[Histonet] tumour, lymph node and prostate preservation for histology
Hi, I am new to histology and have some very basic questions. I hope somebody out there willing to help me.. I want to isolate tumours from mice and preserve it for histology . I heard that there is different method on how best preserving different organs/ tissue. Could any of you please advise me a method on how to best preserve tumour ? Also if there a method to preserve lymph node and prostate? Thank you very much. Best regards, Carolina Carolina Soekmadji Postdoctoral Research Fellow Australian Prostate Cancer Research Centre - Queensland | Institute of Health and Biomedical Innovation | t: 07 3176 7428 (APCRC - Princess Alexandra Hospital) t: 07 3138 6286 (IHBI - QUT Kelvin Grove) mobile: +61 423 111 807 f: 07 3176 7440 e: carolina.soekma...@qut.edu.aumailto:carolina.soekma...@qut.edu.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] tumour,lymph node and prostate preservation for histology
Carolina, It will depend on what you want to do to the tissue after removal. For Histology, placing the tissue in 10% phosphate buffered formalin is good for morphology, special staains, immunohistochemistry and in situ hybridisation. I have been able to extract RNA and DNA from formalin fixed, paraffin embedded tissues that have been stored at room temperature for 40 years! (and others have also shown this). Contact me for further details. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carolina Soekmadji Sent: Tuesday, 22 June 2010 4:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tumour,lymph node and prostate preservation for histology Hi, I am new to histology and have some very basic questions. I hope somebody out there willing to help me.. I want to isolate tumours from mice and preserve it for histology . I heard that there is different method on how best preserving different organs/ tissue. Could any of you please advise me a method on how to best preserve tumour ? Also if there a method to preserve lymph node and prostate? Thank you very much. Best regards, Carolina Carolina Soekmadji Postdoctoral Research Fellow Australian Prostate Cancer Research Centre - Queensland | Institute of Health and Biomedical Innovation | t: 07 3176 7428 (APCRC - Princess Alexandra Hospital) t: 07 3138 6286 (IHBI - QUT Kelvin Grove) mobile: +61 423 111 807 f: 07 3176 7440 e: carolina.soekma...@qut.edu.aumailto:carolina.soekma...@qut.edu.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Breezy lab/flyaway ribbons!
We have been having trouble with big breeze blowing our precious ribbons out of our hands while cutting. We would like a door on the Histology lab to cut down on the breeze of people walking by through the hall. Our facilities want to find out what other people are doing to stop this problem. We also have air ducts blowing down from above, which is not helping the problem. We would like as many labs solutions as possible. Our facilities have come up with all these crazy barriers that we would have to move to walk around when we need to put our racks on the stainer, answer timers, print more slides, use the oven, etc... Any input would be appreciated! Breezy girls, Josie Britton and Chris Braaten Cheshire Medical Center Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechs needed in Long Island
We have several openings for our fast growing lab. We need Registrations Associates as well. The open positions are: 1 Full time tech with EM experience, 1 full time tech with IHC experience, 2 full time day shift positions and 1 full time overnight position. Excellent opportunity for NYS licensed techs. Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Breezy lab/flyaway ribbons!
Hi Josie and Chris, I've worked in labs with similar problems. Breezes are nice outdoors but cause havoc with ribbons. If you can't get them to put in a door (which you'll probably have to lock since folks will walk in, shut the door and cause a breeze that way) then how about those fuzzy cubicle walls? The ones that are used in prairie dog farms (I mean open office situations ;-) ). Also, you'll need to install some type of baffle or other item that hangs from the air ducts and deflects the air across the ceiling instead of straight down. Good luck! Paula :-) On Tue, Jun 22, 2010 at 6:22 AM, Josie Britton jcbrit...@cheshire-med.comwrote: We have been having trouble with big breeze blowing our precious ribbons out of our hands while cutting. We would like a door on the Histology lab to cut down on the breeze of people walking by through the hall. Our facilities want to find out what other people are doing to stop this problem. We also have air ducts blowing down from above, which is not helping the problem. We would like as many labs solutions as possible. Our facilities have come up with all these crazy barriers that we would have to move to walk around when we need to put our racks on the stainer, answer timers, print more slides, use the oven, etc... Any input would be appreciated! Breezy girls, Josie Britton and Chris Braaten Cheshire Medical Center Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Breezy lab/flyaway ribbons!
