[Histonet] Histology techs grossing in specimens???
Trying to get some feed back on histology techs grossing in for the doctors..My main question would be the training involved? Can anyone just start to gross in biopsies and small specimens. What does it include and what sort of education is involved if any Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
We retain our containers and specimens for 3 weeks. Josie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Wednesday, June 23, 2010 5:51 PM To: Hartz, Rhonda SktnHR; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) We retain them until the specimen is signed out, usually no more than 3 days. This has been helpful if the specimen container labeling is called into question either by the pathologist (because the cellular profile does not match what the specimen source indicates) or the clinician. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hartz, Rhonda SktnHR Sent: Tuesday, June 22, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.ha...@saskatoonhealthregion.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ARTICLE
Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called Workplace Violence in the Laboratory Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histology techs grossing in specimens???
We do all of our grossing and use the CLIA regulations: TESTING PERSONNEL (42 CFR 493 1489) 1. Licensed MD, DO or DPM. 2. Doctorate, master's, or bachelor's in laboratory science. 3. Education and training equivalent to an associate degree in a laboratory science or medical laboratory technology that includes at least 60 semester hours including 24 semester hrs of medical lab technology and at least 3 months training in each specialty in which high complexity testing is performed; or, 60 semester hrs including 24 hrs of science that includes 6 hrs chemistry, 6 hrs biology, and 12 hrs chemistry, biology or, medical lab tech in any combination and laboratory training that includes either: completion of a clinical lab training program and at least 3 months training in each specialty in which high complexity testing is performed. It is my understanding that if there is no cutting involved (i.e. counting and measuring GI biopsies) it is not considered a high complexity task and anyone in the lab would be able to gross in that regard. Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 Trying to get some feed back on histology techs grossing in for the doctors..My main question would be the training involved? Can anyone just start to gross in biopsies and small specimens. What does it include and what sort of education is involved if any Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ARTICLE
No - but I could write one. From: Sara Baldwin/mhhcc.org sbald...@mhhcc.org To: histonet@lists.utsouthwestern.edu Date: 06/25/2010 10:53 AM Subject: [Histonet] ARTICLE Sent by: histonet-boun...@lists.utsouthwestern.edu Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called Workplace Violence in the Laboratory Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ARTICLE
http://laboratorian.advanceweb.com/Editorial/Search/Searchresult.aspx?KW=violence+in+the+workplace From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org [sbald...@mhhcc.org] Sent: Friday, June 25, 2010 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ARTICLE Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called Workplace Violence in the Laboratory Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Prepared Solutions
Hello All, I work in a small lab where we purchase most of our chemicals already prepared. The only solutions we routinely prepare ourselves are our acid alcohol and acid water for our staining procedures, Carnoy's Fixative (since it needs to be fresh) and our 50%, 70% etc alcohol solutions for our Pap stain and for our processing machine. I would also like to start preparing our 1% and 3% acetic acid solutions instead of purchasing them since I have glacial acetic acid on hand anyway for our Carnoy's Fixative (not that the acetic acid acid solutions are expensive but every dollar counts, plus the shipping charges always shock me). My questions are 1 - in your labs, do you have a policy with instructions on how to prepare each solution? (eg 1 ml acetic acid diluted with water to 100 ml to prepate 1% Acetic Acid Aq and is it neccessary to say 500 ml water and 500 ml reagent alcohol to form 50% alcohol) 2 - when you label the solutions you prepare, do you transfer the lot numbers and expiration dates of the chemicals that were used to make the solutions onto the new chemical bottles and use the expiration date of the concentrated chemical as the expiration date of the prepared chemical? Thanks in advance for all input. Im always amazed at how fast, how many, and how thorough/helpful the responses are! Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re Grossing assistants
I would suggest that you review the archives on this topic. There has been much discussion recently. The short answer is that ANYONE performing ANY grossing in the AP laboratory must meet the requirements for high complexity testing. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] too blue
