RE: [Histonet] Slide and Block Storage
If you are supplying the professional component, then it is a good idea to maintain the blocks and slides. If you are inspected and they do an audit trail you must be able to produce the slides and/or blocks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Thursday, April 29, 2010 11:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide and Block Storage
I am able to reply to questions n the histonet but I am unable to post anything on the histonet. Is there a different address I should be using instead of histo...@list.utsouthwestern.edu? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, April 30, 2010 7:12 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If you are supplying the professional component, then it is a good idea to maintain the blocks and slides. If you are inspected and they do an audit trail you must be able to produce the slides and/or blocks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Thursday, April 29, 2010 11:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] MAILING BIOPSIES
Hi All, There has been quite a large response to the shipping regulations of mailing specimens, as I would expect to the guidelines changing consistently. I will be at the CLMA show in Vegas on May 4th and 5th. Please feel free to stop by at booth #626 and I will be available to answer more questions regarding the regulations and make suggestions for you. I will also have samples of different specimen mailing kits. You can bring me (or mail me) your current kits and we will evaluate them for free, letting you know if they meet regulations and if other improvements can be made. Thanks, Canyon Bowie Path-Tec 3151 Williams Rd Bldg C Columbus, GA w. 706.507.1575 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Thursday, April 29, 2010 8:19 AM To: histonet Subject: [Histonet] MAILING BIOPSIES HISTONETTERS I was wondering if there are any REGS out =here about mailing formalin filled biopsies thru UPS or FED EX other than =he special package and the spill pad. Anyone have any knowledge about the subject? Thanks Pathology Supervisor Kathy Ba=dwin, SCT (ASCP) Memorial Hospital and Health=bsp;Care Center [1]sbald...@mhhcc.org Ph 812-482-0210, 482-0216, nbs=;Fax 812-482-0232, Pager 812-481-0897 =R Confidential information, Authorized use only. References 1. 3Dmailto:sbald...@mhhcc.org; ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide and Block Storage
If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide and Block Storage
So what if the reporting company need to order special stains, it adds too much time having to request the blocks or additional unstained slides. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 9:40 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide and Block Storage
Clients fax orders for deepers and special stains which we perform and send them out with their next package for them to read and report. -Original Message- From: Nails, Felton [mailto:flna...@texaschildrens.org] Sent: Friday, April 30, 2010 10:48 AM To: 'Elizabeth Kronenberger'; 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage So what if the reporting company need to order special stains, it adds too much time having to request the blocks or additional unstained slides. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Kronenberger Sent: Friday, April 30, 2010 9:40 AM To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block Storage If our lab does slide preps for other clients (Technical component only), we hold the blocks at our facility. The slides are read and reported by the client, they hold the slides. Liz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Adams Sent: Friday, April 30, 2010 12:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage My laboratory provides professional services to some clients and technical services to others. The question has recently come up, who should store blocks and slides when the pathologist and histology laboratory are different companies? - Dan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68
Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68
Hi Cindy, My lab uses KP-1. Mark On Fri, Apr 30, 2010 at 8:48 AM, Cynthia Pyse cp...@x-celllab.com wrote: Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68
We also use KP1 Sally -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject: [Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Repeat validation when IHC protocol changed?
Recently our IHC vendor sent a tech to help us out with some weak staining affecting multiple antibodies. Several staining protocols were changed, including adjusting the incubation time and adding amplification. The results were great in the slides that I was shown. The tech said that revalidation was unnecessary, since the changes to the protocol were considered minor. However, I personally disagree, since we don't know how the change in the staining protocol could potentially affect staining of other tissues (especially tissues that would be expected to be negative). Before subjecting our lab to the cost and effort of revalidation, I'd like to get your opinion. For new antibodies, I usually validate using 10 cases expected to be positive and 10 cases expected to be negative for the antibody in question. For revalidation, I was thinking of running 5 positive cases and 5 negative cases. Thanks for your input - Kevin Kurtz, M.D. St. Mary's Hospital Madison, Wisconsin Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How to troubleshoot a cytology slide with dehydration problems
I am an HTL but I have to stain pap smears. In the Papanicalou procedure for fine needle aspirations I have to stain the slides in sodium chloride first for 25 seconds in total (15 seconds in one container and 10 in another container). This for me is the trickiest part when staining cytology because if slides are not well dehydrated in saline then the cells are not going to absorb the stains well, and the result will be a pinkish slide, when it is suppose to look blue-greenish. I had this problem today where I stained an entire rack of slides, and all the slides turned blue (which means they stained correctly) except 4 slides which turned out pinkish (which is a sign that they did not dehydrated properly in sodium chloride (h20 saline). Is there a solution to this issue. Can these slides be re-stained? I know this newsgroup is for histology only but I would like to know if histotech and cytotechs have encountered this issue in their labs and how they ended up solving the problem. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: How to troubleshoot a cytology slide with dehydration problems
I forgot to include the complete protocol that my lab uses so you can help me better: 1.After dehydrating in sodium chloride for 25 seconds you have to dip the slides in alcohol 95. 2. 10 dips in water then 1.30 minutes in hematoxylin, it used to be 1 min, then rinse in water. 3. 1 min in rinse solution 4.10 dips in water. 5. 1 min in bluing solution 6. 10 dips in water. 7. 10 dips in alcohol. 8. 10 dips in alcohol. 9. 1 min in orange solution 10. 15 dips in 95 alcohol. 11. 15 dips in 95 alcohol. 12. 3.15 mins in EA solution 13. The slides are then dipped in 3 containers of 95 alcohol (20 dips each) 14. Slides are dipped in 3 containers of 99 alcohol (15 dips in each) 15. Then they are dipped in Isoxylene which is a combination of xylene and 99 alcohol (15 dips) 16. And lastly Xylene 10 dips When the slides do not absorb the h20 saline seems that the slides absorb more EA than hematoxylin. I don't know why. On Fri, Apr 30, 2010 at 5:34 PM, Valerie R. vrodrigue...@gmail.com wrote: I am an HTL but I have to stain pap smears. In the Papanicalou procedure for fine needle aspirations I have to stain the slides in sodium chloride first for 25 seconds in total (15 seconds in one container and 10 in another container). This for me is the trickiest part when staining cytology because if slides are not well dehydrated in saline then the cells are not going to absorb the stains well, and the result will be a pinkish slide, when it is suppose to look blue-greenish. I had this problem today where I stained an entire rack of slides, and all the slides turned blue (which means they stained correctly) except 4 slides which turned out pinkish (which is a sign that they did not dehydrated properly in sodium chloride (h20 saline). Is there a solution to this issue. Can these slides be re-stained? I know this newsgroup is for histology only but I would like to know if histotech and cytotechs have encountered this issue in their labs and how they ended up solving the problem. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