For the ceiling vents, you could ask to have deflectors installed. The ones we have are made of stainless, are slightly larger than the vent, and are suspended about 6-8 below the opening. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breezy lab/flyaway ribbons! We have been having trouble with big breeze blowing our precious ribbons out of our hands while cutting. We would like a door on the Histology lab to cut down on the breeze of people walking by through the hall. Our facilities want to find out what other people are doing to stop this problem. We also have air ducts blowing down from above, which is not helping the problem. We would like as many labs solutions as possible. Our facilities have come up with all these crazy barriers that we would have to move to walk around when we need to put our racks on the stainer, answer timers, print more slides, use the oven, etc... Any input would be appreciated! Breezy girls, Josie Britton and Chris Braaten Cheshire Medical Center Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hpylori/Giemsa
Histoland, For those of you who order your H-pylori/Giemsa controls: Who do you use? There were 2 companies that we were using for years, and all of a sudden, there's no bugs in the tissue-many, many boxes were without bugs! And they told my boss that the IHC controls are not 'guaranteed' to show positive for H-pylori and/or Giemsa. We are trying to cut our own, but finding that we need to use more as controls than we actually have-so we're running out frequently. Any help would be greatly appreciated! Thanks so much! HT Amy in Camp Hill, PA www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help! In need of positive Gram Control
You have got to be kidding!! That's hysterical. So process a slim jim and you have Gram - and + controls. If you're serious I'm trying it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:10 AM To: dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Have you tried a Slim Jim? They have gram positive and negative rods in them. Regardless, I still enjoy eating them once and a while! Josie Britton Ht Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dianar...@aol.com Sent: Monday, June 21, 2010 7:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! In need of positive Gram Control
What a waste of a good Slim Jim. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 6/22/2010 7:16 AM, Gill, Caula A. wrote: You have got to be kidding!! That's hysterical. So process a slim jim and you have Gram - and + controls. If you're serious I'm trying it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:10 AM To: dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Have you tried a Slim Jim? They have gram positive and negative rods in them. Regardless, I still enjoy eating them once and a while! Josie Britton Ht Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dianar...@aol.com Sent: Monday, June 21, 2010 7:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! In need of positive Gram Control
A good ol' hot appendix works great. Not as good as a Slim Jim, tho. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Breezy lab/flyaway ribbons!
When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez From: histot...@imagesbyhopper.com To: trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com; histonet@lists.utsouthwestern.edu Date: Tue, 22 Jun 2010 11:10:29 -0400 Subject: RE: [Histonet] Breezy lab/flyaway ribbons! CC: We have a similar situation, but I have shown my techs how to 1) wet the brush they use to take the ribbon off the knife and 2) to roll/curl the ribbon over the damp end of the brush and 3) hover our hand over the top of the ribbon while moving it (to reduce the breeze) from the microtome to the waterbath. I learned this technique when I first started in Histology, as we had a choice, a HOT room with no AC and no wind, or learn how to deal with the breeze and don't sweat! I opted for dealing with the wind. Now, it's second nature and it matters not whether there is wind or not, I still use the same motion for tranfer of the ribbon! Good luck with your situation. :o) Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, June 22, 2010 8:50 AM To: Josie Britton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! For the ceiling vents, you could ask to have deflectors installed. The ones we have are made of stainless, are slightly larger than the vent, and are suspended about 6-8 below the opening. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breezy lab/flyaway ribbons! We have been having trouble with big breeze blowing our precious ribbons out of our hands while cutting. We would like a door on the Histology lab to cut down on the breeze of people walking by through the hall. Our facilities want to find out what other people are doing to stop this problem. We also have air ducts blowing down from above, which is not helping the problem. We would like as many labs solutions as possible. Our facilities have come up with all these crazy barriers that we would have to move to walk around when we need to put our racks on the stainer, answer timers, print more slides, use the oven, etc... Any input would be appreciated! Breezy girls, Josie Britton and Chris Braaten Cheshire Medical Center Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: 06/22/10 06:36:00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slim jims
Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Breezy lab/flyaway ribbons!