Any ideas why patient tissue stained with Trichrome would be too blue when the controls are working perfectly? We've repeated the stain and it keeps happening. Thanks Stacy McLaughlin HT(ASCP) Lead Histology Tech./Laboratory Safety ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grossing Personnel Requirements
Hi All, I hope this will help to clarify the new ruling passed down from CAP regarding personnel requirements for grossing specimens. Historically, CAP differentiated between grossing and processing. Grossing required orientation and/or dissection of the specimen and was considered high complexity testing. In contrast, processing did not require specific orientation and minimal cutting and was not considered high complexity. However, CAP now considers grossing and processing to be in one category and is all high complexity. I will excerpt the CAP alert and other pertinent information below: March 31, 2010 Attention Anatomic Pathology Laboratories: In preparation for the release of the 2010 CAP Checklist Edition in June of this year, CAP is notifying all accredited anatomic pathology laboratories of a revised checklist requirement that may have an impact on your laboratory's staffing. The revisions will require that all non-pathologist individuals who perform macroscopic tissue examinations meet the personnel requirements for high complexity testing in accordance with CLIA. This interpretation of the CLIA requirement was recently provided to CAP from CMS. As a service to CAP Accredited laboratories, the CAP offers compliance alerts to help your laboratory maintain continuous compliance. Previously, the Anatomic Pathology checklist differentiated two levels of macroscopic examination, processing and grossing. In this context, processing means macroscopic examination of small specimens not requiring knowledge of anatomy, which are entirely submitted for microscopic examination, while grossing means macroscopic examination of more complex specimens. Unlike individuals who performed grossing, individuals who performed processing were not required to be qualified as high complexity testing personnel. In the 2010 checklist edition, the concept of macroscopic tissue processing will no longer be recognized. All macroscopic tissue examinations will be considered to be grossing. Therefore, any individual who performs macroscopic tissue examinations must be a pathologist, pathology resident, or an individual qualified to perform high complexity testing under the supervision of a pathologist (refer to ANP.11610, below). Please contact the Laboratory Accreditation Program at (800) 323-4040, option 1, then 4, or 1-847-832-7000, or by email if you have any questions. ANP.11610 Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. The CLIA regulations on high complexity testing personnel may be found at HC Testing Personnel. In addition, the CLIA regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at the above Web address and at Grandfathered Exceptions. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. Sorry it is rather lengthy, but it should put to rest most of your questions regarding the new regulation and how it impacts labs that utilize non-Pathologists for grossing surgical specimens. Best regards, Sheri S Sheri Meilus Anatomic Pathology Supervisor Bay Pines VAHC Building 100 Room 2B-126 727-398-6661 ext 4596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Friday, June 25, 2010 1:22 PM To:
[Histonet] Full-time day Job open in Central Ohio..
We have and opening for a full time day shift histotech. We are a small but progressive hospital of around 250 beds in Newark, Ohio (about 30 miles from Columbus). If interested you may apply through our website (www.lmhealth.orghttp://www.lmhealth.org/) Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Prepared Solutions
Long, long ago, we made everything up (mostly from powders), we kept all the procedures written on recipe cards filed alphabetically in a recipe box and it worked very well. About the only thing we make up anymore is formalin, and graded alcohols. I have a procedure for the formalin but none for the alcohols. We labeled the working solutions with the chemical name, percentage, and a shelf life date. Dry powders lasted forever (many did not even give an expiration) so we just fabricated a reasonable shelf life date. I also think it is advisable to list and spell out the name and concentration of every ingredient used. