[Histonet] shopping is online.
Discover the latest in women's and men's fashion from brand new cheap goods online with. www.shopbei.com With over 35,000 products from 46 brands, ROBBUY brings you the best in fashion online. New In: Clothing, Shoes(Sport shoes, casual shoes,leather shoes,high heels), Handbags, T shirts, Watches, Jewelry Shoppers can also save money by 1. Register as the VIP member, get 15% discount. It is free to be our member. 2. For the promotion goods, can get 20% discount. Shop here for thousands of cheap brand products from www.shopbei.com, it's safe easy! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions?
Hi Tanisha, I think the question might be...who's requirements are you referring to. I see by the header you reference NY state. I see by your signature that you are in Indiana. I am not aware of any CAP reg referencing this and, to my knowledge most labs have their own job descriptions written with their own qualifications listed. You say ...right now I'm being told... so again, by who (whom). You also reference a BA, I'm assuming this means BS or BAS works as well. I personally know very good supervisors that are associate degreed and hold HTs. I also know supervisors that have bachelor's degrees and I wouldn't necessarily say that they are any better because of it, however I think that's a truism across a lot of occupations. Just trying to understand your question. Have a good day. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Neely, Tanisha Sent: Friday, July 30, 2010 7:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 http://www.covance.com/ - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] staining charged slides
Should charged slides be used for 25 micron sections going thru Nissl stain process? I have used this process on 50 micron sections mounted on gelatin-subbed slides and it worked fine. This time the investigator wanted thinner slides which promptly fell off in the staining dish!! What alternatives do I have for processing this tissue? HELP ! Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] problems with FISH
Hello all. I am encountering a smudgy/milky apearance to my urovision FISH slides.not all in the batch are that way..I know my temps and times were all go and have pH reagents again the only variables I can not acct for are 1. my alcohol. Suppose to use 100% Ethanol w/ no methanol in it.vendor sent reagent grade anhydrous and 2. maybe did not incorporate agitation ? any help or advice would be greatly appreciated. -- K. Leigh Adams, MS, HTL (ASCP),SLS Tennessee Urology Associates 800 Oak Ridge TPKE/STE A206 Oak Ridge, TN 37830 (865) 483-1800 kadams...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: anti-Ly-6G (aka GR-1 ) IHC question
Resending - Does anyone have experience with any anti-mouse LY-6G antibodies on FFPE mouse tissue for staining neutrophils?? Looking for antibodies and protocol suggestions. Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] strontium vs. calcium in bone
Hi, Everyone, Happy Friday! I was wondering if anyone knows of a histological method to distinguish strontium in bone vs. calcium? My understanding is that mineral stains (von Kossa or Alizarin Red S) do not distinguish, but was wondering if anyone has a procedure I may not have done or seen? I have ways to get at the answer, but for completeness sake, I thought I should ask the experts. Thanks for your help! Sincerely, Nicole Collette LLNL/ UC Berkeley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW: anti-Ly-6G (aka GR-1 ) IHC question
Brett We have not used that antibody but we have used the following antibody from Serotec with good success. Protocol: Mouse Neutrophil Immunohistochemical Stain Clone: 7/4 Vendor: Serotec Catalog Number: MCA771 Species: Mouse Isotype: Rat IgG2a Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, July 30, 2010 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: anti-Ly-6G (aka GR-1 ) IHC question Resending - Does anyone have experience with any anti-mouse LY-6G antibodies on FFPE mouse tissue for staining neutrophils?? Looking for antibodies and protocol suggestions. Thanks, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Coverslipping Problem - Please Help!
Hi, Brian, It is likely to be a pH issue. If you use BM Purple as your detection chromogen (which is what we use in our lab for whole mounts on X. laevis and X.tropcialis, and mouse embryos for WMISH), then you must keep the embryos at acid pH from BM Purple step onwards, or you will re-activate the BM Purple (hint: Alkaline phosphatase substrate). Light from the microscope compounds your problem. You are likely cryoprotecting and/or coverslipping with neutral or slightly alkaline solution, if you use something acidic, you should be fine. You can adjust the pH of your sucrose pretty readily, so that's not too hard. If you are only coverslipping temporarily for photos (not for archiving), you can simply use a glycerol/PBS pH 5.5 mixture for photos, then discard your slides. Coverslipping for archiving might be a question better addressed my someone else on histonet who is more knowledgeable about mounting media. Good luck! Sincerely, Nicole Collette LLNL/UC Berkeley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brian Rabe Sent: Thursday, July 29, 2010 3:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipping Problem - Please Help! Dear Histonet members, I have been cryosectioning Xenopus laevis embryos this summer that have undergone a whole mount chromogenic in situ. The embryos are then fixed overnight in a formaldehyde solution (1X MEMFA) at 4 C, then stored in 1 x PBS until they are cryoprotected in a sucrose solution at least overnight at 4 C. Then they are put in TBS tissue freezing medium for 2-3 hours at room temperature before being frozen and sectioned. The sections come out looking very nice with good morphology, but when they are coverslipped (2 1 minute washes in 1 x PBS to dissolve the tissue freezing medium) with VectaMount AQ aqueous mounting medium, the sections turn very dark under the microscope, especially the gut region. They are so dark that it becomes difficult to see the signal from the in situ. Any help or advice would be GREATLY appreciated! Thank you for your time, Brian Rabe The College of William and Mary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://*lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions?
