[Histonet] Dewaxing problem
Dear Histonetters, I've recently started having a problem with tissue sections stubbornly refusing to de-wax. I've been using 2 x 5 minutes in Histoclear to dewax before re-hydrating and staining with normal HE. Until about three weeks ago this process worked fine. Leaving the sections in the Histoclear for longer seems to have little effect and using Xylene instead hasn't done much either - it's taking up to two days or more. The type of tissue seems to be irrelevant, the sections are all cut at 5 microns, and I haven't changed any part of the processing. I've used fresh alcohols and solvents, clean glassware - everything I can think of. Can anyone give me any more ideas or possible causes? because i'm stumped L Thanks Ceri Allen Research Technician School of Veterinary Medicine and Science Sutton Bonnington Campus University of Nottingham Tel. 0115 95 16459 ceri.al...@nottingham.ac.uk This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] searching for pathology dictation system
We still use dictaphones for gross dictation. Does anyone have a system you can recommend? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] searching for pathology dictation system
We use Start Stop with out much problem. www.startstop.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mwh...@mcleodhealth.org Sent: Thursday, August 05, 2010 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] searching for pathology dictation system We still use dictaphones for gross dictation. Does anyone have a system you can recommend? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] searching for pathology dictation system
Melanie, We are using a voice recognition system that interfaces with our LIS. We are using Dragon with a Voice Brook interface. Voice Brook is a pathology specific system that really enhances and picks up on the most challenging medical terminology. We use this for Gross, Pathologist Dictation and Autopsy and have had a great deal of success with it. The PA and Pathologist edit their own dictation as they go along so that when they are finished the report is ready to be finagled. Everything is standardized and template driven. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mwh...@mcleodhealth.org Sent: Thursday, August 05, 2010 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] searching for pathology dictation system We still use dictaphones for gross dictation. Does anyone have a system you can recommend? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] best myoepithelial cell marker for salivary gland
Hi 'netters, One of our pathologists has asked me to find out (in my spare time!) what the best myoepithelial cell marker is for salivary gland. Any opinions? Thanks. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GM of Pathology Ops Job Opportunity
Dear Histonet Subscribers, I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few histotechnician, histotechnologist, and cytotechnologist openings across the country. Our clients typically assist with relocation expenses. One particular client I am working with is looking for a General Manager of Anatomic Pathology Operations for an anatomic pathology lab in NJ. This laboratory is known for its cutting edge technology, LIS system, and its automation. They are looking for someone with 10-20+ years experience in histology/pathology, preferably an HTL(ASCP) certification, at least 10+ years experience in management/operations, and someone who is a strong leader with excellent communication skills. This is a high level pathology position and offers an excellent compensation and benefits package. If interested, please email me your resume to ali...@ka-recruiting.com. Below is a list of some of the opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current HT and HTL Opportunities: 1. Histology Manager - MI 2. Histology Supervisor - NV 3. Histology Supervisor (must have HTL) - Atlanta, GA 4. Histology Supervisor - Central GA 5. Histotech - NYC, NY 6. Grossing Tech - NYC, NY 7. Histotech - IN 8. Histotech - SC 9. Histotech - MA 10. Pathologist's Assistant - NV 11. Pathologist's Assistant - Maine If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microscope Slide stamp
Hi Jill, I know this was a while ago but if you are still in need of something to help label your slides we are carrying an Ultra Fine Lab Marker that works really well on the frosted ends of slides. It is resistant to alcohol and xylene, and writes very nicely on smaller areas. Feel free to check us out at www.sensorhealth.com http://www.sensorhealth.com/ for more details and let us know if you would like to try a free sample. Adam Harris Sales Associate Sensor Health Inc. 110-6 Turnbull Crt. Cambridge, ON N1T 1K6 T: 1-888-777-7080 T: 519-621-1515 F: 519-621-8778 www.sensorhealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Autopsy charge
If you do an autopsy on a patient who has died at home (with no connection to your medical institution) at the request of the family, what do you charge? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PEN SLIDES FOR LASER CAPTURE
Does anyone have any experience with PEN membrane slides from Zeiss for laser capture microdissection. I have been working with one of our residents and we are having problems with the tissue staying on these slides. We could sure use help. Thanks Mary Lloyd ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Microm HM 505 E Cryostat
hello, has anyone with this particular cryostat experienced frost build up on the top interior area located above the microtome? I've also noticed the temperature has for the past couple of weeks been reading ~ 3 - 4 C colder than temperature setting. Thus far it seems to still be sectioning just fine. I'm just curious to know if this is an indication of a malfunction of some sort? I also need to mention that on Monday morning I also observed quite a bit of water on the floor of the lab where this instrument is housed and it's source has yet to be determined. Any pointers will be much appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Autopsy charge
The charge is usually given to the family of the deceased. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Richard Cartun rcar...@harthosp .org To Sent by: Histonet histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Autopsy charge 08/05/2010 12:06 PM If you do an autopsy on a patient who has died at home (with no connection to your medical institution) at the request of the family, what do you charge? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Autopsy charge
For outside cases, we charge $1200 for full autopsy, including brain, $950 for full autopsy not including brain and $700 for a partial autopsy (chest only, abdomen only, brain only). Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, August 05, 2010 12:07 PM To: Histonet Subject: [Histonet] Autopsy charge If you do an autopsy on a patient who has died at home (with no connection to your medical institution) at the request of the family, what do you charge? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 80, Issue 37
Hi Mighnon Did you get a response to your request. I may be able to send you some. Please contact me at mark.elliott(at)hli.ubc.ca Mark Date: Fri, 30 Jul 2010 15:25:00 -0500 From: Mighnon Lashus mlas...@pathgroup.com Subject: [Histonet] Adenovirus controls To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 197cd0b02a81f94994a285c59c8ae05c05ce72a...@pgnexchange.pathgroup.com Content-Type: text/plain; charset=us-ascii I am looking for adenovirus controls, would anyone know where I could get some? I have checked Newcomer and ThermoFisher and they are out. Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlas...@pathgroup.com ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Turnaround time for Research Pathology
Folks, I am looking for some standard turnaround times for histology services including processing to embedding, processing to HE, and processing to HE and IHC. Does anyone have that data? Also, how do other folks handle standard turnaround time for research samples? We may get anything from 5 specimens to 300 specimens that just need paraffin processing and 1 HE. Or we may get a request for 10 samples for paraffin-processing, 10 slides each with 2 HE and 7 different IHC stains per case. Any insight on this would be very helpful. Thanks, Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Turnaround time for Research Pathology
Julie, I've worked in both the clinical and research environments, and the turnaround time depends on which you're talking about. 200-400 blocks a day with about 50% multiple levels and unstained slides, 30-70 immunos, and one or two dozen special stains is a normal day at the hospital lab I'm currently working at. That's a less than 24 hour turn around time for four busy histotechs. On the other hand, for most research labs I've worked at, the turnaround time would be whenever. Sounds to me like your PI's are wanting their stuff out faster. Maybe you are understaffed. Don't expect much sympathy from clinical lab folks. Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