Ditto, I use a small brush that I got from the craft store and prior to picking up the ribbon I wet the brush and slip it under my ribbon end closest to the blade and presto no flyaways. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of R J VAZQUEZ Sent: Tuesday, June 22, 2010 2:08 PM To: histot...@imagesbyhopper.com; trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez From: histot...@imagesbyhopper.com To: trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com; histonet@lists.utsouthwestern.edu Date: Tue, 22 Jun 2010 11:10:29 -0400 Subject: RE: [Histonet] Breezy lab/flyaway ribbons! CC: We have a similar situation, but I have shown my techs how to 1) wet the brush they use to take the ribbon off the knife and 2) to roll/curl the ribbon over the damp end of the brush and 3) hover our hand over the top of the ribbon while moving it (to reduce the breeze) from the microtome to the waterbath. I learned this technique when I first started in Histology, as we had a choice, a HOT room with no AC and no wind, or learn how to deal with the breeze and don't sweat! I opted for dealing with the wind. Now, it's second nature and it matters not whether there is wind or not, I still use the same motion for tranfer of the ribbon! Good luck with your situation. :o) Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, June 22, 2010 8:50 AM To: Josie Britton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! For the ceiling vents, you could ask to have deflectors installed. The ones we have are made of stainless, are slightly larger than the vent, and are suspended about 6-8 below the opening. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breezy lab/flyaway ribbons! We have been having trouble with big breeze blowing our precious ribbons out of our hands while cutting. We would like a door on the Histology lab to cut down on the breeze of people walking by through the hall. Our facilities want to find out what other people are doing to stop this problem. We also have air ducts blowing down from above, which is not helping the problem. We would like as many labs solutions as possible. Our facilities have come up with all these crazy barriers that we would have to move to walk around when we need to put our racks on the stainer, answer timers, print more slides, use the oven, etc... Any input would be appreciated! Breezy girls, Josie Britton and Chris Braaten Cheshire Medical Center Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: 06/22/10 06:36:00
[Histonet] Re: Autofluorescence in murine white adipose tissue cryosections
Dear Frank, Good luck with ridding your samples of autofluorescence. Fat is always very brightly fluorescent and I suspect it might even be so without any aldehyde fixation. First I wondered how successful you'd be using Sudan Black B, knowing it is a fat stain, and knowing that it's been published for this purpose. So you might try just that and see how that works. In addition you could try using a combination of UV irradiation and the Sudan Black B and see if the combination works for you. They use paraffin sections in this reference, but it might work as well with cryosections. http://www.microscopyu.com/references/pdfs/Viegas_etal_Eur_J_Histochem-51-59-2007.pdf Good luck, and do report back if you find a solution to this dilemma! Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slim jims
I just found a partially eaten Slim Jim snack picture on wikipedia... Is it Friday again? :- Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oven for paraffin slide drying
Do slide warmers cost too much? I don't know how much they cost, though, maybe an oven is cheaper. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Tue, Jun 22, 2010 at 7:06 PM, Mia Woodruff mia.woodr...@qut.edu.au wrote: Hello all, I need to purchase an oven for drying slides, on a very limited budget. I came across a food dehydrator which looks like an oven and has drawers which are the perfect size for the slide holders to fit into and it can reach a temperature of 60C - and it costs about 1/8 of the price of a normal oven. Can anyone foresee any issues in me using this for drying paraffin slides? As far as I can tell there is a low air flow inside and the temp can be set up to 60 degrees so it seems perfect? Any comments? Thanks Mia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help! In need of positive Gram Control
Tried the slim jim and all of my doctors did not like it. Don't waste your time. Connie G. Date: Tue, 22 Jun 2010 10:16:14 -0400 From: cg...@marylandgeneral.org To: jcbrit...@cheshire-med.com; dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control CC: You have got to be kidding!! That's hysterical. So process a slim jim and you have Gram - and + controls. If you're serious I'm trying it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:10 AM To: dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Have you tried a Slim Jim? They have gram positive and negative rods in them. Regardless, I still enjoy eating them once and a while! Josie Britton Ht Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dianar...@aol.com Sent: Monday, June 21, 2010 7:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendarocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
Tom: As much as I agree with your acknowledgment that its seems a bit odd for the CAP to have a blood-banker responding to AP-related issue, I'm actually not surprised. The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time, and they wonder why IHC isn't expected to follow the same requirements as chemistry, immunology, etc. -- IHC is, after all, an awful lot like ELISA. And rightfully so, because IHC is, under CLIA (which supersedes CAP), considered highly-complex, non-waived testing -- and is, therefore, subject to the same Quality Systems regulations (in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. Could it be that, because AP produces qualitative results that are interpreted by a pathologist and CP produces quantitative results that are interpreted by an analyzer, we somehow think that CLIA rules don't apply to IHC? I certainly don't have the answer to that, but it make me wonder what the future holds. As witnessed by some of the newest CAP 'standards' (including the question in question...no pun intended), e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens must be tested, and where 10 of the positives must be weakly positive -- an acknowledgment that validation specimens must be carefully selected in order to obtain appropriate results), it certainly doesn't appear that the regulation of IHC testing is going to become more relaxed. Joe Myers, M.S., CT(ASCP) -- Message: 12 Date: Fri, 18 Jun 2010 12:38:07 -0700 From: Thomas Jasper tjas...@copc.net Subject: RE: [Histonet] New CAP question ANP.22760 To: Mark Tarango marktara...@gmail.com Cc: _histo...@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of contemporaneously staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - Trying to put the yoke of clinical lab onto anatomic path. We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC tissue lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
