Re: [Histonet] Disposal of MMA BPO Waste

2010-06-21 Thread Pamela Marcum


I was at UPENN Vet School at the time I used this method.  We sometimes had 4 
to 6 bottles under the hood to polymerize.  We would use Nalgene 1 liter 
bottles about 2/3s full and allow them to polymerize under the hood.  We always 
sat the bottles in large Tri-Pour disposable beakers in case the bottle crack 
or overflowed during polymerization and then through them out in the normal 
waste.  If we had straight MMA we added some BPO to allow it to polymerize 
completely.  Larger amounts would be far more difficult to control the reaction 
in and contain it.  



Pam Marcum 

UAMS 

- Original Message - 
From: Brad Herschler  bhersch2@ illinois . edu  
To: histonet @lists. utsouthwestern . edu 
Sent: Sunday, June 20, 2010 8:50:15 PM 
Subject: [ Histonet ] Disposal of MMA  BPO Waste 

We use an MMA embedding technique similar to that used for histology in our 
lab.  We sent a request to our campus Chemical Waste Management for the MMA 
waste mixture to be disposed of, but they are refusing because they say the 
mixture is too hazardous.  The mixture contains 1gram of benzoyl peroxide 
( BPO ) per 100mL of MMA .  Chemical Waste Management informed us that MMA in 
the presence of BPO is highly reactive. 

We would like to know: for histology, how is MMA + BPO waste normally 
disposed of? 

Thanks 
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RE: [Histonet] Amyloidosis control tissue

2010-06-21 Thread Hannen, Valerie
Missy,
It is my understanding that at this time there are no control tissues 
available. I myself have tried to get some from various places and have been 
told it is very difficult for the vendors to come by them.
 
Valerie Hannen



From: histonet-boun...@lists.utsouthwestern.edu on behalf of MICHELLE BAKER
Sent: Mon 6/21/2010 9:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloidosis control tissue




Hi -

Does anyone know where to buy amyloidosis control tissue?

Thanks-

Missy Baker

 
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RE: [Histonet] Disposal of MMA BPO Waste

2010-06-21 Thread Smith, Allen
This is what comes of letting people with little or no training in chemistry 
call themselves chemical waste mangers.
Yes, methyl methacrylate is highly reactive when mixed with 1% benzoyl 
peroxide: it forms a hard, brittle polymer.  Pour your left over methyl 
methacrylate and benzoyl peroxide into a disposable weighing boat and heat it 
overnight at 60 C and over another night at 80 C.  The resultant polymer is 
commercially sold as Plexiglass; it can be safely disposed of as ordinary 
trash.
A much higher concentration of benzoyl peroxide (10% or more) could explode 
when heated.  Chemical potential depends on concentration.
-Allen A. Smith, M.A. (chemistry), Ph.D. (anatomy)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brad Herschler
Sent: Sunday, June 20, 2010 9:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Disposal of MMA  BPO Waste

We use an MMA embedding technique similar to that used for histology in our
lab.  We sent a request to our campus Chemical Waste Management for the MMA
waste mixture to be disposed of, but they are refusing because they say the
mixture is too hazardous.  The mixture contains 1gram of benzoyl peroxide
(BPO) per 100mL of MMA.  Chemical Waste Management informed us that MMA in
the presence of BPO is highly reactive.

We would like to know: for histology, how is MMA + BPO waste normally
disposed of?

Thanks
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Re: [Histonet] Block disposal

2010-06-21 Thread Angela Bitting
They are classified as Pathological Waste. You should see what your state's 
rules are regarding Pathological Waste.

 histot...@imagesbyhopper.com 6/21/2010 12:33 PM 
Would blocks be considered biohazard waste for purposes of disposal?  I have
blocks stored in an off-site facility and am preparing to dispose of the
blocks.  Can I simply allow the storage place dispose of the blocks in
normal trash, or would I need to have them disposed of as biohazard trash?

On a separate but sort of related issue, does anyone use the Adlex crystals
to dispose of their formalin?  If yes, how do you dispose of the jellied
formalin, in normal trash or in biohazard trash?  I know the mfr says you
can put it in the normal trash, but does anyone trust this??  ;o)

By the way, I am in Florida, if that makes any difference in the answers!

THANKS!

Michelle


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[Histonet] HT or HTL certified?

2010-06-21 Thread Barone, Carol
Nemours is in-search of an HT or HTL certified, or eligible - tech for
Histotechnology Core Facility, Wilmington Delaware. Position 50%
clinical/50% research. Background in enzyme histochemistry, IF and IHC
required. Molecular histology, LMD or Imaging a plus. contact:
cbar...@nemours.org
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RE: [Histonet] Help! In need of positive Gram Control

2010-06-21 Thread Shirley A. Powell
Go to your local drive in mart and buy you a Slim Jim, cut it in thin slices 
and you will have all the Gram controls you can use.  You will never eat 
another Slim Jim.  If you don't know what they are, they are sort of like a 
summer sausage, only thinner and guys love them.  They are found usually right 
beside the check out register.  

Shirley


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dianar...@aol.com 
[dianar...@aol.com]
Sent: Monday, June 21, 2010 7:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help! In need of positive Gram Control

Help! We are in need of positive Gram Control Blocks if anyone has any
extra they are willing to part with.  I have lots of Fungus, Pneumocystis  and
HPV tissue blocks to trade.

Diana Ripley
John Muir Histology
Concord Campus
2540 East Street
Concord, CA 94520
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[Histonet] Histotech job opportunity in Scottsdale, AZ

2010-06-21 Thread Carlos Rodriguez, MD
We are a private practice dermatology group in Scottsdale with an in-house
dermpath lab, and we will be in need of a part-time histotech in the coming
months.

If interested, please reply to this message and attach a CV and/or some
basic info about your credentials and work experience.

Thank you.

Carlos Rodriguez, MD
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