RE: [Histonet] fume hood
Hi Brandy, I have recently had the opportunity to build a Path lab from scratch. In the design we decided to completely separate the grossing area from the microtomy and IHC area of the lab. We built a room within a room, made it negative pressure, installed 2 Thermo elevating grossing stations that are vented to the outside. Since we are using the hospital ventilation system in addition to the blowers built in to the back draft, downdraft capabilities of the grossing stations, we were able to set these to pull at 500 cfm each. We also put 2 Peloris processors, with their own charcoal filters, within this room. The result is that we are well under the limits for all fumes and, in the event we get fresh tissue, we can segregate the area from the rest of the lab. Many labs that have to do autopsy on babies or near full term fetus' use their grossing stations to do so. Since we are in a separate area, we can block these procedures from view. We also put in a Labconco Fume hood (vented to the outside) in the IHC area of the lab and a Thermo Bio Hood in the cytoprep area. This has all worked out very well for us and it affords us the opportunities to have these items in place for future growth. A renovation done correctly, with an eye towards strategic planning for the future, will go a long way towards saving the hospital money in the long run. Steve Feher Pathology Supervisor Catholic Medical Center Manchester, NH -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Tuesday, June 15, 2010 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fume hood Hello, Our hospital is doing some renovation and we need to look into new fume hoods for our new location. Currently we have one fume hood over our grossing area, and one fume hood in our coverslipping area (two different rooms). The hospital wants to put our grossing room and histo/cyto rooms together. I am still going to need two separate hoods. Does anyone have any experience/knowledge/input about fume hoods? I'm trying to look into the ductless ones, although I imagine changing the filters will end up being more expensive over time (I have no idea what would be involved in running a duct/vent). Also I have seen a benchtop downdraft type that sucks the air down, and does not have a top. It is advertised as being good for xylene. Does anyone use this in their coverslipping area? Any input would be greatly appreciated. I'm pretty clueless on the whole issue. I want to make sure that what I get will be safe for me and my coworker as we will be spending most of our day in this room. Any input is appreciated! Thank You! Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thermo Slide Mate Print Mate
Is anyone using the Thermo Slide Mate Print Mate with CoPath as your AP LIS? I am trying to decide if I am going to look at this as a stand alone process or integrate it with my AP LIS. I would like to hear from those that use it both ways and if it is okay to contact you off line. Thanks, Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thermo Slide Mate Print Mate
Mike, We have the Print Mate and 5 Slide Mates. We are not connected to our LIS - we use it as a stand alone process. Everything is set up very basic and the system works very well for us. Feel free to contact me offline. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, June 17, 2010 6:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Slide Mate Print Mate Is anyone using the Thermo Slide Mate Print Mate with CoPath as your AP LIS? I am trying to decide if I am going to look at this as a stand alone process or integrate it with my AP LIS. I would like to hear from those that use it both ways and if it is okay to contact you off line. Thanks, Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] how to prevent foldings on femoral head cartilage tissue
Hello histonetters especailly hard tissue group I have a pig femoral head bone tissue embedded in paraffin and I have a hard time getting rid of the folding problem. I tried to remedy by lowering my temperature to 38 C and putting them in 5% alcohol before placing them in water bath I still have a lots of folding formation on some areas of the cartilage. Is there any other technique to remedy this problem. I appreciate your help. Thank you. reuel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] how to prevent foldings on femoral head cartilage tissue
Sometimes placing them on a hot plate at about 60C will help get out the folds, the paraffin needs to melt and the sections need to turn clear then take it off the hot plate. If you leave it too long on the hotplate the articular cartilage may fold over on itself. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: Thursday, June 17, 2010 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to prevent foldings on femoral head cartilage tissue Hello histonetters especailly hard tissue group I have a pig femoral head bone tissue embedded in paraffin and I have a hard time getting rid of the folding problem. I tried to remedy by lowering my temperature to 38 C and putting them in 5% alcohol before placing them in water bath I still have a lots of folding formation on some areas of the cartilage. Is there any other technique to remedy this problem. I appreciate your help. Thank you. reuel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Breast Specimen handling
