[Histonet] Position open in Maryville TN

2010-07-01 Thread rgrow

Blount Memorial Hospital in Maryville, TN has a full-time histology
technician position open weekdays.
If you are interested in working for a hospital that cares about its
patients, we may be what you are looking for. We have a new (3yrs old)
histology laboratory, with excellent ventilation, and ample workspace. We
do approximately 12,000 cases/yr routine small and large surgical’s, 
biopsies with frozens, routine HE, special stains, and (automated) IHC
antibodies. 3 tissue processors, 2 run nightly, with one for same-day
turn-around cases.
We are located just minutes from the beautiful Smoky Mountains National
Park, Gatlinburg and Pigeon Forge. With 4 wonderful seasons, all types of
outdoor activities are possible.  Maryville is host to the annual Foothills
Fall Festival with top name entertainment and crafts, and just 30 minutes
from Knoxville's culture events and entertainment, as well as UT football,
basketball, etc.
General histology experience with certification preferred. Some
Immunohistochemistry would be helpful.
Good benefits are offered. Please visit our website at blountmemorial.org.
to fill out an application and attach a resume.

Renee Grow, BA., HT (ASCP)
rg...@bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax


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Re: [Histonet] Chilling Tray for Blocks

2010-07-01 Thread Kim Merriam
we use something like this:
https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=414004-256inE=1highlight=414004-256

we keep several sizes in the freezer, depending on how many blocks we need to 
cut.  The nice thing is that it is cheap and you can hydrate your blocks at the 
same time you are chilling them.

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 





From: Sherwood, Margaret msherw...@partners.org
To: Jay Lundgren jaylundg...@gmail.com; Laurie Colbert 
laurie.colb...@huntingtonhospital.com
Cc: histonet@lists.utsouthwestern.edu
Sent: Wed, June 30, 2010 4:35:53 PM
Subject: RE: [Histonet] Chilling Tray for Blocks

We actually use a styrofoam box (used for shipping) and it stays cold longer
with very little melting.  We keep a couple in the -20 freezer. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
Sent: Wednesday, June 30, 2010 4:01 PM
To: Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Chilling Tray for Blocks


    Fill a Tupperware container with water, pop it in the freezer, and
tomorrow you will have a portable block chiller, with the blocks at a
perfect level for your needs, for 1/100th the price.

                                                        Not trying to be a
smarta$$, but


Jay A. Lundgren, M.S., HTL (ASCP)
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RE: [Histonet] Chilling Tray for Blocks

2010-07-01 Thread WILLIAM DESALVO

We use HistoCool trays, two sizes available, two trays for each microtome. One 
is always in the freezer. They work great at the microtomy bench, stay cold for 
long periods of time, have a styrofoam tray they sit in and have a lid.

William DeSalvo, B.S., HTL(ASCP)




 
 Date: Thu, 1 Jul 2010 05:38:25 -0700
 From: kmerriam2...@yahoo.com
 To: msherw...@partners.org; jaylundg...@gmail.com; 
 laurie.colb...@huntingtonhospital.com
 Subject: Re: [Histonet] Chilling Tray for Blocks
 CC: histonet@lists.utsouthwestern.edu
 
 we use something like this:
 https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=414004-256inE=1highlight=414004-256
 
 we keep several sizes in the freezer, depending on how many blocks we need to 
 cut.  The nice thing is that it is cheap and you can hydrate your blocks at 
 the same time you are chilling them.
 
 Kim
  Kim Merriam, MA, HT(ASCP)QIHC
 Cambridge, MA 
 
 
 
 
 
 From: Sherwood, Margaret msherw...@partners.org
 To: Jay Lundgren jaylundg...@gmail.com; Laurie Colbert 
 laurie.colb...@huntingtonhospital.com
 Cc: histonet@lists.utsouthwestern.edu
 Sent: Wed, June 30, 2010 4:35:53 PM
 Subject: RE: [Histonet] Chilling Tray for Blocks
 
 We actually use a styrofoam box (used for shipping) and it stays cold longer
 with very little melting.  We keep a couple in the -20 freezer. 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
 Sent: Wednesday, June 30, 2010 4:01 PM
 To: Laurie Colbert
 Cc: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Chilling Tray for Blocks
 
 
 Fill a Tupperware container with water, pop it in the freezer, and
 tomorrow you will have a portable block chiller, with the blocks at a
 perfect level for your needs, for 1/100th the price.
 