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Friday, June 25, 2010 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prepared Solutions Hello All, I work in a small lab where we purchase most of our chemicals already prepared. The only solutions we routinely prepare ourselves are our acid alcohol and acid water for our staining procedures, Carnoy's Fixative (since it needs to be fresh) and our 50%, 70% etc alcohol solutions for our Pap stain and for our processing machine. I would also like to start preparing our 1% and 3% acetic acid solutions instead of purchasing them since I have glacial acetic acid on hand anyway for our Carnoy's Fixative (not that the acetic acid acid solutions are expensive but every dollar counts, plus the shipping charges always shock me). My questions are 1 - in your labs, do you have a policy with instructions on how to prepare each solution? (eg 1 ml acetic acid diluted with water to 100 ml to prepate 1% Acetic Acid Aq and is it neccessary to say 500 ml water and 500 ml reagent alcohol to form 50% alcohol) 2 - when you label the solutions you prepare, do you transfer the lot numbers and expiration dates of the chemicals that were used to make the solutions onto the new chemical bottles and use the expiration date of the concentrated chemical as the expiration date of the prepared chemical? Thanks in advance for all input. Im always amazed at how fast, how many, and how thorough/helpful the responses are! Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vet Dx Labs
If you are the tech for one of the States' vet dx labs, could you please contact me; I have a question about the Ventana NexES and the service contract ending. I don't want to send out to the NVSL list because my list is from 2003 and I'm sure it's changed. Once I have all your names, I'll send out one email with one question about the NexES. Thanks! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Prepared Solutions
The preparation of your solutions should be included in your SOP. The lot numbers of your chemicals cannot be transferred to the solutions you prepare because they are a different thing. You should determine the shelf time of your working solutions and write it in the label, along with the date they were prepared. René J. --- On Fri, 6/25/10, Brandi Higgins brandihigg...@gmail.com wrote: From: Brandi Higgins brandihigg...@gmail.com Subject: [Histonet] Prepared Solutions To: histonet@lists.utsouthwestern.edu Date: Friday, June 25, 2010, 12:33 PM Hello All, I work in a small lab where we purchase most of our chemicals already prepared. The only solutions we routinely prepare ourselves are our acid alcohol and acid water for our staining procedures, Carnoy's Fixative (since it needs to be fresh) and our 50%, 70% etc alcohol solutions for our Pap stain and for our processing machine. I would also like to start preparing our 1% and 3% acetic acid solutions instead of purchasing them since I have glacial acetic acid on hand anyway for our Carnoy's Fixative (not that the acetic acid acid solutions are expensive but every dollar counts, plus the shipping charges always shock me). My questions are 1 - in your labs, do you have a policy with instructions on how to prepare each solution? (eg 1 ml acetic acid diluted with water to 100 ml to prepate 1% Acetic Acid Aq and is it neccessary to say 500 ml water and 500 ml reagent alcohol to form 50% alcohol) 2 - when you label the solutions you prepare, do you transfer the lot numbers and expiration dates of the chemicals that were used to make the solutions onto the new chemical bottles and use the expiration date of the concentrated chemical as the expiration date of the prepared chemical? Thanks in advance for all input. Im always amazed at how fast, how many, and how thorough/helpful the responses are! Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] manual morphometric analysis
hi all histonetters i need to do some morphomtric analysis on some samples of small intestin but we have no image analysis software so i need to do it manually by occular micrometer. i need to know how to calculate villus surface area? overall mucosal surface area of cross section? surface area of lamina propria? mitotic figure index?? i don't know how to caculate all . any help please thanks in advance mohamed faculty of vet. med. cairo univ.- egypt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] MMA Formulation for EXAKT Grinding
Nikki, The MMA formulation you describe is one that yields a very soft block suitable for thin section (4-6 microns) microtomy. I have heard where people use this formulation for undemineralized bone and maybe even for stent work, but the softness of this formulation concerns me for the stents given the reduced stability. For me personally it is not what I use for both of these specimen types, but that is for another discussion. It is important to note that the volume of catalyst is proportional to the total volume of solution and thus this ratio (expressed as w/w) is directly proportional to the reaction product. Now to make things a little more confusing, the reaction product is influenced by air temperature, the size/density of the specimen, the total volume of reaction product, and sometimes the embedding container and void space above the solution level and container lid. Furthermore, since this reaction or polymerization of resin is an exothermic reaction, the rate (expressed as a unit of time) at which the reaction reaches the actual point at which polymerization initiates (v-max) also then influences the amount of heat that is generated from the reaction. This then is proportional to the quality of polymerization that can be seen as either a hard clear desirable block or and over polymerized, bubbled mess!!! It is my opinion that the bubbles in your specimen blocks are related to the build up of