[Histonet] Formalin and Kylen recycling
Some help Has anyone else use use BP instruments for� recycling. I need some information about the subject. Marinez Barra Laboratory of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] waterbath residue
Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] grossing qualifications
I was asked to get opinions about grossing personnel. We have a tech with an Associate of Science in Medical Assistant with 8 credits in Anatomy and Physiology, 4 credits in Microbiology, 3 credits in Medical Terminology, and 16 credits in Medical Assistant Theory and Practicum. Would you consider her qualified to gross specimens? Thanks, Donna This message has been scanned for malware by Websense. www.websense.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] waterbath residue
Are you using pencils to mark your slides? Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of path lab Sent: Tuesday, May 04, 2010 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] waterbath residue Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CBG BIOTECH RECYCLER
Nirmala We have the CBG recycler also. We initially started recycling both alcohol and xylene, but we now only recycle alcohol. We ended up with so much recycled xylene that we could not use. Also you can not use the recycled xylene on the last xylene station in the tissue processor, or for cleaning. It just did not work out for us for the xylene. We rotate our xylenes and alcohols weekly so we are always putting on one fresh absolute and one fresh xylene, there was never a place for us to use the recycled xylene, so we ended up with lots of it. If you change your entire tissue processor then you could put a recycled xylene in place of your first xylene and then use fresh xylene for the last station. We do recycle the alcohol. We get about 95% alcohol out of the recycler - we test it for percentage of alcohol via a hydrometer and for contamination with xylene by adding water to a small portion of it. We only use it for 95% or less so we make up our 50%, 70% and 80% alcohol from the recycled alcohol, these solutions do go on our tissue processor and in the staining set up and they seem to work just fine. To be honest we do not keep track of how many times the alcohol has been recycled we just keep recycling it. As for disposal we have a really cool flammable cabinet that houses a 55 gallon drum, the drum is on rollers so it can be moved easily. All of our waste goes in there its picked up whenever it is full. We use Source/AET Environmental to dispose of all our liquid waste. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Tuesday, May 04, 2010 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CBG BIOTECH RECYCLER Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] waterbath residue
I used to glue a black X-ray film separating paraffinized sheet to the bottom of the water bath for a black background and changed it as needed. René J. --- On Tue, 5/4/10, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: Re: [Histonet] waterbath residue To: path lab pathology.histol...@gmail.com, histonet@lists.utsouthwestern.edu Date: Tuesday, May 4, 2010, 11:56 AM Those black flecks could absolutely be from the waterbaths. I have yet to find a coated waterbath that does not eventually loose its lining. In frustration, I've had techs use a SOS pad to scrub off the lining to finally get rid of it. Waterbath still worked, just no nice teflon lining. I've tried returning them, but they all still went bad. Maybe next purchase, you could go the glass liner type. path lab pathology.histol...@gmail.com 5/4/2010 10:56 AM Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Lynn Burton, Galesburg, IL
Hello Lynn Burton of Animal Disease Lab, Galesburg, IL will you please contact me ASAP regarding a 2006 Histonet posting. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histochoice fixation
I wish to test how the Histochoice ( Amresco) fixative works in lieu of 4% paraformaldehyde for my frozen brain sections in order to avoid the subsequent antigen retrieval step. Can anybody share their experience how it works ? For how much time should I use it for fixation? Thanks Ayanabha Chakraborti, Ph.D Dept of Neurosurgery University of California, San Francisco. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] For Sale Used Leica EG1160
I have a Leica EG1160, 5.5 yrs old, dispenser is the only thing that does not work. -Paul --- To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: CBG BIOTECH RECYCLER
We use the CBG Biotech Recycler to recycle formalin, alcohol and xylene and they all turn out very nicely. We always assay/test each batch to make sure it is ok to use. The xylene always comes out at 99.9% and we use it on all stations of our processor and to stain with and have no problems. We collect our waste alcohols as batches of 70% and 80% to be recycled together and then 95% and 100% to be recycled together. Ususally we get back between 83% and 98% from the reclaimed alcohol and dilute accordingly. We were told by CBG Biotech Tech Support, there is no limit to how many times you can recycle any of the products. Also, you can recycle xylene with paraffin in it, but DO NOT recycle any alcohol contaminated with xylene. We do not recycle any alcohol off the stainer or the cleaning alcohol off the processors. The waste we end up with that cannot be recycled goes in the 55 gal. barrels to be hauled off for proper disposal. Hope this helps! Susan Bincsik, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