 Not trying to be a
 smarta$$, but
 
 
 Jay A. Lundgren, M.S., HTL (ASCP)
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 contains patient information, please contact the Partners Compliance HelpLine 
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 http://www.partners.org/complianceline . If the e-mail was sent to you in 
 error
 but does not contain patient information, please contact the sender and 
 properly
 dispose of the e-mail.
 
 
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[Histonet] Immuno Tech position Dallas Sun-Th

2010-07-01 Thread Pat Patterson
IMMUNOHISTOCHEMISTRY TECHNICIAN

 

Sun - Thurs 12am - 8:30am

 

ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas 

is seeking an Immunohistochemistry Technician.  Responsibilities include
slide preparation (paraffin and frozen sections),

manual IHC staining, antibody titer preparation, equipment maintenance,
supply and reagent inventory maintenance, 

and QC/QA record maintenance.

 

We require a minimum of 1 year full-time experience as a histotech.  

 

ProPath utilizes leading technology and is a quality oriented pathology
laboratory.  

Our benefits include medical, dental, company paid short-term and long-term
disability insurance, a matched 401K plan and more!

 

For consideration send resume to: 

 

ProPath, Human Resources

1355 River Bend Drive

Dallas, TX 75247

Fax - 214/237-1825

Job Line - 214/237-1775

j...@propath.com

 

EOE

 

Angie Viancourt

Senior Human Resources Generalist

ProPath(r) - The Leader in Pathology Services

1355 River Bend Drive

Dallas, TX 75247

214-237-1613

214-237-1813 Fax

 

To Learn more about ProPath, please visit http://www.ProPath.com
http://www.ProPath.com  mailto:angie.vianco...@propath.com 

 

Don't Follow the Leader. Join the Leader!

 

 

 

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[Histonet] Chilling trays

2010-07-01 Thread Breeden, Sara
I went to eBay and found a set of three (total $12.00) stainless steel
food prep trays, roughly 9x5x2 (LxWxH). I fill them with DI water and
freeze them.   I also inherited a larger stainless steel container
that's 12.5x10.5x4.5 - same deal, DI water and freeze.   I face blocks,
put them on the ice and add a little DI water and soak for 10-15 minutes
- blocks cut like a dream! They stay frozen for a  l - o - n - g  time!
Try a local restaurant supply.  

 

Sally Breeden, HT(ASCP)

Veterinary Diagnostic Services

New Mexico Department of Agriculture

700 Camino de Salud NE

Albuquerque, NM  87108

505-841-2576

 

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[Histonet] RE: Chilling trays

2010-07-01 Thread Helen Fedor
You can try disecting trays. I just googled discting tray, looks like listing 
price is 17.00. they are the perfect size and shape for freezing. and keep 
blocks organized.


Helen Fedor
Johns Hopkins University
TMA and Prostate Spore Lab Manager
JHU SOM Uro/Pathology
600 N. Wolfe Street, Marburg Room 406
Baltimore MD, 21287-7065
410-614-1660 / Fax 410-614-3695
Pager 410-283-3419



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara 
[sbree...@nmda.nmsu.edu]
Sent: Thursday, July 01, 2010 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Chilling trays

I went to eBay and found a set of three (total $12.00) stainless steel
food prep trays, roughly 9x5x2 (LxWxH). I fill them with DI water and
freeze them.   I also inherited a larger stainless steel container
that's 12.5x10.5x4.5 - same deal, DI water and freeze.   I face blocks,
put them on the ice and add a little DI water and soak for 10-15 minutes
- blocks cut like a dream! They stay frozen for a  l - o - n - g  time!
Try a local restaurant supply.