Sara, Funny that this comes up now. I attended a meeting last week and this was one of the topics. It was suggested that the tissue be stored in 70% alcohol. I personally have not done this but I am going to start doing this. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Sara Baldwin/mhhcc.org To sbald...@mhhcc.o Histo Net rg histonet@lists.utsouthwestern.edu Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Breast Specimen handling 06/17/2010 01:24 PM Hi histonetters Was wondering if anyone saw the new gui= delines for breast Cancer specimen handling ? I am sure you have.nbs= p; What my Pathologist is wondering is if you have a large specimen and do = not use all the tissue for processing what would the agent be to apply= to the specimen to stop penetration of the formlalin before the 72 hour ti me arrives? Would it be sodium chloride? Can someone help? = /DIV Thanks Pathology Supervisor Kathy Baldwin, S= CT (ASCP) Memorial Hospital and Health Carenbs= p;Center [1]sbald...@m= hhcc.org Ph 812-482-0210, 482-0216, Fax 81= 2-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. References 1. 3Dmailto:sbald...@mhhcc.org; ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] how to prevent foldings on femoral head cartilage tissue
Reuel, Do the opposite and turn up the temp on the waterbath and let the section float out a little before the wax starts to break up. You can even gently use the forcepts to tease out any folds and this will definitely help to release any wrinkles in the cartilage. Then, make sure the section is free of large droplets of water before transfer of the slide to the slide warmer. Next, let slide sit on slide warmer until the wax is melted and then bake the section for a few minutes at a higher temperature to firmly secure the section to the glass slide. Bob Skinner at UAMS showed me this technique at the beginning of the year and it works nicely, especially for even larger human femoral heads! Jack Date: Thu, 17 Jun 2010 12:10:10 -0500 From: reuel.corne...@tsrh.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to prevent foldings on femoral head cartilage tissue Hello histonetters especailly hard tissue group I have a pig femoral head bone tissue embedded in paraffin and I have a hard time getting rid of the folding problem. I tried to remedy by lowering my temperature to 38 C and putting them in 5% alcohol before placing them in water bath I still have a lots of folding formation on some areas of the cartilage. Is there any other technique to remedy this problem. I appreciate your help. Thank you. reuel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] intervertebral disc
Jennifer, What do you wish to accomplish histologically? Do you only wish to see the disc material? Do you care about the cranial and caudal vertebral bodies? Are you wanting to perform IHC? Please tell me a little more so that I can provide you with a more detailed options. I am assuming that this is an IVD project and maybe you only wish to see the treatment of the nucleus pulposus? Jack From: jander...@halozyme.com To: histonet@lists.utsouthwestern.edu Date: Wed, 16 Jun 2010 13:30:51 -0700 Subject: [Histonet] intervertebral disc Greetings. What is the best way to process intervertebral disc tissue from pig? Right now I have 4 intact vertebrae with attached discs in formalin. Should I just use a sharp knife? Thanks so much for your expertise. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) jander...@halozyme.commailto:jander...@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_1___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] release of tissues
Hi all, How would you handle a request from a surgeon, f= or release of gallstones or any tissue, to a patient? Assuming a 'Rele= ase of Tissue' request form, indicating the biohazard staus of the tissue,is signed by the patient, is this commom practice? Thanks for your responses, Mary Sue CONFID= ENTIALITY NOTICE: This e-mail and attachments, if any, may contain= confidential information which is privileged and protected from disclosure= by Federal and State confidentiality laws, rules or regulations. This= e-mail and attachments, if any, are intended for the designated ad= dressee only. If you are not the designated addressee, you are hereby = notified that any disclosure, copying, or distribution of this e-ma= il and its attachments, if any, may be unlawful and may subject you= to legal consequences. If you have received this e-mail and its attachmen= ts in error, please contact the St. Mary's Hospital Helpdesk at (301) 4= 75-6166 and delete the e-mail and its attachments from your computer. Than= k you for your attention. Please consider the environment: = do you really need to print this email? CONFIDENTIALITY NOTICE: This = e-mail and attachments, if any, may contain confidential information which = is privileged and protected from disclosure by Federal and State confidenti= ality laws, rules or regulations. This e-mail and attachments, if any, are= intended for the designated addressee only. If you are not the designated addressee, you are hereby notified that any disclosure, copying, or distri= bution of this e-mail and its attachments, if any, may be unlawful and may = subject you to legal consequences. If you have received this e-mail and it= s attachments in error, please contact the St. Mary's Hospital Helpdesk at = (301) 475-6166 and delete the e-mail and its attachments from your computer= . Thank you for your attention. Please consider the environment: do you really need to print this email? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Immuno
Hi All, Does anyone out there know a lab that is able to do a Human Herpes Virus, Type 6 immuno? Thanks for any help on this. We haven't been able to find a reference lab that does this. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fie...@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] release of tissues
Mary Sue, Our hospital regular releases gallstones to patients and/or their doctors. We make a record of it in our computer system as to where and to who the gallstones were sent/released to. Karen Sly BS Biology Pathology Department Central Michigan Community Hospital Mt Pleasant MI. Please consider the environment before printing this e-mail Please consider the environment before printing this e-mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _ From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] To: Ellen Yee [mailto:e...@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