pressure in your container and caused by a rapid polymerization of your specimens by the use of the heated water bath (as per you concentration of catalyst to MMA/DBP solution) and lack of void space to buffer or diffuse excess heat. My feeling is that you are using too much catalyst in conjunction with the heat of the water bath to polymerize these specimens. Also, what is the volume of the solution you are polymerizing, how close are your specimen molds to each other in the water bath, and is the water level of the water bath at or above the embedding solution level in the specimen container? The heat generated from one specimen can sometimes add to the heat generated by another in close proximity. This then results sometimes in an over polymerization of one specimen (too much heat generated in the reaction) and no polymerization of another (absence of heat to drive the reaction). Here are my suggestions: 1) If you need specimens polymerized immediately the next day, take care to space out your specimens further apart in the water bath. Also, try turning down the water bath to reduce the secondary heat used to drive the reaction. If none of this works, then look at reducing the amount of catalyst used (may want to do this first and keep everything else the same). 2) If you can spare a few days, don't change a thing with the embedding solution, try switching your molds to polypropylene containers and leave them out on the counter at room temperature (22-23C) for 2-3 days until they polymerize. Hope this helps and it wasn't too confusing. Jack On Jun 25, 2010, at 3:46 PM, Wahlberg, Nikki nikki.wahlb...@bsci.com wrote: Hello Everyone, I was wondering if you could help me with my MMA formulation. I have been using a formulation that I found in a published paper. My current embedding formulation is 80ml MMA, 20ml Dibutyl Phthalate and 3g Benzoyl Peroxide. The samples are embedded after three days of infiltration, one change per day, with the formulation of 80ml MMA and 20ml Dibutyl Phthalate. Lately have noticed that there is a pressure build up in the vials. I have had a few vials burst almost immediately once placed in the heated waterbath. I am filling the glass vials full with the embedding solution, capping them and then placing them in a water bath in a 37 degree oven. They are completely polymerized by the next morning. I am also getting bubbles in the plastic when polymerized. I have two questions: Is there any way to get rid of the bubbles in the plastic and of more concern what do you think is causing the pressure build up? I would really appreciate any help that you can provide. Thank you, Nikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Lead Tech Position
Our lab is currently seeking a highly motivated histotech with a *_strong_* background in immunohistochemistry. We are located just outside St. Louis Missouri. The right candidate will take the lead role in the development and implementation of new procedures, instrumentation and quality control for the IHC department. Must have the ability to recognize probable cause of technical difficulties and to expediently remedy these difficulties. This is a full-time position, Monday-Friday. Please contact me if interested. Lisa Hamilton, HT (ASCP) Histology Supervisor (314) 991-4363 x 230 lhamil...@wcplaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] manual morphometric analysis
If you have a microscope camera of some kind, ImageJ is a free dowmloadable software package that will do morphometric analysis. See: http://rsbweb.nih.gov/ij/. There is a mailing list with a lot of helpful people on it too. Bob Nienhuis UCLA / VA Medical Center Los Angeles On Fri, Jun 25, 2010 at 2:59 PM, mohamed abd el razik k8...@yahoo.comwrote: hi all histonetters i need to do some morphomtric analysis on some samples of small intestin but we have no image analysis software so i need to do it manually by occular micrometer. i need to know how to calculate villus surface area? overall mucosal surface area of cross section? surface area of lamina propria? mitotic figure index?? i don't know how to caculate all . any help please thanks in advance mohamed faculty of vet. med. cairo univ.- egypt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ARTICLE
I have it some where at work. I seem to remember that it was a work shop and not just an article I read. I will look for it. Pearl Dance like no one is watching In a message dated 6/25/2010 11:19:19 A.M. Central Daylight Time, Jackie.O'con...@abbott.com writes: No - but I could write one. From: Sara Baldwin/mhhcc.org sbald...@mhhcc.org To: histonet@lists.utsouthwestern.edu Date: 06/25/2010 10:53 AM Subject: [Histonet] ARTICLE Sent by: histonet-boun...@lists.utsouthwestern.edu Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called Workplace Violence in the Laboratory Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processors
We are in the process of trying out tissue processors. Are there any users of the Leica Peloris or Thermo EG who can help us out with some opinions? Reliability, ease of changing solutions, programmability? Thanks for any help. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