Sally Breeden, HT(ASCP)

Veterinary Diagnostic Services

New Mexico Department of Agriculture

700 Camino de Salud NE

Albuquerque, NM  87108

505-841-2576



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RE: [Histonet] Chilling trays

2010-07-01 Thread Sherwood, Margaret
We used the metal trays as well for years.  Switched to the styrofoam boxes and
they stay frozen longer.  Just a thought. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Thursday, July 01, 2010 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Chilling trays

I went to eBay and found a set of three (total $12.00) stainless steel
food prep trays, roughly 9x5x2 (LxWxH). I fill them with DI water and
freeze them.   I also inherited a larger stainless steel container
that's 12.5x10.5x4.5 - same deal, DI water and freeze.   I face blocks,
put them on the ice and add a little DI water and soak for 10-15 minutes
- blocks cut like a dream! They stay frozen for a  l - o - n - g  time!
Try a local restaurant supply.  

 

Sally Breeden, HT(ASCP)

Veterinary Diagnostic Services

New Mexico Department of Agriculture

700 Camino de Salud NE

Albuquerque, NM  87108

505-841-2576

 

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[Histonet] MIB-1 staining

2010-07-01 Thread laurie

Hello everyone, 

Happy early 4th of July.  I was wondering if anyone out there is doing MIB-1 
staining in mouse with human xenograft tissue?  We are currently using Dako's 
MIB-1 clone which works well in the human ( which is what our target is) but it 
also has some background issues depending on the mouse line used to host the 
xenograft.

Any thoughts?  I have looked at rabbit monoclonals but I am primarily finding 
SP6 as the clone there.

Thanks, 
Laurie 

Laurie Popp, HT ( ASCP)cm

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RE: [Histonet] HE slide cost

2010-07-01 Thread WILLIAM DESALVO

Thank you for the direction to CAP, but I am registered on the CAP site and I 
have not been able to find a cost per slide calculation for HE. I would 
welcome the assistance of CAP to help standardize the process and provide 
specific direction and guidance.
 
On the CAP site there is a paper by Dr. Hendricks, Cleveland Clinic. He does 
discuss cost per slide associated w/ digital pathology and capturing the FTE 
time required to scan, but even that discussion does not consider the total 
cost for a slide. The difficulty is that there is no standardization of process 
or procedure and the digital age means an increase in labor costs for the 
technical end of the process. We are rapidly moving to digital pathology and 
our lack of standardization will create many problems similar to those that are 
experienced when labs perform IHC. How do we progress Histotechnology from Open 
Standards to Interoperability, a must if we truly want to compare results?
 
What continues to concern me most, many Histology labs not only do not have an 
accurate cost per slide for HE, they do not have a cost for special staining 
(Histochemical techniques) or IHC and they cannot break down each step of their 
process to consider the cost. Too many Histology labs rely on their vendors to 
provide costing information. In the past, there have always been enough dollars 
available in the reimbursements to more than cover the technical component, 
but as we move forward and to more precise and complex procedures, while 
requiring better trained and educated technologists, the dollars will quickly 
become inadequate. We cannot continue to function as we do today.
 
In the future state of Histology and Histotechnology, it will be essential that 
each Histology lab be able to efficiently manage the technical process, remove 
waste and maintain a healthy profit margin, while providing excellent quality 
and patient care. There must be an increase in automation of instruments and 
even though we do not perform our tests in a test tube, more tests performed in 
Histology will be at the IVD level. We must become better at Histotechnology to 
continue to perform more complex tests and we will also need a better 
partnering of the Histotechnologist and Pathologist to keep up with the rapid 
advancement of technology.
 
Unfortunately, we will have many Histology labs struggle with this change and 
many will go out of business or be consolidated into larger labs that can 
afford the technology, through efficient process improvement, and control of 
quality and costs. The bright side is this is an exciting time for Histology 
and Histotechnology and I am certainly excited about the future.  

William DeSalvo, B.S., HTL(ASCP)




 




Subject: RE: [Histonet] HE slide cost
Date: Wed, 30 Jun 2010 14:26:31 -0700
From: jhowe...@yrmc.org
To: wdesalvo@hotmail.com




Register on the CAP site. They have many documents on calculation of different 
costs in the laboratory.