Perhaps someone is thinking about CLIA for which the pathologist meets the requirements for supervisor because they do the diagnosing. Most facilities set their own requirements. which would most likely require an HT (HTL preferred) and so many years experience, supervisory experience preferred. This allows for more flexibility in hiring. Hope this helps, Joyce From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Neely, Tanisha [tanisha.ne...@covance.com] Sent: Friday, July 30, 2010 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 http://www.covance.com/ - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 80, Issue 36
Q Mark Hocking Symphony Technical Applications Specialist Ventana Medical Systems, Inc. A member of the Roche group Message sent via blackberry - Original Message - From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Fri Jul 30 13:02:57 2010 Subject: Histonet Digest, Vol 80, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Reticulum stain wash-offs (Cheryl Crowder) 2. shopping is online. (Kelly Larson) 3. Histology Lab Supervisor Requirements - New York State/CAP Regula tions? (Neely, Tanisha) 4. RE: Histology Lab Supervisor Requirements - New York State/CAP Regula tions? (Thomas Jasper) 5. staining charged slides (Dianne E. Holmes) -- Message: 1 Date: Thu, 29 Jul 2010 23:51:48 -0500 From: Cheryl Crowder ccrow...@vetmed.lsu.edu Subject: [Histonet] Reticulum stain wash-offs To: histonet@lists.utsouthwestern.edu Message-ID: wc20100730045148.652...@vetmed.lsu.edu Content-Type: text/plain; charset=us-ascii Rather than use Halt (or other adhesive) in your water bath, have you tried using charged slides - those used for IHC. Then air-dry or heat dry the tissues before deparaffinizing. Any water left in the tissue will cause wash-offs. Do not lower the pH of the silver solution. Cheryl Crowder, BA, HTL(ASCP) -- Message: 2 Date: Fri, 30 Jul 2010 07:49:53 -0400 From: Kelly Larson kfine...@hotmail.com Subject: [Histonet] shopping is online. To: histonet@lists.utsouthwestern.edu Message-ID: blu141-w18cc4f081763cd27a89632ce...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Discover the latest in women's and men's fashion from brand new cheap goods online with. www.shopbei.com With over 35,000 products from 46 brands, ROBBUY brings you the best in fashion online. New In: Clothing, Shoes(Sport shoes, casual shoes,leather shoes,high heels), Handbags, T shirts, Watches, Jewelry Shoppers can also save money by 1. Register as the VIP member, get 15% discount. It is free to be our member. 2. For the promotion goods, can get 20% discount. Shop here for thousands of cheap brand products from www.shopbei.com, it's safe easy! -- Message: 3 Date: Fri, 30 Jul 2010 10:02:24 -0400 From: Neely, Tanisha tanisha.ne...@covance.com Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? To: histonet@lists.utsouthwestern.edu Message-ID: 816e3c72f855f14985fc31d7c963ae6f205a6...@indexch03.ent.covance.com Content-Type: text/plain; charset=us-ascii Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 http://www.covance.com/ - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. -- Message: 4 Date: Fri, 30 Jul 2010 08:59:20 -0700 From: Thomas Jasper tjas...@copc.net Subject: RE: [Histonet] Histology Lab Supervisor Requirements - New YorkState/CAP Regula tions? To: Neely, Tanisha tanisha.ne...@covance.com Cc: histonet@lists.utsouthwestern.edu Message-ID: 90354a475b420441b2a0396e5008d49692b...@copc-sbs.copc.local Content-Type: text/plain;
[Histonet] Re: Histonet Digest, Vol 80, Issue 36
Q Mark Hocking Symphony Technical Applications Specialist Ventana Medical Systems, Inc. A member of the Roche group Message sent via blackberry - Original Message - From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Fri Jul 30 13:02:57 2010 Subject: Histonet Digest, Vol 80, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Reticulum stain wash-offs (Cheryl Crowder) 2. shopping is online. (Kelly Larson) 3. Histology Lab Supervisor Requirements - New York State/CAP Regula tions? (Neely, Tanisha) 4. RE: Histology Lab Supervisor Requirements - New York State/CAP Regula tions? (Thomas Jasper) 5. staining charged slides (Dianne E. Holmes) -- Message: 1 Date: Thu, 29 Jul 2010 23:51:48 -0500 From: Cheryl Crowder ccrow...@vetmed.lsu.edu Subject: [Histonet] Reticulum stain wash-offs To: histonet@lists.utsouthwestern.edu Message-ID: wc20100730045148.652...@vetmed.lsu.edu Content-Type: text/plain; charset=us-ascii Rather than use Halt (or other adhesive) in your water bath, have you tried using charged slides - those used for IHC. Then air-dry or heat dry the tissues before deparaffinizing. Any water left in the tissue will cause wash-offs. Do not lower the pH of the silver solution. Cheryl Crowder, BA, HTL(ASCP) -- Message: 2 Date: Fri, 30 Jul 2010 07:49:53 -0400 From: Kelly Larson kfine...@hotmail.com Subject: [Histonet] shopping is online. To: histonet@lists.utsouthwestern.edu Message-ID: blu141-w18cc4f081763cd27a89632ce...