coverslipper or used as a stand alone coverslipper if needed, though at time it can be problematic. For a start glass cover slip must be kept at 37 oC prior use otherwise they tend to stick to each others. Also does not recognised all the slides type unless they are Leica one, or the coverlslipper as been calibrated to use a specific slides type. In the past I used the Sakura Acetate film coverslipper, and their were no major problem with it though remounting section was a very tedious process. Malika ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