From: histonet-boun...@lists.utsouthwestern.edu on behalf of WILLIAM DESALVO
Sent: Wed 6/30/2010 11:09 AM
To: cmil...@physlab.com; rcar...@harthosp.org; histonet
Subject: RE: [Histonet] HE slide cost



I agree w/ Cheryl, there are many variables to consider and there is no 
standard cost for an HE because we, Histotechnology, do not have a 
standardized process for HE. The calculation requires precise data and will 
often be higher than you anticipate. I find that most labs, and the vendors, do 
not account for the total cost. My cost calculation is as follows:



(Materials/Reagents + Instrumentation + Labor + Company Allocation) / Unit 
(i.e. Case/Specimen/Cassette/Slide/Stain) = Cost



You will need assistance from your Finance department to acquire many of the 
costs associated with your instruments, labor and especially the allocation 
back to your department for shared services.



I also feel that Cheryl's cost per slide is definitely in the ballpark. I have 
worked at labs, small to very large, and the cost per slide ranges in the $8.00 
- $10.00 / slide, when all department expenses are accounted and the proper 
allocation of technical time is applied.

William DeSalvo, B.S., HTL(ASCP)





 From: cmil...@physlab.com
 To: rcar...@harthosp.org; histonet@lists.utsouthwestern.edu
 Date: Wed, 30 Jun 2010 11:51:13 -0500
 Subject: RE: [Histonet] HE slide cost
 CC:

 There are so many variables, it all depends on your operating costs i.e. 
 Labor, reagents, maintenance contracts and capitol equipment if applicable. I 
 just completed my cost analysis today and it costs my department 
 approximately $8.15 to produce 1 HE slide, $2.55 each Special and $38.71 for 
 each IHC Antibody.

 Cheryl A. Miller HT(ASAP)cm
 Histology/Cytology Prep Supervisor
 Physicians Laboratory Services
 Omaha, NE. 402 731 4145 ext. 554
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
 Sent: 

[Histonet] bat wing histology

2010-07-01 Thread Mohit Chadha
Hello everyone,

This is my very first post and I am desperately looking for help. I am new
to histology, so any help would be much appreciated.

I am studying the peripheral sensory innervation of bat wings. As a first
step, I would like to demonstrate the innervation pattern on the different
parts of the wing membrane (a whole mount of the wing?). Second, I would
like to demonstrate the mechanoreceptor make-up of the tiny hairs on the
wing membrane.

Bat wings are highly elastic, with numerous folds, a thickness of about
35-45 microns (in the species I study), a network of thin collagen bundles,
and pigmented superficial epidermal layers.
I could provide more information if required.

Hoping to hear back from the members.
Thank you.
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[Histonet] Re: replacement Turtox Internal Ear, cochlea slides

2010-07-01 Thread Atoska Gentry
Hello! please see below inquiry from a colleague. Thanks! Atoska