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Discover the latest in women's and men's fashion from brand new cheap goods online with. www.shopbei.com With over 35,000 products from 46 brands, ROBBUY brings you the best in fashion online. New In: Clothing, Shoes(Sport shoes, casual shoes,leather shoes,high heels), Handbags, T shirts, Watches, Jewelry Shoppers can also save money by 1. Register as the VIP member, get 15% discount. It is free to be our member. 2. For the promotion goods, can get 20% discount. Shop here for thousands of cheap brand products from www.shopbei.com, it's safe easy! -- Message: 3 Date: Fri, 30 Jul 2010 10:02:24 -0400 From: Neely, Tanisha tanisha.ne...@covance.com Subject: [Histonet] Histology Lab Supervisor Requirements - New York State/CAP Regula tions? To: histonet@lists.utsouthwestern.edu Message-ID: 816e3c72f855f14985fc31d7c963ae6f205a6...@indexch03.ent.covance.com Content-Type: text/plain; charset=us-ascii Hi Histonetters: Our HR/QA team is struggling to understand exactly what the requirements are for Histology Technicians/Technologists to become laboratory supervisor. It is my understanding that the requirements are not exactly the same as those for the traditional clinical lab. And until recently, the regulations were vague regarding our field. Right now, I am being told that to be supervisor requires a BA degree and 6 years of experience subsequent to receiving that degree. I am not sure that is accurate. If anyone has any information they can share, I would greatly appreciate it. Thanks, Tanisha N. Neely, HT (ASCP) Global Histology Technical Liaison Covance CLS | 8211 SciCor Drive | Indianapolis, IN 46214 http://www.covance.com/ - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. -- Message: 4 Date: Fri, 30 Jul 2010 08:59:20 -0700 From: Thomas Jasper tjas...@copc.net Subject: RE: [Histonet] Histology Lab Supervisor Requirements - New YorkState/CAP Regula tions? To: Neely, Tanisha tanisha.ne...@covance.com Cc: histonet@lists.utsouthwestern.edu Message-ID:
[Histonet] Slide Imaging/Digital Pathology
Would anyone be willing to share their procedures/protocols for slide imaging/digital pathology as it relates to CAP checklist questions? Any help would be appreciated. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlas...@pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Parvovirus B19
Wondering if anyone is running Cellmarques Parvovirus B19 or Toxoplasma Gondii on a Ventana platform and would you mind sharing your protocol? Have a great weekend everyone! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] filing unstained slides
To All, Just wanted to know how other labs are filing their unstained slides?? If filing along with stained coverslipped slides wouldn't the sections on the unstained slides get scratched?? Any input is appreciated. Thanks in advance, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] filing unstained slides
We file them along with the regular HE usually used when we have send out or re-cut requests. They rarely get scratched (but you must handle them with care). Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 (707) 294-9229 cell- anytime -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Friday, July 30, 2010 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] filing unstained slides To All, Just wanted to know how other labs are filing their unstained slides?? If filing along with stained coverslipped slides wouldn't the sections on the unstained slides get scratched?? Any input is appreciated. Thanks in advance, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Adenovirus controls
I am looking for adenovirus controls, would anyone know where I could get some? I have checked Newcomer and ThermoFisher and they are out. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlas...@pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 80, Issue 36/Reticulin Stain wash-offs
Date: Friday 7/30/10 From: K Ryan _ryandk...@aol.com_ (mailto:ryandk...@aol.com) Subject: [Histonet] Reticulum stain wash-offs To: _histo...@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) I found that shortening the time in the potassium permanganate solved my tissue wash off problem. I was staining liver sections cut at 4 microns on plusslides. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opportunities
Sterling Pathology located in Seal Beach California is currently seeking a Full Time Histotech and (2) Lab Assistants for Part Time or per diem. Histotech should be ASCP Certified with at least 3 years experience preferably proficient in immunos. Lab Assistants may have histology experience preferably with flow cytometry and cytogenetic but will consider applicants with no lab experience. For immediate consideration please email resume to jackie.p...@sterlingpath.com or fax to (562) 799.5544. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