Our histology class is looking for replacements for old Turtox (Guinea
Pig) Internal Ear, cochlea slides and was wondering if anyone knew a
company who supplied these slides or tissue blocks? I don't think it has
to be guinea pig but I haven't been able to locate internal ear slides
from any species.  Any help would be appreciated.  Thanks!

~~~
Michelle (Shelly) Aono
Histology Technician IV
CVM  AU Staff 
Advisory Council Representative
Auburn University
CVM-APP
212 Greene Hall, Auburn, AL 36832
(334) 844-5594


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[Histonet] Dallas job

2010-07-01 Thread LINDA MARGRAF

 
Here's a job someone asked me to post on Histonet (Please don't reply to me 
Thanks LM) 


OPEN POSITION FOR AN EXPERIENCED HISTOTECHNOLOGIST IN THE GREATER DALLAS, TX 
AREA:

 Customer Liaison/Technical Manager

70K+Bonus


My name is Lisa Chubinsky and I am an Executive Recruiter with Scientific 
Professional Search. My client, which is a Medical Products company that 
manufactures Histotechnology products,  currently seeks a Customer 
Liaison/Technical Manager to oversee Technical Support, Customer Outreach, 
Sales Support and Marketing and Product Development. The Technical Manager will 
be expected to advance the image and reputation of the Company through frequent 
speaking engagements at national and regional trade shows. The Technical 
Manager will be given ownership of the Technical Manual in order to make sure 
its contents are accurate, up to date, and being used effectively as a 
marketing and education piece. Other customer outreach projects might also 
include a periodic newsletter and development and nurturing of a Histotech 
Advisory Board to be used by the Company to stay current with issues and 
challenges faced by Histotechs.

 

For immediate consideration, please contact Lisa Chubinsky, CPC, Scientific 
Professional Search, 203-834-2100x12, l...@spexecsearch.com 

 






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Re: [Histonet] bat wing histology

2010-07-01 Thread V. Neubert
 Make that an open discussion on the list, no under cover posting if
possible, please! :)
 Hello everyone,

 This is my very first post and I am desperately looking for help. I am new
 to histology, so any help would be much appreciated.

 I am studying the peripheral sensory innervation of bat wings. As a first
 step, I would like to demonstrate the innervation pattern on the different
 parts of the wing membrane (a whole mount of the wing?). Second, I would
 like to demonstrate the mechanoreceptor make-up of the tiny hairs on the
 wing membrane.

 Bat wings are highly elastic, with numerous folds, a thickness of about
 35-45 microns (in the species I study), a network of thin collagen bundles,
 and pigmented superficial epidermal layers.
 I could provide more information if required.

 Hoping to hear back from the members.
 Thank you.
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[Histonet] MMP9 and MMP13 on pig tissue

2010-07-01 Thread Reuel Cornelia
Does anybody out there working on MMP9 and 13 on Pig tissue. Where do you 
purchase your antibody. Thank you.



Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768



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RE: [Histonet] control tissue for Hall's bile stain

2010-07-01 Thread Gessford, Mary
Newcomer Supply has bile/liver control slides

newcomersupply.com
cat.#4060A set of ten, 4060B set of 98



Mary Gessford
Senior Scientist
Supervisor - Anatomic Pathology
Toxicology and Pathology Scientific Operations
T: 908-473-4358
MERCK, Summit, NJ
mary.gessf...@spcorp.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
beth.villarr...@novartis.com
Sent: Thursday, July 01, 2010 01:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] control tissue for Hall's bile stain

Does anyone know a commercial source for control tissue for
bile/bilirubin?  Species does not matter.

Thanks!

Beth Villarreal, HT(ASCP)
Novartis Institutes for BioMedical
PCS, Discovery Pathology, US
USCA, 611-7104B
Novartis Institutes for BioMedical
Research, Inc.
100 Technology Square
Cambridge, MA 02139
USA
Phone: +1 617 8714725
Email : beth.villarr...@novartis.com
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[Histonet] Charging question

2010-07-01 Thread Maray Weirauch
A long time ago I attended a pathology charging workshop with Dennis Paget.  He 
had stated that even though tubes and ovaries are a 'go-with' to the uterus, if 
they were submitted for frozen section for separate evaluation they could be 
charged.
We have a case now that included uterus with metastatic carcinoma, both ovaries 
with serous carcinoma, both fallopian tubes with metastatic serous carcinoma 
and pelvic nodes free of involvement.
Our pathologist believes we should charge 88309 (uterus), 88307 x2 (ovaries), 
88305 x2 (fallopian tubes), 88307 (lymph node resection), and the frozen 
section charges.
 I'm not sure the tubes can support a separate charge.  Any comments on this?
Thanks in advance!
Maray


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[Histonet] Cap Question: 2 identifiers for slides

2010-07-01 Thread Scott, Allison D
Is is a CAP requirement to have 2 identifiers on surgical slides?

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
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[Histonet] RE: Cap Question: 2 identifiers for slides

2010-07-01 Thread Nails, Felton
Not yet 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, July 01, 2010 2:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cap Question: 2 identifiers for slides

Is is a CAP requirement to have 2 identifiers on surgical slides?

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
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[Histonet] RE: Cap Question: 2 identifiers for slides

2010-07-01 Thread Weems, Joyce
Joint Commission does... Use case number and name. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton
Sent: Thursday, July 01, 2010 16:10
To: 'Scott, Allison D'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cap Question: 2 identifiers for slides

Not yet 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, July 01, 2010 2:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cap Question: 2 identifiers for slides

Is is a CAP requirement to have 2 identifiers on surgical slides?

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender 
by return e-mail and delete this e-mail and any attachments from your computer 
system.

To the extent the information in this e-mail and any attachments contain 
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Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or 
Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under Texas 
law.  The e-mail is for the use of only the individual or entity named above.  
If you are not the intended recipient, or any authorized representative of the 
intended recipient, you are hereby notified that any review, dissemination or 
copying of this e-mail and its attachments is strictly prohibited.

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